gff2aplot 2.0-9 / usr / bin / blat2gff

#!/usr/bin/perl -w
#
# ##################################################################
# #                          blat2gff.pl                           #
# ##################################################################
# 
#         blat2gff.pl [options] < inputfile > outputfile
#
#       Converts BLAT output files to GFF formatted files.
# 
#     Copyright (C) 2001-2003 -- Josep Francesc ABRIL FERRANDO  
#
# This program is free software; you can redistribute it and/or modify
# it under the terms of the GNU General Public License as published by
# the Free Software Foundation; either version 2 of the License, or
# (at your option) any later version.
# 
# This program is distributed in the hope that it will be useful,
# but WITHOUT ANY WARRANTY; without even the implied warranty of
# MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE.  See the
# GNU General Public License for more details.
# 
# You should have received a copy of the GNU General Public License
# along with this program; if not, write to the Free Software
# Foundation, Inc., 675 Mass Ave, Cambridge, MA 02139, USA.
# 
# ##################################################################
#
# $Id: blat2gff.nw,v 1.2 2003/02/28 17:21:12 jabril Exp $
#
use strict;

#
my $PROGRAM = 'blat2gff.pl';
my $VERSION = '1.0';
my $DATE = localtime;
my $USER = defined( $ENV{USER} ) ? $ENV{USER} : 'Child Process';
my $host = `hostname`;
chomp($host);

#
#
# MODULES
#

#
# VARIABLES
#
my @frame = ( 3, 1, 2 );
my %blat = (
    STRAND => 8,
    Q_NAME => 9,
    Q_LEN  => 10,
    Q_ORI  => 11,
    Q_END  => 12,
    T_NAME => 13,
    T_LEN  => 14,
    T_ORI  => 15,
    T_END  => 16,
    B_NUM  => 17,
    B_LEN  => 18,
    B_QPOS => 19,
    B_TPOS => 20,
);
my %gff       = ();
my $maxfields = 21;
my %group     = ();
my ( $group_cnt, $rec_cnt ) = (0) x 2;

# GetSRsAln.pl like:
#$srs->{QUERY}\t$blst_prg\tsr\t$srs->{START_Q}\t$srs->{END_Q}
#\t$srs->{SCORE}\t$srs->{STRAND_Q}\t$srs->{FRAME_Q}
#\tTarget \"$srs->{SUBJECT}\"\t$srs->{START_S}\t$srs->{END_S}
#\tE_value $srs->{E_VALUE}\tStrand $srs->{STRAND_S}
#\tFrame $srs->{FRAME_S}\t\#Projection $srs->{PROJECTION} 
my $GFFstring =
  ( "\%s\t" x 8 ) . 'Target "%s"' . "\t\%s\t\%s;\tStrand \%s;\tFrame \%s\n";

#
# MAIN LOOP
#
while (<STDIN>) {
    my @f = ();
    next unless /^\d/o;
    chomp;
    @f = split /\s+/o, $_;
    do {    # ensure that there are 21 fields
        print STDERR "### NOT enough fields for this record:\n##### @f \n";
        next;
    } unless scalar(@f) == $maxfields;
    %gff = ();
    ++$rec_cnt;
    $f[ $blat{STRAND} ] =~ /(.)(.)/og
      && ( $gff{Q_STRAND} = $1, $gff{T_STRAND} = $2 );
    do {    # ensure that strands are +/-
        print STDERR "### CANNOT find strand for this record:\n##### @f \n";
        next;
    } unless ( $gff{Q_STRAND} =~ /[+-]/o && $gff{T_STRAND} =~ /[+-]/o );
    $f[ $blat{Q_NAME} ] =~ m{/?([^/]+)$}og && ( $gff{Q_NAME} = $1 );
    $f[ $blat{T_NAME} ] =~ m{/?([^/]+)$}og && ( $gff{T_NAME} = $1 );
    defined( $group{"$gff{T_NAME}.$gff{T_STRAND}"} )
      || ( $group{"$gff{T_NAME}.$gff{T_STRAND}"} = ++$group_cnt );
    $gff{GROUP} = $group{"$gff{T_NAME}.$gff{T_STRAND}"};
    $gff{Q_LEN} = $f[ $blat{Q_LEN} ];                      # seq lengths are OK
    $gff{T_LEN} = $f[ $blat{T_LEN} ];
    $gff{Q_ORI}   = $f[ $blat{Q_ORI} ] + 1;    # HSP coords start at 0, not at 1
    $gff{Q_END}   = $f[ $blat{Q_END} ] + 1;
    $gff{T_ORI}   = $f[ $blat{T_ORI} ] + 1;
    $gff{T_END}   = $f[ $blat{T_END} ] + 1;
    $gff{Q_FRAME} =
      &get_frame( $gff{Q_STRAND}, $gff{Q_LEN}, $gff{Q_ORI}, $gff{Q_END} );
    $gff{T_FRAME} =
      &get_frame( $gff{T_STRAND}, $gff{T_LEN}, $gff{T_ORI}, $gff{T_END} );
    $gff{B_NUM} = $f[ $blat{B_NUM} ];
    @{ $gff{B_LEN} }  = split /,/o, $f[ $blat{B_LEN} ];
    @{ $gff{B_QPOS} } = split /,/o, $f[ $blat{B_QPOS} ];
    @{ $gff{B_TPOS} } = split /,/o, $f[ $blat{B_TPOS} ];
    printf STDOUT "# " . $GFFstring, $gff{Q_NAME}, "BLAT",
      "$gff{Q_LEN}:$gff{T_LEN}", $gff{Q_ORI}, $gff{Q_END}, 0, $gff{Q_STRAND},
      $gff{Q_FRAME}, "$gff{T_NAME}.$gff{GROUP}.$rec_cnt", $gff{T_ORI},
      $gff{T_END}, $gff{T_STRAND}, $gff{T_FRAME};
    &loop_HSPs($rec_cnt);
};    # while read input

exit(0);

#
# FUNCTIONS
#
sub get_frame() {
    my ( $strand, $len, $ori, $end ) = @_;
    if ( $strand eq '-' ) {
        return $frame[ ( ( $len - $end + 1 ) % 3 ) ];
    }
    else {
        return $frame[ ( $ori % 3 ) ];
    }
}    # get_frame

sub get_hsp() {
    my ( $s, $L, $l, $n ) = @_;
    my ( $o, $e );
    if ( $s eq '-' ) {
        $e = $L - $n;
        $o = $e - $l;
    }
    else {
        $o = $n;
        $e = $o + $l;
    }
    $o++;
    $e++;    # HSP coords start at 0, not at 1
    return ( $o, $e, &get_frame( $s, $L, $o, $e ) );
}    # get_hsp

sub loop_HSPs() {
    my ($num) = @_;
    my ( $c, $l, $q, $t, $qo, $qe, $qf, $to, $te, $tf );
    for ( $c = 0 ; $c < $gff{B_NUM} ; $c++ ) {
        $l = $gff{B_LEN}[$c];
        $q = $gff{B_QPOS}[$c];
        $t = $gff{B_TPOS}[$c];
        ( $qo, $qe, $qf ) = &get_hsp( $gff{Q_STRAND}, $gff{Q_LEN}, $l, $q );
        ( $to, $te, $tf ) = &get_hsp( $gff{T_STRAND}, $gff{T_LEN}, $l, $t );
        printf STDOUT $GFFstring, $gff{Q_NAME}, "blat", "hsp", $qo, $qe, 0,
          $gff{Q_STRAND}, $qf, "$gff{T_NAME}.$gff{GROUP}.$num", $to, $te,
          $gff{T_STRAND}, $tf;
    };    # for
}    # loop_HSPs