r-bioc-genomicranges 1.22.0-1 / usr / lib / R / site-library / GenomicRanges / NEWS

CHANGES IN VERSION 1.22.0
-------------------------

NEW FEATURES

    o Support coercions back and forth between a GRanges object and a character
      vector (or factor) with elements in the format 'chr1:2501-2800' or
      'chr1:2501-2800:+'.

    o Add facilities for manipulating "genomic variables": bindAsGRanges(),
      mcolAsRleList(), and binnedAverage(). See ?genomicvars for more
      information.

    o Add "narrow" method for GRangesList objects.

    o Enhancement to the GRanges() constructor. If the 'ranges' argument is not
      supplied then the constructor proceeds in 2 steps:
        1. An initial GRanges object is created with 'as(seqnames, "GRanges")'.
        2. Then this GRanges object is updated according to whatever other
           arguments were supplied to the call to GRanges().
      Because of this enhancement, GRanges(x) is now equivalent to
      'as(x, "GRanges")' e.g. GRanges() can be called directly on a character
      vector representing ranges, or on a data.frame, or on any object for
      which coercion to GRanges is supported.

    o Add 'ignore.strand' argument to "range" and "reduce" methods for
      GRangesList objects.

    o Add coercion from SummarizedExperiment to RangedSummarizedExperiment
      (also available via updateObject()). See 1st item in DEPRECATED AND
      DEFUNCT section below for more information about this.

    o GNCList objects are now subsettable.

    o "coverage" methods now accept 'shift' and 'weight' supplied as an Rle.

SIGNIFICANT USER-LEVEL CHANGES

    o Modify behavior of "*" strand in precede() / follow() to mimic
      'ignore.strand=TRUE'.

    o Revisit "pintersect" methods for GRanges#GRanges, GRangesList#GRanges,
      and GRanges#GRangesList:
      - Sanitize their semantic.
      - Add 'drop.nohit.ranges' argument (FALSE by default).
      - If 'drop.nohit.ranges' is FALSE, the returned object now has a "hit"
        metadata column added to it to indicate the elements in 'x' that
        intersect with the corresponding element in 'y'.

    o binnedAverage() now treats 'numvar' as if it was set to zero on genomic
      positions where it's not set (typically happens when 'numvar' doesn't
      span the entire chromosomes because it's missing the trailing zeros).

    o GRanges() constructor no more mangles the names of the supplied metadata
      columns (e.g. if the column is "_tx_id").

    o makeGRangesFromDataFrame() now accepts "." in strand column (treated as
      "*").

    o GNCList() constructor now propagates the metadata columns.

    o Remove "seqnames" method for RangesList objects.

DEPRECATED AND DEFUNCT

    o The SummarizedExperiment class defined in GenomicRanges is deprecated
      and replaced by 2 new classes defined in the new SummarizedExperiment
      package: SummarizedExperiment0 and RangedSummarizedExperiment.
      In BioC 2.3, the SummarizedExperiment class will be removed from the
      GenomicRanges package and the SummarizedExperiment0 class will be renamed
      SummarizedExperiment. To facilitate this transition, a coercion method
      was added to coerce from old SummarizedExperiment to new
      RangedSummarizedExperiment (this coercion is performed when calling
      updateObject() on an old SummarizedExperiment object).

    o makeSummarizedExperimentFromExpressionSet() and related stuff was moved
      to the new SummarizedExperiment package.

    o After being deprecated in BioC 3.1, the rowData accessor is now defunct
      (replaced with the rowRanges accessor).

    o After being deprecated in BioC 3.1, GIntervalTree objects and the
      "intervaltree" algorithm in findOverlaps() are now defunct.

    o After being deprecated in BioC 3.1, mapCoords() and pmapCoords() are
      now defunct.

BUG FIXES

    o 2 tweaks to subsetting *by* an GenomicRanges:
      - Improve speed when the object to subset is a SimpleList (e.g.
        SimpleRleList).
      - Fix issue when the GenomicRanges subscript is empty.


CHANGES IN VERSION 1.20.0
-------------------------

NEW FEATURES

    o Add coercion methods to go back and forth between ExpressionSet and
      SummarizedExperiment.

    o Add 'assayNames', 'assayNames<-' for SummarizedExperiment

    o assays() supports arrays of up to 4 dimensions.

    o Add GNCList() for preprocessing a GenomicRanges object into a GNCList
      object that can be used for fast overlap seach with findOverlaps().
      GNCList() is a replacement for GIntervalTree() that uses Nested
      Containment Lists instead of interval trees. Unlike GIntervalTree(),
      GNCList() supports preprocessing of a GenomicRanges object with
      ranges located on a circular sequence. For a one time use, it's not
      advised to explicitely preprocess the input. This is because
      findOverlaps() or countOverlaps() will take care of it and do a better
      job at it (that is, they preprocess only what's needed when it's needed
      and release memory as they go).

    o All "findOverlaps" methods now support 'select' equal "last" or
      "arbitrary" (in addition to "all" and "first").

    o Add absoluteRanges() and relativeRanges() to transform back and forth
      between absolute and relative genomic ranges.

SIGNIFICANT USER-LEVEL CHANGES

    o Renamed 'rowData' and 'rowData<-' -> 'rowRanges', 'rowRanges<-'.
      Old names still work but are deprecated.

    o Some improvements to makeGRangesFromDataFrame():
      - Improve internal logic used for finding the GRanges columns in the
        input.
      - If 'seqinfo' is not supplied, the seqlevels are now ordered according
        to the output of GenomeInfoDb::rankSeqlevels().
      - Now an attempt is made to turn 'df' into a data frame (with
        'as.data.frame(df)') if it's not a data frame or a DataFrame object.

    o The GRanges() constructor now propagates the metadata cols that are on
      'ranges' if no metadata cols are explicitly passed to the constructor.

DEPRECATED AND DEFUNCT

    o Deprecated 'rowData' and 'rowData<-' in favor of 'rowRanges' and
      'rowRanges<-'.

    o Deprecated mapCoords() and pmapCoords(). They're replaced by
      mapToTranscripts() and pmapToTranscripts() from the GenomicFeatures
      package and mapToAlignments() and pmapToAlignments() from the
      GenomicAlignments package.

    o Deprecated GIntervalTree objects.

    o Removed "map" and "splitAsListReturnedClass" methods (were defunct in
      GenomicRanges 1.18.0).

    o Removed makeSeqnameIds() (was defunct in GenomicRanges 1.18.0).

    o Removed 'with.mapping' argunment from "reduce" methods (was defunct in
      GenomicRanges 1.18.0).

BUG FIXES

    o Fix 'findOverlaps(..., type="start")' on GRangesList objects which has
      been broken for years.

    o Fix self overlap search on a GRanges object when 'ignore.strand=TRUE'
      (i.e. 'findOverlaps(gr, ignore.strand=TRUE)').


CHANGES IN VERSION 1.18.0
-------------------------

NEW FEATURES

    o Add 'use.mcols' arg to "ranges" method for GRangesList objects. 

    o "assays<-" methods may be invoked with 'withDimnames' arg.

    o Add mapCoords() generic and methods (replacing map()).

    o Add granges,GenomicRanges method. 

    o Add strand<-,GRangesList,character method for global replacement
      (i.e., all strands become 'value'). 

    o Add resize,GRangesList-method.

    o Add DelegatingGenomicRanges class and vignette on how to extend
      GenomicRanges.

    o Document subsetting a named list-like object by a GRanges subscript.

SIGNIFICANT USER-LEVEL CHANGES

    o Modify "show" methods for GRanges and GRangesList objects so
      they print a 1-line summary of the seqinfo component.
      
    o Remove as.data.frame,GRangesList-method; use as.data.frame,List.

    o "trim" method for GenomicRanges only trims out-of-bound
      ranges on non-circular sequences whose length is not NA.
      This behavior is consistent with the GenomicRanges validity method.

    o Changes to flank(), resize() and start/end/width setters:
      - no longer trim the result ranges when called on a GRanges
      - warning is issued by GenomicRanges validity method when
        out-of-bound ranges are on non-circular sequences whose
        length is not NA
      Note this behavior is now consistent with that of shift().

    o Speed up validation of GenomicRanges objects by 1.2x. 

    o Speed up trim() on GenomicRanges objects by 1.2x. 

    o Improve warning when GenomicRanges object contains out-of-bound ranges. 

    o Work on vignette HOWTOs:
      - split 'How to read BAM files into R' into 3 HOWTOs
      - split 'How to prepare a table of read counts for RNA-Seq
        differential gene expression' into 3 HOWTOs
      - split 'How to extract DNA sequences of gene regions' into 2 HOWTOs 
      - make individual HOWTOs subsections of single HOWTO section

    o Follow renaming of TranscriptDb class to TxDb. 

    o Replace references to plantsmart21 with plantsmart22.

DEPRECATED AND DEFUNCT

    o Defunct map() (skip deprecation). Replace with mapCoords().

BUG FIXES

    o [cr]bind,SummarizedExperiment methods respect derived classes.

    o assays(se, withDimnames=TRUE) <- value no longer tries to access
      a slot 'withDimnames'.

    o cbind and rbind,SummarizedExperiment-methods respect derived
      classes

    o "ranges" method for GRangesList objects should not propagate
      inner metadata columns by default.

    o GRanges() constructor now preserves the seqlevels in the order
      supplied by the user.

    o Ensure tileGenome() breakpoints do not extend past end of genome. 

    o Fix "show" method for GenomicRanges objects when 'showHeadLines'
      global option is set to Inf.

    o [rc]bind,SummarizeExperiment-methods now compare all elements.
    
    o Remove "==" and "<=" methods for GenomicRanges objects (not needed). 


CHANGES IN VERSION 1.16.0
-------------------------

NEW FEATURES

    o Add "subset" method for SummarizedExperiment objects.

    o Allow DataFrame in SummarizedExperiment assays.

    o Add 'use.mcols' arg (FALSE by default) to the granges(), grglist(), and
      rglist() generics (a.k.a. the range-squeezer generics).

    o Add coercion method from GRangesList to RangesList.

    o Add score() setter for GRangesList objects.

    o findOverlaps(..., type="within") now works on circular chromosomes.

    o Add 'ignore.strand' arg to "sort" method for GRanges objects.

    o Support subsetting of a named list-like object *by* a GenomicRanges
      subscript.

    o Support sort(granges, by = ~ score), i.e., a formula-based interface for
      sorting by the mcols.

SIGNIFICANT USER-LEVEL CHANGES

    o Move many functionalities to the new GenomicAlignments package:
      - The GAlignments, GAlignmentPairs, and GAlignmentsList classes.
      - The qnarrow() generic and methods.
      - The "narrow" and "pintersect" methods for GAlignments and
        GAlignmentsList objects.
      - The low-level CIGAR utilities.
      - The "findOverlaps" methods for GAlignment* objects.
      - The summarizeOverlaps() generic and methods, and the "Counting reads
        with summarizeOverlaps" vignette.
      - findCompatibleOverlaps() and countCompatibleOverlaps().
      - The findSpliceOverlaps() generic and methods.
      - The "overlap encodings" stuff i.e. the "encodeOverlaps" method for
        GRangesList objects, flipQuery(), selectEncodingWithCompatibleStrand(),
        isCompatibleWithSplicing(), isCompatibleWithSkippedExons(),
        extractSteppedExonRanks(), extractSpannedExonRanks(),
        extractSkippedExonRanks(), and extractQueryStartInTranscript(), and
        the "OverlapEncodings" vignette.

    o Rename 'with.mapping' arg -> 'with.revmap' in "reduce" methods. The old
      arg name is still working but deprecated.

    o Move makeSeqnameIds() function to the new GenomeInfoDb package and rename
      it rankSeqlevels(). The old name is still working but deprecated.

    o The "strand" methods now perform stricter checking and are guaranteed
      to always return a factor (or factor-Rle) with the "standard strand
      levels" and no NAs. Or to fail.

BUG FIXES

    o Tweaks and fixes to various "strand" methods:
      - Methods for character vectors and factors do not accept NAs anymore
        (they raise an error).
      - Methods for integer and logical vectors map NAs to * (instead of NA).
      - Method for Rle objects now also works on character-, factor-, and
        integer-Rle objects (in addition to logical-Rle objects).


CHANGES IN VERSION 1.14.0
-------------------------

NEW FEATURES

    o Add coercion from GenomicRangesList to RangedDataList.

    o Add "c" method for GAlignmentPairs objects. 

    o Add coercion from GAlignmentPairs to GAlignmentsList. 

    o Add 'inter.feature' and 'fragment' arguments to summarizeOverlaps().

    o Add seqselect,GAlignments-method.
 
    o Add CIGAR utilities:
      explodeCigarOps(), explodeCigarOpLengths()
      cigarRangesAlongReferenceSpace(), cigarRangesAlongQuerySpace()
      cigarRangesAlongPairwiseSpace(), extractAlignmentRangesOnReference()
      cigarWidthAlongReferenceSpace(), cigarWidthAlongQuerySpace()
      cigarWidthAlongPairwiseSpace().

    o Add seqlevels0() and restoreSeqlevels(). 

    o Add seqlevelsInUse() getter for GRanges, GRangesList, GAlignments
      GAlignmentPairs, GAlignmentsList and SummarizedExperiment objects. 

    o Add update,GAlignments method.
 
    o Add GIntervalTree class.
 
    o Add coercion from GAlignmentPairs to GAlignments.
 
    o Add sortSeqlevels().
 
    o Add tileGenome(). 

    o Add makeGRangesFromDataFrame() and coercion from data.frame or DataFrame
      to GRanges. 

SIGNIFICANT USER-LEVEL CHANGES

    o Renaming (with aliases from old to new names):
      - classes
        GappedAlignments -> GAlignments
        GappedAlignmentPairs -> GAlignmentPairs
      - functions
        GappedAlignments() -> GAlignments()
        GappedAlignmentPairs() -> GAlignmentPairs()
        readGappedAlignments() -> readGAlignments()
        readGappedAlignmentPairs() -> readGAlignmentPairs()

    o Remove 'asProperPairs' argument to readGAlignmentsList(). 

    o Modify "show" method for Seqinfo object to honor showHeadLines
      and showTailLines global options. 

    o 50x speedup or more when merging 2 Seqinfo objects, 1 very small
      and 1 very big. 

    o Add dependency on new XVector package. 

    o Enhanced examples for renaming seqlevels in seqlevels-utils.Rd. 

    o More efficient reference class constructor for 'assays'
      slot of SummarizedExperiment objects. 

    o 'colData' slot of SummarizedExperiment produced from call to
      summarizedOverlaps() now holds the class type and length of 'reads'.

    o 4x speedup to cigarToRleList(). 

    o Relax SummarizedExperiment class validity. 
 
    o Renaming (with aliases from old to new names):
      cigarToWidth() -> cigarWidthOnReferenceSpace(), and 
      cigarToQWidth() -> cigarWidthOnQuerySpace(). 

    o Improvements to summarizeOverlaps():
      - mode 'Union': 1.5x to 2x speedup
      - mode 'IntersectionNotEmpty': 2x to 8x speedup + memory footprint 
        reduced by ~ half

    o Change default 'use.names' to FALSE for readGAlignmentsList().

    o Implement 'type="equal"' for findOverlaps,SummarizedExperiment methods. 

    o Modify summarizeOverlaps() examples to use 'asMates=TRUE' instead
      of 'obeyQname=TRUE'. 

    o Remove unneeded "window" method for GenomicRanges objects. 

    o Speed up seqinfo() getter and setter on SummarizedExperiment objects
      and derivatives (e.g. VCF) by using direct access to 'rowData' slot.

    o coverage,GenomicRanges method now uses .Ranges.coverage() when
      using the defaults for 'shift' and 'width'.

    o Remove restriction that metadata column names must be different on
      a GRangesList and the unlisted GRanges. 

    o GenomicRangesUseCases vignette has been redone and renamed
      to GenomicRangesHOWTOs 

DEPRECATED AND DEFUNCT
 
    o Defunct all "match" and "%in%" methods in the 
      package except for those with the GenomicRanges,GenomicRanges signature.

    o Deprecate GappedAlignment*:
      - GappedAlignments and GappedAlignmentPairs classes
      - GappedAlignments() and GappedAlignmentPairs() constructors
      - readGappedAlignments() and readGappedAlignmentPairs() functions
 
    o Deprecate cigar util functions:
      cigarToWidth(), cigarToQWidth(), cigarToIRanges()
      splitCigar(), cigarToIRanges(), cigarToIRangesListByAlignment()
      cigarToIRangesListByRName(), cigarToWidth(), cigarToQWidth()
      cigarToCigarTable(), summarizeCigarTable()

    o Deprecate seqselect().

BUG FIXES

    o Fix bug in c,GAlignments for case when objects were unnamed. 

    o Fix bug in flank,GenomicRanges (when 'ignore.strand=TRUE'
      'start' was being set to TRUE). 

    o Fix bug in behavior of summarizeOverlaps() count mode 
      'IntersectionNotEmpty' when 'inter.features=FALSE'. Shared 
      regions are now removed before counting. 

    o Fix bug in cigarToIRangesListByAlignment() when 'flag' is
      supplied and indicates some reads are unmapped. 

    o Fix bug in summarizeOverlaps(..., mode='IntersectionNotEmpty')
      when 'features' has '-' and '+' elements and 'ignore.strand=TRUE'. 

    o match,GenomicRanges,GenomicRanges method now handles properly objects
      with seqlevels not in the same order. 


CHANGES IN VERSION 1.12.0
-------------------------

NEW FEATURES

    o Implement "seqnameStyle" replacement method for Seqinfo object.
      'seqnameStyle(x) <- style' works on any object with a "seqinfo"
      replacement method.

    o Add trim,GenomicRanges-method to trim out of bound ranges. 

    o Add promoters,GenomicRanges and promoters,GRangesList methods. 
 
    o Add "overlapsAny" methods as a replacement for the deprecated
      "%in%" methods. 

    o Add 'ignore.strand' argument to match,GenomicRanges-method. 

    o Add 'with.mapping' argument to "reduce" method for GenomicRanges
      objects.

    o Add "unname" method to remove dimnames from SummarizedExperiment. 

    o Add "cbind" and "rbind" methods for SummarizedExperiment. 

    o Add "seqselect", "seqselect<-" and "split" methods for 
      SummarizedExperiment. 

    o Add GAlignmentsList class.
 
    o Add readGAlignmentsList generic and methods. 

SIGNIFICANT USER-LEVEL CHANGES

    o resize,GenomicRanges method no longer checks that 'fix' is 
      length-compatible with 'x' when 'x' is length zero. This
      allows for resize(x, w, fix = "end") without worrying about 
      'x' being zero-length.

    o Change the behavior of "distance". Previously adjacent ranges
      had a distance of 1 and overlapping had a distance of 0. Now
      both adjacent AND overlapping have a distance of 0.

    o shift,GenomicRanges-method no longer trims out of bound ranges.

    o "distanceToNearest" no longer drops ranges that have no hit
      but returns 'NA' for 'subjectHits' and 'distance'. 

    o "genome" is no longer an invalid metadata colname for GenomicRanges
      objects.

    o 4x-8x speedup for doing coverage() on a GRanges or GRangesList with 
      many seqlevels.

    o Remove ">=", "<", and ">" methods for GenomicRanges objects.

    o Speedup "seqinfo" setters for GenomicRanges and GappedAlignments
      by avoiding validation when not necessary. 

    o readGappedAlignments can now pass a BamFile to readBamGappedAlignments. 

    o Remove unneeded "unique" and "sort" methods for GenomicRanges objects. 
 
    o Change behavior of "match" and "%in%" on GenomicRanges objects to use
      equality instead of overlap for comparing elements between GenomicRanges
      objects 'x' and 'table'.

    o match,GenomicRanges-method gets the same 'method' argumnet as the 
      "duplicated" method for these objects. 
 
    o Remove unneeded "countOverlaps" methods. 
 
    o "classNameForDisplay" shortens the name of data type when displayed.
 
    o Add global options 'showHeadLines' and 'showTailLines' to
      control the number of head/tails lines displayed in
      show,GRanges and show,GappedAlignments methods. 

    o "distanceToNearest" now returns a Hits object instead of DataFrame.

DEPRECATED AND DEFUNCT

    o Remove defunct countGenomicOverlaps(), grg(), and globalToQuery()

    o Defunct previously deprecated '.ignoreElementMetadata' argmuent
      of c,GenomicRanges-method.
 
    o Deprecate all "match" and "%in%" methods in the package except for
      those with the GenomicRanges,GenomicRanges signature.

    o Deprecate "resolveHits" methods.
 
BUG FIXES

    o Several bug fixes to "nearest". 

    o Output of "findSpliceOverlaps" now displays 'NA' for ranges with 
      no hits.


CHANGES IN VERSION 1.10.0
-------------------------

NEW FEATURES

    o SummarizedExperiment gains direct GRanges / GRangesList
      interface to rowData.

    o Add "distanceToNearest" method for GenomicRanges objects.

    o SummarizedExperiment class can now be subset by row when there are no
      'columns', and by column when there are no 'rows'.

    o Add 'drop.D.ranges' argument to coverage,GappedAlignments and
      coverage,GappedAlignmentPairs methods.

    o findOverlaps() now supports 'select="last"' and 'select="arbitrary"' (in
      addition to 'select="all"' and 'select="first"') on GenomicRanges
      objects.

    o summarizeOverlaps(..., mode="IntersectionStrict") now handles circular
      chromosomes. A warning is issued and circular chromosomes in 'reads' are
      omitted from counting.

    o Add disjoin,GRangesList method.

    o Add findSpliceOverlaps() for identifyng ranges (reads) that are
      compatible with a specific transcript isoform (the non-compatible
      ranges are analyzed for the presence of novel splice events).

    o Add ngap,GappedAlignmentPairs method.

    o Add introns() generic with methods for GappedAlignments and
      GappedAlignmentPairs objects.

    o No more arbitrary max of 3 gaps per read in isCompatibleWithSplicing()
      and isCompatibleWithSkippedExons(). 

    o Add findCompatibleOverlaps() and countCompatibleOverlaps().

    o Passing '...' down through as.data.frame(GRanges, ...) so user can tweak
      stringsAsFactors default for metadata columns.

    o Add extractSteppedExonRanks(), extractSpannedExonRanks() and
      extractQueryStartInTranscript() utilities (work with single- and
      paired-end reads).

    o Add 'flip.query.if.wrong.strand' arg (FALSE by default) to
      "encodeOverlaps" method for GRangesList objects.

    o Add makeSeqnameIds() low-level utility.

SIGNIFICANT USER-LEVEL CHANGES

    o SummarizedExperiment rowData and assays operations have
      significant performance improvements.

    o mcols() is now the preferred way (over elementMetadata() or values()) to
      access the metadata columns of a GenomicRanges, GRangesList,
      GappedAlignments, GappedAlignmentPairs, SummarizedExperiment object,
      or any Vector object. elementMetadata() and values() might go away at
      some point in the (not so close) future.

    o Add "$" and "$<-" methods for GenomicRanges *only*. Provided as a
      convenience and as the result of strong popular demand. Note that those
      methods are not consistent with the other "$" and "$<-" methods in the
      IRanges/GenomicRanges infrastructure, and might confuse some users by
      making them believe that a GenomicRanges object can be manipulated as a
      data.frame-like object. It is therefore recommended to use them only
      interactively, and their use in scripts or packages is discouraged.
      For the latter, use 'mcols(x)$name' instead of 'x$name'.

    o No more warning when doing as(x, "GRanges") on a RangedData object with
      no "strand" column.

    o Refactor "[" method for GenomicRanges objects. The new implementation
      always preserves the names of the selected elements instead of trying
      to return a GenomicRanges object with unique names. This new behavior
      is consistent with subsetting of ordinary vectors and other Vector
      objects defined in IRanges/GenomicRanges. Also modify "seqselect" method
      for GenomicRanges objects so it also preserves the names of the selected
      elements (and thus remains consistent with new behavior of "[" method for
      GenomicRanges objects).

    o No more names on the integer vector returned by "ngap" method for
      GappedAlignments objects.

    o Many improvements to the "Overlap encodings" vignette.

    o Remove 'param' argument from summarizeOverlaps() generic.

DEPRECATED AND DEFUNCT

    o Defunct previously deprecated grg() function.

    o Defunct previously deprecated countGenomicOverlaps() generic and methods.

BUG FIXES

    o Fix several issues with "precede", "follow", "nearest", and "distance"
      methods for GenomicRanges objects.

    o Fix bug in summarizeOverlaps(..., ignore.strand=TRUE).

    o 6x speedup (and a 6x memory footprint reduction) or more when using
      encodeOverlaps() on big GRangesList objects.

    o Fix bug in renameSeqlevels() wrt order of rename vector.

    o Fix bug in selectEncodingWithCompatibleStrand().


CHANGES IN VERSION 1.8.0
------------------------

NEW FEATURES

    o Add GappedAlignmentPairs class (with accessors first(), last(), left(),
      right(), seqnames(), strand(), isProperPair()), and
      readGappedAlignmentPairs() for dealing with paired-end reads.
      Most of the GappedAlignments functionalities (e.g. coercion to
      GRangesList, "findOverlaps" and related methods, "coverage", etc...)
      work on a GappedAlignmentPairs object.

    o Add encodeOverlaps,GRangesList,GRangesList,missing and related utilities
      flipQuery(), selectEncodingWithCompatibleStrand(),
      isCompatibleWithSplicing(), isCompatibleWithSkippedExons() and
      extractSkippedExonRanks().

    o Add 'order.as.in.query' arg to grglist() and rglist().

    o SummarizedExperiment gains direct access to colData columns with
      $, $<-, [[, and [[<- methods

    o Add map,GenomicRanges,GRangesList and
      map,GenomicRanges,GappedAlignments methods. These allow mapping
      from genome space to transcript space, and genome space to read
      space, respectively.
    
    o Add seqinfo methods (and friends) for RangedData, RangesList,
      and other IRanges data structures. These use metadata(x)$seqinfo.

    o Add disjointBins,GenomicRanges.

    o Add score,GRangesList and score,GenomicRanges (gets the score column
      like for RangedData).
    
    o Add RangedDataList -> GenomicRangesList coercion.

    o Add RleViewsList -> GRanges coercion.

    o Add pintersect,GRangesList,GRangesList

    o Add stack,GenomicRangesList
    
    o ignore.strand argument now more uniformly supported on set operations.

    o Add Ops,GenomicRanges (from rtracklayer).

    o Add strand,Rle (only logical-Rle is supported).

    o Add compare,GenomicRanges

    o Add 'drop.empty.ranges' arg (FALSE by default) to low-level cigar
      utilities cigarToIRanges(), cigarToIRangesListByAlignment(), and
      cigarToIRangesListByRName().

    o Add 'reduce.ranges' arg to cigarToIRangesListByAlignment().
 
SIGNIFICANT USER-LEVEL CHANGES

    o grglist,GappedAlignments now carries over metadata columns.

    o Names are no longer forced to be unique when unlisting a
      GRangesList with use.names=TRUE.

    o seqnames() is now preferred over rname() on a GappedAlignments object.

    o cigarToIRangesListByAlignment() now returns a CompressedIRangesList
      instead of CompressedNormalIRangesList.

    o Low-level CIGAR utilities now ignore CIGAR operation P (instead of
      trowing an error).

    o The 'weight' arg in "coverage" method for GenomicRanges objects now
      can also be a single string naming a column in elementMetadata(x).

    o Ranges outside the sequences bounds of the underlying sequences are now
      accepted (with a warning) in GenomicRanges/GRangesList/GappedAlignments
      objects.

    o When called with 'ignore.strand=TRUE', the "range" and "disjoin" methods
      for GenomicRanges objects now behave like if they set the strand of the
      input to "*" before they do any computation.

    o When called with 'ignore.strand=TRUE', "reduce" method for GenomicRanges
      objects, and "union", "intersect" and "setdiff" methods for GRanges
      objects now set the strand of their arguments to "*" prior to any
      computation.

    o No more mangling of the names when combining GRanges objects ("c" method
      for GRanges objects was trying to return unique names).

    o Remove isCircularWithKnownLength() generic and methods (nobody knows,
      uses, or needs this).

BUG FIXES

    o flank,GRangesList no longer forces 'use.names' to TRUE and 'both' to
      FALSE.

    o range,GenomicRanges was broken when object had no ranges

    o Fix integer overflow issue that can occur in cigarQNarrow() or
      cigarQNarrow() when the cigar vector is very long.


CHANGES IN VERSION 1.6.0
------------------------

NEW FEATURES

    o seqlevels() and seqinfo() setters have a new arg ('force', default is
      FALSE) to force dropping sequence levels currently in use.

    o Seqinfo objects now have a genome column that can be accessed with
      genome() getter/setter.

    o "pgap" method for c(x="GRanges", y="GRanges").

    o Add comparison (==, <=, duplicated, unique, etc...) and ordering
      (order, sort, rank) methods for GenomicRanges objects.

    o Add "flank" method for GRangesList objects.

    o Add "isDisjoint" and "restrict" methods for GRanges and GRangesList
      objects.

    o Add GRangesList constructor makeGRangesListFromFeatureFragments().

    o Add "names" and "names<-" methods for GappedAlignments objects.

    o Add 'ignore.strand' arg to a number of methods:
        - findOverlaps,GRangesList,RangesList
        - findOverlaps,GappedAlignments,ANY
        - findOverlaps,ANY,GappedAlignments

    o 'shift' and 'weight' arguments of "coverage" method for GenomicRanges
      objects now can be numeric vectors in addition to lists.

    o Add "c" method for GappedAlignments objects.

SIGNIFICANT USER-VISIBLE CHANGES

    o readGappedAlignments() supports 2 new arguments: (1) 'use.names' (default
      is FALSE) for using the query template names (QNAME field in a SAM/BAM
      file) to set the names of the returned object, and (2) 'param' (default
      is NULL, otherwise a ScanBamParam object) for controlling what fields and
      which records are imported. readGappedAlignments() doesn't support
      the 'which' arg anymore.

    o The names of a GRanges/GRangesList/GappedAlignments object are not
      required to be unique anymore.

    o By default, the rownames are not set anymore on the DataFrame returned
      by elementMetadata() on a GRanges/GRangesList/GappedAlignments object.

    o 'width' arg of "coverage" method for GenomicRanges objects now must be
      NULL or numeric vector (instead of NULL or list).

DEPRECATED AND DEFUNCT

    o Deprecate countGenomicOverlaps() in favor of summarizeOverlaps().

    o Deprecate grg() in favor of granges().

BUG FIXES

    o Fix bug in "pintersect" methods operating on GappedAlignments objects.