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-------------------------
NEW FEATURES
o Support GPos-based GRangesList objects.
o Add 'na.rm' argument to binnedAverage().
SIGNIFICANT USER-LEVEL CHANGES
o Change 'maxgap' and 'minoverlap' defaults for findOverlaps() and family
(i.e. countOverlaps(), overlapsAny(), and subsetByOverlaps()). This
change addresses 2 long-standing issues:
(1) by default zero-width ranges are not excluded anymore, and
(2) control of zero-width ranges and adjacent ranges is finally
decoupled (only partially though).
New default for 'minoverlap' is 0 instead of 1. New default for 'maxgap'
is -1 instead of 0. See ?findOverlaps for more information about 'maxgap'
and the meaning of -1. For example, if 'type' is "any", you need to set
'maxgap' to 0 if you want adjacent ranges to be considered as overlapping.
o GPos now extends GRanges but with a ranges slot that must be an IPos
object. Update "old" GPos objects with updateObject().
o Move pos() generic to IRanges package.
o Move rglist() generic to IRanges package.
o Rename GenomicRangesORmissing and GenomicRangesORGRangesList classes ->
GenomicRanges_OR_missing and GenomicRanges_OR_GRangesList, respectively.
o Remove "seqinfo" method for RangesList objects.
o Remove "stack" method for GenomicRangesList objects.
DEPRECATED AND DEFUNCT
o Remove 'force' argument from seqinfo() and seqlevels() setters (the
argument got deprecated in BioC 3.5 in favor of new and more flexible
'pruning.mode' argument).
BUG FIXES
o nearest() and distanceToNearest() now call findOverlaps() internally
with maxgap=0 and minoverlap=0. This fixes incorrect results obtained
in some situations e.g. in the situation reported here:
https://support.bioconductor.org/p/99369/ (zero-width ranges)
but also in this situation:
nearest(GRanges("chr1", IRanges(5, 10)),
GRanges("chr1", IRanges(1, 4:5)),
select="all")
where the 2 ranges in the subject are *both* nearest to the 5-10 range.
o '$' completion on GenomicRanges works in RStudio.
o Minor tweaks to conversion from character to GRanges and reverse
conversion.
CHANGES IN VERSION 1.28.0
-------------------------
NEW FEATURES
o Add coercion from ordinary list to GRangesList. Also the GRangesList()
constructor function now accepts a list of GRanges as input (and just
calls new coercion from list to GRangesList on it internally).
o seqlevels() setter now supports "fine" and "tidy" pruning modes on
GRangesList objects (in addition to "coarse" mode, which is the default).
o "range" methods now have a 'with.revmap' argument (like "reduce" and
"disjoin" methods).
o Add a bunch of range-oriented methods for GenomicRangesList objects.
SIGNIFICANT USER-LEVEL CHANGES
o Some changes/improvements to "precede" and "follow" methods for
GenomicRanges objects motivated by discussion on support site:
https://support.bioconductor.org/p/90664/
o Some changes/improvements to "rank" method for GenomicRanges objects:
- now supports the same ties methods as base::rank() (was only supporting
ties methods "first" and "min" until now)
- default ties method now is "average", like base::rank()
- now supports additional argument 'ignore.strand'.
DEPRECATED AND DEFUNCT
o Argument 'force' of seqinfo() and seqlevels() setters is deprecated in
favor of new and more flexible 'pruning.mode' argument.
BUG FIXES
o Fix severe performance regression introduced in Bioconductor 3.3 in
"intersect" and "setdiff" methods for GRangesList objects. Thanks to
Jens Reeder <reeder.jens@gene.com> for catching and reporting this.
CHANGES IN VERSION 1.26.0
-------------------------
NEW FEATURES
o Add 'with.revmap' argument to "reduce" method for GRangesList objects.
o Add 'with.revmap' argument to various "disjoin" methods.
o makeGRangesFromDataFrame() now tries to turn the "start" and "end"
columns of the input data frame into numeric vectors if they are not
already.
o Add makeGRangesListFromDataFrame() function.
o Add "summary" method for GenomicRanges objects.
o Add 'use.names' argument to the granges(), grglist(), and rglist()
generics and methods, as well as to a bunch of "ranges" methods (for
GRanges, GPos, GNCList, GRangesList, and DelegatingGenomicRanges).
Default is TRUE to preserve existing behavior.
o Add 'use.mcols' arguments to the "ranges" methods for GPos objects.
SIGNIFICANT USER-LEVEL CHANGES
DEPRECATED AND DEFUNCT
BUG FIXES
o Fix bug in distanceToNearest() related to ranges starting at zero.
o Fix GRanges(Seqinfo()).
CHANGES IN VERSION 1.24.0
-------------------------
NEW FEATURES
o Add the GPos class, a container for storing a set of "genomic positions"
(i.e. genomic ranges of width 1). Even though a GRanges object can be
used for that, using a GPos object can be much more memory-efficient,
especially when the object contains long runs of adjacent positions.
o Add a bunch of "invertStrand" methods to support strand inversion of any
"stranded" object (i.e. any object with a strand() getter and setter).
E.g. invertStrand() works on GRanges, GRangesList, GAlignments,
GAlignmentPairs, GAlignmentsList, and RangedSummarizedExperiment objects.
o Add "is.unsorted" method for GenomicRanges objects (contributed by Pete
Hickey).
o base::rank() gained a new 'ties.method="last"' option and base::order()
a new argument ('method') in R 3.3. Thus so do the "rank" and "order"
methods for GenomicRanges objects.
o Add "selfmatch" method for GenomicRanges objects.
o Add "union" method for GRangesList objects.
SIGNIFICANT USER-LEVEL CHANGES
o Remove old SummarizedExperiment class from the GenomicRanges package
(this class is now defined in the SummarizedExperiment package).
o Move the following generic functions from the GenomicRanges package to
the SummarizedExperiment package:
- SummarizedExperiment
- exptData, "exptData<-"
- rowRanges, "rowRanges<-"
- colData, "colData<-"
- assayNames, "assayNames<-"
- assays, "assays<-"
- assay, "assay<-"
o Rename "pintersect" and "psetdiff" methods for GRangesList objects ->
"intersect" and "setdiff" without changing their behavior (they still
do mendoapply(intersect, x, y) and mendoapply(setdiff, x, y),
respectively). The old names were misnomers (see svn commit message for
commit 113793 for more information).
o Remove the ellipsis (...) from all the setops methods, except from:
- "punion" method for signature GRanges#GRangesList;
- "pintersect" and "psetdiff" methods for signature
GRangesList#GRangesList;
- "pgap" method for GRanges objects.
o Use DESeq2 instead of DESeq in the vignettes (better late than never).
DEPRECATED AND DEFUNCT
o Remove GIntervalTree class and methods (were defunct in BioC 3.2).
o Remove mapCoords() and pmapCoords() (were defunct in BioC 3.2).
CHANGES IN VERSION 1.22.0
-------------------------
NEW FEATURES
o Support coercions back and forth between a GRanges object and a character
vector (or factor) with elements in the format 'chr1:2501-2800' or
'chr1:2501-2800:+'.
o Add facilities for manipulating "genomic variables": bindAsGRanges(),
mcolAsRleList(), and binnedAverage(). See ?genomicvars for more
information.
o Add "narrow" method for GRangesList objects.
o Enhancement to the GRanges() constructor. If the 'ranges' argument is not
supplied then the constructor proceeds in 2 steps:
1. An initial GRanges object is created with 'as(seqnames, "GRanges")'.
2. Then this GRanges object is updated according to whatever other
arguments were supplied to the call to GRanges().
Because of this enhancement, GRanges(x) is now equivalent to
'as(x, "GRanges")' e.g. GRanges() can be called directly on a character
vector representing ranges, or on a data.frame, or on any object for
which coercion to GRanges is supported.
o Add 'ignore.strand' argument to "range" and "reduce" methods for
GRangesList objects.
o Add coercion from SummarizedExperiment to RangedSummarizedExperiment
(also available via updateObject()). See 1st item in DEPRECATED AND
DEFUNCT section below for more information about this.
o GNCList objects are now subsettable.
o "coverage" methods now accept 'shift' and 'weight' supplied as an Rle.
SIGNIFICANT USER-LEVEL CHANGES
o Modify behavior of "*" strand in precede() / follow() to mimic
'ignore.strand=TRUE'.
o Revisit "pintersect" methods for GRanges#GRanges, GRangesList#GRanges,
and GRanges#GRangesList:
- Sanitize their semantic.
- Add 'drop.nohit.ranges' argument (FALSE by default).
- If 'drop.nohit.ranges' is FALSE, the returned object now has a "hit"
metadata column added to it to indicate the elements in 'x' that
intersect with the corresponding element in 'y'.
o binnedAverage() now treats 'numvar' as if it was set to zero on genomic
positions where it's not set (typically happens when 'numvar' doesn't
span the entire chromosomes because it's missing the trailing zeros).
o GRanges() constructor no more mangles the names of the supplied metadata
columns (e.g. if the column is "_tx_id").
o makeGRangesFromDataFrame() now accepts "." in strand column (treated as
"*").
o GNCList() constructor now propagates the metadata columns.
o Remove "seqnames" method for RangesList objects.
DEPRECATED AND DEFUNCT
o The SummarizedExperiment class defined in GenomicRanges is deprecated
and replaced by 2 new classes defined in the new SummarizedExperiment
package: SummarizedExperiment0 and RangedSummarizedExperiment.
In BioC 2.3, the SummarizedExperiment class will be removed from the
GenomicRanges package and the SummarizedExperiment0 class will be renamed
SummarizedExperiment. To facilitate this transition, a coercion method
was added to coerce from old SummarizedExperiment to new
RangedSummarizedExperiment (this coercion is performed when calling
updateObject() on an old SummarizedExperiment object).
o makeSummarizedExperimentFromExpressionSet() and related stuff was moved
to the new SummarizedExperiment package.
o After being deprecated in BioC 3.1, the rowData accessor is now defunct
(replaced with the rowRanges accessor).
o After being deprecated in BioC 3.1, GIntervalTree objects and the
"intervaltree" algorithm in findOverlaps() are now defunct.
o After being deprecated in BioC 3.1, mapCoords() and pmapCoords() are
now defunct.
BUG FIXES
o 2 tweaks to subsetting *by* an GenomicRanges:
- Improve speed when the object to subset is a SimpleList (e.g.
SimpleRleList).
- Fix issue when the GenomicRanges subscript is empty.
CHANGES IN VERSION 1.20.0
-------------------------
NEW FEATURES
o Add coercion methods to go back and forth between ExpressionSet and
SummarizedExperiment.
o Add 'assayNames', 'assayNames<-' for SummarizedExperiment
o assays() supports arrays of up to 4 dimensions.
o Add GNCList() for preprocessing a GenomicRanges object into a GNCList
object that can be used for fast overlap seach with findOverlaps().
GNCList() is a replacement for GIntervalTree() that uses Nested
Containment Lists instead of interval trees. Unlike GIntervalTree(),
GNCList() supports preprocessing of a GenomicRanges object with
ranges located on a circular sequence. For a one time use, it's not
advised to explicitely preprocess the input. This is because
findOverlaps() or countOverlaps() will take care of it and do a better
job at it (that is, they preprocess only what's needed when it's needed
and release memory as they go).
o All "findOverlaps" methods now support 'select' equal "last" or
"arbitrary" (in addition to "all" and "first").
o Add absoluteRanges() and relativeRanges() to transform back and forth
between absolute and relative genomic ranges.
SIGNIFICANT USER-LEVEL CHANGES
o Renamed 'rowData' and 'rowData<-' -> 'rowRanges', 'rowRanges<-'.
Old names still work but are deprecated.
o Some improvements to makeGRangesFromDataFrame():
- Improve internal logic used for finding the GRanges columns in the
input.
- If 'seqinfo' is not supplied, the seqlevels are now ordered according
to the output of GenomeInfoDb::rankSeqlevels().
- Now an attempt is made to turn 'df' into a data frame (with
'as.data.frame(df)') if it's not a data frame or a DataFrame object.
o The GRanges() constructor now propagates the metadata cols that are on
'ranges' if no metadata cols are explicitly passed to the constructor.
DEPRECATED AND DEFUNCT
o Deprecated 'rowData' and 'rowData<-' in favor of 'rowRanges' and
'rowRanges<-'.
o Deprecated mapCoords() and pmapCoords(). They're replaced by
mapToTranscripts() and pmapToTranscripts() from the GenomicFeatures
package and mapToAlignments() and pmapToAlignments() from the
GenomicAlignments package.
o Deprecated GIntervalTree objects.
o Removed "map" and "splitAsListReturnedClass" methods (were defunct in
GenomicRanges 1.18.0).
o Removed makeSeqnameIds() (was defunct in GenomicRanges 1.18.0).
o Removed 'with.mapping' argunment from "reduce" methods (was defunct in
GenomicRanges 1.18.0).
BUG FIXES
o Fix 'findOverlaps(..., type="start")' on GRangesList objects which has
been broken for years.
o Fix self overlap search on a GRanges object when 'ignore.strand=TRUE'
(i.e. 'findOverlaps(gr, ignore.strand=TRUE)').
CHANGES IN VERSION 1.18.0
-------------------------
NEW FEATURES
o Add 'use.mcols' arg to "ranges" method for GRangesList objects.
o "assays<-" methods may be invoked with 'withDimnames' arg.
o Add mapCoords() generic and methods (replacing map()).
o Add granges,GenomicRanges method.
o Add strand<-,GRangesList,character method for global replacement
(i.e., all strands become 'value').
o Add resize,GRangesList-method.
o Add DelegatingGenomicRanges class and vignette on how to extend
GenomicRanges.
o Document subsetting a named list-like object by a GRanges subscript.
SIGNIFICANT USER-LEVEL CHANGES
o Modify "show" methods for GRanges and GRangesList objects so
they print a 1-line summary of the seqinfo component.
o Remove as.data.frame,GRangesList-method; use as.data.frame,List.
o "trim" method for GenomicRanges only trims out-of-bound
ranges on non-circular sequences whose length is not NA.
This behavior is consistent with the GenomicRanges validity method.
o Changes to flank(), resize() and start/end/width setters:
- no longer trim the result ranges when called on a GRanges
- warning is issued by GenomicRanges validity method when
out-of-bound ranges are on non-circular sequences whose
length is not NA
Note this behavior is now consistent with that of shift().
o Speed up validation of GenomicRanges objects by 1.2x.
o Speed up trim() on GenomicRanges objects by 1.2x.
o Improve warning when GenomicRanges object contains out-of-bound ranges.
o Work on vignette HOWTOs:
- split 'How to read BAM files into R' into 3 HOWTOs
- split 'How to prepare a table of read counts for RNA-Seq
differential gene expression' into 3 HOWTOs
- split 'How to extract DNA sequences of gene regions' into 2 HOWTOs
- make individual HOWTOs subsections of single HOWTO section
o Follow renaming of TranscriptDb class to TxDb.
o Replace references to plantsmart21 with plantsmart22.
DEPRECATED AND DEFUNCT
o Defunct map() (skip deprecation). Replace with mapCoords().
BUG FIXES
o [cr]bind,SummarizedExperiment methods respect derived classes.
o assays(se, withDimnames=TRUE) <- value no longer tries to access
a slot 'withDimnames'.
o cbind and rbind,SummarizedExperiment-methods respect derived
classes
o "ranges" method for GRangesList objects should not propagate
inner metadata columns by default.
o GRanges() constructor now preserves the seqlevels in the order
supplied by the user.
o Ensure tileGenome() breakpoints do not extend past end of genome.
o Fix "show" method for GenomicRanges objects when 'showHeadLines'
global option is set to Inf.
o [rc]bind,SummarizeExperiment-methods now compare all elements.
o Remove "==" and "<=" methods for GenomicRanges objects (not needed).
CHANGES IN VERSION 1.16.0
-------------------------
NEW FEATURES
o Add "subset" method for SummarizedExperiment objects.
o Allow DataFrame in SummarizedExperiment assays.
o Add 'use.mcols' arg (FALSE by default) to the granges(), grglist(), and
rglist() generics (a.k.a. the range-squeezer generics).
o Add coercion method from GRangesList to RangesList.
o Add score() setter for GRangesList objects.
o findOverlaps(..., type="within") now works on circular chromosomes.
o Add 'ignore.strand' arg to "sort" method for GRanges objects.
o Support subsetting of a named list-like object *by* a GenomicRanges
subscript.
o Support sort(granges, by = ~ score), i.e., a formula-based interface for
sorting by the mcols.
SIGNIFICANT USER-LEVEL CHANGES
o Move many functionalities to the new GenomicAlignments package:
- The GAlignments, GAlignmentPairs, and GAlignmentsList classes.
- The qnarrow() generic and methods.
- The "narrow" and "pintersect" methods for GAlignments and
GAlignmentsList objects.
- The low-level CIGAR utilities.
- The "findOverlaps" methods for GAlignment* objects.
- The summarizeOverlaps() generic and methods, and the "Counting reads
with summarizeOverlaps" vignette.
- findCompatibleOverlaps() and countCompatibleOverlaps().
- The findSpliceOverlaps() generic and methods.
- The "overlap encodings" stuff i.e. the "encodeOverlaps" method for
GRangesList objects, flipQuery(), selectEncodingWithCompatibleStrand(),
isCompatibleWithSplicing(), isCompatibleWithSkippedExons(),
extractSteppedExonRanks(), extractSpannedExonRanks(),
extractSkippedExonRanks(), and extractQueryStartInTranscript(), and
the "OverlapEncodings" vignette.
o Rename 'with.mapping' arg -> 'with.revmap' in "reduce" methods. The old
arg name is still working but deprecated.
o Move makeSeqnameIds() function to the new GenomeInfoDb package and rename
it rankSeqlevels(). The old name is still working but deprecated.
o The "strand" methods now perform stricter checking and are guaranteed
to always return a factor (or factor-Rle) with the "standard strand
levels" and no NAs. Or to fail.
BUG FIXES
o Tweaks and fixes to various "strand" methods:
- Methods for character vectors and factors do not accept NAs anymore
(they raise an error).
- Methods for integer and logical vectors map NAs to * (instead of NA).
- Method for Rle objects now also works on character-, factor-, and
integer-Rle objects (in addition to logical-Rle objects).
CHANGES IN VERSION 1.14.0
-------------------------
NEW FEATURES
o Add coercion from GenomicRangesList to RangedDataList.
o Add "c" method for GAlignmentPairs objects.
o Add coercion from GAlignmentPairs to GAlignmentsList.
o Add 'inter.feature' and 'fragment' arguments to summarizeOverlaps().
o Add seqselect,GAlignments-method.
o Add CIGAR utilities:
explodeCigarOps(), explodeCigarOpLengths()
cigarRangesAlongReferenceSpace(), cigarRangesAlongQuerySpace()
cigarRangesAlongPairwiseSpace(), extractAlignmentRangesOnReference()
cigarWidthAlongReferenceSpace(), cigarWidthAlongQuerySpace()
cigarWidthAlongPairwiseSpace().
o Add seqlevels0() and restoreSeqlevels().
o Add seqlevelsInUse() getter for GRanges, GRangesList, GAlignments
GAlignmentPairs, GAlignmentsList and SummarizedExperiment objects.
o Add update,GAlignments method.
o Add GIntervalTree class.
o Add coercion from GAlignmentPairs to GAlignments.
o Add sortSeqlevels().
o Add tileGenome().
o Add makeGRangesFromDataFrame() and coercion from data.frame or DataFrame
to GRanges.
SIGNIFICANT USER-LEVEL CHANGES
o Renaming (with aliases from old to new names):
- classes
GappedAlignments -> GAlignments
GappedAlignmentPairs -> GAlignmentPairs
- functions
GappedAlignments() -> GAlignments()
GappedAlignmentPairs() -> GAlignmentPairs()
readGappedAlignments() -> readGAlignments()
readGappedAlignmentPairs() -> readGAlignmentPairs()
o Remove 'asProperPairs' argument to readGAlignmentsList().
o Modify "show" method for Seqinfo object to honor showHeadLines
and showTailLines global options.
o 50x speedup or more when merging 2 Seqinfo objects, 1 very small
and 1 very big.
o Add dependency on new XVector package.
o Enhanced examples for renaming seqlevels in seqlevels-utils.Rd.
o More efficient reference class constructor for 'assays'
slot of SummarizedExperiment objects.
o 'colData' slot of SummarizedExperiment produced from call to
summarizedOverlaps() now holds the class type and length of 'reads'.
o 4x speedup to cigarToRleList().
o Relax SummarizedExperiment class validity.
o Renaming (with aliases from old to new names):
cigarToWidth() -> cigarWidthOnReferenceSpace(), and
cigarToQWidth() -> cigarWidthOnQuerySpace().
o Improvements to summarizeOverlaps():
- mode 'Union': 1.5x to 2x speedup
- mode 'IntersectionNotEmpty': 2x to 8x speedup + memory footprint
reduced by ~ half
o Change default 'use.names' to FALSE for readGAlignmentsList().
o Implement 'type="equal"' for findOverlaps,SummarizedExperiment methods.
o Modify summarizeOverlaps() examples to use 'asMates=TRUE' instead
of 'obeyQname=TRUE'.
o Remove unneeded "window" method for GenomicRanges objects.
o Speed up seqinfo() getter and setter on SummarizedExperiment objects
and derivatives (e.g. VCF) by using direct access to 'rowData' slot.
o coverage,GenomicRanges method now uses .Ranges.coverage() when
using the defaults for 'shift' and 'width'.
o Remove restriction that metadata column names must be different on
a GRangesList and the unlisted GRanges.
o GenomicRangesUseCases vignette has been redone and renamed
to GenomicRangesHOWTOs
DEPRECATED AND DEFUNCT
o Defunct all "match" and "%in%" methods in the
package except for those with the GenomicRanges,GenomicRanges signature.
o Deprecate GappedAlignment*:
- GappedAlignments and GappedAlignmentPairs classes
- GappedAlignments() and GappedAlignmentPairs() constructors
- readGappedAlignments() and readGappedAlignmentPairs() functions
o Deprecate cigar util functions:
cigarToWidth(), cigarToQWidth(), cigarToIRanges()
splitCigar(), cigarToIRanges(), cigarToIRangesListByAlignment()
cigarToIRangesListByRName(), cigarToWidth(), cigarToQWidth()
cigarToCigarTable(), summarizeCigarTable()
o Deprecate seqselect().
BUG FIXES
o Fix bug in c,GAlignments for case when objects were unnamed.
o Fix bug in flank,GenomicRanges (when 'ignore.strand=TRUE'
'start' was being set to TRUE).
o Fix bug in behavior of summarizeOverlaps() count mode
'IntersectionNotEmpty' when 'inter.features=FALSE'. Shared
regions are now removed before counting.
o Fix bug in cigarToIRangesListByAlignment() when 'flag' is
supplied and indicates some reads are unmapped.
o Fix bug in summarizeOverlaps(..., mode='IntersectionNotEmpty')
when 'features' has '-' and '+' elements and 'ignore.strand=TRUE'.
o match,GenomicRanges,GenomicRanges method now handles properly objects
with seqlevels not in the same order.
CHANGES IN VERSION 1.12.0
-------------------------
NEW FEATURES
o Implement "seqnameStyle" replacement method for Seqinfo object.
'seqnameStyle(x) <- style' works on any object with a "seqinfo"
replacement method.
o Add trim,GenomicRanges-method to trim out of bound ranges.
o Add promoters,GenomicRanges and promoters,GRangesList methods.
o Add "overlapsAny" methods as a replacement for the deprecated
"%in%" methods.
o Add 'ignore.strand' argument to match,GenomicRanges-method.
o Add 'with.mapping' argument to "reduce" method for GenomicRanges
objects.
o Add "unname" method to remove dimnames from SummarizedExperiment.
o Add "cbind" and "rbind" methods for SummarizedExperiment.
o Add "seqselect", "seqselect<-" and "split" methods for
SummarizedExperiment.
o Add GAlignmentsList class.
o Add readGAlignmentsList generic and methods.
SIGNIFICANT USER-LEVEL CHANGES
o resize,GenomicRanges method no longer checks that 'fix' is
length-compatible with 'x' when 'x' is length zero. This
allows for resize(x, w, fix = "end") without worrying about
'x' being zero-length.
o Change the behavior of "distance". Previously adjacent ranges
had a distance of 1 and overlapping had a distance of 0. Now
both adjacent AND overlapping have a distance of 0.
o shift,GenomicRanges-method no longer trims out of bound ranges.
o "distanceToNearest" no longer drops ranges that have no hit
but returns 'NA' for 'subjectHits' and 'distance'.
o "genome" is no longer an invalid metadata colname for GenomicRanges
objects.
o 4x-8x speedup for doing coverage() on a GRanges or GRangesList with
many seqlevels.
o Remove ">=", "<", and ">" methods for GenomicRanges objects.
o Speedup "seqinfo" setters for GenomicRanges and GappedAlignments
by avoiding validation when not necessary.
o readGappedAlignments can now pass a BamFile to readBamGappedAlignments.
o Remove unneeded "unique" and "sort" methods for GenomicRanges objects.
o Change behavior of "match" and "%in%" on GenomicRanges objects to use
equality instead of overlap for comparing elements between GenomicRanges
objects 'x' and 'table'.
o match,GenomicRanges-method gets the same 'method' argumnet as the
"duplicated" method for these objects.
o Remove unneeded "countOverlaps" methods.
o "classNameForDisplay" shortens the name of data type when displayed.
o Add global options 'showHeadLines' and 'showTailLines' to
control the number of head/tails lines displayed in
show,GRanges and show,GappedAlignments methods.
o "distanceToNearest" now returns a Hits object instead of DataFrame.
DEPRECATED AND DEFUNCT
o Remove defunct countGenomicOverlaps(), grg(), and globalToQuery()
o Defunct previously deprecated '.ignoreElementMetadata' argmuent
of c,GenomicRanges-method.
o Deprecate all "match" and "%in%" methods in the package except for
those with the GenomicRanges,GenomicRanges signature.
o Deprecate "resolveHits" methods.
BUG FIXES
o Several bug fixes to "nearest".
o Output of "findSpliceOverlaps" now displays 'NA' for ranges with
no hits.
CHANGES IN VERSION 1.10.0
-------------------------
NEW FEATURES
o SummarizedExperiment gains direct GRanges / GRangesList
interface to rowData.
o Add "distanceToNearest" method for GenomicRanges objects.
o SummarizedExperiment class can now be subset by row when there are no
'columns', and by column when there are no 'rows'.
o Add 'drop.D.ranges' argument to coverage,GappedAlignments and
coverage,GappedAlignmentPairs methods.
o findOverlaps() now supports 'select="last"' and 'select="arbitrary"' (in
addition to 'select="all"' and 'select="first"') on GenomicRanges
objects.
o summarizeOverlaps(..., mode="IntersectionStrict") now handles circular
chromosomes. A warning is issued and circular chromosomes in 'reads' are
omitted from counting.
o Add disjoin,GRangesList method.
o Add findSpliceOverlaps() for identifyng ranges (reads) that are
compatible with a specific transcript isoform (the non-compatible
ranges are analyzed for the presence of novel splice events).
o Add ngap,GappedAlignmentPairs method.
o Add introns() generic with methods for GappedAlignments and
GappedAlignmentPairs objects.
o No more arbitrary max of 3 gaps per read in isCompatibleWithSplicing()
and isCompatibleWithSkippedExons().
o Add findCompatibleOverlaps() and countCompatibleOverlaps().
o Passing '...' down through as.data.frame(GRanges, ...) so user can tweak
stringsAsFactors default for metadata columns.
o Add extractSteppedExonRanks(), extractSpannedExonRanks() and
extractQueryStartInTranscript() utilities (work with single- and
paired-end reads).
o Add 'flip.query.if.wrong.strand' arg (FALSE by default) to
"encodeOverlaps" method for GRangesList objects.
o Add makeSeqnameIds() low-level utility.
SIGNIFICANT USER-LEVEL CHANGES
o SummarizedExperiment rowData and assays operations have
significant performance improvements.
o mcols() is now the preferred way (over elementMetadata() or values()) to
access the metadata columns of a GenomicRanges, GRangesList,
GappedAlignments, GappedAlignmentPairs, SummarizedExperiment object,
or any Vector object. elementMetadata() and values() might go away at
some point in the (not so close) future.
o Add "$" and "$<-" methods for GenomicRanges *only*. Provided as a
convenience and as the result of strong popular demand. Note that those
methods are not consistent with the other "$" and "$<-" methods in the
IRanges/GenomicRanges infrastructure, and might confuse some users by
making them believe that a GenomicRanges object can be manipulated as a
data.frame-like object. It is therefore recommended to use them only
interactively, and their use in scripts or packages is discouraged.
For the latter, use 'mcols(x)$name' instead of 'x$name'.
o No more warning when doing as(x, "GRanges") on a RangedData object with
no "strand" column.
o Refactor "[" method for GenomicRanges objects. The new implementation
always preserves the names of the selected elements instead of trying
to return a GenomicRanges object with unique names. This new behavior
is consistent with subsetting of ordinary vectors and other Vector
objects defined in IRanges/GenomicRanges. Also modify "seqselect" method
for GenomicRanges objects so it also preserves the names of the selected
elements (and thus remains consistent with new behavior of "[" method for
GenomicRanges objects).
o No more names on the integer vector returned by "ngap" method for
GappedAlignments objects.
o Many improvements to the "Overlap encodings" vignette.
o Remove 'param' argument from summarizeOverlaps() generic.
DEPRECATED AND DEFUNCT
o Defunct previously deprecated grg() function.
o Defunct previously deprecated countGenomicOverlaps() generic and methods.
BUG FIXES
o Fix several issues with "precede", "follow", "nearest", and "distance"
methods for GenomicRanges objects.
o Fix bug in summarizeOverlaps(..., ignore.strand=TRUE).
o 6x speedup (and a 6x memory footprint reduction) or more when using
encodeOverlaps() on big GRangesList objects.
o Fix bug in renameSeqlevels() wrt order of rename vector.
o Fix bug in selectEncodingWithCompatibleStrand().
CHANGES IN VERSION 1.8.0
------------------------
NEW FEATURES
o Add GappedAlignmentPairs class (with accessors first(), last(), left(),
right(), seqnames(), strand(), isProperPair()), and
readGappedAlignmentPairs() for dealing with paired-end reads.
Most of the GappedAlignments functionalities (e.g. coercion to
GRangesList, "findOverlaps" and related methods, "coverage", etc...)
work on a GappedAlignmentPairs object.
o Add encodeOverlaps,GRangesList,GRangesList,missing and related utilities
flipQuery(), selectEncodingWithCompatibleStrand(),
isCompatibleWithSplicing(), isCompatibleWithSkippedExons() and
extractSkippedExonRanks().
o Add 'order.as.in.query' arg to grglist() and rglist().
o SummarizedExperiment gains direct access to colData columns with
$, $<-, [[, and [[<- methods
o Add map,GenomicRanges,GRangesList and
map,GenomicRanges,GappedAlignments methods. These allow mapping
from genome space to transcript space, and genome space to read
space, respectively.
o Add seqinfo methods (and friends) for RangedData, RangesList,
and other IRanges data structures. These use metadata(x)$seqinfo.
o Add disjointBins,GenomicRanges.
o Add score,GRangesList and score,GenomicRanges (gets the score column
like for RangedData).
o Add RangedDataList -> GenomicRangesList coercion.
o Add RleViewsList -> GRanges coercion.
o Add pintersect,GRangesList,GRangesList
o Add stack,GenomicRangesList
o ignore.strand argument now more uniformly supported on set operations.
o Add Ops,GenomicRanges (from rtracklayer).
o Add strand,Rle (only logical-Rle is supported).
o Add compare,GenomicRanges
o Add 'drop.empty.ranges' arg (FALSE by default) to low-level cigar
utilities cigarToIRanges(), cigarToIRangesListByAlignment(), and
cigarToIRangesListByRName().
o Add 'reduce.ranges' arg to cigarToIRangesListByAlignment().
SIGNIFICANT USER-LEVEL CHANGES
o grglist,GappedAlignments now carries over metadata columns.
o Names are no longer forced to be unique when unlisting a
GRangesList with use.names=TRUE.
o seqnames() is now preferred over rname() on a GappedAlignments object.
o cigarToIRangesListByAlignment() now returns a CompressedIRangesList
instead of CompressedNormalIRangesList.
o Low-level CIGAR utilities now ignore CIGAR operation P (instead of
trowing an error).
o The 'weight' arg in "coverage" method for GenomicRanges objects now
can also be a single string naming a column in elementMetadata(x).
o Ranges outside the sequences bounds of the underlying sequences are now
accepted (with a warning) in GenomicRanges/GRangesList/GappedAlignments
objects.
o When called with 'ignore.strand=TRUE', the "range" and "disjoin" methods
for GenomicRanges objects now behave like if they set the strand of the
input to "*" before they do any computation.
o When called with 'ignore.strand=TRUE', "reduce" method for GenomicRanges
objects, and "union", "intersect" and "setdiff" methods for GRanges
objects now set the strand of their arguments to "*" prior to any
computation.
o No more mangling of the names when combining GRanges objects ("c" method
for GRanges objects was trying to return unique names).
o Remove isCircularWithKnownLength() generic and methods (nobody knows,
uses, or needs this).
BUG FIXES
o flank,GRangesList no longer forces 'use.names' to TRUE and 'both' to
FALSE.
o range,GenomicRanges was broken when object had no ranges
o Fix integer overflow issue that can occur in cigarQNarrow() or
cigarQNarrow() when the cigar vector is very long.
CHANGES IN VERSION 1.6.0
------------------------
NEW FEATURES
o seqlevels() and seqinfo() setters have a new arg ('force', default is
FALSE) to force dropping sequence levels currently in use.
o Seqinfo objects now have a genome column that can be accessed with
genome() getter/setter.
o "pgap" method for c(x="GRanges", y="GRanges").
o Add comparison (==, <=, duplicated, unique, etc...) and ordering
(order, sort, rank) methods for GenomicRanges objects.
o Add "flank" method for GRangesList objects.
o Add "isDisjoint" and "restrict" methods for GRanges and GRangesList
objects.
o Add GRangesList constructor makeGRangesListFromFeatureFragments().
o Add "names" and "names<-" methods for GappedAlignments objects.
o Add 'ignore.strand' arg to a number of methods:
- findOverlaps,GRangesList,RangesList
- findOverlaps,GappedAlignments,ANY
- findOverlaps,ANY,GappedAlignments
o 'shift' and 'weight' arguments of "coverage" method for GenomicRanges
objects now can be numeric vectors in addition to lists.
o Add "c" method for GappedAlignments objects.
SIGNIFICANT USER-VISIBLE CHANGES
o readGappedAlignments() supports 2 new arguments: (1) 'use.names' (default
is FALSE) for using the query template names (QNAME field in a SAM/BAM
file) to set the names of the returned object, and (2) 'param' (default
is NULL, otherwise a ScanBamParam object) for controlling what fields and
which records are imported. readGappedAlignments() doesn't support
the 'which' arg anymore.
o The names of a GRanges/GRangesList/GappedAlignments object are not
required to be unique anymore.
o By default, the rownames are not set anymore on the DataFrame returned
by elementMetadata() on a GRanges/GRangesList/GappedAlignments object.
o 'width' arg of "coverage" method for GenomicRanges objects now must be
NULL or numeric vector (instead of NULL or list).
DEPRECATED AND DEFUNCT
o Deprecate countGenomicOverlaps() in favor of summarizeOverlaps().
o Deprecate grg() in favor of granges().
BUG FIXES
o Fix bug in "pintersect" methods operating on GappedAlignments objects.
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