This file is indexed.

/usr/share/perl5/Bio/Root/IO.pm is in libbio-perl-perl 1.6.924-3.

This file is owned by root:root, with mode 0o644.

The actual contents of the file can be viewed below.

   1
   2
   3
   4
   5
   6
   7
   8
   9
  10
  11
  12
  13
  14
  15
  16
  17
  18
  19
  20
  21
  22
  23
  24
  25
  26
  27
  28
  29
  30
  31
  32
  33
  34
  35
  36
  37
  38
  39
  40
  41
  42
  43
  44
  45
  46
  47
  48
  49
  50
  51
  52
  53
  54
  55
  56
  57
  58
  59
  60
  61
  62
  63
  64
  65
  66
  67
  68
  69
  70
  71
  72
  73
  74
  75
  76
  77
  78
  79
  80
  81
  82
  83
  84
  85
  86
  87
  88
  89
  90
  91
  92
  93
  94
  95
  96
  97
  98
  99
 100
 101
 102
 103
 104
 105
 106
 107
 108
 109
 110
 111
 112
 113
 114
 115
 116
 117
 118
 119
 120
 121
 122
 123
 124
 125
 126
 127
 128
 129
 130
 131
 132
 133
 134
 135
 136
 137
 138
 139
 140
 141
 142
 143
 144
 145
 146
 147
 148
 149
 150
 151
 152
 153
 154
 155
 156
 157
 158
 159
 160
 161
 162
 163
 164
 165
 166
 167
 168
 169
 170
 171
 172
 173
 174
 175
 176
 177
 178
 179
 180
 181
 182
 183
 184
 185
 186
 187
 188
 189
 190
 191
 192
 193
 194
 195
 196
 197
 198
 199
 200
 201
 202
 203
 204
 205
 206
 207
 208
 209
 210
 211
 212
 213
 214
 215
 216
 217
 218
 219
 220
 221
 222
 223
 224
 225
 226
 227
 228
 229
 230
 231
 232
 233
 234
 235
 236
 237
 238
 239
 240
 241
 242
 243
 244
 245
 246
 247
 248
 249
 250
 251
 252
 253
 254
 255
 256
 257
 258
 259
 260
 261
 262
 263
 264
 265
 266
 267
 268
 269
 270
 271
 272
 273
 274
 275
 276
 277
 278
 279
 280
 281
 282
 283
 284
 285
 286
 287
 288
 289
 290
 291
 292
 293
 294
 295
 296
 297
 298
 299
 300
 301
 302
 303
 304
 305
 306
 307
 308
 309
 310
 311
 312
 313
 314
 315
 316
 317
 318
 319
 320
 321
 322
 323
 324
 325
 326
 327
 328
 329
 330
 331
 332
 333
 334
 335
 336
 337
 338
 339
 340
 341
 342
 343
 344
 345
 346
 347
 348
 349
 350
 351
 352
 353
 354
 355
 356
 357
 358
 359
 360
 361
 362
 363
 364
 365
 366
 367
 368
 369
 370
 371
 372
 373
 374
 375
 376
 377
 378
 379
 380
 381
 382
 383
 384
 385
 386
 387
 388
 389
 390
 391
 392
 393
 394
 395
 396
 397
 398
 399
 400
 401
 402
 403
 404
 405
 406
 407
 408
 409
 410
 411
 412
 413
 414
 415
 416
 417
 418
 419
 420
 421
 422
 423
 424
 425
 426
 427
 428
 429
 430
 431
 432
 433
 434
 435
 436
 437
 438
 439
 440
 441
 442
 443
 444
 445
 446
 447
 448
 449
 450
 451
 452
 453
 454
 455
 456
 457
 458
 459
 460
 461
 462
 463
 464
 465
 466
 467
 468
 469
 470
 471
 472
 473
 474
 475
 476
 477
 478
 479
 480
 481
 482
 483
 484
 485
 486
 487
 488
 489
 490
 491
 492
 493
 494
 495
 496
 497
 498
 499
 500
 501
 502
 503
 504
 505
 506
 507
 508
 509
 510
 511
 512
 513
 514
 515
 516
 517
 518
 519
 520
 521
 522
 523
 524
 525
 526
 527
 528
 529
 530
 531
 532
 533
 534
 535
 536
 537
 538
 539
 540
 541
 542
 543
 544
 545
 546
 547
 548
 549
 550
 551
 552
 553
 554
 555
 556
 557
 558
 559
 560
 561
 562
 563
 564
 565
 566
 567
 568
 569
 570
 571
 572
 573
 574
 575
 576
 577
 578
 579
 580
 581
 582
 583
 584
 585
 586
 587
 588
 589
 590
 591
 592
 593
 594
 595
 596
 597
 598
 599
 600
 601
 602
 603
 604
 605
 606
 607
 608
 609
 610
 611
 612
 613
 614
 615
 616
 617
 618
 619
 620
 621
 622
 623
 624
 625
 626
 627
 628
 629
 630
 631
 632
 633
 634
 635
 636
 637
 638
 639
 640
 641
 642
 643
 644
 645
 646
 647
 648
 649
 650
 651
 652
 653
 654
 655
 656
 657
 658
 659
 660
 661
 662
 663
 664
 665
 666
 667
 668
 669
 670
 671
 672
 673
 674
 675
 676
 677
 678
 679
 680
 681
 682
 683
 684
 685
 686
 687
 688
 689
 690
 691
 692
 693
 694
 695
 696
 697
 698
 699
 700
 701
 702
 703
 704
 705
 706
 707
 708
 709
 710
 711
 712
 713
 714
 715
 716
 717
 718
 719
 720
 721
 722
 723
 724
 725
 726
 727
 728
 729
 730
 731
 732
 733
 734
 735
 736
 737
 738
 739
 740
 741
 742
 743
 744
 745
 746
 747
 748
 749
 750
 751
 752
 753
 754
 755
 756
 757
 758
 759
 760
 761
 762
 763
 764
 765
 766
 767
 768
 769
 770
 771
 772
 773
 774
 775
 776
 777
 778
 779
 780
 781
 782
 783
 784
 785
 786
 787
 788
 789
 790
 791
 792
 793
 794
 795
 796
 797
 798
 799
 800
 801
 802
 803
 804
 805
 806
 807
 808
 809
 810
 811
 812
 813
 814
 815
 816
 817
 818
 819
 820
 821
 822
 823
 824
 825
 826
 827
 828
 829
 830
 831
 832
 833
 834
 835
 836
 837
 838
 839
 840
 841
 842
 843
 844
 845
 846
 847
 848
 849
 850
 851
 852
 853
 854
 855
 856
 857
 858
 859
 860
 861
 862
 863
 864
 865
 866
 867
 868
 869
 870
 871
 872
 873
 874
 875
 876
 877
 878
 879
 880
 881
 882
 883
 884
 885
 886
 887
 888
 889
 890
 891
 892
 893
 894
 895
 896
 897
 898
 899
 900
 901
 902
 903
 904
 905
 906
 907
 908
 909
 910
 911
 912
 913
 914
 915
 916
 917
 918
 919
 920
 921
 922
 923
 924
 925
 926
 927
 928
 929
 930
 931
 932
 933
 934
 935
 936
 937
 938
 939
 940
 941
 942
 943
 944
 945
 946
 947
 948
 949
 950
 951
 952
 953
 954
 955
 956
 957
 958
 959
 960
 961
 962
 963
 964
 965
 966
 967
 968
 969
 970
 971
 972
 973
 974
 975
 976
 977
 978
 979
 980
 981
 982
 983
 984
 985
 986
 987
 988
 989
 990
 991
 992
 993
 994
 995
 996
 997
 998
 999
1000
1001
1002
1003
1004
1005
1006
1007
1008
1009
1010
1011
1012
1013
1014
1015
1016
1017
1018
1019
1020
1021
1022
1023
1024
1025
1026
1027
1028
1029
1030
1031
1032
1033
1034
1035
1036
1037
1038
1039
1040
1041
1042
1043
1044
1045
1046
1047
1048
1049
1050
1051
1052
1053
1054
1055
1056
1057
1058
1059
1060
1061
1062
1063
1064
1065
1066
1067
1068
1069
1070
1071
1072
1073
1074
1075
1076
1077
1078
1079
1080
1081
1082
1083
1084
1085
1086
1087
1088
1089
1090
1091
1092
1093
1094
1095
1096
1097
1098
1099
1100
1101
1102
1103
1104
1105
1106
1107
1108
1109
1110
1111
1112
1113
1114
1115
1116
1117
1118
1119
1120
1121
1122
1123
1124
1125
1126
1127
1128
1129
1130
1131
1132
1133
1134
1135
1136
1137
1138
package Bio::Root::IO;

use strict;
use Symbol;
use IO::Handle;
use File::Copy;
use Fcntl;
use base qw(Bio::Root::Root);

# ABSTRACT: module providing several methods often needed when dealing with file IO
# AUTHOR:   Hilmar Lapp <hlapp@gmx.net>
# OWNER:    Hilmar Lapp
# LICENSE:  Perl_5

# CONTRIBUTOR: Mark A. Jensen <maj@fortinbras.us>

=head1 SYNOPSIS

    # Use stream I/O in your module
    $self->{'io'} = Bio::Root::IO->new(-file => "myfile");
    $self->{'io'}->_print("some stuff");
    my $line = $self->{'io'}->_readline();
    $self->{'io'}->_pushback($line);
    $self->{'io'}->close();

    # obtain platform-compatible filenames
    $path = Bio::Root::IO->catfile($dir, $subdir, $filename);
    # obtain a temporary file (created in $TEMPDIR)
    ($handle) = $io->tempfile();

=head1 DESCRIPTION

This module provides methods that will usually be needed for any sort
of file- or stream-related input/output, e.g., keeping track of a file
handle, transient printing and reading from the file handle, a close
method, automatically closing the handle on garbage collection, etc.

To use this for your own code you will either want to inherit from
this module, or instantiate an object for every file or stream you are
dealing with. In the first case this module will most likely not be
the first class off which your class inherits; therefore you need to
call _initialize_io() with the named parameters in order to set file
handle, open file, etc automatically.

Most methods start with an underscore, indicating they are private. In
OO speak, they are not private but protected, that is, use them in
your module code, but a client code of your module will usually not
want to call them (except those not starting with an underscore).

In addition this module contains a couple of convenience methods for
cross-platform safe tempfile creation and similar tasks. There are
some CPAN modules related that may not be available on all
platforms. At present, File::Spec and File::Temp are attempted. This
module defines $PATHSEP, $TEMPDIR, and $ROOTDIR, which will always be set,
and $OPENFLAGS, which will be set if either of File::Spec or File::Temp fails.

The -noclose boolean (accessed via the noclose method) prevents a
filehandle from being closed when the IO object is cleaned up.  This
is special behavior when a object like a parser might share a
filehandle with an object like an indexer where it is not proper to
close the filehandle as it will continue to be reused until the end of the
stream is reached.  In general you won't want to play with this flag.

=cut

our ($FILESPECLOADED,   $FILETEMPLOADED,
     $FILEPATHLOADED,   $TEMPDIR,
     $PATHSEP,          $ROOTDIR,
     $OPENFLAGS,        $VERBOSE,
     $ONMAC,            $HAS_EOL,       );

my $TEMPCOUNTER;
my $HAS_WIN32 = 0;

BEGIN {
    $TEMPCOUNTER = 0;
    $FILESPECLOADED = 0;
    $FILETEMPLOADED = 0;
    $FILEPATHLOADED = 0;
    $VERBOSE = 0;

    # try to load those modules that may cause trouble on some systems
    eval {
        require File::Path;
        $FILEPATHLOADED = 1;
    };
    if( $@ ) {
        print STDERR "Cannot load File::Path: $@" if( $VERBOSE > 0 );
        # do nothing
    }

    # If on Win32, attempt to find Win32 package
    if($^O =~ /mswin/i) {
        eval {
            require Win32;
            $HAS_WIN32 = 1;
        };
    }

    # Try to provide a path separator. Why doesn't File::Spec export this,
    # or did I miss it?
    if ($^O =~ /mswin/i) {
        $PATHSEP = "\\";
    } elsif($^O =~ /macos/i) {
        $PATHSEP = ":";
    } else { # unix
        $PATHSEP = "/";
    }
    eval {
        require File::Spec;
        $FILESPECLOADED = 1;
        $TEMPDIR = File::Spec->tmpdir();
        $ROOTDIR = File::Spec->rootdir();
        require File::Temp; # tempfile creation
        $FILETEMPLOADED = 1;
    };
    if( $@ ) {
        if(! defined($TEMPDIR)) { # File::Spec failed
            # determine tempdir
            if (defined $ENV{'TEMPDIR'} && -d $ENV{'TEMPDIR'} ) {
                $TEMPDIR = $ENV{'TEMPDIR'};
            } elsif( defined $ENV{'TMPDIR'} && -d $ENV{'TMPDIR'} ) {
                $TEMPDIR = $ENV{'TMPDIR'};
            }
            if($^O =~ /mswin/i) {
                $TEMPDIR = 'C:\TEMP' unless $TEMPDIR;
                $ROOTDIR = 'C:';
            } elsif($^O =~ /macos/i) {
                $TEMPDIR = "" unless $TEMPDIR; # what is a reasonable default on Macs?
                $ROOTDIR = ""; # what is reasonable??
            } else { # unix
                $TEMPDIR = "/tmp" unless $TEMPDIR;
                $ROOTDIR = "/";
            }
            if (!( -d $TEMPDIR && -w $TEMPDIR )) {
                $TEMPDIR = '.'; # last resort
            }
        }
        # File::Temp failed (alone, or File::Spec already failed)
        # determine open flags for tempfile creation using Fcntl
        $OPENFLAGS = O_CREAT | O_EXCL | O_RDWR;
        for my $oflag (qw/FOLLOW BINARY LARGEFILE EXLOCK NOINHERIT TEMPORARY/){
            my ($bit, $func) = (0, "Fcntl::O_" . $oflag);
            no strict 'refs';
            $OPENFLAGS |= $bit if eval { $bit = &$func(); 1 };
        }
    }
    $ONMAC = "\015" eq "\n";
}


=head2 new

 Title   : new
 Usage   : my $io = Bio::Root::IO->new( -file => 'data.txt' );
 Function: Create new class instance. It automatically calls C<_initialize_io>.
 Args    : Same named parameters as C<_initialize_io>.
 Returns : A Bio::Root::IO object

=cut

sub new {
    my ($caller, @args) = @_;
    my $self = $caller->SUPER::new(@args);
    $self->_initialize_io(@args);
    return $self;
}


=head2 _initialize_io

 Title   : _initialize_io
 Usage   : $io->_initialize_io(@params);
 Function: Initializes filehandle and other properties from the parameters.
 Args    : The following named parameters are currently recognized:
              -file     name of file to read or write to
              -fh       file handle to read or write to (mutually exclusive
                        with -file and -string)
              -input    name of file, or filehandle (GLOB or IO::Handle object)
                        to read of write to
              -string   string to read from (will be converted to filehandle)
              -url      name of URL to open
              -flush    boolean flag to autoflush after each write
              -noclose  boolean flag, when set to true will not close a
                        filehandle (must explicitly call close($io->_fh)
              -retries  number of times to try a web fetch before failure
              -ua_parms when using -url, hashref of key => value parameters
                        to pass to LWP::UserAgent->new(). A useful value might
                        be, for example, {timeout => 60 } (ua defaults to 180s)
 Returns : True

=cut

sub _initialize_io {
    my($self, @args) = @_;

    $self->_register_for_cleanup(\&_io_cleanup);

    my ($input, $noclose, $file, $fh, $string,
        $flush, $url, $retries, $ua_parms) =
        $self->_rearrange([qw(INPUT NOCLOSE FILE FH STRING FLUSH URL RETRIES UA_PARMS)],
                          @args);

    my $mode;

    if ($url) {
        $retries ||= 5;

        require LWP::UserAgent;
        my $ua = LWP::UserAgent->new(%$ua_parms);
        my $http_result;
        my ($handle, $tempfile) = $self->tempfile();
        CORE::close($handle);

        for (my $try = 1 ; $try <= $retries ; $try++) {
            $http_result = $ua->get($url, ':content_file' => $tempfile);
            $self->warn("[$try/$retries] tried to fetch $url, but server ".
                        "threw ". $http_result->code . ".  retrying...")
              if !$http_result->is_success;
            last if $http_result->is_success;
        }
        $self->throw("Failed to fetch $url, server threw ".$http_result->code)
          if !$http_result->is_success;

        $file = $tempfile;
        $mode = '>';
    }

    delete $self->{'_readbuffer'};
    delete $self->{'_filehandle'};
    $self->noclose( $noclose) if defined $noclose;
    # determine whether the input is a file(name) or a stream
    if ($input) {
        if (ref(\$input) eq 'SCALAR') {
            # we assume that a scalar is a filename
            if ($file && ($file ne $input)) {
                $self->throw("Input file given twice: '$file' and '$input' disagree");
            }
            $file = $input;
        } elsif (ref($input) &&
            ((ref($input) eq 'GLOB') || $input->isa('IO::Handle'))) {
            # input is a stream
            $fh = $input;
        } else {
            # let's be strict for now
            $self->throw("Unable to determine type of input $input: ".
                         "not string and not GLOB");
        }
    }

    if (defined($file) && defined($fh)) {
        $self->throw("Providing both a file and a filehandle for reading - ".
                     "only one please!");
    }

    if ($string) {
        if (defined($file) || defined($fh)) {
            $self->throw("File or filehandle provided with -string, ".
                         "please unset if you are using -string as a file");
        }
        open $fh, '<', \$string or $self->throw("Could not read string: $!");
    }

    if (defined($file) && ($file ne '')) {
        $self->file($file);
        ($mode, $file) = $self->cleanfile;
        $mode ||= '<';
        my $action = ($mode =~ m/>/) ? 'write' : 'read';
        $fh = Symbol::gensym();
        open $fh, $mode, $file or $self->throw("Could not $action file '$file': $!");
    }

    if (defined $fh) {
        # check filehandle to ensure it's one of:
        # a GLOB reference, as in: open(my $fh, "myfile");
        # an IO::Handle or IO::String object
        # the UNIVERSAL::can added to fix Bug2863
        unless (   ( ref $fh and ( ref $fh eq 'GLOB' ) )
                or ( ref $fh and ( UNIVERSAL::can( $fh, 'can' ) )
                             and (   $fh->isa('IO::Handle')
                                  or $fh->isa('IO::String') ) )
               ) {
            $self->throw("Object $fh does not appear to be a file handle");
        }
        if ($HAS_EOL) {
            binmode $fh, ':raw:eol(LF-Native)';
        }
        $self->_fh($fh); # if $fh not provided, defaults to STDIN and STDOUT
    }

    $self->_flush_on_write(defined $flush ? $flush : 1);

    return 1;
}


=head2 _fh

 Title   : _fh
 Usage   : $io->_fh($newval);
 Function: Get or set the file handle for the stream encapsulated.
 Args    : Optional filehandle to use
 Returns : Filehandle for the stream

=cut

sub _fh {
    my ($self, $value) = @_;
    if ( defined $value) {
        $self->{'_filehandle'} = $value;
    }
    return $self->{'_filehandle'};
}


=head2 mode

 Title   : mode
 Usage   : $io->mode();
           $io->mode(-force => 1);
 Function: Determine if the object was opened for reading or writing
 Args    : -force: Boolean. Once mode() has been called, the mode is cached for
                   further calls to mode(). Use this argument to override this
                   behavior and re-check the object's mode.
 Returns : Mode of the object:
            'r'  for readable
            'w'  for writable
            'rw' for readable and writable
            '?'  if mode could not be determined (e.g. for a -url)

=cut

sub mode {
    my ($self, %arg) = @_;

    # Method 1: IO::Handle::fdopen
    #    my $iotest = new IO::Handle;
    #    $iotest->fdopen( dup(fileno($fh)) , 'r' );
    #    if ($iotest->error == 0) { ... }
    # It did not actually seem to work under any platform, since there would no
    # error if the filehandle had been opened writable only. It could not be
    # hacked around when dealing with unseekable (piped) filehandles.

    # Method 2: readline, a.k.a. the <> operator
    #    no warnings "io";
    #    my $line = <$fh>;
    #    if (defined $line) {
    #       $self->{'_mode'} = 'r';
    #    ...
    # It did not work well either because <> returns undef, i.e. querying the
    # mode() after having read an entire file returned 'w'.

    if ( $arg{-force} || not exists $self->{'_mode'} ) {
        # Determine stream mode
        my $mode;
        my $fh = $self->_fh;
        if (defined $fh) {
            # Determine read/write status of filehandle
            no warnings 'io';
            if ( defined( read $fh, my $content, 0 ) ) {
                # Successfully read 0 bytes
                $mode = 'r'
            }
            if ( defined( syswrite $fh, '') ) {
                # Successfully wrote 0 bytes
                $mode ||= '';
                $mode  .= 'w';
            }
        } else {
           # Stream does not have a filehandle... cannot determine mode
           $mode = '?';
        }
        # Save mode for future use
        $self->{'_mode'} = $mode;
    }
    return $self->{'_mode'};
}


=head2 file

 Title   : file
 Usage   : $io->file('>'.$file);
           my $file = $io->file;
 Function: Get or set the name of the file to read or write.
 Args    : Optional file name (including its mode, e.g. '<' for reading or '>'
           for writing)
 Returns : A string representing the filename and its mode.

=cut

sub file {
    my ($self, $value) = @_;
    if ( defined $value) {
        $self->{'_file'} = $value;
    }
    return $self->{'_file'};
}


=head2 cleanfile

 Title   : cleanfile
 Usage   : my ($mode, $file) = $io->cleanfile;
 Function: Get the name of the file to read or write, stripped of its mode
           ('>', '<', '+>', '>>', etc).
 Args    : None
 Returns : In array context, an array of the mode and the clean filename.

=cut

sub cleanfile {
    my ($self) = @_;
    return ($self->{'_file'} =~ m/^ (\+?[><]{1,2})?\s*(.*) $/x);
}


=head2 format

 Title   : format
 Usage   : $io->format($newval)
 Function: Get the format of a Bio::Root::IO sequence file or filehandle. Every
           object inheriting Bio::Root::IO is guaranteed to have a format.
 Args    : None
 Returns : Format of the file or filehandle, e.g. fasta, fastq, genbank, embl.

=cut

sub format {
    my ($self) = @_;
    my $format = (split '::', ref($self))[-1];
    return $format;
}


=head2 variant

 Title   : format
 Usage   : $io->format($newval)
 Function: Get the variant of a Bio::Root::IO sequence file or filehandle.
           The format variant depends on the specific format used. Note that
           not all formats have variants. Also, the Bio::Root::IO-implementing
           modules that require access to variants need to define a global hash
           that has the allowed variants as its keys.
 Args    : None
 Returns : Variant of the file or filehandle, e.g. sanger, solexa or illumina for
           the fastq format, or undef for formats that do not have variants.

=cut

sub variant {
    my ($self, $variant) = @_;
    if (defined $variant) {
        $variant = lc $variant;
        my $var_name = '%'.ref($self).'::variant';
        my %ok_variants = eval $var_name; # e.g. %Bio::Assembly::IO::ace::variant
        if (scalar keys %ok_variants == 0) {
            $self->throw("Could not validate variant because global variant ".
                         "$var_name was not set or was empty\n");
        }
        if (not exists $ok_variants{$variant}) {
            $self->throw("$variant is not a valid variant of the " .
                         $self->format . ' format');
        }
        $self->{variant} = $variant;
    }
    return $self->{variant};
}


=head2 _print

 Title   : _print
 Usage   : $io->_print(@lines)
 Function: Print lines of text to the IO stream object.
 Args    : List of strings to print
 Returns : True on success, undef on failure

=cut

sub _print {
    my $self = shift;
    my $fh = $self->_fh() || \*STDOUT;
    my $ret = print $fh @_;
    return $ret;
}


=head2 _insert

 Title   : _insert
 Usage   : $io->_insert($string,1)
 Function: Insert some text in a file at the given line number (1-based).
 Args    : * string to write in file
           * line number to insert the string at
 Returns : True

=cut

sub _insert {
    my ($self, $string, $line_num) = @_;
    # Line number check
    if ($line_num < 1) {
        $self->throw("Could not insert text at line $line_num: the minimum ".
                     "line number possible is 1.");
    }
    # File check
    my ($mode, $file) = $self->cleanfile;
    if (not defined $file) {
        $self->throw('Could not insert a line: IO object was initialized with '.
                     'something else than a file.');
    }
    # Everything that needs to be written is written before we read it
    $self->flush;

    # Edit the file line by line (no slurping)
    $self->close;
    my $temp_file;
    my $number = 0;
    while (-e "$file.$number.temp") {
        $number++;
    }
    $temp_file = "$file.$number.temp";
    copy($file, $temp_file);
    open my $fh1, '<', $temp_file or $self->throw("Could not read temporary file '$temp_file': $!");
    open my $fh2, '>', $file      or $self->throw("Could not write file '$file': $!");
    while (my $line = <$fh1>) {
        if ($. == $line_num) { # right line for new data
            print $fh2 $string . $line;
        }
        else {
            print $fh2 $line;
        }
    }
    CORE::close $fh1;
    CORE::close $fh2;
    unlink $temp_file or $self->throw("Could not delete temporary file '$temp_file': $!");

    # Line number check (again)
    if ( $. > 0 && $line_num > $. ) {
        $self->throw("Could not insert text at line $line_num: there are only ".
                     "$. lines in file '$file'");
    }
    # Re-open the file in append mode to be ready to add text at the end of it
    # when the next _print() statement comes
    open my $new_fh, '>>', $file or $self->throw("Could not append to file '$file': $!");
    $self->_fh($new_fh);
    # If file is empty and we're inserting at line 1, simply append text to file
    if ( $. == 0 && $line_num == 1 ) {
        $self->_print($string);
    }
    return 1;
}


=head2 _readline

 Title   : _readline
 Usage   : local $Bio::Root::IO::HAS_EOL = 1;
           my $io = Bio::Root::IO->new(-file => 'data.txt');
           my $line = $io->_readline();
           $io->close;
 Function: Read a line of input and normalize all end of line characters.

           End of line characters are typically "\n" on Linux platforms, "\r\n"
           on Windows and "\r" on older Mac OS. By default, the _readline()
           method uses the value of $/, Perl's input record separator, to
           detect the end of each line. This means that you will not get the
           expected lines if your input has Mac-formatted end of line characters.
           Also, note that the current implementation does not handle pushed
           back input correctly unless the pushed back input ends with the
           value of $/. For each line parsed, its line ending, e.g. "\r\n" is
           converted to "\n", unless you provide the -raw argument.

           Altogether it is easier to let the PerlIO::eol module automatically
           detect the proper end of line character and normalize it to "\n". Do
           so by setting $Bio::Root::IO::HAS_EOL to 1.

 Args    : -raw : Avoid converting end of line characters to "\n" This option
                  has no effect when using $Bio::Root::IO::HAS_EOL = 1.
 Returns : Line of input, or undef when there is nothing to read anymore

=cut

sub _readline {
    my ($self, %param) = @_;
    my $fh = $self->_fh or return;
    my $line;

    # if the buffer been filled by _pushback then return the buffer
    # contents, rather than read from the filehandle
    if( @{$self->{'_readbuffer'} || [] } ) {
        $line = shift @{$self->{'_readbuffer'}};
    } else {
        $line = <$fh>;
    }

    # Note: In Windows the "-raw" parameter has no effect, because Perl already discards
    # the '\r' from the line when reading in text mode from the filehandle
    # ($line = <$fh>), and put it back automatically when printing
    if( !$HAS_EOL && !$param{-raw} && (defined $line) ) {
        # don't strip line endings if -raw or $HAS_EOL is specified
        $line =~ s/\015\012/\012/g;         # Change all CR/LF pairs to LF
        $line =~ tr/\015/\n/ unless $ONMAC; # Change all single CRs to NEWLINE
    }
    return $line;
}


=head2 _pushback

 Title   : _pushback
 Usage   : $io->_pushback($newvalue)
 Function: Puts a line previously read with _readline back into a buffer.
           buffer can hold as many lines as system memory permits.

           Note that this is only supported for pushing back data ending with
           the current, localized value of $/. Using this method to push
           modified data back onto the buffer stack is not supported; see bug
           843.

 Args    : newvalue
 Returns : True

=cut

# fix for bug 843, this reveals some unsupported behavior

#sub _pushback {
#    my ($self, $value) = @_;
#    if (index($value, $/) >= 0) {
#        push @{$self->{'_readbuffer'}}, $value;
#    } else {
#        $self->throw("Pushing modifed data back not supported: $value");
#    }
#}

sub _pushback {
    my ($self, $value) = @_;
    return unless $value;
    unshift @{$self->{'_readbuffer'}}, $value;
    return 1;
}


=head2 close

 Title   : close
 Usage   : $io->close()
 Function: Closes the file handle associated with this IO instance,
           excepted if -noclose was specified.
 Args    : None
 Returns : True

=cut

sub close {
    my ($self) = @_;

    # do not close if we explicitly asked not to
    return if $self->noclose;

    if( defined( my $fh = $self->{'_filehandle'} )) {
        $self->flush;
        return if ref $fh eq 'GLOB' && (
            \*STDOUT == $fh || \*STDERR == $fh || \*STDIN  == $fh
        );

        # don't close IO::Strings
        CORE::close $fh unless ref $fh && $fh->isa('IO::String');
    }
    $self->{'_filehandle'} = undef;
    delete $self->{'_readbuffer'};
    return 1;
}


=head2 flush

 Title   : flush
 Usage   : $io->flush()
 Function: Flushes the filehandle
 Args    : None
 Returns : True

=cut

sub flush {
    my ($self) = shift;

    if( !defined $self->{'_filehandle'} ) {
        $self->throw("Flush failed: no filehandle was active");
    }

    if( ref($self->{'_filehandle'}) =~ /GLOB/ ) {
        my $oldh = select($self->{'_filehandle'});
        $| = 1;
        select($oldh);
    } else {
        $self->{'_filehandle'}->flush();
    }
    return 1;
}


=head2 noclose

 Title   : noclose
 Usage   : $io->noclose($newval)
 Function: Get or set the NOCLOSE flag - setting this to true will prevent a
           filehandle from being closed when an object is cleaned up or
           explicitly closed.
 Args    : Optional new value (a scalar or undef)
 Returns : Value of noclose (a scalar)

=cut

sub noclose {
    my $self = shift;
    return $self->{'_noclose'} = shift if @_;
    return $self->{'_noclose'};
}


=head2 _io_cleanup

=cut

sub _io_cleanup {
    my ($self) = @_;
    $self->close();
    my $v = $self->verbose;

    # we are planning to cleanup temp files no matter what
    if (    exists($self->{'_rootio_tempfiles'})
        and ref($self->{'_rootio_tempfiles'}) =~ /array/i
        and not $self->save_tempfiles
        ) {
        if( $v > 0 ) {
            warn( "going to remove files ",
                  join(",",  @{$self->{'_rootio_tempfiles'}}),
                  "\n");
        }
        unlink  (@{$self->{'_rootio_tempfiles'}} );
    }
    # cleanup if we are not using File::Temp
    if (    $self->{'_cleanuptempdir'}
        and exists($self->{'_rootio_tempdirs'})
        and ref($self->{'_rootio_tempdirs'}) =~ /array/i
        and not $self->save_tempfiles
        ) {
        if( $v > 0 ) {
            warn( "going to remove dirs ",
                  join(",",  @{$self->{'_rootio_tempdirs'}}),
                  "\n");
        }
        $self->rmtree( $self->{'_rootio_tempdirs'});
    }
}


=head2 exists_exe

 Title   : exists_exe
 Usage   : $exists = $io->exists_exe('clustalw');
           $exists = Bio::Root::IO->exists_exe('clustalw')
           $exists = Bio::Root::IO::exists_exe('clustalw')
 Function: Determines whether the given executable exists either as file
           or within the path environment. The latter requires File::Spec
           to be installed.
           On Win32-based system, .exe is automatically appended to the program
           name unless the program name already ends in .exe.
 Args    : Name of the executable
 Returns : 1 if the given program is callable as an executable, and 0 otherwise

=cut

sub exists_exe {
    my ($self, $exe) = @_;
    $self->throw("Must pass a defined value to exists_exe") unless defined $exe;
    $exe = $self if (!(ref($self) || $exe));
    $exe .= '.exe' if(($^O =~ /mswin/i) && ($exe !~ /\.(exe|com|bat|cmd)$/i));
    return $exe if ( -f $exe && -x $exe ); # full path and exists

    # Ewan's comment. I don't think we need this. People should not be
    # asking for a program with a pathseparator starting it
    # $exe =~ s/^$PATHSEP//;

    # Not a full path, or does not exist. Let's see whether it's in the path.
    if($FILESPECLOADED) {
        for my $dir (File::Spec->path()) {
            my $f = Bio::Root::IO->catfile($dir, $exe);
            return $f if( -f $f && -x $f );
        }
    }
    return 0;
}


=head2 tempfile

 Title   : tempfile
 Usage   : my ($handle,$tempfile) = $io->tempfile();
 Function: Create a temporary filename and a handle opened for reading and
           writing.
           Caveats: If you do not have File::Temp on your system you should
           avoid specifying TEMPLATE and SUFFIX.
 Args    : Named parameters compatible with File::Temp: DIR (defaults to
           $Bio::Root::IO::TEMPDIR), TEMPLATE, SUFFIX.
 Returns : A 2-element array, consisting of temporary handle and temporary
           file name.

=cut

sub tempfile {
    my ($self, @args) = @_;
    my ($tfh, $file);
    my %params = @args;

    # map between naming with and without dash
    for my $key (keys(%params)) {
        if( $key =~ /^-/  ) {
            my $v = $params{$key};
            delete $params{$key};
            $params{uc(substr($key,1))} = $v;
        } else {
            # this is to upper case
            my $v = $params{$key};
            delete $params{$key};
            $params{uc($key)} = $v;
        }
    }
    $params{'DIR'} = $TEMPDIR if(! exists($params{'DIR'}));
    unless (exists $params{'UNLINK'} &&
            defined $params{'UNLINK'} &&
            ! $params{'UNLINK'} ) {
        $params{'UNLINK'} = 1;
    } else {
        $params{'UNLINK'} = 0;
    }

    if($FILETEMPLOADED) {
        if(exists($params{'TEMPLATE'})) {
            my $template = $params{'TEMPLATE'};
            delete $params{'TEMPLATE'};
            ($tfh, $file) = File::Temp::tempfile($template, %params);
        } else {
            ($tfh, $file) = File::Temp::tempfile(%params);
        }
    } else {
        my $dir = $params{'DIR'};
        $file = $self->catfile(
            $dir,
            (exists($params{'TEMPLATE'}) ?
             $params{'TEMPLATE'} :
             sprintf( "%s.%s.%s", $ENV{USER} || 'unknown', $$, $TEMPCOUNTER++))
        );

        # sneakiness for getting around long filenames on Win32?
        if( $HAS_WIN32 ) {
            $file = Win32::GetShortPathName($file);
        }

        # Try to make sure this will be marked close-on-exec
        # XXX: Win32 doesn't respect this, nor the proper fcntl,
        #      but may have O_NOINHERIT. This may or may not be in Fcntl.
        local $^F = 2;
        # Store callers umask
        my $umask = umask();
        # Set a known umaskr
        umask(066);
        # Attempt to open the file
        if ( sysopen($tfh, $file, $OPENFLAGS, 0600) ) {
            # Reset umask
            umask($umask);
        } else {
            $self->throw("Could not write temporary file '$file': $!");
        }
    }

    if(  $params{'UNLINK'} ) {
        push @{$self->{'_rootio_tempfiles'}}, $file;
    }

    return wantarray ? ($tfh,$file) : $tfh;
}


=head2  tempdir

 Title   : tempdir
 Usage   : my ($tempdir) = $io->tempdir(CLEANUP=>1);
 Function: Creates and returns the name of a new temporary directory.

           Note that you should not use this function for obtaining "the"
           temp directory. Use $Bio::Root::IO::TEMPDIR for that. Calling this
           method will in fact create a new directory.

 Args    : args - ( key CLEANUP ) indicates whether or not to cleanup
           dir on object destruction, other keys as specified by File::Temp
 Returns : The name of a new temporary directory.

=cut

sub tempdir {
    my ($self, @args) = @_;
    if ($FILETEMPLOADED && File::Temp->can('tempdir')) {
        return File::Temp::tempdir(@args);
    }

    # we have to do this ourselves, not good
    # we are planning to cleanup temp files no matter what
    my %params = @args;
    print "cleanup is " . $params{CLEANUP} . "\n";
    $self->{'_cleanuptempdir'} = ( defined $params{CLEANUP} &&
                                   $params{CLEANUP} == 1);
    my $tdir = $self->catfile( $TEMPDIR,
                               sprintf("dir_%s-%s-%s",
                                       $ENV{USER} || 'unknown',
                                       $$,
                                       $TEMPCOUNTER++));
    mkdir($tdir, 0755);
    push @{$self->{'_rootio_tempdirs'}}, $tdir;
    return $tdir;
}


=head2 catfile

 Title   : catfile
 Usage   : $path = Bio::Root::IO->catfile(@dirs, $filename);
 Function: Constructs a full pathname in a cross-platform safe way.

           If File::Spec exists on your system, this routine will merely
           delegate to it. Otherwise it tries to make a good guess.

           You should use this method whenever you construct a path name
           from directory and filename. Otherwise you risk cross-platform
           compatibility of your code.

           You can call this method both as a class and an instance method.

 Args    : components of the pathname (directories and filename, NOT an
           extension)
 Returns : a string

=cut

sub catfile {
    my ($self, @args) = @_;

    return File::Spec->catfile(@args) if $FILESPECLOADED;
    # this is clumsy and not very appealing, but how do we specify the
    # root directory?
    if($args[0] eq '/') {
        $args[0] = $ROOTDIR;
    }
    return join($PATHSEP, @args);
}


=head2 rmtree

 Title   : rmtree
 Usage   : Bio::Root::IO->rmtree($dirname );
 Function: Remove a full directory tree

           If File::Path exists on your system, this routine will merely
           delegate to it. Otherwise it runs a local version of that code.

           You should use this method to remove directories which contain
           files.

           You can call this method both as a class and an instance method.

 Args    : roots - rootdir to delete or reference to list of dirs

           verbose - a boolean value, which if TRUE will cause
                     C<rmtree> to print a message each time it
                     examines a file, giving the name of the file, and
                     indicating whether it's using C<rmdir> or
                     C<unlink> to remove it, or that it's skipping it.
                     (defaults to FALSE)

           safe - a boolean value, which if TRUE will cause C<rmtree>
                  to skip any files to which you do not have delete
                  access (if running under VMS) or write access (if
                  running under another OS).  This will change in the
                  future when a criterion for 'delete permission'
                  under OSs other than VMS is settled.  (defaults to
                  FALSE)
 Returns : number of files successfully deleted

=cut

# taken straight from File::Path VERSION = "1.0403"
sub rmtree {
    my ($self, $roots, $verbose, $safe) = @_;
    if ( $FILEPATHLOADED ) {
        return File::Path::rmtree ($roots, $verbose, $safe);
    }

    my $force_writable = ($^O eq 'os2' || $^O eq 'dos' || $^O eq 'MSWin32' ||
                          $^O eq 'amigaos' || $^O eq 'cygwin');
    my $Is_VMS = $^O eq 'VMS';

    my @files;
    my $count = 0;
    $verbose ||= 0;
    $safe    ||= 0;
    if ( defined($roots) && length($roots) ) {
        $roots = [$roots] unless ref $roots;
    } else {
        $self->warn("No root path(s) specified\n");
        return 0;
    }

    my $root;
    for $root (@{$roots}) {
        $root =~ s#/\z##;
        (undef, undef, my $rp) = lstat $root or next;
        $rp &= 07777;   # don't forget setuid, setgid, sticky bits
        if ( -d _ ) {
            # notabene: 0777 is for making readable in the first place,
            # it's also intended to change it to writable in case we have
            # to recurse in which case we are better than rm -rf for
            # subtrees with strange permissions
            chmod(0777, ($Is_VMS ? VMS::Filespec::fileify($root) : $root))
              or $self->warn("Could not make directory '$root' read+writable: $!")
            unless $safe;
            if (opendir DIR, $root){
                @files = readdir DIR;
                closedir DIR;
            } else {
                $self->warn("Could not read directory '$root': $!");
                @files = ();
            }

            # Deleting large numbers of files from VMS Files-11 filesystems
            # is faster if done in reverse ASCIIbetical order
            @files = reverse @files if $Is_VMS;
            ($root = VMS::Filespec::unixify($root)) =~ s#\.dir\z## if $Is_VMS;
            @files = map("$root/$_", grep $_!~/^\.{1,2}\z/s,@files);
            $count += $self->rmtree([@files],$verbose,$safe);
            if ($safe &&
              ($Is_VMS ? !&VMS::Filespec::candelete($root) : !-w $root)) {
                print "skipped '$root'\n" if $verbose;
                next;
            }
            chmod 0777, $root
              or $self->warn("Could not make directory '$root' writable: $!")
              if $force_writable;
            print "rmdir '$root'\n" if $verbose;
            if (rmdir $root) {
                ++$count;
            }
            else {
                $self->warn("Could not remove directory '$root': $!");
                chmod($rp, ($Is_VMS ? VMS::Filespec::fileify($root) : $root))
                  or $self->warn("and can't restore permissions to "
                                 . sprintf("0%o",$rp) . "\n");
            }
        }
        else {
            if (     $safe
                and ($Is_VMS ? !&VMS::Filespec::candelete($root)
                             : !(-l $root || -w $root))
                ) {
                print "skipped '$root'\n" if $verbose;
                next;
            }
            chmod 0666, $root
              or $self->warn( "Could not make file '$root' writable: $!")
              if $force_writable;
            warn "unlink '$root'\n" if $verbose;
            # delete all versions under VMS
            for (;;) {
                unless (unlink $root) {
                    $self->warn("Could not unlink file '$root': $!");
                    if ($force_writable) {
                        chmod $rp, $root
                          or $self->warn("and can't restore permissions to "
                                         . sprintf("0%o",$rp) . "\n");
                    }
                    last;
                }
                ++$count;
                last unless $Is_VMS && lstat $root;
            }
        }
    }

    return $count;
}


=head2 _flush_on_write

 Title   : _flush_on_write
 Usage   : $io->_flush_on_write($newval)
 Function: Boolean flag to indicate whether to flush
           the filehandle on writing when the end of
           a component is finished (Sequences, Alignments, etc)
 Args    : Optional new value
 Returns : Value of _flush_on_write

=cut

sub _flush_on_write {
    my ($self, $value) = @_;
    if (defined $value) {
        $self->{'_flush_on_write'} = $value;
    }
    return $self->{'_flush_on_write'};
}


=head2 save_tempfiles

 Title   : save_tempfiles
 Usage   : $io->save_tempfiles(1)
 Function: Boolean flag to indicate whether to retain tempfiles/tempdir
 Args    : Value evaluating to TRUE or FALSE
 Returns : Boolean value : 1 = save tempfiles/tempdirs, 0 = remove (default)

=cut

sub save_tempfiles {
    my $self = shift;
    if (@_) {
        my $value = shift;
        $self->{save_tempfiles} = $value ? 1 : 0;
    }
    return $self->{save_tempfiles} || 0;
}


1;