/usr/bin/mipe2pcrprimers is in mipe 1.1-6.
This file is owned by root:root, with mode 0o755.
The actual contents of the file can be viewed below.
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 | #!/usr/bin/perl
# This library is free software; you can redistribute it and/or
# modify it under the terms of the GNU Lesser General Public
# License as published by the Free Software Foundation; either
# version 2.1 of the License, or (at your option) any later version.
#
# This library is distributed in the hope that it will be useful,
# but WITHOUT ANY WARRANTY; without even the implied warranty of
# MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the GNU
# Lesser General Public License for more details.
#
# You should have received a copy of the GNU Lesser General Public
# License along with this library ('COPYING'); if not, write to the Free Software
# Foundation, Inc., 51 Franklin St, Fifth Floor, Boston, MA 02110-1301 USA
use strict;
use warnings;
use XML::Twig;
=head1 NAME
mipe2pcrprimers.pl - Generates list of pcrprimers from a MIPE file
based on MIPE version v1.1
arguments: * mipe_file
* (optional) list of PCR IDs
=head1 SYNOPSIS
mipe2pcrprimers.pl your_file.mipe <pcr_id1> <pcr_id2>
=head1 ADDITIONAL INFO
See http://mipe.sourceforge.net
=head1 AUTHOR
Jan Aerts (jan.aerts@bbsrc.ac.uk)
=cut
my ( $file, @pcr_ids ) = @ARGV;
if ( not defined $file ) { die "Please provide filename\n" };
my $twig = XML::Twig->new( TwigHandlers => { pcr => \&pcr }
, pretty_print => 'indented' );
$twig->parsefile($file);
exit;
sub pcr {
my ( $twig, $pcr ) = @_;
my $to_include = 0;
my $pcr_id = $pcr->{att}->{id};
if ( scalar @pcr_ids > 0 ) {
$to_include = 0;
foreach ( @pcr_ids ) {
if ( $pcr_id =~ /$_/i ) {
$to_include = 1;
}
}
} else {
$to_include = 1;
}
if ( $to_include ) {
my $primer1 = $pcr->next_elt('design')->next_elt('primer1');
my $primer1_oligo = ( defined $primer1->first_child('oligo') ) ? $primer1->first_child('oligo')->text : 'NO OLIGO';
my $primer1_seq = ( defined $primer1->first_child('seq') ) ? $primer1->first_child('seq')->text : 'NO SEQ';
my $primer1_tm = ( defined $primer1->first_child('tm') ) ? $primer1->first_child('tm')->text : 'NO TM';
my $primer2 = $pcr->next_elt('design')->next_elt('primer2');
my $primer2_oligo = ( defined $primer2->first_child('oligo') ) ? $primer2->first_child('oligo')->text : 'NO OLIGO';
my $primer2_seq = ( defined $primer2->first_child('seq') ) ? $primer2->first_child('seq')->text : 'NO SEQ';
my $primer2_tm = ( defined $primer2->first_child('tm') ) ? $primer2->first_child('tm')->text : 'NO TM';
print $pcr_id, "\tFW\t", $primer1_oligo, "\t", $primer1_seq, "\t", $primer1_tm, "\tREV\t", $primer2_oligo, "\t", $primer2_seq, "\t", $primer2_tm, "\n";
}
}
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