/usr/share/pyshared/tifffile.py is in python-tifffile 20131103-1.
This file is owned by root:root, with mode 0o644.
The actual contents of the file can be viewed below.
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96 97 98 99 100 101 102 103 104 105 106 107 108 109 110 111 112 113 114 115 116 117 118 119 120 121 122 123 124 125 126 127 128 129 130 131 132 133 134 135 136 137 138 139 140 141 142 143 144 145 146 147 148 149 150 151 152 153 154 155 156 157 158 159 160 161 162 163 164 165 166 167 168 169 170 171 172 173 174 175 176 177 178 179 180 181 182 183 184 185 186 187 188 189 190 191 192 193 194 195 196 197 198 199 200 201 202 203 204 205 206 207 208 209 210 211 212 213 214 215 216 217 218 219 220 221 222 223 224 225 226 227 228 229 230 231 232 233 234 235 236 237 238 239 240 241 242 243 244 245 246 247 248 249 250 251 252 253 254 255 256 257 258 259 260 261 262 263 264 265 266 267 268 269 270 271 272 273 274 275 276 277 278 279 280 281 282 283 284 285 286 287 288 289 290 291 292 293 294 295 296 297 298 299 300 301 302 303 304 305 306 307 308 309 310 311 312 313 314 315 316 317 318 319 320 321 322 323 324 325 326 327 328 329 330 331 332 333 334 335 336 337 338 339 340 341 342 343 344 345 346 347 348 349 350 351 352 353 354 355 356 357 358 359 360 361 362 363 364 365 366 367 368 369 370 371 372 373 374 375 376 377 378 379 380 381 382 383 384 385 386 387 388 389 390 391 392 393 394 395 396 397 398 399 400 401 402 403 404 405 406 407 408 409 410 411 412 413 414 415 416 417 418 419 420 421 422 423 424 425 426 427 428 429 430 431 432 433 434 435 436 437 438 439 440 441 442 443 444 445 446 447 448 449 450 451 452 453 454 455 456 457 458 459 460 461 462 463 464 465 466 467 468 469 470 471 472 473 474 475 476 477 478 479 480 481 482 483 484 485 486 487 488 489 490 491 492 493 494 495 496 497 498 499 500 501 502 503 504 505 506 507 508 509 510 511 512 513 514 515 516 517 518 519 520 521 522 523 524 525 526 527 528 529 530 531 532 533 534 535 536 537 538 539 540 541 542 543 544 545 546 547 548 549 550 551 552 553 554 555 556 557 558 559 560 561 562 563 564 565 566 567 568 569 570 571 572 573 574 575 576 577 578 579 580 581 582 583 584 585 586 587 588 589 590 591 592 593 594 595 596 597 598 599 600 601 602 603 604 605 606 607 608 609 610 611 612 613 614 615 616 617 618 619 620 621 622 623 624 625 626 627 628 629 630 631 632 633 634 635 636 637 638 639 640 641 642 643 644 645 646 647 648 649 650 651 652 653 654 655 656 657 658 659 660 661 662 663 664 665 666 667 668 669 670 671 672 673 674 675 676 677 678 679 680 681 682 683 684 685 686 687 688 689 690 691 692 693 694 695 696 697 698 699 700 701 702 703 704 705 706 707 708 709 710 711 712 713 714 715 716 717 718 719 720 721 722 723 724 725 726 727 728 729 730 731 732 733 734 735 736 737 738 739 740 741 742 743 744 745 746 747 748 749 750 751 752 753 754 755 756 757 758 759 760 761 762 763 764 765 766 767 768 769 770 771 772 773 774 775 776 777 778 779 780 781 782 783 784 785 786 787 788 789 790 791 792 793 794 795 796 797 798 799 800 801 802 803 804 805 806 807 808 809 810 811 812 813 814 815 816 817 818 819 820 821 822 823 824 825 826 827 828 829 830 831 832 833 834 835 836 837 838 839 840 841 842 843 844 845 846 847 848 849 850 851 852 853 854 855 856 857 858 859 860 861 862 863 864 865 866 867 868 869 870 871 872 873 874 875 876 877 878 879 880 881 882 883 884 885 886 887 888 889 890 891 892 893 894 895 896 897 898 899 900 901 902 903 904 905 906 907 908 909 910 911 912 913 914 915 916 917 918 919 920 921 922 923 924 925 926 927 928 929 930 931 932 933 934 935 936 937 938 939 940 941 942 943 944 945 946 947 948 949 950 951 952 953 954 955 956 957 958 959 960 961 962 963 964 965 966 967 968 969 970 971 972 973 974 975 976 977 978 979 980 981 982 983 984 985 986 987 988 989 990 991 992 993 994 995 996 997 998 999 1000 1001 1002 1003 1004 1005 1006 1007 1008 1009 1010 1011 1012 1013 1014 1015 1016 1017 1018 1019 1020 1021 1022 1023 1024 1025 1026 1027 1028 1029 1030 1031 1032 1033 1034 1035 1036 1037 1038 1039 1040 1041 1042 1043 1044 1045 1046 1047 1048 1049 1050 1051 1052 1053 1054 1055 1056 1057 1058 1059 1060 1061 1062 1063 1064 1065 1066 1067 1068 1069 1070 1071 1072 1073 1074 1075 1076 1077 1078 1079 1080 1081 1082 1083 1084 1085 1086 1087 1088 1089 1090 1091 1092 1093 1094 1095 1096 1097 1098 1099 1100 1101 1102 1103 1104 1105 1106 1107 1108 1109 1110 1111 1112 1113 1114 1115 1116 1117 1118 1119 1120 1121 1122 1123 1124 1125 1126 1127 1128 1129 1130 1131 1132 1133 1134 1135 1136 1137 1138 1139 1140 1141 1142 1143 1144 1145 1146 1147 1148 1149 1150 1151 1152 1153 1154 1155 1156 1157 1158 1159 1160 1161 1162 1163 1164 1165 1166 1167 1168 1169 1170 1171 1172 1173 1174 1175 1176 1177 1178 1179 1180 1181 1182 1183 1184 1185 1186 1187 1188 1189 1190 1191 1192 1193 1194 1195 1196 1197 1198 1199 1200 1201 1202 1203 1204 1205 1206 1207 1208 1209 1210 1211 1212 1213 1214 1215 1216 1217 1218 1219 1220 1221 1222 1223 1224 1225 1226 1227 1228 1229 1230 1231 1232 1233 1234 1235 1236 1237 1238 1239 1240 1241 1242 1243 1244 1245 1246 1247 1248 1249 1250 1251 1252 1253 1254 1255 1256 1257 1258 1259 1260 1261 1262 1263 1264 1265 1266 1267 1268 1269 1270 1271 1272 1273 1274 1275 1276 1277 1278 1279 1280 1281 1282 1283 1284 1285 1286 1287 1288 1289 1290 1291 1292 1293 1294 1295 1296 1297 1298 1299 1300 1301 1302 1303 1304 1305 1306 1307 1308 1309 1310 1311 1312 1313 1314 1315 1316 1317 1318 1319 1320 1321 1322 1323 1324 1325 1326 1327 1328 1329 1330 1331 1332 1333 1334 1335 1336 1337 1338 1339 1340 1341 1342 1343 1344 1345 1346 1347 1348 1349 1350 1351 1352 1353 1354 1355 1356 1357 1358 1359 1360 1361 1362 1363 1364 1365 1366 1367 1368 1369 1370 1371 1372 1373 1374 1375 1376 1377 1378 1379 1380 1381 1382 1383 1384 1385 1386 1387 1388 1389 1390 1391 1392 1393 1394 1395 1396 1397 1398 1399 1400 1401 1402 1403 1404 1405 1406 1407 1408 1409 1410 1411 1412 1413 1414 1415 1416 1417 1418 1419 1420 1421 1422 1423 1424 1425 1426 1427 1428 1429 1430 1431 1432 1433 1434 1435 1436 1437 1438 1439 1440 1441 1442 1443 1444 1445 1446 1447 1448 1449 1450 1451 1452 1453 1454 1455 1456 1457 1458 1459 1460 1461 1462 1463 1464 1465 1466 1467 1468 1469 1470 1471 1472 1473 1474 1475 1476 1477 1478 1479 1480 1481 1482 1483 1484 1485 1486 1487 1488 1489 1490 1491 1492 1493 1494 1495 1496 1497 1498 1499 1500 1501 1502 1503 1504 1505 1506 1507 1508 1509 1510 1511 1512 1513 1514 1515 1516 1517 1518 1519 1520 1521 1522 1523 1524 1525 1526 1527 1528 1529 1530 1531 1532 1533 1534 1535 1536 1537 1538 1539 1540 1541 1542 1543 1544 1545 1546 1547 1548 1549 1550 1551 1552 1553 1554 1555 1556 1557 1558 1559 1560 1561 1562 1563 1564 1565 1566 1567 1568 1569 1570 1571 1572 1573 1574 1575 1576 1577 1578 1579 1580 1581 1582 1583 1584 1585 1586 1587 1588 1589 1590 1591 1592 1593 1594 1595 1596 1597 1598 1599 1600 1601 1602 1603 1604 1605 1606 1607 1608 1609 1610 1611 1612 1613 1614 1615 1616 1617 1618 1619 1620 1621 1622 1623 1624 1625 1626 1627 1628 1629 1630 1631 1632 1633 1634 1635 1636 1637 1638 1639 1640 1641 1642 1643 1644 1645 1646 1647 1648 1649 1650 1651 1652 1653 1654 1655 1656 1657 1658 1659 1660 1661 1662 1663 1664 1665 1666 1667 1668 1669 1670 1671 1672 1673 1674 1675 1676 1677 1678 1679 1680 1681 1682 1683 1684 1685 1686 1687 1688 1689 1690 1691 1692 1693 1694 1695 1696 1697 1698 1699 1700 1701 1702 1703 1704 1705 1706 1707 1708 1709 1710 1711 1712 1713 1714 1715 1716 1717 1718 1719 1720 1721 1722 1723 1724 1725 1726 1727 1728 1729 1730 1731 1732 1733 1734 1735 1736 1737 1738 1739 1740 1741 1742 1743 1744 1745 1746 1747 1748 1749 1750 1751 1752 1753 1754 1755 1756 1757 1758 1759 1760 1761 1762 1763 1764 1765 1766 1767 1768 1769 1770 1771 1772 1773 1774 1775 1776 1777 1778 1779 1780 1781 1782 1783 1784 1785 1786 1787 1788 1789 1790 1791 1792 1793 1794 1795 1796 1797 1798 1799 1800 1801 1802 1803 1804 1805 1806 1807 1808 1809 1810 1811 1812 1813 1814 1815 1816 1817 1818 1819 1820 1821 1822 1823 1824 1825 1826 1827 1828 1829 1830 1831 1832 1833 1834 1835 1836 1837 1838 1839 1840 1841 1842 1843 1844 1845 1846 1847 1848 1849 1850 1851 1852 1853 1854 1855 1856 1857 1858 1859 1860 1861 1862 1863 1864 1865 1866 1867 1868 1869 1870 1871 1872 1873 1874 1875 1876 1877 1878 1879 1880 1881 1882 1883 1884 1885 1886 1887 1888 1889 1890 1891 1892 1893 1894 1895 1896 1897 1898 1899 1900 1901 1902 1903 1904 1905 1906 1907 1908 1909 1910 1911 1912 1913 1914 1915 1916 1917 1918 1919 1920 1921 1922 1923 1924 1925 1926 1927 1928 1929 1930 1931 1932 1933 1934 1935 1936 1937 1938 1939 1940 1941 1942 1943 1944 1945 1946 1947 1948 1949 1950 1951 1952 1953 1954 1955 1956 1957 1958 1959 1960 1961 1962 1963 1964 1965 1966 1967 1968 1969 1970 1971 1972 1973 1974 1975 1976 1977 1978 1979 1980 1981 1982 1983 1984 1985 1986 1987 1988 1989 1990 1991 1992 1993 1994 1995 1996 1997 1998 1999 2000 2001 2002 2003 2004 2005 2006 2007 2008 2009 2010 2011 2012 2013 2014 2015 2016 2017 2018 2019 2020 2021 2022 2023 2024 2025 2026 2027 2028 2029 2030 2031 2032 2033 2034 2035 2036 2037 2038 2039 2040 2041 2042 2043 2044 2045 2046 2047 2048 2049 2050 2051 2052 2053 2054 2055 2056 2057 2058 2059 2060 2061 2062 2063 2064 2065 2066 2067 2068 2069 2070 2071 2072 2073 2074 2075 2076 2077 2078 2079 2080 2081 2082 2083 2084 2085 2086 2087 2088 2089 2090 2091 2092 2093 2094 2095 2096 2097 2098 2099 2100 2101 2102 2103 2104 2105 2106 2107 2108 2109 2110 2111 2112 2113 2114 2115 2116 2117 2118 2119 2120 2121 2122 2123 2124 2125 2126 2127 2128 2129 2130 2131 2132 2133 2134 2135 2136 2137 2138 2139 2140 2141 2142 2143 2144 2145 2146 2147 2148 2149 2150 2151 2152 2153 2154 2155 2156 2157 2158 2159 2160 2161 2162 2163 2164 2165 2166 2167 2168 2169 2170 2171 2172 2173 2174 2175 2176 2177 2178 2179 2180 2181 2182 2183 2184 2185 2186 2187 2188 2189 2190 2191 2192 2193 2194 2195 2196 2197 2198 2199 2200 2201 2202 2203 2204 2205 2206 2207 2208 2209 2210 2211 2212 2213 2214 2215 2216 2217 2218 2219 2220 2221 2222 2223 2224 2225 2226 2227 2228 2229 2230 2231 2232 2233 2234 2235 2236 2237 2238 2239 2240 2241 2242 2243 2244 2245 2246 2247 2248 2249 2250 2251 2252 2253 2254 2255 2256 2257 2258 2259 2260 2261 2262 2263 2264 2265 2266 2267 2268 2269 2270 2271 2272 2273 2274 2275 2276 2277 2278 2279 2280 2281 2282 2283 2284 2285 2286 2287 2288 2289 2290 2291 2292 2293 2294 2295 2296 2297 2298 2299 2300 2301 2302 2303 2304 2305 2306 2307 2308 2309 2310 2311 2312 2313 2314 2315 2316 2317 2318 2319 2320 2321 2322 2323 2324 2325 2326 2327 2328 2329 2330 2331 2332 2333 2334 2335 2336 2337 2338 2339 2340 2341 2342 2343 2344 2345 2346 2347 2348 2349 2350 2351 2352 2353 2354 2355 2356 2357 2358 2359 2360 2361 2362 2363 2364 2365 2366 2367 2368 2369 2370 2371 2372 2373 2374 2375 2376 2377 2378 2379 2380 2381 2382 2383 2384 2385 2386 2387 2388 2389 2390 2391 2392 2393 2394 2395 2396 2397 2398 2399 2400 2401 2402 2403 2404 2405 2406 2407 2408 2409 2410 2411 2412 2413 2414 2415 2416 2417 2418 2419 2420 2421 2422 2423 2424 2425 2426 2427 2428 2429 2430 2431 2432 2433 2434 2435 2436 2437 2438 2439 2440 2441 2442 2443 2444 2445 2446 2447 2448 2449 2450 2451 2452 2453 2454 2455 2456 2457 2458 2459 2460 2461 2462 2463 2464 2465 2466 2467 2468 2469 2470 2471 2472 2473 2474 2475 2476 2477 2478 2479 2480 2481 2482 2483 2484 2485 2486 2487 2488 2489 2490 2491 2492 2493 2494 2495 2496 2497 2498 2499 2500 2501 2502 2503 2504 2505 2506 2507 2508 2509 2510 2511 2512 2513 2514 2515 2516 2517 2518 2519 2520 2521 2522 2523 2524 2525 2526 2527 2528 2529 2530 2531 2532 2533 2534 2535 2536 2537 2538 2539 2540 2541 2542 2543 2544 2545 2546 2547 2548 2549 2550 2551 2552 2553 2554 2555 2556 2557 2558 2559 2560 2561 2562 2563 2564 2565 2566 2567 2568 2569 2570 2571 2572 2573 2574 2575 2576 2577 2578 2579 2580 2581 2582 2583 2584 2585 2586 2587 2588 2589 2590 2591 2592 2593 2594 2595 2596 2597 2598 2599 2600 2601 2602 2603 2604 2605 2606 2607 2608 2609 2610 2611 2612 2613 2614 2615 2616 2617 2618 2619 2620 2621 2622 2623 2624 2625 2626 2627 2628 2629 2630 2631 2632 2633 2634 2635 2636 2637 2638 2639 2640 2641 2642 2643 2644 2645 2646 2647 2648 2649 2650 2651 2652 2653 2654 2655 2656 2657 2658 2659 2660 2661 2662 2663 2664 2665 2666 2667 2668 2669 2670 2671 2672 2673 2674 2675 2676 2677 2678 2679 2680 2681 2682 2683 2684 2685 2686 2687 2688 2689 2690 2691 2692 2693 2694 2695 2696 2697 2698 2699 2700 2701 2702 2703 2704 2705 2706 2707 2708 2709 2710 2711 2712 2713 2714 2715 2716 2717 2718 2719 2720 2721 2722 2723 2724 2725 2726 2727 2728 2729 2730 2731 2732 2733 2734 2735 2736 2737 2738 2739 2740 2741 2742 2743 2744 2745 2746 2747 2748 2749 2750 2751 2752 2753 2754 2755 2756 2757 2758 2759 2760 2761 2762 2763 2764 2765 2766 2767 2768 2769 2770 2771 2772 2773 2774 2775 2776 2777 2778 2779 2780 2781 2782 2783 2784 2785 2786 2787 2788 2789 2790 2791 2792 2793 2794 2795 2796 2797 2798 2799 2800 2801 2802 2803 2804 2805 2806 2807 2808 2809 2810 2811 2812 2813 2814 2815 2816 2817 2818 2819 2820 2821 2822 2823 2824 2825 2826 2827 2828 2829 2830 2831 2832 2833 2834 2835 2836 2837 2838 2839 2840 2841 2842 2843 2844 2845 2846 2847 2848 2849 2850 2851 2852 2853 2854 2855 2856 2857 2858 2859 2860 2861 2862 2863 2864 2865 2866 2867 2868 2869 2870 2871 2872 2873 2874 2875 2876 2877 2878 2879 2880 2881 2882 2883 2884 2885 2886 2887 2888 2889 2890 2891 2892 2893 2894 2895 2896 2897 2898 2899 2900 2901 2902 2903 2904 2905 2906 2907 2908 2909 2910 2911 2912 2913 2914 2915 2916 2917 2918 2919 2920 2921 2922 2923 2924 2925 2926 2927 2928 2929 2930 2931 2932 2933 2934 2935 2936 2937 2938 2939 2940 2941 2942 2943 2944 2945 2946 2947 2948 2949 2950 2951 2952 2953 2954 2955 2956 2957 2958 2959 2960 2961 2962 2963 2964 2965 2966 2967 2968 2969 2970 2971 2972 2973 2974 2975 2976 2977 2978 2979 2980 2981 2982 2983 2984 2985 2986 2987 2988 2989 2990 2991 2992 2993 2994 2995 2996 2997 2998 2999 3000 3001 3002 3003 3004 3005 3006 3007 3008 3009 3010 3011 3012 3013 3014 3015 3016 3017 3018 3019 3020 3021 3022 3023 3024 3025 3026 3027 3028 3029 3030 3031 3032 3033 3034 3035 3036 3037 3038 3039 3040 3041 3042 3043 3044 3045 3046 3047 3048 3049 3050 3051 3052 3053 3054 3055 3056 3057 3058 3059 3060 3061 3062 3063 3064 3065 3066 3067 3068 3069 3070 3071 3072 3073 3074 3075 3076 3077 3078 3079 3080 3081 3082 3083 3084 3085 3086 3087 3088 3089 3090 3091 3092 3093 3094 3095 3096 3097 3098 3099 3100 3101 3102 3103 3104 3105 3106 3107 3108 3109 3110 3111 3112 3113 3114 3115 3116 3117 3118 3119 3120 3121 3122 3123 3124 3125 3126 3127 3128 3129 3130 3131 3132 3133 3134 3135 3136 3137 3138 3139 3140 3141 3142 3143 3144 3145 3146 3147 3148 3149 3150 3151 3152 3153 3154 3155 3156 3157 3158 3159 3160 3161 3162 3163 3164 3165 3166 3167 3168 3169 3170 3171 3172 3173 3174 3175 3176 3177 3178 3179 3180 3181 3182 3183 3184 3185 3186 3187 3188 3189 3190 3191 3192 3193 3194 3195 3196 3197 3198 3199 3200 3201 3202 3203 3204 3205 3206 3207 3208 3209 3210 3211 3212 3213 3214 3215 3216 3217 3218 3219 3220 3221 3222 3223 3224 3225 3226 3227 3228 3229 3230 3231 3232 3233 3234 3235 3236 3237 3238 3239 3240 3241 3242 3243 3244 3245 3246 3247 3248 3249 3250 3251 3252 3253 3254 3255 3256 3257 3258 3259 3260 3261 3262 3263 3264 3265 3266 3267 3268 3269 3270 | #!/usr/bin/env python
# -*- coding: utf-8 -*-
# tifffile.py
# Copyright (c) 2008-2013, Christoph Gohlke
# Copyright (c) 2008-2013, The Regents of the University of California
# Produced at the Laboratory for Fluorescence Dynamics
# All rights reserved.
#
# Redistribution and use in source and binary forms, with or without
# modification, are permitted provided that the following conditions are met:
#
# * Redistributions of source code must retain the above copyright
# notice, this list of conditions and the following disclaimer.
# * Redistributions in binary form must reproduce the above copyright
# notice, this list of conditions and the following disclaimer in the
# documentation and/or other materials provided with the distribution.
# * Neither the name of the copyright holders nor the names of any
# contributors may be used to endorse or promote products derived
# from this software without specific prior written permission.
#
# THIS SOFTWARE IS PROVIDED BY THE COPYRIGHT HOLDERS AND CONTRIBUTORS "AS IS"
# AND ANY EXPRESS OR IMPLIED WARRANTIES, INCLUDING, BUT NOT LIMITED TO, THE
# IMPLIED WARRANTIES OF MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE
# ARE DISCLAIMED. IN NO EVENT SHALL THE COPYRIGHT OWNER OR CONTRIBUTORS BE
# LIABLE FOR ANY DIRECT, INDIRECT, INCIDENTAL, SPECIAL, EXEMPLARY, OR
# CONSEQUENTIAL DAMAGES (INCLUDING, BUT NOT LIMITED TO, PROCUREMENT OF
# SUBSTITUTE GOODS OR SERVICES; LOSS OF USE, DATA, OR PROFITS; OR BUSINESS
# INTERRUPTION) HOWEVER CAUSED AND ON ANY THEORY OF LIABILITY, WHETHER IN
# CONTRACT, STRICT LIABILITY, OR TORT (INCLUDING NEGLIGENCE OR OTHERWISE)
# ARISING IN ANY WAY OUT OF THE USE OF THIS SOFTWARE, EVEN IF ADVISED OF THE
# POSSIBILITY OF SUCH DAMAGE.
"""Read and write image data from and to TIFF files.
Image and meta-data can be read from TIFF, BigTIFF, OME-TIFF, STK, LSM, NIH,
ImageJ, MicroManager, FluoView, SEQ and GEL files.
Only a subset of the TIFF specification is supported, mainly uncompressed
and losslessly compressed 2**(0 to 6) bit integer, 16, 32 and 64-bit float,
grayscale and RGB(A) images, which are commonly used in bio-scientific imaging.
Specifically, reading JPEG/CCITT compressed image data or EXIF/IPTC/GPS/XMP
meta-data is not implemented. Only primary info records are read for STK,
FluoView, MicroManager, and NIH image formats.
TIFF, the Tagged Image File Format, is under the control of Adobe Systems.
BigTIFF allows for files greater than 4 GB. STK, LSM, FluoView, SEQ, GEL,
and OME-TIFF, are custom extensions defined by MetaMorph, Carl Zeiss
MicroImaging, Olympus, Media Cybernetics, Molecular Dynamics, and the Open
Microscopy Environment consortium respectively.
For command line usage run ``python tifffile.py --help``
:Author:
`Christoph Gohlke <http://www.lfd.uci.edu/~gohlke/>`_
:Organization:
Laboratory for Fluorescence Dynamics, University of California, Irvine
:Version: 2013.11.03
Requirements
------------
* `CPython 2.7 or 3.3 <http://www.python.org>`_
* `Numpy 1.7 <http://www.numpy.org>`_
* `Matplotlib 1.3 <http://www.matplotlib.org>`_ (optional for plotting)
* `Tifffile.c 2013.01.18 <http://www.lfd.uci.edu/~gohlke/>`_
(recommended for faster decoding of PackBits and LZW encoded strings)
Notes
-----
The API is not stable yet and might change between revisions.
Tested on little-endian platforms only.
Other Python packages and modules for reading bio-scientific TIFF files:
* `Imread <http://luispedro.org/software/imread>`_
* `PyLibTiff <http://code.google.com/p/pylibtiff>`_
* `SimpleITK <http://www.simpleitk.org>`_
* `PyLSM <https://launchpad.net/pylsm>`_
* `PyMca.TiffIO.py <http://pymca.sourceforge.net/>`_
* `BioImageXD.Readers <http://www.bioimagexd.net/>`_
* `Cellcognition.io <http://cellcognition.org/>`_
* `CellProfiler.bioformats <http://www.cellprofiler.org/>`_
Acknowledgements
----------------
* Egor Zindy, University of Manchester, for cz_lsm_scan_info specifics.
* Wim Lewis for a bug fix and some read_cz_lsm functions.
* Hadrien Mary for help on reading MicroManager files.
References
----------
(1) TIFF 6.0 Specification and Supplements. Adobe Systems Incorporated.
http://partners.adobe.com/public/developer/tiff/
(2) TIFF File Format FAQ. http://www.awaresystems.be/imaging/tiff/faq.html
(3) MetaMorph Stack (STK) Image File Format.
http://support.meta.moleculardevices.com/docs/t10243.pdf
(4) File Format Description - LSM 5xx Release 2.0.
http://ibb.gsf.de/homepage/karsten.rodenacker/IDL/Lsmfile.doc
(5) BioFormats. http://www.loci.wisc.edu/ome/formats.html
(6) The OME-TIFF format.
http://www.openmicroscopy.org/site/support/file-formats/ome-tiff
(7) TiffDecoder.java
http://rsbweb.nih.gov/ij/developer/source/ij/io/TiffDecoder.java.html
(8) UltraQuant(r) Version 6.0 for Windows Start-Up Guide.
http://www.ultralum.com/images%20ultralum/pdf/UQStart%20Up%20Guide.pdf
(9) Micro-Manager File Formats.
http://www.micro-manager.org/wiki/Micro-Manager_File_Formats
Examples
--------
>>> data = numpy.random.rand(301, 219)
>>> imsave('temp.tif', data)
>>> image = imread('temp.tif')
>>> assert numpy.all(image == data)
>>> tif = TiffFile('test.tif')
>>> images = tif.asarray()
>>> image0 = tif[0].asarray()
>>> for page in tif:
... for tag in page.tags.values():
... t = tag.name, tag.value
... image = page.asarray()
... if page.is_rgb: pass
... if page.is_palette:
... t = page.color_map
... if page.is_stk:
... t = page.mm_uic_tags.number_planes
... if page.is_lsm:
... t = page.cz_lsm_info
>>> tif.close()
"""
from __future__ import division, print_function
import sys
import os
import re
import glob
import math
import zlib
import time
import json
import struct
import warnings
import datetime
import collections
from fractions import Fraction
from xml.etree import cElementTree as ElementTree
import numpy
__version__ = '2013.11.03'
__docformat__ = 'restructuredtext en'
__all__ = ['imsave', 'imread', 'imshow', 'TiffFile', 'TiffSequence']
def imsave(filename, data, photometric=None, planarconfig=None,
resolution=None, description=None, software='tifffile.py',
byteorder=None, bigtiff=False, compress=0, extratags=()):
"""Write image data to TIFF file.
Image data are written in one stripe per plane.
Dimensions larger than 2 or 3 (depending on photometric mode and
planar configuration) are flattened and saved as separate pages.
The 'sample_format' and 'bits_per_sample' TIFF tags are derived from
the data type.
Parameters
----------
filename : str
Name of file to write.
data : array_like
Input image. The last dimensions are assumed to be image height,
width, and samples.
photometric : {'minisblack', 'miniswhite', 'rgb'}
The color space of the image data.
By default this setting is inferred from the data shape.
planarconfig : {'contig', 'planar'}
Specifies if samples are stored contiguous or in separate planes.
By default this setting is inferred from the data shape.
'contig': last dimension contains samples.
'planar': third last dimension contains samples.
resolution : (float, float) or ((int, int), (int, int))
X and Y resolution in dots per inch as float or rational numbers.
description : str
The subject of the image. Saved with the first page only.
software : str
Name of the software used to create the image.
Saved with the first page only.
byteorder : {'<', '>'}
The endianness of the data in the file.
By default this is the system's native byte order.
bigtiff : bool
If True, the BigTIFF format is used.
By default the standard TIFF format is used for data less than 2000 MB.
compress : int
Values from 0 to 9 controlling the level of zlib compression.
If 0, data are written uncompressed (default).
extratags: sequence of tuples
Additional tags as [(code, dtype, count, value, writeonce)].
code : int
The TIFF tag Id.
dtype : str
Data type of items in `value` in Python struct format.
One of B, s, H, I, 2I, b, h, i, f, d, Q, or q.
count : int
Number of data values. Not used for string values.
value : sequence
`Count` values compatible with `dtype`.
writeonce : bool
If True, the tag is written to the first page only.
Examples
--------
>>> data = numpy.ones((2, 5, 3, 301, 219), 'float32') * 0.5
>>> imsave('temp.tif', data, compress=6)
>>> data = numpy.ones((5, 301, 219, 3), 'uint8') + 127
>>> value = u'{"shape": %s}' % str(list(data.shape))
>>> imsave('temp.tif', data, extratags=[(270, 's', 0, value, True)])
"""
assert(photometric in (None, 'minisblack', 'miniswhite', 'rgb'))
assert(planarconfig in (None, 'contig', 'planar'))
assert(byteorder in (None, '<', '>'))
assert(0 <= compress <= 9)
if byteorder is None:
byteorder = '<' if sys.byteorder == 'little' else '>'
data = numpy.asarray(data, dtype=byteorder+data.dtype.char, order='C')
data_shape = shape = data.shape
data = numpy.atleast_2d(data)
if not bigtiff and data.size * data.dtype.itemsize < 2000*2**20:
bigtiff = False
offset_size = 4
tag_size = 12
numtag_format = 'H'
offset_format = 'I'
val_format = '4s'
else:
bigtiff = True
offset_size = 8
tag_size = 20
numtag_format = 'Q'
offset_format = 'Q'
val_format = '8s'
# unify shape of data
samplesperpixel = 1
extrasamples = 0
if photometric is None:
if data.ndim > 2 and (shape[-3] in (3, 4) or shape[-1] in (3, 4)):
photometric = 'rgb'
else:
photometric = 'minisblack'
if photometric == 'rgb':
if len(shape) < 3:
raise ValueError("not a RGB(A) image")
if planarconfig is None:
planarconfig = 'planar' if shape[-3] in (3, 4) else 'contig'
if planarconfig == 'contig':
if shape[-1] not in (3, 4):
raise ValueError("not a contiguous RGB(A) image")
data = data.reshape((-1, 1) + shape[-3:])
samplesperpixel = shape[-1]
else:
if shape[-3] not in (3, 4):
raise ValueError("not a planar RGB(A) image")
data = data.reshape((-1, ) + shape[-3:] + (1, ))
samplesperpixel = shape[-3]
if samplesperpixel == 4:
extrasamples = 1
elif planarconfig and len(shape) > 2:
if planarconfig == 'contig':
data = data.reshape((-1, 1) + shape[-3:])
samplesperpixel = shape[-1]
else:
data = data.reshape((-1, ) + shape[-3:] + (1, ))
samplesperpixel = shape[-3]
extrasamples = samplesperpixel - 1
else:
planarconfig = None
# remove trailing 1s
while len(shape) > 2 and shape[-1] == 1:
shape = shape[:-1]
data = data.reshape((-1, 1) + shape[-2:] + (1, ))
shape = data.shape # (pages, planes, height, width, contig samples)
bytestr = bytes if sys.version[0] == '2' else (
lambda x: bytes(x, 'utf-8') if isinstance(x, str) else x)
tifftypes = {'B': 1, 's': 2, 'H': 3, 'I': 4, '2I': 5, 'b': 6,
'h': 8, 'i': 9, 'f': 11, 'd': 12, 'Q': 16, 'q': 17}
tifftags = {
'new_subfile_type': 254, 'subfile_type': 255,
'image_width': 256, 'image_length': 257, 'bits_per_sample': 258,
'compression': 259, 'photometric': 262, 'fill_order': 266,
'document_name': 269, 'image_description': 270, 'strip_offsets': 273,
'orientation': 274, 'samples_per_pixel': 277, 'rows_per_strip': 278,
'strip_byte_counts': 279, 'x_resolution': 282, 'y_resolution': 283,
'planar_configuration': 284, 'page_name': 285, 'resolution_unit': 296,
'software': 305, 'datetime': 306, 'predictor': 317, 'color_map': 320,
'extra_samples': 338, 'sample_format': 339}
tags = [] # list of (code, ifdentry, ifdvalue, writeonce)
def pack(fmt, *val):
return struct.pack(byteorder+fmt, *val)
def addtag(code, dtype, count, value, writeonce=False):
# compute ifdentry and ifdvalue bytes from code, dtype, count, value
# append (code, ifdentry, ifdvalue, writeonce) to tags list
code = tifftags[code] if code in tifftags else int(code)
if dtype not in tifftypes:
raise ValueError("unknown dtype %s" % dtype)
if dtype == 's':
value = bytestr(value) + b'\0'
count = len(value)
value = (value, )
if len(dtype) > 1:
count *= int(dtype[:-1])
dtype = dtype[-1]
ifdentry = [pack('HH', code, tifftypes[dtype]),
pack(offset_format, count)]
ifdvalue = None
if count == 1:
if isinstance(value, (tuple, list)):
value = value[0]
ifdentry.append(pack(val_format, pack(dtype, value)))
elif struct.calcsize(dtype) * count <= offset_size:
ifdentry.append(pack(val_format, pack(str(count)+dtype, *value)))
else:
ifdentry.append(pack(offset_format, 0))
ifdvalue = pack(str(count)+dtype, *value)
tags.append((code, b''.join(ifdentry), ifdvalue, writeonce))
def rational(arg, max_denominator=1000000):
# return nominator and denominator from float or two integers
try:
f = Fraction.from_float(arg)
except TypeError:
f = Fraction(arg[0], arg[1])
f = f.limit_denominator(max_denominator)
return f.numerator, f.denominator
if software:
addtag('software', 's', 0, software, writeonce=True)
if description:
addtag('image_description', 's', 0, description, writeonce=True)
elif shape != data_shape:
addtag('image_description', 's', 0,
"shape=(%s)" % (",".join('%i' % i for i in data_shape)),
writeonce=True)
addtag('datetime', 's', 0,
datetime.datetime.now().strftime("%Y:%m:%d %H:%M:%S"),
writeonce=True)
addtag('compression', 'H', 1, 32946 if compress else 1)
addtag('orientation', 'H', 1, 1)
addtag('image_width', 'I', 1, shape[-2])
addtag('image_length', 'I', 1, shape[-3])
addtag('new_subfile_type', 'I', 1, 0 if shape[0] == 1 else 2)
addtag('sample_format', 'H', 1,
{'u': 1, 'i': 2, 'f': 3, 'c': 6}[data.dtype.kind])
addtag('photometric', 'H', 1,
{'miniswhite': 0, 'minisblack': 1, 'rgb': 2}[photometric])
addtag('samples_per_pixel', 'H', 1, samplesperpixel)
if planarconfig:
addtag('planar_configuration', 'H', 1, 1 if planarconfig=='contig'
else 2)
addtag('bits_per_sample', 'H', samplesperpixel,
(data.dtype.itemsize * 8, ) * samplesperpixel)
else:
addtag('bits_per_sample', 'H', 1, data.dtype.itemsize * 8)
if extrasamples:
if photometric == 'rgb':
addtag('extra_samples', 'H', 1, 1) # alpha channel
else:
addtag('extra_samples', 'H', extrasamples, (0, ) * extrasamples)
if resolution:
addtag('x_resolution', '2I', 1, rational(resolution[0]))
addtag('y_resolution', '2I', 1, rational(resolution[1]))
addtag('resolution_unit', 'H', 1, 2)
addtag('rows_per_strip', 'I', 1, shape[-3])
# use one strip per plane
strip_byte_counts = (data[0, 0].size * data.dtype.itemsize, ) * shape[1]
addtag('strip_byte_counts', offset_format, shape[1], strip_byte_counts)
addtag('strip_offsets', offset_format, shape[1], (0, ) * shape[1])
# add extra tags from users
for t in extratags:
addtag(*t)
# the entries in an IFD must be sorted in ascending order by tag code
tags = sorted(tags, key=lambda x: x[0])
with open(filename, 'wb') as fh:
seek = fh.seek
tell = fh.tell
def write(arg, *args):
fh.write(pack(arg, *args) if args else arg)
write({'<': b'II', '>': b'MM'}[byteorder])
if bigtiff:
write('HHH', 43, 8, 0)
else:
write('H', 42)
ifd_offset = tell()
write(offset_format, 0) # first IFD
for pageindex in range(shape[0]):
# update pointer at ifd_offset
pos = tell()
seek(ifd_offset)
write(offset_format, pos)
seek(pos)
# write ifdentries
write(numtag_format, len(tags))
tag_offset = tell()
write(b''.join(t[1] for t in tags))
ifd_offset = tell()
write(offset_format, 0) # offset to next IFD
# write tag values and patch offsets in ifdentries, if necessary
for tagindex, tag in enumerate(tags):
if tag[2]:
pos = tell()
seek(tag_offset + tagindex*tag_size + offset_size + 4)
write(offset_format, pos)
seek(pos)
if tag[0] == 273:
strip_offsets_offset = pos
elif tag[0] == 279:
strip_byte_counts_offset = pos
write(tag[2])
# write image data
data_offset = tell()
if compress:
strip_byte_counts = []
for plane in data[pageindex]:
plane = zlib.compress(plane, compress)
strip_byte_counts.append(len(plane))
fh.write(plane)
else:
# if this fails try update Python/numpy
data[pageindex].tofile(fh)
fh.flush()
# update strip_offsets and strip_byte_counts if necessary
pos = tell()
for tagindex, tag in enumerate(tags):
if tag[0] == 273: # strip_offsets
if tag[2]:
seek(strip_offsets_offset)
strip_offset = data_offset
for size in strip_byte_counts:
write(offset_format, strip_offset)
strip_offset += size
else:
seek(tag_offset + tagindex*tag_size + offset_size + 4)
write(offset_format, data_offset)
elif tag[0] == 279: # strip_byte_counts
if compress:
if tag[2]:
seek(strip_byte_counts_offset)
for size in strip_byte_counts:
write(offset_format, size)
else:
seek(tag_offset + tagindex*tag_size +
offset_size + 4)
write(offset_format, strip_byte_counts[0])
break
seek(pos)
fh.flush()
# remove tags that should be written only once
if pageindex == 0:
tags = [t for t in tags if not t[-1]]
def imread(files, *args, **kwargs):
"""Return image data from TIFF file(s) as numpy array.
The first image series is returned if no arguments are provided.
Parameters
----------
files : str or list
File name, glob pattern, or list of file names.
key : int, slice, or sequence of page indices
Defines which pages to return as array.
series : int
Defines which series of pages in file to return as array.
multifile : bool
If True (default), OME-TIFF data may include pages from multiple files.
pattern : str
Regular expression pattern that matches axes names and indices in
file names.
Examples
--------
>>> im = imread('test.tif', 0)
>>> im.shape
(256, 256, 4)
>>> ims = imread(['test.tif', 'test.tif'])
>>> ims.shape
(2, 256, 256, 4)
"""
kwargs_file = {}
if 'multifile' in kwargs:
kwargs_file['multifile'] = kwargs['multifile']
del kwargs['multifile']
else:
kwargs_file['multifile'] = True
kwargs_seq = {}
if 'pattern' in kwargs:
kwargs_seq['pattern'] = kwargs['pattern']
del kwargs['pattern']
if isinstance(files, basestring) and any(i in files for i in '?*'):
files = glob.glob(files)
if not files:
raise ValueError('no files found')
if len(files) == 1:
files = files[0]
if isinstance(files, basestring):
with TiffFile(files, **kwargs_file) as tif:
return tif.asarray(*args, **kwargs)
else:
with TiffSequence(files, **kwargs_seq) as imseq:
return imseq.asarray(*args, **kwargs)
class lazyattr(object):
"""Lazy object attribute whose value is computed on first access."""
__slots__ = ('func', )
def __init__(self, func):
self.func = func
def __get__(self, instance, owner):
if instance is None:
return self
value = self.func(instance)
if value is NotImplemented:
return getattr(super(owner, instance), self.func.__name__)
setattr(instance, self.func.__name__, value)
return value
class TiffFile(object):
"""Read image and meta-data from TIFF, STK, LSM, and FluoView files.
TiffFile instances must be closed using the close method, which is
automatically called when using the 'with' statement.
Attributes
----------
pages : list
All TIFF pages in file.
series : list of Records(shape, dtype, axes, TiffPages)
TIFF pages with compatible shapes and types.
micromanager_metadata: dict
Extra MicroManager non-TIFF metadata in the file, if exists.
All attributes are read-only.
Examples
--------
>>> tif = TiffFile('test.tif')
... try:
... images = tif.asarray()
... except Exception as e:
... print(e)
... finally:
... tif.close()
"""
def __init__(self, arg, name=None, multifile=False):
"""Initialize instance from file.
Parameters
----------
arg : str or open file
Name of file or open file object.
The file objects are closed in TiffFile.close().
name : str
Human readable label of open file.
multifile : bool
If True, series may include pages from multiple files.
"""
if isinstance(arg, basestring):
filename = os.path.abspath(arg)
self._fh = open(filename, 'rb')
else:
filename = str(name)
self._fh = arg
self._fh.seek(0, 2)
self._fsize = self._fh.tell()
self._fh.seek(0)
self.fname = os.path.basename(filename)
self.fpath = os.path.dirname(filename)
self._tiffs = {self.fname: self} # cache of TiffFiles
self.offset_size = None
self.pages = []
self._multifile = bool(multifile)
try:
self._fromfile()
except Exception:
self._fh.close()
raise
def close(self):
"""Close open file handle(s)."""
for tif in self._tiffs.values():
if tif._fh:
tif._fh.close()
tif._fh = None
self._tiffs = {}
def _fromfile(self):
"""Read TIFF header and all page records from file."""
self._fh.seek(0)
try:
self.byteorder = {b'II': '<', b'MM': '>'}[self._fh.read(2)]
except KeyError:
raise ValueError("not a valid TIFF file")
version = struct.unpack(self.byteorder+'H', self._fh.read(2))[0]
if version == 43: # BigTiff
self.offset_size, zero = struct.unpack(self.byteorder+'HH',
self._fh.read(4))
if zero or self.offset_size != 8:
raise ValueError("not a valid BigTIFF file")
elif version == 42:
self.offset_size = 4
else:
raise ValueError("not a TIFF file")
self.pages = []
while True:
try:
page = TiffPage(self)
self.pages.append(page)
except StopIteration:
break
if not self.pages:
raise ValueError("empty TIFF file")
if self.is_micromanager:
# MicroManager files contain metadata not stored in TIFF tags.
self.micromanager_metadata = read_micromanager_metadata(self._fh)
@lazyattr
def series(self):
"""Return series of TiffPage with compatible shape and properties."""
series = []
if self.is_ome:
series = self._omeseries()
elif self.is_fluoview:
dims = {b'X': 'X', b'Y': 'Y', b'Z': 'Z', b'T': 'T',
b'WAVELENGTH': 'C', b'TIME': 'T', b'XY': 'R',
b'EVENT': 'V', b'EXPOSURE': 'L'}
mmhd = list(reversed(self.pages[0].mm_header.dimensions))
series = [Record(
axes=''.join(dims.get(i[0].strip().upper(), 'Q')
for i in mmhd if i[1] > 1),
shape=tuple(int(i[1]) for i in mmhd if i[1] > 1),
pages=self.pages, dtype=numpy.dtype(self.pages[0].dtype))]
elif self.is_lsm:
lsmi = self.pages[0].cz_lsm_info
axes = CZ_SCAN_TYPES[lsmi.scan_type]
if self.pages[0].is_rgb:
axes = axes.replace('C', '').replace('XY', 'XYC')
axes = axes[::-1]
shape = [getattr(lsmi, CZ_DIMENSIONS[i]) for i in axes]
pages = [p for p in self.pages if not p.is_reduced]
series = [Record(axes=axes, shape=shape, pages=pages,
dtype=numpy.dtype(pages[0].dtype))]
if len(pages) != len(self.pages): # reduced RGB pages
pages = [p for p in self.pages if p.is_reduced]
cp = 1
i = 0
while cp < len(pages) and i < len(shape)-2:
cp *= shape[i]
i += 1
shape = shape[:i] + list(pages[0].shape)
axes = axes[:i] + 'CYX'
series.append(Record(axes=axes, shape=shape, pages=pages,
dtype=numpy.dtype(pages[0].dtype)))
elif self.is_imagej:
shape = []
axes = []
ij = self.pages[0].imagej_tags
if 'frames' in ij:
shape.append(ij['frames'])
axes.append('T')
if 'slices' in ij:
shape.append(ij['slices'])
axes.append('Z')
if 'channels' in ij and not self.is_rgb:
shape.append(ij['channels'])
axes.append('C')
remain = len(self.pages) // (numpy.prod(shape) if shape else 1)
if remain > 1:
shape.append(remain)
axes.append('I')
shape.extend(self.pages[0].shape)
axes.extend(self.pages[0].axes)
axes = ''.join(axes)
series = [Record(pages=self.pages, shape=shape, axes=axes,
dtype=numpy.dtype(self.pages[0].dtype))]
elif self.is_nih:
series = [Record(pages=self.pages,
shape=(len(self.pages),) + self.pages[0].shape,
axes='I' + self.pages[0].axes,
dtype=numpy.dtype(self.pages[0].dtype))]
elif self.pages[0].is_shaped:
shape = self.pages[0].tags['image_description'].value[7:-1]
shape = tuple(int(i) for i in shape.split(b','))
series = [Record(pages=self.pages, shape=shape,
axes='Q' * len(shape),
dtype=numpy.dtype(self.pages[0].dtype))]
if not series:
shapes = []
pages = {}
for page in self.pages:
if not page.shape:
continue
shape = page.shape + (page.axes,
page.compression in TIFF_DECOMPESSORS)
if not shape in pages:
shapes.append(shape)
pages[shape] = [page]
else:
pages[shape].append(page)
series = [Record(pages=pages[s],
axes=(('I' + s[-2])
if len(pages[s]) > 1 else s[-2]),
dtype=numpy.dtype(pages[s][0].dtype),
shape=((len(pages[s]), ) + s[:-2]
if len(pages[s]) > 1 else s[:-2]))
for s in shapes]
return series
def asarray(self, key=None, series=None, memmap=False):
"""Return image data of multiple TIFF pages as numpy array.
By default the first image series is returned.
Parameters
----------
key : int, slice, or sequence of page indices
Defines which pages to return as array.
series : int
Defines which series of pages to return as array.
memmap : bool
If True, use numpy.memmap to read arrays from file if possible.
"""
if key is None and series is None:
series = 0
if series is not None:
pages = self.series[series].pages
else:
pages = self.pages
if key is None:
pass
elif isinstance(key, int):
pages = [pages[key]]
elif isinstance(key, slice):
pages = pages[key]
elif isinstance(key, collections.Iterable):
pages = [pages[k] for k in key]
else:
raise TypeError("key must be an int, slice, or sequence")
if len(pages) == 1:
return pages[0].asarray(memmap=memmap)
elif self.is_nih:
result = numpy.vstack(
p.asarray(colormapped=False, squeeze=False, memmap=memmap)
for p in pages)
if pages[0].is_palette:
result = numpy.take(pages[0].color_map, result, axis=1)
result = numpy.swapaxes(result, 0, 1)
else:
if self.is_ome and any(p is None for p in pages):
firstpage = next(p for p in pages if p)
nopage = numpy.zeros_like(firstpage.asarray(memmap=memmap))
result = numpy.vstack((p.asarray(memmap=memmap) if p else nopage)
for p in pages)
if key is None:
try:
result.shape = self.series[series].shape
except ValueError:
warnings.warn("failed to reshape %s to %s" % (
result.shape, self.series[series].shape))
result.shape = (-1,) + pages[0].shape
else:
result.shape = (-1,) + pages[0].shape
return result
def _omeseries(self):
"""Return image series in OME-TIFF file(s)."""
root = ElementTree.XML(self.pages[0].tags['image_description'].value)
uuid = root.attrib.get('UUID', None)
self._tiffs = {uuid: self}
modulo = {}
result = []
for element in root:
if element.tag.endswith('BinaryOnly'):
warnings.warn("not an OME-TIFF master file")
break
if element.tag.endswith('StructuredAnnotations'):
for annot in element:
if not annot.attrib.get('Namespace',
'').endswith('modulo'):
continue
for value in annot:
for modul in value:
for along in modul:
if not along.tag[:-1].endswith('Along'):
continue
axis = along.tag[-1]
newaxis = along.attrib.get('Type', 'other')
newaxis = AXES_LABELS[newaxis]
if 'Start' in along.attrib:
labels = range(
int(along.attrib['Start']),
int(along.attrib['End']) + 1,
int(along.attrib.get('Step', 1)))
else:
labels = [label.text for label in along
if label.tag.endswith('Label')]
modulo[axis] = (newaxis, labels)
if not element.tag.endswith('Image'):
continue
for pixels in element:
if not pixels.tag.endswith('Pixels'):
continue
atr = pixels.attrib
axes = "".join(reversed(atr['DimensionOrder']))
shape = list(int(atr['Size'+ax]) for ax in axes)
size = numpy.prod(shape[:-2])
ifds = [None] * size
for data in pixels:
if not data.tag.endswith('TiffData'):
continue
atr = data.attrib
ifd = int(atr.get('IFD', 0))
num = int(atr.get('NumPlanes', 1 if 'IFD' in atr else 0))
num = int(atr.get('PlaneCount', num))
idx = [int(atr.get('First'+ax, 0)) for ax in axes[:-2]]
idx = numpy.ravel_multi_index(idx, shape[:-2])
for uuid in data:
if uuid.tag.endswith('UUID'):
if uuid.text not in self._tiffs:
if not self._multifile:
# abort reading multi file OME series
return []
fn = uuid.attrib['FileName']
try:
tf = TiffFile(os.path.join(self.fpath, fn))
except (IOError, ValueError):
warnings.warn("failed to read %s" % fn)
break
self._tiffs[uuid.text] = tf
pages = self._tiffs[uuid.text].pages
try:
for i in range(num if num else len(pages)):
ifds[idx + i] = pages[ifd + i]
except IndexError:
warnings.warn("ome-xml: index out of range")
break
else:
pages = self.pages
try:
for i in range(num if num else len(pages)):
ifds[idx + i] = pages[ifd + i]
except IndexError:
warnings.warn("ome-xml: index out of range")
result.append(Record(axes=axes, shape=shape, pages=ifds,
dtype=numpy.dtype(ifds[0].dtype)))
for record in result:
for axis, (newaxis, labels) in modulo.items():
i = record.axes.index(axis)
size = len(labels)
if record.shape[i] == size:
record.axes = record.axes.replace(axis, newaxis, 1)
else:
record.shape[i] //= size
record.shape.insert(i+1, size)
record.axes = record.axes.replace(axis, axis+newaxis, 1)
return result
def __len__(self):
"""Return number of image pages in file."""
return len(self.pages)
def __getitem__(self, key):
"""Return specified page."""
return self.pages[key]
def __iter__(self):
"""Return iterator over pages."""
return iter(self.pages)
def __str__(self):
"""Return string containing information about file."""
result = [
self.fname.capitalize(),
format_size(self._fsize),
{'<': 'little endian', '>': 'big endian'}[self.byteorder]]
if self.is_bigtiff:
result.append("bigtiff")
if len(self.pages) > 1:
result.append("%i pages" % len(self.pages))
if len(self.series) > 1:
result.append("%i series" % len(self.series))
if len(self._tiffs) > 1:
result.append("%i files" % (len(self._tiffs)))
return ", ".join(result)
def __enter__(self):
return self
def __exit__(self, exc_type, exc_value, traceback):
self.close()
@lazyattr
def fstat(self):
try:
return os.fstat(self._fh.fileno())
except Exception: # io.UnsupportedOperation
return None
@lazyattr
def is_bigtiff(self):
return self.offset_size != 4
@lazyattr
def is_rgb(self):
return all(p.is_rgb for p in self.pages)
@lazyattr
def is_palette(self):
return all(p.is_palette for p in self.pages)
@lazyattr
def is_mdgel(self):
return any(p.is_mdgel for p in self.pages)
@lazyattr
def is_mediacy(self):
return any(p.is_mediacy for p in self.pages)
@lazyattr
def is_stk(self):
return all(p.is_stk for p in self.pages)
@lazyattr
def is_lsm(self):
return self.pages[0].is_lsm
@lazyattr
def is_imagej(self):
return self.pages[0].is_imagej
@lazyattr
def is_micromanager(self):
return self.pages[0].is_micromanager
@lazyattr
def is_nih(self):
return self.pages[0].is_nih
@lazyattr
def is_fluoview(self):
return self.pages[0].is_fluoview
@lazyattr
def is_ome(self):
return self.pages[0].is_ome
class TiffPage(object):
"""A TIFF image file directory (IFD).
Attributes
----------
index : int
Index of page in file.
dtype : str {TIFF_SAMPLE_DTYPES}
Data type of image, colormapped if applicable.
shape : tuple
Dimensions of the image array in TIFF page,
colormapped and with one alpha channel if applicable.
axes : str
Axes label codes:
'X' width, 'Y' height, 'S' sample, 'P' plane, 'I' image series,
'Z' depth, 'C' color|em-wavelength|channel, 'E' ex-wavelength|lambda,
'T' time, 'R' region|tile, 'A' angle, 'F' phase, 'H' lifetime,
'L' exposure, 'V' event, 'Q' unknown, '_' missing
tags : TiffTags
Dictionary of tags in page.
Tag values are also directly accessible as attributes.
color_map : numpy array
Color look up table, if exists.
mm_uic_tags: Record(dict)
Consolidated MetaMorph mm_uic# tags, if exists.
cz_lsm_scan_info: Record(dict)
LSM scan info attributes, if exists.
imagej_tags: Record(dict)
Consolidated ImageJ description and metadata tags, if exists.
All attributes are read-only.
"""
def __init__(self, parent):
"""Initialize instance from file."""
self.parent = parent
self.index = len(parent.pages)
self.shape = self._shape = ()
self.dtype = self._dtype = None
self.axes = ""
self.tags = TiffTags()
self._fromfile()
self._process_tags()
def _fromfile(self):
"""Read TIFF IFD structure and its tags from file.
File cursor must be at storage position of IFD offset and is left at
offset to next IFD.
Raises StopIteration if offset (first bytes read) is 0.
"""
fh = self.parent._fh
byteorder = self.parent.byteorder
offset_size = self.parent.offset_size
fmt = {4: 'I', 8: 'Q'}[offset_size]
offset = struct.unpack(byteorder + fmt, fh.read(offset_size))[0]
if not offset:
raise StopIteration()
# read standard tags
tags = self.tags
fh.seek(offset)
fmt, size = {4: ('H', 2), 8: ('Q', 8)}[offset_size]
try:
numtags = struct.unpack(byteorder + fmt, fh.read(size))[0]
except Exception:
warnings.warn("corrupted page list")
raise StopIteration()
tagcode = 0
for _ in range(numtags):
try:
tag = TiffTag(self.parent)
except TiffTag.Error as e:
warnings.warn(str(e))
finally:
if tagcode > tag.code:
warnings.warn("tags are not ordered by code")
tagcode = tag.code
if not tag.name in tags:
tags[tag.name] = tag
else:
# some files contain multiple IFD with same code
# e.g. MicroManager files contain two image_description
for ext in ('_1', '_2', '_3'):
name = tag.name + ext
if not name in tags:
tags[name] = tag
break
# read LSM info subrecords
if self.is_lsm:
pos = fh.tell()
for name, reader in CZ_LSM_INFO_READERS.items():
try:
offset = self.cz_lsm_info['offset_'+name]
except KeyError:
continue
if not offset:
continue
fh.seek(offset)
try:
setattr(self, 'cz_lsm_'+name, reader(fh, byteorder))
except ValueError:
pass
fh.seek(pos)
def _process_tags(self):
"""Validate standard tags and initialize attributes.
Raise ValueError if tag values are not supported.
"""
tags = self.tags
for code, (name, default, dtype, count, validate) in TIFF_TAGS.items():
if not (name in tags or default is None):
tags[name] = TiffTag(code, dtype=dtype, count=count,
value=default, name=name)
if name in tags and validate:
try:
if tags[name].count == 1:
setattr(self, name, validate[tags[name].value])
else:
setattr(self, name, tuple(
validate[value] for value in tags[name].value))
except KeyError:
raise ValueError("%s.value (%s) not supported" %
(name, tags[name].value))
tag = tags['bits_per_sample']
if tag.count == 1:
self.bits_per_sample = tag.value
else:
value = tag.value[:self.samples_per_pixel]
if any((v-value[0] for v in value)):
self.bits_per_sample = value
else:
self.bits_per_sample = value[0]
tag = tags['sample_format']
if tag.count == 1:
self.sample_format = TIFF_SAMPLE_FORMATS[tag.value]
else:
value = tag.value[:self.samples_per_pixel]
if any((v-value[0] for v in value)):
self.sample_format = [TIFF_SAMPLE_FORMATS[v] for v in value]
else:
self.sample_format = TIFF_SAMPLE_FORMATS[value[0]]
if not 'photometric' in tags:
self.photometric = None
if 'image_length' in tags:
self.strips_per_image = int(math.floor(
float(self.image_length + self.rows_per_strip - 1) /
self.rows_per_strip))
else:
self.strips_per_image = 0
key = (self.sample_format, self.bits_per_sample)
self.dtype = self._dtype = TIFF_SAMPLE_DTYPES.get(key, None)
if self.is_imagej:
# consolidate imagej meta data
if 'image_description_1' in self.tags: # MicroManager
adict = imagej_description(tags['image_description_1'].value)
else:
adict = imagej_description(tags['image_description'].value)
if 'imagej_metadata' in tags:
try:
adict.update(imagej_metadata(
tags['imagej_metadata'].value,
tags['imagej_byte_counts'].value,
self.parent.byteorder))
except Exception as e:
warnings.warn(str(e))
self.imagej_tags = Record(adict)
if not 'image_length' in self.tags or not 'image_width' in self.tags:
# some GEL file pages are missing image data
self.image_length = 0
self.image_width = 0
self.strip_offsets = 0
self._shape = ()
self.shape = ()
self.axes = ''
if self.is_palette:
self.dtype = self.tags['color_map'].dtype[1]
self.color_map = numpy.array(self.color_map, self.dtype)
dmax = self.color_map.max()
if dmax < 256:
self.dtype = numpy.uint8
self.color_map = self.color_map.astype(self.dtype)
#else:
# self.dtype = numpy.uint8
# self.color_map >>= 8
# self.color_map = self.color_map.astype(self.dtype)
self.color_map.shape = (3, -1)
if self.is_stk:
# consolidate mm_uci tags
planes = tags['mm_uic2'].count
self.mm_uic_tags = Record(tags['mm_uic2'].value)
for key in ('mm_uic3', 'mm_uic4', 'mm_uic1'):
if key in tags:
self.mm_uic_tags.update(tags[key].value)
if self.planar_configuration == 'contig':
self._shape = (planes, 1, self.image_length, self.image_width,
self.samples_per_pixel)
self.shape = tuple(self._shape[i] for i in (0, 2, 3, 4))
self.axes = 'PYXS'
else:
self._shape = (planes, self.samples_per_pixel,
self.image_length, self.image_width, 1)
self.shape = self._shape[:4]
self.axes = 'PSYX'
if self.is_palette and (self.color_map.shape[1]
>= 2**self.bits_per_sample):
self.shape = (3, planes, self.image_length, self.image_width)
self.axes = 'CPYX'
else:
warnings.warn("palette cannot be applied")
self.is_palette = False
elif self.is_palette:
samples = 1
if 'extra_samples' in self.tags:
samples += len(self.extra_samples)
if self.planar_configuration == 'contig':
self._shape = (
1, 1, self.image_length, self.image_width, samples)
else:
self._shape = (
1, samples, self.image_length, self.image_width, 1)
if self.color_map.shape[1] >= 2**self.bits_per_sample:
self.shape = (3, self.image_length, self.image_width)
self.axes = 'CYX'
else:
warnings.warn("palette cannot be applied")
self.is_palette = False
self.shape = (self.image_length, self.image_width)
self.axes = 'YX'
elif self.is_rgb or self.samples_per_pixel > 1:
if self.planar_configuration == 'contig':
self._shape = (1, 1, self.image_length, self.image_width,
self.samples_per_pixel)
self.shape = (self.image_length, self.image_width,
self.samples_per_pixel)
self.axes = 'YXS'
else:
self._shape = (1, self.samples_per_pixel, self.image_length,
self.image_width, 1)
self.shape = self._shape[1:-1]
self.axes = 'SYX'
if self.is_rgb and 'extra_samples' in self.tags:
extra_samples = self.extra_samples
if self.tags['extra_samples'].count == 1:
extra_samples = (extra_samples, )
for exs in extra_samples:
if exs in ('unassalpha', 'assocalpha', 'unspecified'):
if self.planar_configuration == 'contig':
self.shape = self.shape[:2] + (4,)
else:
self.shape = (4,) + self.shape[1:]
break
else:
self._shape = (1, 1, self.image_length, self.image_width, 1)
self.shape = self._shape[2:4]
self.axes = 'YX'
if not self.compression and not 'strip_byte_counts' in tags:
self.strip_byte_counts = numpy.prod(self.shape) * (
self.bits_per_sample // 8)
def asarray(self, squeeze=True, colormapped=True, rgbonly=True,
memmap=False):
"""Read image data from file and return as numpy array.
Raise ValueError if format is unsupported.
If any argument is False, the shape of the returned array might be
different from the page shape.
Parameters
----------
squeeze : bool
If True, all length-1 dimensions (except X and Y) are
squeezed out from result.
colormapped : bool
If True, color mapping is applied for palette-indexed images.
rgbonly : bool
If True, return RGB(A) image without additional extra samples.
memmap : bool
If True, use numpy.memmap to read array if possible.
"""
fh = self.parent._fh
if not fh:
raise IOError("TIFF file is not open")
if self.dtype is None:
raise ValueError("data type not supported: %s%i" % (
self.sample_format, self.bits_per_sample))
if self.compression not in TIFF_DECOMPESSORS:
raise ValueError("cannot decompress %s" % self.compression)
if ('ycbcr_subsampling' in self.tags
and self.tags['ycbcr_subsampling'].value not in (1, (1, 1))):
raise ValueError("YCbCr subsampling not supported")
tag = self.tags['sample_format']
if tag.count != 1 and any((i-tag.value[0] for i in tag.value)):
raise ValueError("sample formats don't match %s" % str(tag.value))
dtype = self._dtype
shape = self._shape
if not shape:
return None
image_width = self.image_width
image_length = self.image_length
typecode = self.parent.byteorder + dtype
bits_per_sample = self.bits_per_sample
byteorder_is_native = ({'big': '>', 'little': '<'}[sys.byteorder] ==
self.parent.byteorder)
if self.is_tiled:
if 'tile_offsets' in self.tags:
byte_counts = self.tile_byte_counts
offsets = self.tile_offsets
else:
byte_counts = self.strip_byte_counts
offsets = self.strip_offsets
tile_width = self.tile_width
tile_length = self.tile_length
tw = (image_width + tile_width - 1) // tile_width
tl = (image_length + tile_length - 1) // tile_length
shape = shape[:-3] + (tl*tile_length, tw*tile_width, shape[-1])
tile_shape = (tile_length, tile_width, shape[-1])
runlen = tile_width
else:
byte_counts = self.strip_byte_counts
offsets = self.strip_offsets
runlen = image_width
try:
offsets[0]
except TypeError:
offsets = (offsets, )
byte_counts = (byte_counts, )
if any(o < 2 for o in offsets):
raise ValueError("corrupted page")
if (not self.is_tiled and (self.is_stk or (not self.compression
and bits_per_sample in (8, 16, 32, 64)
and all(offsets[i] == offsets[i+1] - byte_counts[i]
for i in range(len(offsets)-1))))):
# contiguous data
if (memmap and not (self.is_tiled or self.predictor or
('extra_samples' in self.tags) or
(colormapped and self.is_palette) or
(not byteorder_is_native))):
result = numpy.memmap(fh, typecode, 'r', offsets[0], shape)
else:
fh.seek(offsets[0])
result = numpy_fromfile(fh, typecode, numpy.prod(shape))
result = result.astype('=' + dtype)
else:
if self.planar_configuration == 'contig':
runlen *= self.samples_per_pixel
if bits_per_sample in (8, 16, 32, 64, 128):
if (bits_per_sample * runlen) % 8:
raise ValueError("data and sample size mismatch")
def unpack(x):
return numpy.fromstring(x, typecode)
elif isinstance(bits_per_sample, tuple):
def unpack(x):
return unpackrgb(x, typecode, bits_per_sample)
else:
def unpack(x):
return unpackints(x, typecode, bits_per_sample, runlen)
decompress = TIFF_DECOMPESSORS[self.compression]
if self.is_tiled:
result = numpy.empty(shape, dtype)
tw, tl, pl = 0, 0, 0
for offset, bytecount in zip(offsets, byte_counts):
fh.seek(offset)
tile = unpack(decompress(fh.read(bytecount)))
tile.shape = tile_shape
if self.predictor == 'horizontal':
numpy.cumsum(tile, axis=-2, dtype=dtype, out=tile)
result[0, pl, tl:tl+tile_length,
tw:tw+tile_width, :] = tile
del tile
tw += tile_width
if tw >= shape[-2]:
tw, tl = 0, tl + tile_length
if tl >= shape[-3]:
tl, pl = 0, pl + 1
result = result[..., :image_length, :image_width, :]
else:
strip_size = (self.rows_per_strip * self.image_width *
self.samples_per_pixel)
result = numpy.empty(shape, dtype).reshape(-1)
index = 0
for offset, bytecount in zip(offsets, byte_counts):
fh.seek(offset)
strip = fh.read(bytecount)
strip = unpack(decompress(strip))
size = min(result.size, strip.size, strip_size,
result.size - index)
result[index:index+size] = strip[:size]
del strip
index += size
result.shape = self._shape
if self.predictor == 'horizontal' and not self.is_tiled:
# work around bug in LSM510 software
if not (self.parent.is_lsm and not self.compression):
numpy.cumsum(result, axis=-2, dtype=dtype, out=result)
if colormapped and self.is_palette:
if self.color_map.shape[1] >= 2**bits_per_sample:
# FluoView and LSM might fail here
result = numpy.take(self.color_map,
result[:, 0, :, :, 0], axis=1)
elif rgbonly and self.is_rgb and 'extra_samples' in self.tags:
# return only RGB and first alpha channel if exists
extra_samples = self.extra_samples
if self.tags['extra_samples'].count == 1:
extra_samples = (extra_samples, )
for i, exs in enumerate(extra_samples):
if exs in ('unassalpha', 'assocalpha', 'unspecified'):
if self.planar_configuration == 'contig':
result = result[..., [0, 1, 2, 3+i]]
else:
result = result[:, [0, 1, 2, 3+i]]
break
else:
if self.planar_configuration == 'contig':
result = result[..., :3]
else:
result = result[:, :3]
if squeeze:
try:
result.shape = self.shape
except ValueError:
warnings.warn("failed to reshape from %s to %s" % (
str(result.shape), str(self.shape)))
return result
def __str__(self):
"""Return string containing information about page."""
s = ', '.join(s for s in (
' x '.join(str(i) for i in self.shape),
str(numpy.dtype(self.dtype)),
'%s bit' % str(self.bits_per_sample),
self.photometric if 'photometric' in self.tags else '',
self.compression if self.compression else 'raw',
'|'.join(t[3:] for t in (
'is_stk', 'is_lsm', 'is_nih', 'is_ome', 'is_imagej',
'is_micromanager', 'is_fluoview', 'is_mdgel', 'is_mediacy',
'is_reduced', 'is_tiled') if getattr(self, t))) if s)
return "Page %i: %s" % (self.index, s)
def __getattr__(self, name):
"""Return tag value."""
if name in self.tags:
value = self.tags[name].value
setattr(self, name, value)
return value
raise AttributeError(name)
@lazyattr
def is_rgb(self):
"""True if page contains a RGB image."""
return ('photometric' in self.tags and
self.tags['photometric'].value == 2)
@lazyattr
def is_palette(self):
"""True if page contains a palette-colored image."""
return ('photometric' in self.tags and
self.tags['photometric'].value == 3)
@lazyattr
def is_tiled(self):
"""True if page contains tiled image."""
return 'tile_width' in self.tags
@lazyattr
def is_reduced(self):
"""True if page is a reduced image of another image."""
return bool(self.tags['new_subfile_type'].value & 1)
@lazyattr
def is_mdgel(self):
"""True if page contains md_file_tag tag."""
return 'md_file_tag' in self.tags
@lazyattr
def is_mediacy(self):
"""True if page contains Media Cybernetics Id tag."""
return ('mc_id' in self.tags and
self.tags['mc_id'].value.startswith(b'MC TIFF'))
@lazyattr
def is_stk(self):
"""True if page contains MM_UIC2 tag."""
return 'mm_uic2' in self.tags
@lazyattr
def is_lsm(self):
"""True if page contains LSM CZ_LSM_INFO tag."""
return 'cz_lsm_info' in self.tags
@lazyattr
def is_fluoview(self):
"""True if page contains FluoView MM_STAMP tag."""
return 'mm_stamp' in self.tags
@lazyattr
def is_nih(self):
"""True if page contains NIH image header."""
return 'nih_image_header' in self.tags
@lazyattr
def is_ome(self):
"""True if page contains OME-XML in image_description tag."""
return ('image_description' in self.tags and self.tags[
'image_description'].value.startswith(b'<?xml version='))
@lazyattr
def is_shaped(self):
"""True if page contains shape in image_description tag."""
return ('image_description' in self.tags and self.tags[
'image_description'].value.startswith(b'shape=('))
@lazyattr
def is_imagej(self):
"""True if page contains ImageJ description."""
return (
('image_description' in self.tags and
self.tags['image_description'].value.startswith(b'ImageJ=')) or
('image_description_1' in self.tags and # Micromanager
self.tags['image_description_1'].value.startswith(b'ImageJ=')))
@lazyattr
def is_micromanager(self):
"""True if page contains Micro-Manager metadata."""
return 'micromanager_metadata' in self.tags
class TiffTag(object):
"""A TIFF tag structure.
Attributes
----------
name : string
Attribute name of tag.
code : int
Decimal code of tag.
dtype : str
Datatype of tag data. One of TIFF_DATA_TYPES.
count : int
Number of values.
value : various types
Tag data as Python object.
value_offset : int
Location of value in file, if any.
All attributes are read-only.
"""
__slots__ = ('code', 'name', 'count', 'dtype', 'value', 'value_offset',
'_offset', '_value')
class Error(Exception):
pass
def __init__(self, arg, **kwargs):
"""Initialize instance from file or arguments."""
self._offset = None
if hasattr(arg, '_fh'):
self._fromfile(arg, **kwargs)
else:
self._fromdata(arg, **kwargs)
def _fromdata(self, code, dtype, count, value, name=None):
"""Initialize instance from arguments."""
self.code = int(code)
self.name = name if name else str(code)
self.dtype = TIFF_DATA_TYPES[dtype]
self.count = int(count)
self.value = value
def _fromfile(self, parent):
"""Read tag structure from open file. Advance file cursor."""
fh = parent._fh
byteorder = parent.byteorder
self._offset = fh.tell()
self.value_offset = self._offset + parent.offset_size + 4
fmt, size = {4: ('HHI4s', 12), 8: ('HHQ8s', 20)}[parent.offset_size]
data = fh.read(size)
code, dtype = struct.unpack(byteorder + fmt[:2], data[:4])
count, value = struct.unpack(byteorder + fmt[2:], data[4:])
self._value = value
if code in TIFF_TAGS:
name = TIFF_TAGS[code][0]
elif code in CUSTOM_TAGS:
name = CUSTOM_TAGS[code][0]
else:
name = str(code)
try:
dtype = TIFF_DATA_TYPES[dtype]
except KeyError:
raise TiffTag.Error("unknown tag data type %i" % dtype)
fmt = '%s%i%s' % (byteorder, count*int(dtype[0]), dtype[1])
size = struct.calcsize(fmt)
if size > parent.offset_size or code in CUSTOM_TAGS:
pos = fh.tell()
tof = {4: 'I', 8: 'Q'}[parent.offset_size]
self.value_offset = offset = struct.unpack(byteorder+tof, value)[0]
if offset < 0 or offset > parent._fsize:
raise TiffTag.Error("corrupt file - invalid tag value offset")
elif offset < 4:
raise TiffTag.Error("corrupt value offset for tag %i" % code)
fh.seek(offset)
if code in CUSTOM_TAGS:
readfunc = CUSTOM_TAGS[code][1]
value = readfunc(fh, byteorder, dtype, count)
fh.seek(0, 2) # bug in numpy/Python 3.x ?
if isinstance(value, dict): # numpy.core.records.record
value = Record(value)
elif code in TIFF_TAGS or dtype[-1] == 's':
value = struct.unpack(fmt, fh.read(size))
else:
value = read_numpy(fh, byteorder, dtype, count)
fh.seek(0, 2) # bug in numpy/Python 3.x ?
fh.seek(pos)
else:
value = struct.unpack(fmt, value[:size])
if not code in CUSTOM_TAGS:
if len(value) == 1:
value = value[0]
if dtype.endswith('s') and isinstance(value, bytes):
value = stripnull(value)
self.code = code
self.name = name
self.dtype = dtype
self.count = count
self.value = value
def __str__(self):
"""Return string containing information about tag."""
return ' '.join(str(getattr(self, s)) for s in self.__slots__)
class TiffSequence(object):
"""Sequence of image files.
Properties
----------
files : list
List of file names.
shape : tuple
Shape of image sequence.
axes : str
Labels of axes in shape.
Examples
--------
>>> ims = TiffSequence("test.oif.files/*.tif")
>>> ims = ims.asarray()
>>> ims.shape
(2, 100, 256, 256)
"""
_axes_pattern = """
# matches Olympus OIF and Leica TIFF series
_?(?:(q|l|p|a|c|t|x|y|z|ch|tp)(\d{1,4}))
_?(?:(q|l|p|a|c|t|x|y|z|ch|tp)(\d{1,4}))?
_?(?:(q|l|p|a|c|t|x|y|z|ch|tp)(\d{1,4}))?
_?(?:(q|l|p|a|c|t|x|y|z|ch|tp)(\d{1,4}))?
_?(?:(q|l|p|a|c|t|x|y|z|ch|tp)(\d{1,4}))?
_?(?:(q|l|p|a|c|t|x|y|z|ch|tp)(\d{1,4}))?
_?(?:(q|l|p|a|c|t|x|y|z|ch|tp)(\d{1,4}))?
"""
class _ParseError(Exception):
pass
def __init__(self, files, imread=TiffFile, pattern='axes'):
"""Initialize instance from multiple files.
Parameters
----------
files : str, or sequence of str
Glob pattern or sequence of file names.
imread : function or class
Image read function or class with asarray function returning numpy
array from single file.
pattern : str
Regular expression pattern that matches axes names and sequence
indices in file names.
"""
if isinstance(files, basestring):
files = natural_sorted(glob.glob(files))
files = list(files)
if not files:
raise ValueError("no files found")
#if not os.path.isfile(files[0]):
# raise ValueError("file not found")
self.files = files
if hasattr(imread, 'asarray'):
_imread = imread
def imread(fname, *args, **kwargs):
with _imread(fname) as im:
return im.asarray(*args, **kwargs)
self.imread = imread
self.pattern = self._axes_pattern if pattern == 'axes' else pattern
try:
self._parse()
if not self.axes:
self.axes = 'I'
except self._ParseError:
self.axes = 'I'
self.shape = (len(files),)
self._start_index = (0,)
self._indices = ((i,) for i in range(len(files)))
def __str__(self):
"""Return string with information about image sequence."""
return "\n".join([
self.files[0],
'* files: %i' % len(self.files),
'* axes: %s' % self.axes,
'* shape: %s' % str(self.shape)])
def __len__(self):
return len(self.files)
def __enter__(self):
return self
def __exit__(self, exc_type, exc_value, traceback):
self.close()
def close(self):
pass
def asarray(self, *args, **kwargs):
"""Read image data from all files and return as single numpy array.
Raise IndexError if image shapes don't match.
"""
im = self.imread(self.files[0])
result_shape = self.shape + im.shape
result = numpy.zeros(result_shape, dtype=im.dtype)
result = result.reshape(-1, *im.shape)
for index, fname in zip(self._indices, self.files):
index = [i-j for i, j in zip(index, self._start_index)]
index = numpy.ravel_multi_index(index, self.shape)
im = self.imread(fname, *args, **kwargs)
result[index] = im
result.shape = result_shape
return result
def _parse(self):
"""Get axes and shape from file names."""
if not self.pattern:
raise self._ParseError("invalid pattern")
pattern = re.compile(self.pattern, re.IGNORECASE | re.VERBOSE)
matches = pattern.findall(self.files[0])
if not matches:
raise self._ParseError("pattern doesn't match file names")
matches = matches[-1]
if len(matches) % 2:
raise self._ParseError("pattern doesn't match axis name and index")
axes = ''.join(m for m in matches[::2] if m)
if not axes:
raise self._ParseError("pattern doesn't match file names")
indices = []
for fname in self.files:
matches = pattern.findall(fname)[-1]
if axes != ''.join(m for m in matches[::2] if m):
raise ValueError("axes don't match within the image sequence")
indices.append([int(m) for m in matches[1::2] if m])
shape = tuple(numpy.max(indices, axis=0))
start_index = tuple(numpy.min(indices, axis=0))
shape = tuple(i-j+1 for i, j in zip(shape, start_index))
if numpy.prod(shape) != len(self.files):
warnings.warn("files are missing. Missing data are zeroed")
self.axes = axes.upper()
self.shape = shape
self._indices = indices
self._start_index = start_index
class Record(dict):
"""Dictionary with attribute access.
Can also be initialized with numpy.core.records.record.
"""
__slots__ = ()
def __init__(self, arg=None, **kwargs):
if kwargs:
arg = kwargs
elif arg is None:
arg = {}
try:
dict.__init__(self, arg)
except (TypeError, ValueError):
for i, name in enumerate(arg.dtype.names):
v = arg[i]
self[name] = v if v.dtype.char != 'S' else stripnull(v)
def __getattr__(self, name):
return self[name]
def __setattr__(self, name, value):
self.__setitem__(name, value)
def __str__(self):
"""Pretty print Record."""
s = []
lists = []
for k in sorted(self):
if k.startswith('_'): # does not work with byte
continue
v = self[k]
if isinstance(v, (list, tuple)) and len(v):
if isinstance(v[0], Record):
lists.append((k, v))
continue
elif isinstance(v[0], TiffPage):
v = [i.index for i in v if i]
s.append(
("* %s: %s" % (k, str(v))).split("\n", 1)[0]
[:PRINT_LINE_LEN].rstrip())
for k, v in lists:
l = []
for i, w in enumerate(v):
l.append("* %s[%i]\n %s" % (k, i,
str(w).replace("\n", "\n ")))
s.append('\n'.join(l))
return '\n'.join(s)
class TiffTags(Record):
"""Dictionary of TiffTags with attribute access."""
def __str__(self):
"""Return string with information about all tags."""
s = []
for tag in sorted(self.values(), key=lambda x: x.code):
typecode = "%i%s" % (tag.count * int(tag.dtype[0]), tag.dtype[1])
line = "* %i %s (%s) %s" % (tag.code, tag.name, typecode,
str(tag.value).split('\n', 1)[0])
s.append(line[:PRINT_LINE_LEN].lstrip())
return '\n'.join(s)
def read_bytes(fh, byteorder, dtype, count):
"""Read tag data from file and return as byte string."""
return numpy_fromfile(fh, byteorder+dtype[-1], count).tostring()
def read_numpy(fh, byteorder, dtype, count):
"""Read tag data from file and return as numpy array."""
return numpy_fromfile(fh, byteorder+dtype[-1], count)
def read_json(fh, byteorder, dtype, count):
"""Read tag data from file and return as object."""
return json.loads(unicode(stripnull(fh.read(count)), 'utf-8'))
def read_mm_header(fh, byteorder, dtype, count):
"""Read MM_HEADER tag from file and return as numpy.rec.array."""
return numpy.rec.fromfile(fh, MM_HEADER, 1, byteorder=byteorder)[0]
def read_mm_stamp(fh, byteorder, dtype, count):
"""Read MM_STAMP tag from file and return as numpy.array."""
return numpy_fromfile(fh, byteorder+'8f8', 1)[0]
def read_mm_uic1(fh, byteorder, dtype, count):
"""Read MM_UIC1 tag from file and return as dictionary."""
t = fh.read(8*count)
t = struct.unpack('%s%iI' % (byteorder, 2*count), t)
return dict((MM_TAG_IDS[k], v) for k, v in zip(t[::2], t[1::2])
if k in MM_TAG_IDS)
def read_mm_uic2(fh, byteorder, dtype, count):
"""Read MM_UIC2 tag from file and return as dictionary."""
result = {'number_planes': count}
values = numpy_fromfile(fh, byteorder+'I', 6*count)
result['z_distance'] = values[0::6] // values[1::6]
#result['date_created'] = tuple(values[2::6])
#result['time_created'] = tuple(values[3::6])
#result['date_modified'] = tuple(values[4::6])
#result['time_modified'] = tuple(values[5::6])
return result
def read_mm_uic3(fh, byteorder, dtype, count):
"""Read MM_UIC3 tag from file and return as dictionary."""
t = numpy_fromfile(fh, byteorder+'I', 2*count)
return {'wavelengths': t[0::2] // t[1::2]}
def read_mm_uic4(fh, byteorder, dtype, count):
"""Read MM_UIC4 tag from file and return as dictionary."""
t = struct.unpack(byteorder + 'hI'*count, fh.read(6*count))
return dict((MM_TAG_IDS[k], v) for k, v in zip(t[::2], t[1::2])
if k in MM_TAG_IDS)
def read_cz_lsm_info(fh, byteorder, dtype, count):
"""Read CS_LSM_INFO tag from file and return as numpy.rec.array."""
result = numpy.rec.fromfile(fh, CZ_LSM_INFO, 1,
byteorder=byteorder)[0]
{50350412: '1.3', 67127628: '2.0'}[result.magic_number] # validation
return result
def read_cz_lsm_time_stamps(fh, byteorder):
"""Read LSM time stamps from file and return as list."""
size, count = struct.unpack(byteorder+'II', fh.read(8))
if size != (8 + 8 * count):
raise ValueError("lsm_time_stamps block is too short")
return struct.unpack(('%s%dd' % (byteorder, count)),
fh.read(8*count))
def read_cz_lsm_event_list(fh, byteorder):
"""Read LSM events from file and return as list of (time, type, text)."""
count = struct.unpack(byteorder+'II', fh.read(8))[1]
events = []
while count > 0:
esize, etime, etype = struct.unpack(byteorder+'IdI', fh.read(16))
etext = stripnull(fh.read(esize - 16))
events.append((etime, etype, etext))
count -= 1
return events
def read_cz_lsm_scan_info(fh, byteorder):
"""Read LSM scan information from file and return as Record."""
block = Record()
blocks = [block]
unpack = struct.unpack
if 0x10000000 != struct.unpack(byteorder+"I", fh.read(4))[0]:
raise ValueError("not a lsm_scan_info structure")
fh.read(8)
while True:
entry, dtype, size = unpack(byteorder+"III", fh.read(12))
if dtype == 2:
value = stripnull(fh.read(size))
elif dtype == 4:
value = unpack(byteorder+"i", fh.read(4))[0]
elif dtype == 5:
value = unpack(byteorder+"d", fh.read(8))[0]
else:
value = 0
if entry in CZ_LSM_SCAN_INFO_ARRAYS:
blocks.append(block)
name = CZ_LSM_SCAN_INFO_ARRAYS[entry]
newobj = []
setattr(block, name, newobj)
block = newobj
elif entry in CZ_LSM_SCAN_INFO_STRUCTS:
blocks.append(block)
newobj = Record()
block.append(newobj)
block = newobj
elif entry in CZ_LSM_SCAN_INFO_ATTRIBUTES:
name = CZ_LSM_SCAN_INFO_ATTRIBUTES[entry]
setattr(block, name, value)
elif entry == 0xffffffff:
block = blocks.pop()
else:
setattr(block, "unknown_%x" % entry, value)
if not blocks:
break
return block
def read_nih_image_header(fh, byteorder, dtype, count):
"""Read NIH_IMAGE_HEADER tag from file and return as numpy.rec.array."""
a = numpy.rec.fromfile(fh, NIH_IMAGE_HEADER, 1, byteorder=byteorder)[0]
a = a.newbyteorder(byteorder)
a.xunit = a.xunit[:a._xunit_len]
a.um = a.um[:a._um_len]
return a
def imagej_metadata(data, bytecounts, byteorder):
"""Return dict from ImageJ meta data tag value."""
_str = str if sys.version_info[0] < 3 else lambda x: str(x, 'cp1252')
def read_string(data, byteorder):
return _str(stripnull(data[0 if byteorder == '<' else 1::2]))
def read_double(data, byteorder):
return struct.unpack(byteorder+('d' * (len(data) // 8)), data)
def read_bytes(data, byteorder):
#return struct.unpack('b' * len(data), data)
return numpy.fromstring(data, 'uint8')
metadata_types = { # big endian
b'info': ('info', read_string),
b'labl': ('labels', read_string),
b'rang': ('ranges', read_double),
b'luts': ('luts', read_bytes),
b'roi ': ('roi', read_bytes),
b'over': ('overlays', read_bytes)}
metadata_types.update( # little endian
dict((k[::-1], v) for k, v in metadata_types.items()))
if not bytecounts:
raise ValueError("no ImageJ meta data")
if not data[:4] in (b'IJIJ', b'JIJI'):
raise ValueError("invalid ImageJ meta data")
header_size = bytecounts[0]
if header_size < 12 or header_size > 804:
raise ValueError("invalid ImageJ meta data header size")
ntypes = (header_size - 4) // 8
header = struct.unpack(byteorder+'4sI'*ntypes, data[4:4+ntypes*8])
pos = 4 + ntypes * 8
counter = 0
result = {}
for mtype, count in zip(header[::2], header[1::2]):
values = []
name, func = metadata_types.get(mtype, (_str(mtype), read_bytes))
for _ in range(count):
counter += 1
pos1 = pos + bytecounts[counter]
values.append(func(data[pos:pos1], byteorder))
pos = pos1
result[name.strip()] = values[0] if count == 1 else values
return result
def imagej_description(description):
"""Return dict from ImageJ image_description tag."""
def _bool(val):
return {b'true': True, b'false': False}[val.lower()]
_str = str if sys.version_info[0] < 3 else lambda x: str(x, 'cp1252')
result = {}
for line in description.splitlines():
try:
key, val = line.split(b'=')
except Exception:
continue
key = key.strip()
val = val.strip()
for dtype in (int, float, _bool, _str):
try:
val = dtype(val)
break
except Exception:
pass
result[_str(key)] = val
return result
def read_micromanager_metadata(fh):
"""Read MicroManager non-TIFF settings from open file and return as dict.
The settings can be used to read image data without parsing the TIFF file.
Raise ValueError if file does not contain valid MicroManager metadata.
"""
fh.seek(0)
try:
byteorder = {b'II': '<', b'MM': '>'}[fh.read(2)]
except IndexError:
raise ValueError("not a MicroManager TIFF file")
results = {}
fh.seek(8)
(index_header, index_offset, display_header, display_offset,
comments_header, comments_offset, summary_header, summary_length
) = struct.unpack(byteorder + "IIIIIIII", fh.read(32))
if summary_header != 2355492:
raise ValueError("invalid MicroManager summary_header")
results['summary'] = read_json(fh, byteorder, None, summary_length)
if index_header != 54773648:
raise ValueError("invalid MicroManager index_header")
fh.seek(index_offset)
header, count = struct.unpack(byteorder + "II", fh.read(8))
if header != 3453623:
raise ValueError("invalid MicroManager index_header")
data = struct.unpack(byteorder + "IIIII"*count, fh.read(20*count))
results['index_map'] = {
'channel': data[::5], 'slice': data[1::5], 'frame': data[2::5],
'position': data[3::5], 'offset': data[4::5]}
if display_header != 483765892:
raise ValueError("invalid MicroManager display_header")
fh.seek(display_offset)
header, count = struct.unpack(byteorder + "II", fh.read(8))
if header != 347834724:
raise ValueError("invalid MicroManager display_header")
results['display_settings'] = read_json(fh, byteorder, None, count)
if comments_header != 99384722:
raise ValueError("invalid MicroManager comments_header")
fh.seek(comments_offset)
header, count = struct.unpack(byteorder + "II", fh.read(8))
if header != 84720485:
raise ValueError("invalid MicroManager comments_header")
results['comments'] = read_json(fh, byteorder, None, count)
return results
def _replace_by(module_function, package=None, warn=True):
"""Try replace decorated function by module.function."""
try:
from importlib import import_module
except ImportError:
warnings.warn('Could not import module importlib')
return lambda func: func
def decorate(func, module_function=module_function, warn=warn):
try:
module, function = module_function.split('.')
if not package:
module = import_module(module)
else:
module = import_module('.' + module, package=package)
func, oldfunc = getattr(module, function), func
globals()['__old_' + func.__name__] = oldfunc
except Exception:
if warn:
warnings.warn("failed to import %s" % module_function)
return func
return decorate
@_replace_by('_tifffile.decodepackbits')
def decodepackbits(encoded):
"""Decompress PackBits encoded byte string.
PackBits is a simple byte-oriented run-length compression scheme.
"""
func = ord if sys.version[0] == '2' else lambda x: x
result = []
result_extend = result.extend
i = 0
try:
while True:
n = func(encoded[i]) + 1
i += 1
if n < 129:
result_extend(encoded[i:i+n])
i += n
elif n > 129:
result_extend(encoded[i:i+1] * (258-n))
i += 1
except IndexError:
pass
return b''.join(result) if sys.version[0] == '2' else bytes(result)
@_replace_by('_tifffile.decodelzw')
def decodelzw(encoded):
"""Decompress LZW (Lempel-Ziv-Welch) encoded TIFF strip (byte string).
The strip must begin with a CLEAR code and end with an EOI code.
This is an implementation of the LZW decoding algorithm described in (1).
It is not compatible with old style LZW compressed files like quad-lzw.tif.
"""
len_encoded = len(encoded)
bitcount_max = len_encoded * 8
unpack = struct.unpack
if sys.version[0] == '2':
newtable = [chr(i) for i in range(256)]
else:
newtable = [bytes([i]) for i in range(256)]
newtable.extend((0, 0))
def next_code():
"""Return integer of `bitw` bits at `bitcount` position in encoded."""
start = bitcount // 8
s = encoded[start:start+4]
try:
code = unpack('>I', s)[0]
except Exception:
code = unpack('>I', s + b'\x00'*(4-len(s)))[0]
code <<= bitcount % 8
code &= mask
return code >> shr
switchbitch = { # code: bit-width, shr-bits, bit-mask
255: (9, 23, int(9*'1'+'0'*23, 2)),
511: (10, 22, int(10*'1'+'0'*22, 2)),
1023: (11, 21, int(11*'1'+'0'*21, 2)),
2047: (12, 20, int(12*'1'+'0'*20, 2)), }
bitw, shr, mask = switchbitch[255]
bitcount = 0
if len_encoded < 4:
raise ValueError("strip must be at least 4 characters long")
if next_code() != 256:
raise ValueError("strip must begin with CLEAR code")
code = 0
oldcode = 0
result = []
result_append = result.append
while True:
code = next_code() # ~5% faster when inlining this function
bitcount += bitw
if code == 257 or bitcount >= bitcount_max: # EOI
break
if code == 256: # CLEAR
table = newtable[:]
table_append = table.append
lentable = 258
bitw, shr, mask = switchbitch[255]
code = next_code()
bitcount += bitw
if code == 257: # EOI
break
result_append(table[code])
else:
if code < lentable:
decoded = table[code]
newcode = table[oldcode] + decoded[:1]
else:
newcode = table[oldcode]
newcode += newcode[:1]
decoded = newcode
result_append(decoded)
table_append(newcode)
lentable += 1
oldcode = code
if lentable in switchbitch:
bitw, shr, mask = switchbitch[lentable]
if code != 257:
warnings.warn(
"decodelzw encountered unexpected end of stream (code %i)" % code)
return b''.join(result)
@_replace_by('_tifffile.unpackints')
def unpackints(data, dtype, itemsize, runlen=0):
"""Decompress byte string to array of integers of any bit size <= 32.
Parameters
----------
data : byte str
Data to decompress.
dtype : numpy.dtype or str
A numpy boolean or integer type.
itemsize : int
Number of bits per integer.
runlen : int
Number of consecutive integers, after which to start at next byte.
"""
if itemsize == 1: # bitarray
data = numpy.fromstring(data, '|B')
data = numpy.unpackbits(data)
if runlen % 8:
data = data.reshape(-1, runlen + (8 - runlen % 8))
data = data[:, :runlen].reshape(-1)
return data.astype(dtype)
dtype = numpy.dtype(dtype)
if itemsize in (8, 16, 32, 64):
return numpy.fromstring(data, dtype)
if itemsize < 1 or itemsize > 32:
raise ValueError("itemsize out of range: %i" % itemsize)
if dtype.kind not in "biu":
raise ValueError("invalid dtype")
itembytes = next(i for i in (1, 2, 4, 8) if 8 * i >= itemsize)
if itembytes != dtype.itemsize:
raise ValueError("dtype.itemsize too small")
if runlen == 0:
runlen = len(data) // itembytes
skipbits = runlen*itemsize % 8
if skipbits:
skipbits = 8 - skipbits
shrbits = itembytes*8 - itemsize
bitmask = int(itemsize*'1'+'0'*shrbits, 2)
dtypestr = '>' + dtype.char # dtype always big endian?
unpack = struct.unpack
l = runlen * (len(data)*8 // (runlen*itemsize + skipbits))
result = numpy.empty((l, ), dtype)
bitcount = 0
for i in range(len(result)):
start = bitcount // 8
s = data[start:start+itembytes]
try:
code = unpack(dtypestr, s)[0]
except Exception:
code = unpack(dtypestr, s + b'\x00'*(itembytes-len(s)))[0]
code <<= bitcount % 8
code &= bitmask
result[i] = code >> shrbits
bitcount += itemsize
if (i+1) % runlen == 0:
bitcount += skipbits
return result
def unpackrgb(data, dtype='<B', bitspersample=(5, 6, 5), rescale=True):
"""Return array from byte string containing packed samples.
Use to unpack RGB565 or RGB555 to RGB888 format.
Parameters
----------
data : byte str
The data to be decoded. Samples in each pixel are stored consecutively.
Pixels are aligned to 8, 16, or 32 bit boundaries.
dtype : numpy.dtype
The sample data type. The byteorder applies also to the data stream.
bitspersample : tuple
Number of bits for each sample in a pixel.
rescale : bool
Upscale samples to the number of bits in dtype.
Returns
-------
result : ndarray
Flattened array of unpacked samples of native dtype.
Examples
--------
>>> data = struct.pack('BBBB', 0x21, 0x08, 0xff, 0xff)
>>> print(unpackrgb(data, '<B', (5, 6, 5), False))
[ 1 1 1 31 63 31]
>>> print(unpackrgb(data, '<B', (5, 6, 5)))
[ 8 4 8 255 255 255]
>>> print(unpackrgb(data, '<B', (5, 5, 5)))
[ 16 8 8 255 255 255]
"""
dtype = numpy.dtype(dtype)
bits = int(numpy.sum(bitspersample))
if not (bits <= 32 and all(i <= dtype.itemsize*8 for i in bitspersample)):
raise ValueError("sample size not supported %s" % str(bitspersample))
dt = next(i for i in 'BHI' if numpy.dtype(i).itemsize*8 >= bits)
data = numpy.fromstring(data, dtype.byteorder+dt)
result = numpy.empty((data.size, len(bitspersample)), dtype.char)
for i, bps in enumerate(bitspersample):
t = data >> int(numpy.sum(bitspersample[i+1:]))
t &= int('0b'+'1'*bps, 2)
if rescale:
o = ((dtype.itemsize * 8) // bps + 1) * bps
if o > data.dtype.itemsize * 8:
t = t.astype('I')
t *= (2**o - 1) // (2**bps - 1)
t //= 2**(o - (dtype.itemsize * 8))
result[:, i] = t
return result.reshape(-1)
def reorient(image, orientation):
"""Return reoriented view of image array.
Parameters
----------
image : numpy array
Non-squeezed output of asarray() functions.
Axes -3 and -2 must be image length and width respectively.
orientation : int or str
One of TIFF_ORIENTATIONS keys or values.
"""
o = TIFF_ORIENTATIONS.get(orientation, orientation)
if o == 'top_left':
return image
elif o == 'top_right':
return image[..., ::-1, :]
elif o == 'bottom_left':
return image[..., ::-1, :, :]
elif o == 'bottom_right':
return image[..., ::-1, ::-1, :]
elif o == 'left_top':
return numpy.swapaxes(image, -3, -2)
elif o == 'right_top':
return numpy.swapaxes(image, -3, -2)[..., ::-1, :]
elif o == 'left_bottom':
return numpy.swapaxes(image, -3, -2)[..., ::-1, :, :]
elif o == 'right_bottom':
return numpy.swapaxes(image, -3, -2)[..., ::-1, ::-1, :]
def numpy_fromfile(arg, dtype=float, count=-1, sep=''):
"""Return array from data in binary file.
Work around numpy issue #2230, "numpy.fromfile does not accept StringIO
object" https://github.com/numpy/numpy/issues/2230.
"""
try:
return numpy.fromfile(arg, dtype, count, sep)
except IOError:
if count < 0:
size = 2**30
else:
size = count * numpy.dtype(dtype).itemsize
data = arg.read(int(size))
return numpy.fromstring(data, dtype, count, sep)
def stripnull(string):
"""Return string truncated at first null character."""
i = string.find(b'\x00')
return string if (i < 0) else string[:i]
def format_size(size):
"""Return file size as string from byte size."""
for unit in ('B', 'KB', 'MB', 'GB', 'TB'):
if size < 2048:
return "%.f %s" % (size, unit)
size /= 1024.0
def natural_sorted(iterable):
"""Return human sorted list of strings.
>>> natural_sorted(['f1', 'f2', 'f10'])
['f1', 'f2', 'f10']
"""
def sortkey(x):
return [(int(c) if c.isdigit() else c) for c in re.split(numbers, x)]
numbers = re.compile('(\d+)')
return sorted(iterable, key=sortkey)
def datetime_from_timestamp(n, epoch=datetime.datetime.fromordinal(693594)):
"""Return datetime object from timestamp in Excel serial format.
Examples
--------
>>> datetime_from_timestamp(40237.029999999795)
datetime.datetime(2010, 2, 28, 0, 43, 11, 999982)
"""
return epoch + datetime.timedelta(n)
def test_tifffile(directory='testimages', verbose=True):
"""Read all images in directory. Print error message on failure.
Examples
--------
>>> test_tifffile(verbose=False)
"""
successful = 0
failed = 0
start = time.time()
for f in glob.glob(os.path.join(directory, '*.*')):
if verbose:
print("\n%s>\n" % f.lower(), end='')
t0 = time.time()
try:
tif = TiffFile(f, multifile=True)
except Exception as e:
if not verbose:
print(f, end=' ')
print("ERROR:", e)
failed += 1
continue
try:
img = tif.asarray()
except ValueError:
try:
img = tif[0].asarray()
except Exception as e:
if not verbose:
print(f, end=' ')
print("ERROR:", e)
failed += 1
continue
finally:
tif.close()
successful += 1
if verbose:
print("%s, %s %s, %s, %.0f ms" % (
str(tif), str(img.shape), img.dtype, tif[0].compression,
(time.time()-t0) * 1e3))
if verbose:
print("\nSuccessfully read %i of %i files in %.3f s\n" % (
successful, successful+failed, time.time()-start))
class TIFF_SUBFILE_TYPES(object):
def __getitem__(self, key):
result = []
if key & 1:
result.append('reduced_image')
if key & 2:
result.append('page')
if key & 4:
result.append('mask')
return tuple(result)
TIFF_PHOTOMETRICS = {
0: 'miniswhite',
1: 'minisblack',
2: 'rgb',
3: 'palette',
4: 'mask',
5: 'separated',
6: 'cielab',
7: 'icclab',
8: 'itulab',
32844: 'logl',
32845: 'logluv',
}
TIFF_COMPESSIONS = {
1: None,
2: 'ccittrle',
3: 'ccittfax3',
4: 'ccittfax4',
5: 'lzw',
6: 'ojpeg',
7: 'jpeg',
8: 'adobe_deflate',
9: 't85',
10: 't43',
32766: 'next',
32771: 'ccittrlew',
32773: 'packbits',
32809: 'thunderscan',
32895: 'it8ctpad',
32896: 'it8lw',
32897: 'it8mp',
32898: 'it8bl',
32908: 'pixarfilm',
32909: 'pixarlog',
32946: 'deflate',
32947: 'dcs',
34661: 'jbig',
34676: 'sgilog',
34677: 'sgilog24',
34712: 'jp2000',
34713: 'nef',
}
TIFF_DECOMPESSORS = {
None: lambda x: x,
'adobe_deflate': zlib.decompress,
'deflate': zlib.decompress,
'packbits': decodepackbits,
'lzw': decodelzw,
}
TIFF_DATA_TYPES = {
1: '1B', # BYTE 8-bit unsigned integer.
2: '1s', # ASCII 8-bit byte that contains a 7-bit ASCII code;
# the last byte must be NULL (binary zero).
3: '1H', # SHORT 16-bit (2-byte) unsigned integer
4: '1I', # LONG 32-bit (4-byte) unsigned integer.
5: '2I', # RATIONAL Two LONGs: the first represents the numerator of
# a fraction; the second, the denominator.
6: '1b', # SBYTE An 8-bit signed (twos-complement) integer.
7: '1B', # UNDEFINED An 8-bit byte that may contain anything,
# depending on the definition of the field.
8: '1h', # SSHORT A 16-bit (2-byte) signed (twos-complement) integer.
9: '1i', # SLONG A 32-bit (4-byte) signed (twos-complement) integer.
10: '2i', # SRATIONAL Two SLONGs: the first represents the numerator
# of a fraction, the second the denominator.
11: '1f', # FLOAT Single precision (4-byte) IEEE format.
12: '1d', # DOUBLE Double precision (8-byte) IEEE format.
13: '1I', # IFD unsigned 4 byte IFD offset.
#14: '', # UNICODE
#15: '', # COMPLEX
16: '1Q', # LONG8 unsigned 8 byte integer (BigTiff)
17: '1q', # SLONG8 signed 8 byte integer (BigTiff)
18: '1Q', # IFD8 unsigned 8 byte IFD offset (BigTiff)
}
TIFF_SAMPLE_FORMATS = {
1: 'uint',
2: 'int',
3: 'float',
#4: 'void',
#5: 'complex_int',
6: 'complex',
}
TIFF_SAMPLE_DTYPES = {
('uint', 1): '?', # bitmap
('uint', 2): 'B',
('uint', 3): 'B',
('uint', 4): 'B',
('uint', 5): 'B',
('uint', 6): 'B',
('uint', 7): 'B',
('uint', 8): 'B',
('uint', 9): 'H',
('uint', 10): 'H',
('uint', 11): 'H',
('uint', 12): 'H',
('uint', 13): 'H',
('uint', 14): 'H',
('uint', 15): 'H',
('uint', 16): 'H',
('uint', 17): 'I',
('uint', 18): 'I',
('uint', 19): 'I',
('uint', 20): 'I',
('uint', 21): 'I',
('uint', 22): 'I',
('uint', 23): 'I',
('uint', 24): 'I',
('uint', 25): 'I',
('uint', 26): 'I',
('uint', 27): 'I',
('uint', 28): 'I',
('uint', 29): 'I',
('uint', 30): 'I',
('uint', 31): 'I',
('uint', 32): 'I',
('uint', 64): 'Q',
('int', 8): 'b',
('int', 16): 'h',
('int', 32): 'i',
('int', 64): 'q',
('float', 16): 'e',
('float', 32): 'f',
('float', 64): 'd',
('complex', 64): 'F',
('complex', 128): 'D',
('uint', (5, 6, 5)): 'B',
}
TIFF_ORIENTATIONS = {
1: 'top_left',
2: 'top_right',
3: 'bottom_right',
4: 'bottom_left',
5: 'left_top',
6: 'right_top',
7: 'right_bottom',
8: 'left_bottom',
}
AXES_LABELS = {
'X': 'width',
'Y': 'height',
'Z': 'depth',
'S': 'sample', # rgb(a)
'P': 'plane', # page
'T': 'time',
'C': 'channel', # color, emission wavelength
'A': 'angle',
'F': 'phase',
'R': 'tile', # region, point
'H': 'lifetime', # histogram
'E': 'lambda', # excitation wavelength
'L': 'exposure', # lux
'V': 'event',
'Q': 'other',
}
AXES_LABELS.update(dict((v, k) for k, v in AXES_LABELS.items()))
# NIH Image PicHeader v1.63
NIH_IMAGE_HEADER = [
('fileid', 'a8'),
('nlines', 'i2'),
('pixelsperline', 'i2'),
('version', 'i2'),
('oldlutmode', 'i2'),
('oldncolors', 'i2'),
('colors', 'u1', (3, 32)),
('oldcolorstart', 'i2'),
('colorwidth', 'i2'),
('extracolors', 'u2', (6, 3)),
('nextracolors', 'i2'),
('foregroundindex', 'i2'),
('backgroundindex', 'i2'),
('xscale', 'f8'),
('_x0', 'i2'),
('_x1', 'i2'),
('units_t', 'i2'),
('p1', [('x', 'i2'), ('y', 'i2')]),
('p2', [('x', 'i2'), ('y', 'i2')]),
('curvefit_t', 'i2'),
('ncoefficients', 'i2'),
('coeff', 'f8', 6),
('_um_len', 'u1'),
('um', 'a15'),
('_x2', 'u1'),
('binarypic', 'b1'),
('slicestart', 'i2'),
('sliceend', 'i2'),
('scalemagnification', 'f4'),
('nslices', 'i2'),
('slicespacing', 'f4'),
('currentslice', 'i2'),
('frameinterval', 'f4'),
('pixelaspectratio', 'f4'),
('colorstart', 'i2'),
('colorend', 'i2'),
('ncolors', 'i2'),
('fill1', '3u2'),
('fill2', '3u2'),
('colortable_t', 'u1'),
('lutmode_t', 'u1'),
('invertedtable', 'b1'),
('zeroclip', 'b1'),
('_xunit_len', 'u1'),
('xunit', 'a11'),
('stacktype_t', 'i2'),
]
#NIH_COLORTABLE_TYPE = (
# 'CustomTable', 'AppleDefault', 'Pseudo20', 'Pseudo32', 'Rainbow',
# 'Fire1', 'Fire2', 'Ice', 'Grays', 'Spectrum')
#NIH_LUTMODE_TYPE = (
# 'PseudoColor', 'OldAppleDefault', 'OldSpectrum', 'GrayScale',
# 'ColorLut', 'CustomGrayscale')
#NIH_CURVEFIT_TYPE = (
# 'StraightLine', 'Poly2', 'Poly3', 'Poly4', 'Poly5', 'ExpoFit',
# 'PowerFit', 'LogFit', 'RodbardFit', 'SpareFit1', 'Uncalibrated',
# 'UncalibratedOD')
#NIH_UNITS_TYPE = (
# 'Nanometers', 'Micrometers', 'Millimeters', 'Centimeters', 'Meters',
# 'Kilometers', 'Inches', 'Feet', 'Miles', 'Pixels', 'OtherUnits')
#NIH_STACKTYPE_TYPE = (
# 'VolumeStack', 'RGBStack', 'MovieStack', 'HSVStack')
# MetaMorph STK tags
MM_TAG_IDS = {
0: 'auto_scale',
1: 'min_scale',
2: 'max_scale',
3: 'spatial_calibration',
#4: 'x_calibration',
#5: 'y_calibration',
#6: 'calibration_units',
#7: 'name',
8: 'thresh_state',
9: 'thresh_state_red',
11: 'thresh_state_green',
12: 'thresh_state_blue',
13: 'thresh_state_lo',
14: 'thresh_state_hi',
15: 'zoom',
#16: 'create_time',
#17: 'last_saved_time',
18: 'current_buffer',
19: 'gray_fit',
20: 'gray_point_count',
#21: 'gray_x',
#22: 'gray_y',
#23: 'gray_min',
#24: 'gray_max',
#25: 'gray_unit_name',
26: 'standard_lut',
27: 'wavelength',
#28: 'stage_position',
#29: 'camera_chip_offset',
#30: 'overlay_mask',
#31: 'overlay_compress',
#32: 'overlay',
#33: 'special_overlay_mask',
#34: 'special_overlay_compress',
#35: 'special_overlay',
36: 'image_property',
#37: 'stage_label',
#38: 'autoscale_lo_info',
#39: 'autoscale_hi_info',
#40: 'absolute_z',
#41: 'absolute_z_valid',
#42: 'gamma',
#43: 'gamma_red',
#44: 'gamma_green',
#45: 'gamma_blue',
#46: 'camera_bin',
47: 'new_lut',
#48: 'image_property_ex',
49: 'plane_property',
#50: 'user_lut_table',
51: 'red_autoscale_info',
#52: 'red_autoscale_lo_info',
#53: 'red_autoscale_hi_info',
54: 'red_minscale_info',
55: 'red_maxscale_info',
56: 'green_autoscale_info',
#57: 'green_autoscale_lo_info',
#58: 'green_autoscale_hi_info',
59: 'green_minscale_info',
60: 'green_maxscale_info',
61: 'blue_autoscale_info',
#62: 'blue_autoscale_lo_info',
#63: 'blue_autoscale_hi_info',
64: 'blue_min_scale_info',
65: 'blue_max_scale_info',
#66: 'overlay_plane_color'
}
# Olympus FluoView
MM_DIMENSION = [
('name', 'a16'),
('size', 'i4'),
('origin', 'f8'),
('resolution', 'f8'),
('unit', 'a64'),
]
MM_HEADER = [
('header_flag', 'i2'),
('image_type', 'u1'),
('image_name', 'a257'),
('offset_data', 'u4'),
('palette_size', 'i4'),
('offset_palette0', 'u4'),
('offset_palette1', 'u4'),
('comment_size', 'i4'),
('offset_comment', 'u4'),
('dimensions', MM_DIMENSION, 10),
('offset_position', 'u4'),
('map_type', 'i2'),
('map_min', 'f8'),
('map_max', 'f8'),
('min_value', 'f8'),
('max_value', 'f8'),
('offset_map', 'u4'),
('gamma', 'f8'),
('offset', 'f8'),
('gray_channel', MM_DIMENSION),
('offset_thumbnail', 'u4'),
('voice_field', 'i4'),
('offset_voice_field', 'u4'),
]
# Carl Zeiss LSM
CZ_LSM_INFO = [
('magic_number', 'i4'),
('structure_size', 'i4'),
('dimension_x', 'i4'),
('dimension_y', 'i4'),
('dimension_z', 'i4'),
('dimension_channels', 'i4'),
('dimension_time', 'i4'),
('dimension_data_type', 'i4'),
('thumbnail_x', 'i4'),
('thumbnail_y', 'i4'),
('voxel_size_x', 'f8'),
('voxel_size_y', 'f8'),
('voxel_size_z', 'f8'),
('origin_x', 'f8'),
('origin_y', 'f8'),
('origin_z', 'f8'),
('scan_type', 'u2'),
('spectral_scan', 'u2'),
('data_type', 'u4'),
('offset_vector_overlay', 'u4'),
('offset_input_lut', 'u4'),
('offset_output_lut', 'u4'),
('offset_channel_colors', 'u4'),
('time_interval', 'f8'),
('offset_channel_data_types', 'u4'),
('offset_scan_information', 'u4'),
('offset_ks_data', 'u4'),
('offset_time_stamps', 'u4'),
('offset_event_list', 'u4'),
('offset_roi', 'u4'),
('offset_bleach_roi', 'u4'),
('offset_next_recording', 'u4'),
('display_aspect_x', 'f8'),
('display_aspect_y', 'f8'),
('display_aspect_z', 'f8'),
('display_aspect_time', 'f8'),
('offset_mean_of_roi_overlay', 'u4'),
('offset_topo_isoline_overlay', 'u4'),
('offset_topo_profile_overlay', 'u4'),
('offset_linescan_overlay', 'u4'),
('offset_toolbar_flags', 'u4'),
]
# Import functions for LSM_INFO sub-records
CZ_LSM_INFO_READERS = {
'scan_information': read_cz_lsm_scan_info,
'time_stamps': read_cz_lsm_time_stamps,
'event_list': read_cz_lsm_event_list,
}
# Map cz_lsm_info.scan_type to dimension order
CZ_SCAN_TYPES = {
0: 'XYZCT', # x-y-z scan
1: 'XYZCT', # z scan (x-z plane)
2: 'XYZCT', # line scan
3: 'XYTCZ', # time series x-y
4: 'XYZTC', # time series x-z
5: 'XYTCZ', # time series 'Mean of ROIs'
6: 'XYZTC', # time series x-y-z
7: 'XYCTZ', # spline scan
8: 'XYCZT', # spline scan x-z
9: 'XYTCZ', # time series spline plane x-z
10: 'XYZCT', # point mode
}
# Map dimension codes to cz_lsm_info attribute
CZ_DIMENSIONS = {
'X': 'dimension_x',
'Y': 'dimension_y',
'Z': 'dimension_z',
'C': 'dimension_channels',
'T': 'dimension_time',
}
# Descriptions of cz_lsm_info.data_type
CZ_DATA_TYPES = {
0: 'varying data types',
2: '12 bit unsigned integer',
5: '32 bit float',
}
CZ_LSM_SCAN_INFO_ARRAYS = {
0x20000000: "tracks",
0x30000000: "lasers",
0x60000000: "detectionchannels",
0x80000000: "illuminationchannels",
0xa0000000: "beamsplitters",
0xc0000000: "datachannels",
0x13000000: "markers",
0x11000000: "timers",
}
CZ_LSM_SCAN_INFO_STRUCTS = {
0x40000000: "tracks",
0x50000000: "lasers",
0x70000000: "detectionchannels",
0x90000000: "illuminationchannels",
0xb0000000: "beamsplitters",
0xd0000000: "datachannels",
0x14000000: "markers",
0x12000000: "timers",
}
CZ_LSM_SCAN_INFO_ATTRIBUTES = {
0x10000001: "name",
0x10000002: "description",
0x10000003: "notes",
0x10000004: "objective",
0x10000005: "processing_summary",
0x10000006: "special_scan_mode",
0x10000007: "oledb_recording_scan_type",
0x10000008: "oledb_recording_scan_mode",
0x10000009: "number_of_stacks",
0x1000000a: "lines_per_plane",
0x1000000b: "samples_per_line",
0x1000000c: "planes_per_volume",
0x1000000d: "images_width",
0x1000000e: "images_height",
0x1000000f: "images_number_planes",
0x10000010: "images_number_stacks",
0x10000011: "images_number_channels",
0x10000012: "linscan_xy_size",
0x10000013: "scan_direction",
0x10000014: "time_series",
0x10000015: "original_scan_data",
0x10000016: "zoom_x",
0x10000017: "zoom_y",
0x10000018: "zoom_z",
0x10000019: "sample_0x",
0x1000001a: "sample_0y",
0x1000001b: "sample_0z",
0x1000001c: "sample_spacing",
0x1000001d: "line_spacing",
0x1000001e: "plane_spacing",
0x1000001f: "plane_width",
0x10000020: "plane_height",
0x10000021: "volume_depth",
0x10000023: "nutation",
0x10000034: "rotation",
0x10000035: "precession",
0x10000036: "sample_0time",
0x10000037: "start_scan_trigger_in",
0x10000038: "start_scan_trigger_out",
0x10000039: "start_scan_event",
0x10000040: "start_scan_time",
0x10000041: "stop_scan_trigger_in",
0x10000042: "stop_scan_trigger_out",
0x10000043: "stop_scan_event",
0x10000044: "stop_scan_time",
0x10000045: "use_rois",
0x10000046: "use_reduced_memory_rois",
0x10000047: "user",
0x10000048: "use_bccorrection",
0x10000049: "position_bccorrection1",
0x10000050: "position_bccorrection2",
0x10000051: "interpolation_y",
0x10000052: "camera_binning",
0x10000053: "camera_supersampling",
0x10000054: "camera_frame_width",
0x10000055: "camera_frame_height",
0x10000056: "camera_offset_x",
0x10000057: "camera_offset_y",
# lasers
0x50000001: "name",
0x50000002: "acquire",
0x50000003: "power",
# tracks
0x40000001: "multiplex_type",
0x40000002: "multiplex_order",
0x40000003: "sampling_mode",
0x40000004: "sampling_method",
0x40000005: "sampling_number",
0x40000006: "acquire",
0x40000007: "sample_observation_time",
0x4000000b: "time_between_stacks",
0x4000000c: "name",
0x4000000d: "collimator1_name",
0x4000000e: "collimator1_position",
0x4000000f: "collimator2_name",
0x40000010: "collimator2_position",
0x40000011: "is_bleach_track",
0x40000012: "is_bleach_after_scan_number",
0x40000013: "bleach_scan_number",
0x40000014: "trigger_in",
0x40000015: "trigger_out",
0x40000016: "is_ratio_track",
0x40000017: "bleach_count",
0x40000018: "spi_center_wavelength",
0x40000019: "pixel_time",
0x40000021: "condensor_frontlens",
0x40000023: "field_stop_value",
0x40000024: "id_condensor_aperture",
0x40000025: "condensor_aperture",
0x40000026: "id_condensor_revolver",
0x40000027: "condensor_filter",
0x40000028: "id_transmission_filter1",
0x40000029: "id_transmission1",
0x40000030: "id_transmission_filter2",
0x40000031: "id_transmission2",
0x40000032: "repeat_bleach",
0x40000033: "enable_spot_bleach_pos",
0x40000034: "spot_bleach_posx",
0x40000035: "spot_bleach_posy",
0x40000036: "spot_bleach_posz",
0x40000037: "id_tubelens",
0x40000038: "id_tubelens_position",
0x40000039: "transmitted_light",
0x4000003a: "reflected_light",
0x4000003b: "simultan_grab_and_bleach",
0x4000003c: "bleach_pixel_time",
# detection_channels
0x70000001: "integration_mode",
0x70000002: "special_mode",
0x70000003: "detector_gain_first",
0x70000004: "detector_gain_last",
0x70000005: "amplifier_gain_first",
0x70000006: "amplifier_gain_last",
0x70000007: "amplifier_offs_first",
0x70000008: "amplifier_offs_last",
0x70000009: "pinhole_diameter",
0x7000000a: "counting_trigger",
0x7000000b: "acquire",
0x7000000c: "point_detector_name",
0x7000000d: "amplifier_name",
0x7000000e: "pinhole_name",
0x7000000f: "filter_set_name",
0x70000010: "filter_name",
0x70000013: "integrator_name",
0x70000014: "detection_channel_name",
0x70000015: "detection_detector_gain_bc1",
0x70000016: "detection_detector_gain_bc2",
0x70000017: "detection_amplifier_gain_bc1",
0x70000018: "detection_amplifier_gain_bc2",
0x70000019: "detection_amplifier_offset_bc1",
0x70000020: "detection_amplifier_offset_bc2",
0x70000021: "detection_spectral_scan_channels",
0x70000022: "detection_spi_wavelength_start",
0x70000023: "detection_spi_wavelength_stop",
0x70000026: "detection_dye_name",
0x70000027: "detection_dye_folder",
# illumination_channels
0x90000001: "name",
0x90000002: "power",
0x90000003: "wavelength",
0x90000004: "aquire",
0x90000005: "detchannel_name",
0x90000006: "power_bc1",
0x90000007: "power_bc2",
# beam_splitters
0xb0000001: "filter_set",
0xb0000002: "filter",
0xb0000003: "name",
# data_channels
0xd0000001: "name",
0xd0000003: "acquire",
0xd0000004: "color",
0xd0000005: "sample_type",
0xd0000006: "bits_per_sample",
0xd0000007: "ratio_type",
0xd0000008: "ratio_track1",
0xd0000009: "ratio_track2",
0xd000000a: "ratio_channel1",
0xd000000b: "ratio_channel2",
0xd000000c: "ratio_const1",
0xd000000d: "ratio_const2",
0xd000000e: "ratio_const3",
0xd000000f: "ratio_const4",
0xd0000010: "ratio_const5",
0xd0000011: "ratio_const6",
0xd0000012: "ratio_first_images1",
0xd0000013: "ratio_first_images2",
0xd0000014: "dye_name",
0xd0000015: "dye_folder",
0xd0000016: "spectrum",
0xd0000017: "acquire",
# markers
0x14000001: "name",
0x14000002: "description",
0x14000003: "trigger_in",
0x14000004: "trigger_out",
# timers
0x12000001: "name",
0x12000002: "description",
0x12000003: "interval",
0x12000004: "trigger_in",
0x12000005: "trigger_out",
0x12000006: "activation_time",
0x12000007: "activation_number",
}
# Map TIFF tag code to attribute name, default value, type, count, validator
TIFF_TAGS = {
254: ('new_subfile_type', 0, 4, 1, TIFF_SUBFILE_TYPES()),
255: ('subfile_type', None, 3, 1,
{0: 'undefined', 1: 'image', 2: 'reduced_image', 3: 'page'}),
256: ('image_width', None, 4, 1, None),
257: ('image_length', None, 4, 1, None),
258: ('bits_per_sample', 1, 3, 1, None),
259: ('compression', 1, 3, 1, TIFF_COMPESSIONS),
262: ('photometric', None, 3, 1, TIFF_PHOTOMETRICS),
266: ('fill_order', 1, 3, 1, {1: 'msb2lsb', 2: 'lsb2msb'}),
269: ('document_name', None, 2, None, None),
270: ('image_description', None, 2, None, None),
271: ('make', None, 2, None, None),
272: ('model', None, 2, None, None),
273: ('strip_offsets', None, 4, None, None),
274: ('orientation', 1, 3, 1, TIFF_ORIENTATIONS),
277: ('samples_per_pixel', 1, 3, 1, None),
278: ('rows_per_strip', 2**32-1, 4, 1, None),
279: ('strip_byte_counts', None, 4, None, None),
280: ('min_sample_value', None, 3, None, None),
281: ('max_sample_value', None, 3, None, None), # 2**bits_per_sample
282: ('x_resolution', None, 5, 1, None),
283: ('y_resolution', None, 5, 1, None),
284: ('planar_configuration', 1, 3, 1, {1: 'contig', 2: 'separate'}),
285: ('page_name', None, 2, None, None),
286: ('x_position', None, 5, 1, None),
287: ('y_position', None, 5, 1, None),
296: ('resolution_unit', 2, 4, 1, {1: 'none', 2: 'inch', 3: 'centimeter'}),
297: ('page_number', None, 3, 2, None),
305: ('software', None, 2, None, None),
306: ('datetime', None, 2, None, None),
315: ('artist', None, 2, None, None),
316: ('host_computer', None, 2, None, None),
317: ('predictor', 1, 3, 1, {1: None, 2: 'horizontal'}),
320: ('color_map', None, 3, None, None),
322: ('tile_width', None, 4, 1, None),
323: ('tile_length', None, 4, 1, None),
324: ('tile_offsets', None, 4, None, None),
325: ('tile_byte_counts', None, 4, None, None),
338: ('extra_samples', None, 3, None,
{0: 'unspecified', 1: 'assocalpha', 2: 'unassalpha'}),
339: ('sample_format', 1, 3, 1, TIFF_SAMPLE_FORMATS),
347: ('jpeg_tables', None, None, None, None),
530: ('ycbcr_subsampling', 1, 3, 2, None),
531: ('ycbcr_positioning', 1, 3, 1, None),
32997: ('image_depth', None, 4, 1, None),
32998: ('tile_depth', None, 4, 1, None),
33432: ('copyright', None, 1, None, None),
33445: ('md_file_tag', None, 4, 1, None),
33446: ('md_scale_pixel', None, 5, 1, None),
33447: ('md_color_table', None, 3, None, None),
33448: ('md_lab_name', None, 2, None, None),
33449: ('md_sample_info', None, 2, None, None),
33450: ('md_prep_date', None, 2, None, None),
33451: ('md_prep_time', None, 2, None, None),
33452: ('md_file_units', None, 2, None, None),
33550: ('model_pixel_scale', None, 12, 3, None),
33922: ('model_tie_point', None, 12, None, None),
37510: ('user_comment', None, None, None, None),
34665: ('exif_ifd', None, None, 1, None),
34735: ('geo_key_directory', None, 3, None, None),
34736: ('geo_double_params', None, 12, None, None),
34737: ('geo_ascii_params', None, 2, None, None),
34853: ('gps_ifd', None, None, 1, None),
42112: ('gdal_metadata', None, 2, None, None),
42113: ('gdal_nodata', None, 2, None, None),
50838: ('imagej_byte_counts', None, None, None, None),
50289: ('mc_xy_position', None, 12, 2, None),
50290: ('mc_z_position', None, 12, 1, None),
50291: ('mc_xy_calibration', None, 12, 3, None),
50292: ('mc_lens_lem_na_n', None, 12, 3, None),
50293: ('mc_channel_name', None, 1, None, None),
50294: ('mc_ex_wavelength', None, 12, 1, None),
50295: ('mc_time_stamp', None, 12, 1, None),
65200: ('flex_xml', None, 2, None, None),
# code: (attribute name, default value, type, count, validator)
}
# Map custom TIFF tag codes to attribute names and import functions
CUSTOM_TAGS = {
700: ('xmp', read_bytes),
34377: ('photoshop', read_numpy),
33723: ('iptc', read_bytes),
34675: ('icc_profile', read_numpy),
33628: ('mm_uic1', read_mm_uic1),
33629: ('mm_uic2', read_mm_uic2),
33630: ('mm_uic3', read_mm_uic3),
33631: ('mm_uic4', read_mm_uic4),
34361: ('mm_header', read_mm_header),
34362: ('mm_stamp', read_mm_stamp),
34386: ('mm_user_block', read_bytes),
34412: ('cz_lsm_info', read_cz_lsm_info),
43314: ('nih_image_header', read_nih_image_header),
# 40001: ('mc_ipwinscal', read_bytes),
40100: ('mc_id_old', read_bytes),
50288: ('mc_id', read_bytes),
50296: ('mc_frame_properties', read_bytes),
50839: ('imagej_metadata', read_bytes),
51123: ('micromanager_metadata', read_json),
}
# Max line length of printed output
PRINT_LINE_LEN = 79
def imshow(data, title=None, vmin=0, vmax=None, cmap=None,
bitspersample=None, photometric='rgb', interpolation='nearest',
dpi=96, figure=None, subplot=111, maxdim=8192, **kwargs):
"""Plot n-dimensional images using matplotlib.pyplot.
Return figure, subplot and plot axis.
Requires pyplot already imported ``from matplotlib import pyplot``.
Parameters
----------
bitspersample : int or None
Number of bits per channel in integer RGB images.
photometric : {'miniswhite', 'minisblack', 'rgb', or 'palette'}
The color space of the image data.
title : str
Window and subplot title.
figure : matplotlib.figure.Figure (optional).
Matplotlib to use for plotting.
subplot : int
A matplotlib.pyplot.subplot axis.
maxdim : int
maximum image size in any dimension.
kwargs : optional
Arguments for matplotlib.pyplot.imshow.
"""
#if photometric not in ('miniswhite', 'minisblack', 'rgb', 'palette'):
# raise ValueError("Can't handle %s photometrics" % photometric)
# TODO: handle photometric == 'separated' (CMYK)
isrgb = photometric in ('rgb', 'palette')
data = numpy.atleast_2d(data.squeeze())
data = data[(slice(0, maxdim), ) * len(data.shape)]
dims = data.ndim
if dims < 2:
raise ValueError("not an image")
elif dims == 2:
dims = 0
isrgb = False
else:
if isrgb and data.shape[-3] in (3, 4):
data = numpy.swapaxes(data, -3, -2)
data = numpy.swapaxes(data, -2, -1)
elif not isrgb and data.shape[-1] in (3, 4):
data = numpy.swapaxes(data, -3, -1)
data = numpy.swapaxes(data, -2, -1)
isrgb = isrgb and data.shape[-1] in (3, 4)
dims -= 3 if isrgb else 2
if photometric == 'palette' and isrgb:
datamax = data.max()
if datamax > 255:
data >>= 8 # possible precision loss
data = data.astype('B')
elif data.dtype.kind in 'ui':
if not (isrgb and data.dtype.itemsize <= 1) or bitspersample is None:
try:
bitspersample = int(math.ceil(math.log(data.max(), 2)))
except Exception:
bitspersample = data.dtype.itemsize * 8
elif not isinstance(bitspersample, int):
# bitspersample can be tuple, e.g. (5, 6, 5)
bitspersample = data.dtype.itemsize * 8
datamax = 2**bitspersample
if isrgb:
if bitspersample < 8:
data <<= 8 - bitspersample
elif bitspersample > 8:
data >>= bitspersample - 8 # precision loss
data = data.astype('B')
elif data.dtype.kind == 'f':
datamax = data.max()
if isrgb and datamax > 1.0:
if data.dtype.char == 'd':
data = data.astype('f')
data /= datamax
elif data.dtype.kind == 'b':
datamax = 1
elif data.dtype.kind == 'c':
raise NotImplementedError("complex type") # TODO: handle complex types
if not isrgb:
if vmax is None:
vmax = datamax
if vmin is None:
if data.dtype.kind == 'i':
dtmin = numpy.iinfo(data.dtype).min
vmin = numpy.min(data)
if vmin == dtmin:
vmin = numpy.min(data > dtmin)
if data.dtype.kind == 'f':
dtmin = numpy.finfo(data.dtype).min
vmin = numpy.min(data)
if vmin == dtmin:
vmin = numpy.min(data > dtmin)
else:
vmin = 0
pyplot = sys.modules['matplotlib.pyplot']
if figure is None:
pyplot.rc('font', family='sans-serif', weight='normal', size=8)
figure = pyplot.figure(dpi=dpi, figsize=(10.3, 6.3), frameon=True,
facecolor='1.0', edgecolor='w')
try:
figure.canvas.manager.window.title(title)
except Exception:
pass
pyplot.subplots_adjust(bottom=0.03*(dims+2), top=0.9,
left=0.1, right=0.95, hspace=0.05, wspace=0.0)
subplot = pyplot.subplot(subplot)
if title:
try:
title = unicode(title, 'Windows-1252')
except TypeError:
pass
pyplot.title(title, size=11)
if cmap is None:
if data.dtype.kind in 'ub' and vmin == 0:
cmap = 'gray'
else:
cmap = 'coolwarm'
if photometric == 'miniswhite':
cmap += '_r'
image = pyplot.imshow(data[(0, ) * dims].squeeze(), vmin=vmin, vmax=vmax,
cmap=cmap, interpolation=interpolation, **kwargs)
if not isrgb:
pyplot.colorbar() # panchor=(0.55, 0.5), fraction=0.05
def format_coord(x, y):
# callback function to format coordinate display in toolbar
x = int(x + 0.5)
y = int(y + 0.5)
try:
if dims:
return "%s @ %s [%4i, %4i]" % (cur_ax_dat[1][y, x],
current, x, y)
else:
return "%s @ [%4i, %4i]" % (data[y, x], x, y)
except IndexError:
return ""
pyplot.gca().format_coord = format_coord
if dims:
current = list((0, ) * dims)
cur_ax_dat = [0, data[tuple(current)].squeeze()]
sliders = [pyplot.Slider(
pyplot.axes([0.125, 0.03*(axis+1), 0.725, 0.025]),
'Dimension %i' % axis, 0, data.shape[axis]-1, 0, facecolor='0.5',
valfmt='%%.0f [%i]' % data.shape[axis]) for axis in range(dims)]
for slider in sliders:
slider.drawon = False
def set_image(current, sliders=sliders, data=data):
# change image and redraw canvas
cur_ax_dat[1] = data[tuple(current)].squeeze()
image.set_data(cur_ax_dat[1])
for ctrl, index in zip(sliders, current):
ctrl.eventson = False
ctrl.set_val(index)
ctrl.eventson = True
figure.canvas.draw()
def on_changed(index, axis, data=data, current=current):
# callback function for slider change event
index = int(round(index))
cur_ax_dat[0] = axis
if index == current[axis]:
return
if index >= data.shape[axis]:
index = 0
elif index < 0:
index = data.shape[axis] - 1
current[axis] = index
set_image(current)
def on_keypressed(event, data=data, current=current):
# callback function for key press event
key = event.key
axis = cur_ax_dat[0]
if str(key) in '0123456789':
on_changed(key, axis)
elif key == 'right':
on_changed(current[axis] + 1, axis)
elif key == 'left':
on_changed(current[axis] - 1, axis)
elif key == 'up':
cur_ax_dat[0] = 0 if axis == len(data.shape)-1 else axis + 1
elif key == 'down':
cur_ax_dat[0] = len(data.shape)-1 if axis == 0 else axis - 1
elif key == 'end':
on_changed(data.shape[axis] - 1, axis)
elif key == 'home':
on_changed(0, axis)
figure.canvas.mpl_connect('key_press_event', on_keypressed)
for axis, ctrl in enumerate(sliders):
ctrl.on_changed(lambda k, a=axis: on_changed(k, a))
return figure, subplot, image
def _app_show():
"""Block the GUI. For use as skimage plugin."""
pyplot = sys.modules['matplotlib.pyplot']
pyplot.show()
def main(argv=None):
"""Command line usage main function."""
if float(sys.version[0:3]) < 2.6:
print("This script requires Python version 2.6 or better.")
print("This is Python version %s" % sys.version)
return 0
if argv is None:
argv = sys.argv
import optparse
search_doc = lambda r, d: re.search(r, __doc__).group(1) if __doc__ else d
parser = optparse.OptionParser(
usage="usage: %prog [options] path",
description=search_doc("^([^\n]+)", ""),
version="%%prog %s" % search_doc(":Version: (.*)", "Unknown"))
opt = parser.add_option
opt('-p', '--page', dest='page', type='int', default=-1,
help="display single page")
opt('-s', '--series', dest='series', type='int', default=-1,
help="display series of pages of same shape")
opt('--nomultifile', dest='nomultifile', action='store_true',
default=False, help="don't read OME series from multiple files")
opt('--noplot', dest='noplot', action='store_true', default=False,
help="don't display images")
opt('--interpol', dest='interpol', metavar='INTERPOL', default='bilinear',
help="image interpolation method")
opt('--dpi', dest='dpi', type='int', default=96,
help="set plot resolution")
opt('--debug', dest='debug', action='store_true', default=False,
help="raise exception on failures")
opt('--test', dest='test', action='store_true', default=False,
help="try read all images in path")
opt('--doctest', dest='doctest', action='store_true', default=False,
help="runs the internal tests")
opt('-v', '--verbose', dest='verbose', action='store_true', default=True)
opt('-q', '--quiet', dest='verbose', action='store_false')
settings, path = parser.parse_args()
path = ' '.join(path)
if settings.doctest:
import doctest
doctest.testmod()
return 0
if not path:
parser.error("No file specified")
if settings.test:
test_tifffile(path, settings.verbose)
return 0
if any(i in path for i in '?*'):
path = glob.glob(path)
if not path:
print('no files match the pattern')
return 0
# TODO: handle image sequences
#if len(path) == 1:
path = path[0]
print("Reading file structure...", end=' ')
start = time.time()
try:
tif = TiffFile(path, multifile=not settings.nomultifile)
except Exception as e:
if settings.debug:
raise
else:
print("\n", e)
sys.exit(0)
print("%.3f ms" % ((time.time()-start) * 1e3))
if tif.is_ome:
settings.norgb = True
images = [(None, tif[0 if settings.page < 0 else settings.page])]
if not settings.noplot:
print("Reading image data... ", end=' ')
def notnone(x):
return next(i for i in x if i is not None)
start = time.time()
try:
if settings.page >= 0:
images = [(tif.asarray(key=settings.page),
tif[settings.page])]
elif settings.series >= 0:
images = [(tif.asarray(series=settings.series),
notnone(tif.series[settings.series].pages))]
else:
images = []
for i, s in enumerate(tif.series):
try:
images.append(
(tif.asarray(series=i), notnone(s.pages)))
except ValueError as e:
images.append((None, notnone(s.pages)))
if settings.debug:
raise
else:
print("\n* series %i failed: %s... " % (i, e),
end='')
print("%.3f ms" % ((time.time()-start) * 1e3))
except Exception as e:
if settings.debug:
raise
else:
print(e)
tif.close()
print("\nTIFF file:", tif)
print()
for i, s in enumerate(tif.series):
print ("Series %i" % i)
print(s)
print()
for i, page in images:
print(page)
print(page.tags)
if page.is_palette:
print("\nColor Map:", page.color_map.shape, page.color_map.dtype)
for attr in ('cz_lsm_info', 'cz_lsm_scan_information', 'mm_uic_tags',
'mm_header', 'imagej_tags', 'micromanager_metadata',
'nih_image_header'):
if hasattr(page, attr):
print("", attr.upper(), Record(getattr(page, attr)), sep="\n")
print()
if page.is_micromanager:
print('MICROMANAGER_FILE_METADATA')
print(Record(tif.micromanager_metadata))
if images and not settings.noplot:
try:
import matplotlib
matplotlib.use('TkAgg')
from matplotlib import pyplot
except ImportError as e:
warnings.warn("failed to import matplotlib.\n%s" % e)
else:
for img, page in images:
if img is None:
continue
vmin, vmax = None, None
if 'gdal_nodata' in page.tags:
vmin = numpy.min(img[img > float(page.gdal_nodata)])
if page.is_stk:
try:
vmin = page.mm_uic_tags['min_scale']
vmax = page.mm_uic_tags['max_scale']
except KeyError:
pass
else:
if vmax <= vmin:
vmin, vmax = None, None
title = "%s\n %s" % (str(tif), str(page))
imshow(img, title=title, vmin=vmin, vmax=vmax,
bitspersample=page.bits_per_sample,
photometric=page.photometric,
interpolation=settings.interpol,
dpi=settings.dpi)
pyplot.show()
TIFFfile = TiffFile # backwards compatibility
if sys.version_info[0] > 2:
basestring = str, bytes
unicode = str
if __name__ == "__main__":
sys.exit(main())
|