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DEFINITION E.coli lactose operon with lacI, lacZ, lacY and lacA genes.
ACCESSION J01636 J01637 K01483 K01793
VERSION J01636.1 GI:146575
KEYWORDS acetyltransferase; beta-D-galactosidase; galactosidase; lac operon;
lac repressor protein; lacA gene; lacI gene; lacY gene; lacZ gene;
lactose permease; mutagenesis; palindrome; promoter region;
thiogalactoside acetyltransferase.
SOURCE Escherichia coli
ORGANISM Escherichia coli
Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
Enterobacteriaceae; Escherichia.
REFERENCE 1 (bases 1243 to 1266)
AUTHORS Gilbert,W. and Maxam,A.
TITLE The nucleotide sequence of the lac operator
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 70 (12), 3581-3584 (1973)
PUBMED 4587255
REFERENCE 2 (bases 1246 to 1308)
AUTHORS Maizels,N.M.
TITLE The nucleotide sequence of the lactose messenger ribonucleic acid
transcribed from the UV5 promoter mutant of Escherichia coli
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 70 (12), 3585-3589 (1973)
PUBMED 4587256
REFERENCE 3 (sites)
AUTHORS Gilbert,W., Maizels,N. and Maxam,A.
TITLE Sequences of controlling regions of the lactose operon
JOURNAL Cold Spring Harb. Symp. Quant. Biol. 38, 845-855 (1974)
PUBMED 4598642
REFERENCE 4 (sites)
AUTHORS Gilbert,W., Gralla,J., Majors,A.J. and Maxam,A.
TITLE Lactose operator sequences and the action of lac repressor
JOURNAL (in) Sund,H. and Blauer,G. (Eds.);
PROTEIN-LIGAND INTERACTIONS: 193-207;
Walter de Gruyter, New York (1975)
REFERENCE 5 (bases 1146 to 1282)
AUTHORS Dickson,R.C., Abelson,J., Barnes,W.M. and Reznikoff,W.S.
TITLE Genetic regulation: the Lac control region
JOURNAL Science 187 (4171), 27-35 (1975)
PUBMED 1088926
REFERENCE 6 (bases 1227 to 1271)
AUTHORS Gilbert,W., Maxam,A. and Mirzabekov,A.
TITLE Contacts between the lac repressor and DNA revealed by methylation
JOURNAL (in) Kjeldgaard,N.C. and Maaloe,O. (Eds.);
CONTROL OF RIBOSOME SYNTHESIS: 138-143;
Academic Press, New York (1976)
REFERENCE 7 (sites)
AUTHORS Marians,K.J. and Brooker,J.D.
TITLE Structure of the lactose operator
JOURNAL Nature 260 (5549), 360-363 (1976)
PUBMED 768781
REFERENCE 8 (bases 1242 to 1268)
AUTHORS Heyneker,H.L., Shine,J., Goodman,H.M., Boyer,H.W., Rosenberg,J.,
Dickerson,R.E., Narang,S.A., Itakura,K., Lin,S. and Riggs,A.D.
TITLE Synthetic lac operator DNA is functional in vivo
JOURNAL Nature 263 (5580), 748-752 (1976)
PUBMED 1069185
REFERENCE 9 (sites)
AUTHORS Dickson,R.C., Abelson,J. and Johnson,P.
TITLE Nucleotide sequence changes produced by mutations in the lac
promoter of Escherichia coli
JOURNAL J. Mol. Biol. 111 (1), 65-75 (1977)
PUBMED 323498
REFERENCE 10 (bases 51 to 264)
AUTHORS Steege,D.A.
TITLE 5'-Terminal nucleotide sequence of Escherichia coli lactose
repressor mRNA: features of translational initiation and
reinitiation sites
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 74 (10), 4163-4167 (1977)
PUBMED 337294
REFERENCE 11 (bases 1 to 81)
AUTHORS Calos,M.P.
TITLE DNA sequence for a low-level promoter of the lac repressor gene and
an 'up' promoter mutation
JOURNAL Nature 274 (5673), 762-765 (1978)
PUBMED 355890
REFERENCE 12 (bases 49 to 1161)
AUTHORS Farabaugh,P.J.
TITLE Sequence of the lacI gene
JOURNAL Nature 274 (5673), 765-769 (1978)
PUBMED 355891
REFERENCE 13 (sites)
AUTHORS Miller,J.H., Coulondre,C. and Farabaugh,P.J.
TITLE Correlation of nonsense sites in the lacI gene with specific codons
in the nucleotide sequence
JOURNAL Nature 274 (5673), 770-775 (1978)
PUBMED 355892
REFERENCE 14 (sites)
AUTHORS Calos,M.P. and Miller,J.H.
TITLE DNA sequence alteration resulting from a mutation impairing
promoter function in the lac repressor gene
JOURNAL Mol. Gen. Genet. 178 (1), 225-227 (1980)
PUBMED 6770231
REFERENCE 15 (bases 4306 to 5804)
AUTHORS Buchel,D.E., Gronenborn,B. and Muller-Hill,B.
TITLE Sequence of the lactose permease gene
JOURNAL Nature 283 (5747), 541-545 (1980)
PUBMED 6444453
REFERENCE 16 (sites)
AUTHORS Miller,J.H., Calos,M.P. and Galas,D.J.
TITLE Genetic and sequencing studies of the specificity of transposition
into the lac region of E. coli
JOURNAL Cold Spring Harb. Symp. Quant. Biol. 45 PT 1, 243-257 (1981)
PUBMED 6271472
REFERENCE 17 (sites)
AUTHORS Chenchick,A., Beabealashvilli,R. and Mirzabekov,A.
TITLE Topography of interaction of Escherichia coli RNA polymerase
subunits with lac UV5 promoter
JOURNAL FEBS Lett. 128 (1), 46-50 (1981)
PUBMED 7023981
REFERENCE 18 (sites)
AUTHORS Betz,J.L. and Sadler,J.R.
TITLE Variants of a cloned synthetic lactose operator. I. A palindromic
dimer lactose operator derived from one stand of the cloned 40-base
pair operator
JOURNAL Gene 13 (1), 1-12 (1981)
PUBMED 7016667
REFERENCE 19 (sites)
AUTHORS Sadler,J.R. and Tecklenburg,M.
TITLE Cloning and characterization of the natural lactose operator
JOURNAL Gene 13 (1), 13-23 (1981)
PUBMED 6263752
REFERENCE 20 (sites)
AUTHORS Betz,J.L. and Sadler,J.R.
TITLE Variants of a cloned synthetic lactose operator. II.
Chloramphenicol-resistant revertants retaining a lactose operator
in the CAT gene of plasmid pBR325
JOURNAL Gene 15 (2-3), 187-200 (1981)
PUBMED 6271642
REFERENCE 21 (sites)
AUTHORS Calos,M.P. and Miller,J.H.
TITLE The DNA sequence change resulting from the IQ1 mutation, which
greatly increases promoter strength
JOURNAL Mol. Gen. Genet. 183 (3), 559-560 (1981)
PUBMED 7038381
REFERENCE 22 (sites)
AUTHORS Mieschendahl,M., Buchel,D., Bocklage,H. and Muller-Hill,B.
TITLE Mutations in the lacY gene of Escherichia coli define functional
organization of lactose permease
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 78 (12), 7652-7656 (1981)
PUBMED 6278484
REFERENCE 23 (sites)
AUTHORS Russell,D.R. and Bennett,G.N.
TITLE Construction and analysis of in vivo activity of E. coli promoter
hybrids and promoter mutants that alter the -35 to -10 spacing
JOURNAL Gene 20 (2), 231-243 (1982)
PUBMED 6299890
REFERENCE 24 (sites)
AUTHORS Horowitz,H. and Platt,T.
TITLE A termination site for LacI transcription is between the CAP site
and the lac promoter
JOURNAL J. Biol. Chem. 257 (19), 11740-11746 (1982)
PUBMED 6288696
REFERENCE 25 (sites)
AUTHORS Klein,R.D. and Wells,R.D.
TITLE Effects of neighboring DNA homopolymers on the biochemical and
physical properties of the Escherichia coli lactose promoter. I.
Cloning and characterization studies
JOURNAL J. Biol. Chem. 257 (21), 12954-12961 (1982)
PUBMED 6290487
REFERENCE 26 (bases 1183 to 1291)
AUTHORS Weiher,H. and Schaller,H.
TITLE Segment-specific mutagenesis: extensive mutagenesis of a lac
promoter/operator element
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 79 (5), 1408-1412 (1982)
PUBMED 7041119
REFERENCE 27 (sites)
AUTHORS Van Dyke,M.W. and Dervan,P.B.
TITLE Footprinting with MPE.Fe(II). Complementary-strand analyses of
distamycin- and actinomycin-binding sites on heterogeneous DNA
JOURNAL Cold Spring Harb. Symp. Quant. Biol. 47 PT 1, 347-353 (1983)
PUBMED 6305557
REFERENCE 28 (bases 1287 to 4364)
AUTHORS Kalnins,A., Otto,K., Ruther,U. and Muller-Hill,B.
TITLE Sequence of the lacZ gene of Escherichia coli
JOURNAL EMBO J. 2 (4), 593-597 (1983)
PUBMED 6313347
REFERENCE 29 (sites)
AUTHORS Cone,K.C., Sellitti,M.A. and Steege,D.A.
TITLE Lac repressor mRNA transcription terminates in vivo in the lac
control region
JOURNAL J. Biol. Chem. 258 (18), 11296-11304 (1983)
PUBMED 6309841
REFERENCE 30 (sites)
AUTHORS Sadler,J.R., Sasmor,H. and Betz,J.L.
TITLE A perfectly symmetric lac operator binds the lac repressor very
tightly
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 80 (22), 6785-6789 (1983)
PUBMED 6316325
REFERENCE 31 (sites)
AUTHORS Glickman,B.W. and Ripley,L.S.
TITLE Structural intermediates of deletion mutagenesis: a role for
palindromic DNA
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 81 (2), 512-516 (1984)
PUBMED 6582506
REFERENCE 32 (sites)
AUTHORS Spassky,A., Kirkegaard,K. and Buc,H.
TITLE Changes in the DNA structure of the lac UV5 promoter during
formation of an open complex with Escherichia coli RNA polymerase
JOURNAL Biochemistry 24 (11), 2723-2731 (1985)
PUBMED 3896305
REFERENCE 33 (sites)
AUTHORS Straney,D.C. and Crothers,D.M.
TITLE Intermediates in transcription initiation from the E. coli lac UV5
promoter
JOURNAL Cell 43 (2 PT 1), 449-459 (1985)
PUBMED 2416465
REFERENCE 34 (sites)
AUTHORS Looman,A.C., de Gruyter,M., Vogelaar,A. and van Knippenberg,P.H.
TITLE Effects of heterologous ribosomal binding sites on the
transcription and translation of the lacZ gene of Escherichia coli
JOURNAL Gene 37 (1-3), 145-154 (1985)
PUBMED 3932130
REFERENCE 35 (sites)
AUTHORS Mandecki,W., Goldman,R.A., Powell,B.S. and Caruthers,M.H.
TITLE lac Up-promoter mutants with increased homology to the consensus
promoter sequence
JOURNAL J. Bacteriol. 164 (3), 1353-1355 (1985)
PUBMED 2999082
REFERENCE 36 (sites)
AUTHORS Malamy,M.H., Rahaim,P.T., Hoffman,C.S., Baghdoyan,D., O'Connor,M.B.
and Miller,J.F.
TITLE A frameshift mutation at the junction of an IS1 insertion within
lacZ restores beta-galactosidase activity via formation of an
active lacZ-IS1 fusion protein
JOURNAL J. Mol. Biol. 181 (4), 551-555 (1985)
PUBMED 2987506
REFERENCE 37 (bases 5646 to 7477)
AUTHORS Hediger,M.A., Johnson,D.F., Nierlich,D.P. and Zabin,I.
TITLE DNA sequence of the lactose operon: the lacA gene and the
transcriptional termination region
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 82 (19), 6414-6418 (1985)
PUBMED 3901000
COMMENT Original source text: Escherichia coli DNA; mRNA; clone
lambda-h80dlac DNA; clone puk217; pgm8 (see comment).
[3] sites; UV5 mRNA transcripts and operator mutants. [(in)
Sund,H. and Blauer,G. (eds.);Protein-Ligand Interactions:
193-207;Walter de] sites; operator mutational analysis. [7]
sites; S1 and mung bean nuclease action on operator DNA. [9]
sites; class I, II and III promoter mutant analysis. [13] sites;
lacI mutant analysis.
[16] sites; Tn5, Tn9 and Tn10 insertion sites in lac region. [14]
sites; lacI promoter mutation UJ177.
[18] sites; palindromic dimer operator;.
[19] sites; natural operator sequence.
[20] sites; operator mutational analysis.
[21] sites; lacI-Q deletion.
[17] sites; RNA polymerase UV5 promoter interaction. [22] sites;
lacY mutational analysis.
[24] sites; lacI-promoted transcription termination. [25] sites;
wt and UV5 promoter sequence studies. [23] sites; UV5 promoter
mutational analysis.
[30] sites; perfectly symmetric operator sequence. [29] sites;
lacI mRNA termination site.
[27] sites; distamycin and actinomycin binding to promoter. [31]
sites; lacI deletion studies.
[35] sites; promoter mutational studies.
[33] sites; DNAase I studies with promoter sequence. [34] sites;
ribosomal binding and translation initiation for lacZ. [36] sites;
insertion sequence IS1 integration in lacZ;. [32] sites; DNAase I
studies with promoter.
[1] first reports a 27 bp operator(sites 1240-1266) with two-fold
symmetries; the operator has also been defined to be bases
1246-1266 or bases 1239-1273 [8]. [(in) Kjeldgaard,N.C. and Maaloe,
O.(eds);Control of ribosome synthesis: 138-143;A] explores the
ability of lac
repressor protein to affect methylation of operator DNA. [8]
argues that DNA on both sides of the 21 bp operator (bases
1246-1266) affects repressor binding but that the sequences of this
DNA are probably not critical. [5] gives a larger sequence known as
the promoter-operator region for the wild-type, whereas [2] and
[26] give portions of this region for the mutant strain UV5. Within
the promoter region, bases 1162-1199 are identified as the
catabolite gene activator protein binding site (cap) and bases
1200-1245 are the RNA polymerase interaction site. [10] reports a
sequence for the 5'end of the lacI (repressor) gene and discusses
restart in mutant strains. [11] presents a sequence for the lacI
promoter region and identifies an I-Q mutation which enhances lacI
transcription approximately ten-fold. [12] gives a complete
sequence
for lacI which agrees with the known lac repressor sequence. [26]
examines the promoter-operator region in the UV5 strain (lac109)
and studies 23 mutant derivatives of this sequence. This sequence
agrees with known protein sequences for the lacZ, lacY and lacA
enzymes. [15] notes that the fMet codon is not present
for lacA and suggests that the 'ttg' codon (5727-5729), which
immediately precedes the mature N-terminal asparagine codon, is the
start codon. The cds for lacZ, lacY and lacA are included on a
single mRNA transcript.
Complete source information:
Escherichia coli DNA [1],[(in) Kjeldgaard,N.C. and Maaloe,O.(eds);
Control of ribosome synthesis: 138-143;A],[8],[12],[26]; mRNA [2],
[5],[10]; clone
lambda-h80dlac DNA [11],[15]; clone puk217 [28]; pgm8 [37].
FEATURES Location/Qualifiers
source 1. .7477
/organism="Escherichia coli"
/mol_type="genomic DNA"
/db_xref="taxon:562"
variation 16
/note="c in wild-type; t in 'up' promoter mutant I-Q [11]"
mRNA 51. .1230
/note="lacI (repressor) mRNA; preferred in vivo 3' end
[12],[29]"
gene 79. .1161
/gene="lacI"
CDS 79. .1161
/gene="lacI"
/note="lac repressor protein (gtg start codon)"
/codon_start=1
/transl_table=11
/protein_id="AAA24052.1"
/db_xref="GI:146576"
/translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAE
LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS
GVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSII
FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREG
DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDT
EDSSCYIPPSTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNT
QTASPRALADSLMQLARQVSRLESGQ"
misc_signal 1162. .1199
/note="cap protein binding site"
variation 1183. .1186
/note="ttag in wild-type; aatt in strain UV5 [26]"
variation 1209. .1211
/note="gct in wild-type; gt in mutant l305 [5]"
variation 1212
/note="t in wild-type; a in mutant l241 [5]"
variation 1230
/note="c in wild-type; a in mutant p-r-1a [5]"
variation 1237. .1238
/note="gt in wild-type; aa in strain UV5 [26]"
variation 1242. .1245
/note="gtgg in wild-type; ttca in synthetic operator [8]"
mRNA 1246. .>4358
/note="lacZ mRNA [2],[5]"
misc_signal 1246. .1266
/note="lac repressor protein binding site"
variation 1267. .1268
/note="tc in wild-type; tg in synthetic operator [8]"
variation 1282. .1291
/note="ctatgaccat in wild-type; gatccggcca in strain UV5
[26]"
gene 1284. .4358
/gene="lacZ"
CDS 1284. .4358
/gene="lacZ"
/note="beta-d-galactosidase"
/codon_start=1
/transl_table=11
/protein_id="AAA24053.1"
/db_xref="GI:146577"
/translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEAR
TDRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYT
NVTYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWV
GYGQDSRLPSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHK
PTTQISDFHVATRFNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFG
GEIIDERGGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFR
EVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSH
YPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRN
HPSVIIWSLGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVD
EDQPFPAVPKWSIKKWLSLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGG
FVWDWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQ
QQFFQFRLSGQTIEVTSEYLFRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIE
LPELPQPESAGQLWLTVRVVQPNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPH
LTTSEMDFCIELGNKRWQFNRQSGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSE
ATRIDPNAWVERWKAAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKT
YRIDGSGQMAITVDVEVASDTPHPARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAA
CFDRWDLPLSDMYTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETS
HRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQK"
gene 4410. .5663
/gene="lacY"
CDS 4410. .5663
/gene="lacY"
/note="lactose permease"
/codon_start=1
/transl_table=11
/protein_id="AAA24054.1"
/db_xref="GI:146578"
/translation="MYYLKNTNFWMFGLFFFFYFFIMGAYFPFFPIWLHDINHISKSD
TGIIFAAISLFSLLFQPLFGLLSDKLGLRKYLLWIITGMLVMFAPFFIFIFGPLLQYN
ILVGSIVGGIYLGFCFNAGAPAVEAFIEKVSRRSNFEFGRARMFGCVGWALCASIVGI
MFTINNQFVFWLGSGCALILAVLLFFAKTDAPSSATVANAVGANHSAFSLKLALELFR
QPKLWFLSLYVIGVSCTYDVFDQQFANFFTSFFATGEQGTRVFGYVTTMGELLNASIM
FFAPLIINRIGGKNALLLAGTIMSVRIIGSSFATSALEVVILKTLHMFEVPFLLVGCF
KYITSQFEVRFSATIYLVCFCFFKQLAMIFMSVLAGNMYESIGFQGAYLVLGLVALGF
TLISVFTLSGPGPLSLLRRQVNEVA"
gene 5727. .6338
/gene="lacA"
CDS 5727. .6338
/gene="lacA"
/note="thiogalactoside acetyltransferase (ttg start
codon)"
/codon_start=1
/transl_table=11
/protein_id="AAA24055.1"
/db_xref="GI:551814"
/translation="MNMPMTERIRAGKLFTDMCEGLPEKRLRGKTLMYEFNHSHPSEV
EKRESLIKEMFATVGENAWVEPPVYFSYGSNIHIGRNFYANFNLTIVDDYTVTIGDNV
LIAPNVTLSVTGHPVHHELRKNGEMYSFPITIGNNVWIGSHVVINPGVTIGDNSVIGA
GSIVTKDIPPNVVAAGVPCRVIREINDRDKHYYFKDYKVESSV"
ORIGIN
1 GACACCATCG AATGGCGCAA AACCTTTCGC GGTATGGCAT GATAGCGCCC GGAAGAGAGT
61 CAATTCAGGG TGGTGAATGT GAAACCAGTA ACGTTATACG ATGTCGCAGA GTATGCCGGT
121 GTCTCTTATC AGACCGTTTC CCGCGTGGTG AACCAGGCCA GCCACGTTTC TGCGAAAACG
181 CGGGAAAAAG TGGAAGCGGC GATGGCGGAG CTGAATTACA TTCCCAACCG CGTGGCACAA
241 CAACTGGCGG GCAAACAGTC GTTGCTGATT GGCGTTGCCA CCTCCAGTCT GGCCCTGCAC
301 GCGCCGTCGC AAATTGTCGC GGCGATTAAA TCTCGCGCCG ATCAACTGGG TGCCAGCGTG
361 GTGGTGTCGA TGGTAGAACG AAGCGGCGTC GAAGCCTGTA AAGCGGCGGT GCACAATCTT
421 CTCGCGCAAC GCGTCAGTGG GCTGATCATT AACTATCCGC TGGATGACCA GGATGCCATT
481 GCTGTGGAAG CTGCCTGCAC TAATGTTCCG GCGTTATTTC TTGATGTCTC TGACCAGACA
541 CCCATCAACA GTATTATTTT CTCCCATGAA GACGGTACGC GACTGGGCGT GGAGCATCTG
601 GTCGCATTGG GTCACCAGCA AATCGCGCTG TTAGCGGGCC CATTAAGTTC TGTCTCGGCG
661 CGTCTGCGTC TGGCTGGCTG GCATAAATAT CTCACTCGCA ATCAAATTCA GCCGATAGCG
721 GAACGGGAAG GCGACTGGAG TGCCATGTCC GGTTTTCAAC AAACCATGCA AATGCTGAAT
781 GAGGGCATCG TTCCCACTGC GATGCTGGTT GCCAACGATC AGATGGCGCT GGGCGCAATG
841 CGCGCCATTA CCGAGTCCGG GCTGCGCGTT GGTGCGGATA TCTCGGTAGT GGGATACGAC
901 GATACCGAAG ACAGCTCATG TTATATCCCG CCGTCAACCA CCATCAAACA GGATTTTCGC
961 CTGCTGGGGC AAACCAGCGT GGACCGCTTG CTGCAACTCT CTCAGGGCCA GGCGGTGAAG
1021 GGCAATCAGC TGTTGCCCGT CTCACTGGTG AAAAGAAAAA CCACCCTGGC GCCCAATACG
1081 CAAACCGCCT CTCCCCGCGC GTTGGCCGAT TCATTAATGC AGCTGGCACG ACAGGTTTCC
1141 CGACTGGAAA GCGGGCAGTG AGCGCAACGC AATTAATGTG AGTTAGCTCA CTCATTAGGC
1201 ACCCCAGGCT TTACACTTTA TGCTTCCGGC TCGTATGTTG TGTGGAATTG TGAGCGGATA
1261 ACAATTTCAC ACAGGAAACA GCTATGACCA TGATTACGGA TTCACTGGCC GTCGTTTTAC
1321 AACGTCGTGA CTGGGAAAAC CCTGGCGTTA CCCAACTTAA TCGCCTTGCA GCACATCCCC
1381 CTTTCGCCAG CTGGCGTAAT AGCGAAGAGG CCCGCACCGA TCGCCCTTCC CAACAGTTGC
1441 GCAGCCTGAA TGGCGAATGG CGCTTTGCCT GGTTTCCGGC ACCAGAAGCG GTGCCGGAAA
1501 GCTGGCTGGA GTGCGATCTT CCTGAGGCCG ATACTGTCGT CGTCCCCTCA AACTGGCAGA
1561 TGCACGGTTA CGATGCGCCC ATCTACACCA ACGTAACCTA TCCCATTACG GTCAATCCGC
1621 CGTTTGTTCC CACGGAGAAT CCGACGGGTT GTTACTCGCT CACATTTAAT GTTGATGAAA
1681 GCTGGCTACA GGAAGGCCAG ACGCGAATTA TTTTTGATGG CGTTAACTCG GCGTTTCATC
1741 TGTGGTGCAA CGGGCGCTGG GTCGGTTACG GCCAGGACAG TCGTTTGCCG TCTGAATTTG
1801 ACCTGAGCGC ATTTTTACGC GCCGGAGAAA ACCGCCTCGC GGTGATGGTG CTGCGTTGGA
1861 GTGACGGCAG TTATCTGGAA GATCAGGATA TGTGGCGGAT GAGCGGCATT TTCCGTGACG
1921 TCTCGTTGCT GCATAAACCG ACTACACAAA TCAGCGATTT CCATGTTGCC ACTCGCTTTA
1981 ATGATGATTT CAGCCGCGCT GTACTGGAGG CTGAAGTTCA GATGTGCGGC GAGTTGCGTG
2041 ACTACCTACG GGTAACAGTT TCTTTATGGC AGGGTGAAAC GCAGGTCGCC AGCGGCACCG
2101 CGCCTTTCGG CGGTGAAATT ATCGATGAGC GTGGTGGTTA TGCCGATCGC GTCACACTAC
2161 GTCTGAACGT CGAAAACCCG AAACTGTGGA GCGCCGAAAT CCCGAATCTC TATCGTGCGG
2221 TGGTTGAACT GCACACCGCC GACGGCACGC TGATTGAAGC AGAAGCCTGC GATGTCGGTT
2281 TCCGCGAGGT GCGGATTGAA AATGGTCTGC TGCTGCTGAA CGGCAAGCCG TTGCTGATTC
2341 GAGGCGTTAA CCGTCACGAG CATCATCCTC TGCATGGTCA GGTCATGGAT GAGCAGACGA
2401 TGGTGCAGGA TATCCTGCTG ATGAAGCAGA ACAACTTTAA CGCCGTGCGC TGTTCGCATT
2461 ATCCGAACCA TCCGCTGTGG TACACGCTGT GCGACCGCTA CGGCCTGTAT GTGGTGGATG
2521 AAGCCAATAT TGAAACCCAC GGCATGGTGC CAATGAATCG TCTGACCGAT GATCCGCGCT
2581 GGCTACCGGC GATGAGCGAA CGCGTAACGC GAATGGTGCA GCGCGATCGT AATCACCCGA
2641 GTGTGATCAT CTGGTCGCTG GGGAATGAAT CAGGCCACGG CGCTAATCAC GACGCGCTGT
2701 ATCGCTGGAT CAAATCTGTC GATCCTTCCC GCCCGGTGCA GTATGAAGGC GGCGGAGCCG
2761 ACACCACGGC CACCGATATT ATTTGCCCGA TGTACGCGCG CGTGGATGAA GACCAGCCCT
2821 TCCCGGCTGT GCCGAAATGG TCCATCAAAA AATGGCTTTC GCTACCTGGA GAGACGCGCC
2881 CGCTGATCCT TTGCGAATAC GCCCACGCGA TGGGTAACAG TCTTGGCGGT TTCGCTAAAT
2941 ACTGGCAGGC GTTTCGTCAG TATCCCCGTT TACAGGGCGG CTTCGTCTGG GACTGGGTGG
3001 ATCAGTCGCT GATTAAATAT GATGAAAACG GCAACCCGTG GTCGGCTTAC GGCGGTGATT
3061 TTGGCGATAC GCCGAACGAT CGCCAGTTCT GTATGAACGG TCTGGTCTTT GCCGACCGCA
3121 CGCCGCATCC AGCGCTGACG GAAGCAAAAC ACCAGCAGCA GTTTTTCCAG TTCCGTTTAT
3181 CCGGGCAAAC CATCGAAGTG ACCAGCGAAT ACCTGTTCCG TCATAGCGAT AACGAGCTCC
3241 TGCACTGGAT GGTGGCGCTG GATGGTAAGC CGCTGGCAAG CGGTGAAGTG CCTCTGGATG
3301 TCGCTCCACA AGGTAAACAG TTGATTGAAC TGCCTGAACT ACCGCAGCCG GAGAGCGCCG
3361 GGCAACTCTG GCTCACAGTA CGCGTAGTGC AACCGAACGC GACCGCATGG TCAGAAGCCG
3421 GGCACATCAG CGCCTGGCAG CAGTGGCGTC TGGCGGAAAA CCTCAGTGTG ACGCTCCCCG
3481 CCGCGTCCCA CGCCATCCCG CATCTGACCA CCAGCGAAAT GGATTTTTGC ATCGAGCTGG
3541 GTAATAAGCG TTGGCAATTT AACCGCCAGT CAGGCTTTCT TTCACAGATG TGGATTGGCG
3601 ATAAAAAACA ACTGCTGACG CCGCTGCGCG ATCAGTTCAC CCGTGCACCG CTGGATAACG
3661 ACATTGGCGT AAGTGAAGCG ACCCGCATTG ACCCTAACGC CTGGGTCGAA CGCTGGAAGG
3721 CGGCGGGCCA TTACCAGGCC GAAGCAGCGT TGTTGCAGTG CACGGCAGAT ACACTTGCTG
3781 ATGCGGTGCT GATTACGACC GCTCACGCGT GGCAGCATCA GGGGAAAACC TTATTTATCA
3841 GCCGGAAAAC CTACCGGATT GATGGTAGTG GTCAAATGGC GATTACCGTT GATGTTGAAG
3901 TGGCGAGCGA TACACCGCAT CCGGCGCGGA TTGGCCTGAA CTGCCAGCTG GCGCAGGTAG
3961 CAGAGCGGGT AAACTGGCTC GGATTAGGGC CGCAAGAAAA CTATCCCGAC CGCCTTACTG
4021 CCGCCTGTTT TGACCGCTGG GATCTGCCAT TGTCAGACAT GTATACCCCG TACGTCTTCC
4081 CGAGCGAAAA CGGTCTGCGC TGCGGGACGC GCGAATTGAA TTATGGCCCA CACCAGTGGC
4141 GCGGCGACTT CCAGTTCAAC ATCAGCCGCT ACAGTCAACA GCAACTGATG GAAACCAGCC
4201 ATCGCCATCT GCTGCACGCG GAAGAAGGCA CATGGCTGAA TATCGACGGT TTCCATATGG
4261 GGATTGGTGG CGACGACTCC TGGAGCCCGT CAGTATCGGC GGAATTCCAG CTGAGCGCCG
4321 GTCGCTACCA TTACCAGTTG GTCTGGTGTC AAAAATAATA ATAACCGGGC AGGCCATGTC
4381 TGCCCGTATT TCGCGTAAGG AAATCCATTA TGTACTATTT AAAAAACACA AACTTTTGGA
4441 TGTTCGGTTT ATTCTTTTTC TTTTACTTTT TTATCATGGG AGCCTACTTC CCGTTTTTCC
4501 CGATTTGGCT ACATGACATC AACCATATCA GCAAAAGTGA TACGGGTATT ATTTTTGCCG
4561 CTATTTCTCT GTTCTCGCTA TTATTCCAAC CGCTGTTTGG TCTGCTTTCT GACAAACTCG
4621 GGCTGCGCAA ATACCTGCTG TGGATTATTA CCGGCATGTT AGTGATGTTT GCGCCGTTCT
4681 TTATTTTTAT CTTCGGGCCA CTGTTACAAT ACAACATTTT AGTAGGATCG ATTGTTGGTG
4741 GTATTTATCT AGGCTTTTGT TTTAACGCCG GTGCGCCAGC AGTAGAGGCA TTTATTGAGA
4801 AAGTCAGCCG TCGCAGTAAT TTCGAATTTG GTCGCGCGCG GATGTTTGGC TGTGTTGGCT
4861 GGGCGCTGTG TGCCTCGATT GTCGGCATCA TGTTCACCAT CAATAATCAG TTTGTTTTCT
4921 GGCTGGGCTC TGGCTGTGCA CTCATCCTCG CCGTTTTACT CTTTTTCGCC AAAACGGATG
4981 CGCCCTCTTC TGCCACGGTT GCCAATGCGG TAGGTGCCAA CCATTCGGCA TTTAGCCTTA
5041 AGCTGGCACT GGAACTGTTC AGACAGCCAA AACTGTGGTT TTTGTCACTG TATGTTATTG
5101 GCGTTTCCTG CACCTACGAT GTTTTTGACC AACAGTTTGC TAATTTCTTT ACTTCGTTCT
5161 TTGCTACCGG TGAACAGGGT ACGCGGGTAT TTGGCTACGT AACGACAATG GGCGAATTAC
5221 TTAACGCCTC GATTATGTTC TTTGCGCCAC TGATCATTAA TCGCATCGGT GGGAAAAACG
5281 CCCTGCTGCT GGCTGGCACT ATTATGTCTG TACGTATTAT TGGCTCATCG TTCGCCACCT
5341 CAGCGCTGGA AGTGGTTATT CTGAAAACGC TGCATATGTT TGAAGTACCG TTCCTGCTGG
5401 TGGGCTGCTT TAAATATATT ACCAGCCAGT TTGAAGTGCG TTTTTCAGCG ACGATTTATC
5461 TGGTCTGTTT CTGCTTCTTT AAGCAACTGG CGATGATTTT TATGTCTGTA CTGGCGGGCA
5521 ATATGTATGA AAGCATCGGT TTCCAGGGCG CTTATCTGGT GCTGGGTCTG GTGGCGCTGG
5581 GCTTCACCTT AATTTCCGTG TTCACGCTTA GCGGCCCCGG CCCGCTTTCC CTGCTGCGTC
5641 GTCAGGTGAA TGAAGTCGCT TAAGCAATCA ATGTCGGATG CGGCGCGACG CTTATCCGAC
5701 CAACATATCA TAACGGAGTG ATCGCATTGA ACATGCCAAT GACCGAAAGA ATAAGAGCAG
5761 GCAAGCTATT TACCGATATG TGCGAAGGCT TACCGGAAAA AAGACTTCGT GGGAAAACGT
5821 TAATGTATGA GTTTAATCAC TCGCATCCAT CAGAAGTTGA AAAAAGAGAA AGCCTGATTA
5881 AAGAAATGTT TGCCACGGTA GGGGAAAACG CCTGGGTAGA ACCGCCTGTC TATTTCTCTT
5941 ACGGTTCCAA CATCCATATA GGCCGCAATT TTTATGCAAA TTTCAATTTA ACCATTGTCG
6001 ATGACTACAC GGTAACAATC GGTGATAACG TACTGATTGC ACCCAACGTT ACTCTTTCCG
6061 TTACGGGACA CCCTGTACAC CATGAATTGA GAAAAAACGG CGAGATGTAC TCTTTTCCGA
6121 TAACGATTGG CAATAACGTC TGGATCGGAA GTCATGTGGT TATTAATCCA GGCGTCACCA
6181 TCGGGGATAA TTCTGTTATT GGCGCGGGTA GTATCGTCAC AAAAGACATT CCACCAAACG
6241 TCGTGGCGGC TGGCGTTCCT TGTCGGGTTA TTCGCGAAAT AAACGACCGG GATAAGCACT
6301 ATTATTTCAA AGATTATAAA GTTGAATCGT CAGTTTAAAT TATAAAAATT GCCTGATACG
6361 CTGCGCTTAT CAGGCCTACA AGTTCAGCGA TCTACATTAG CCGCATCCGG CATGAACAAA
6421 GCGCAGGAAC AAGCGTCGCA TCATGCCTCT TTGACCCACA GCTGCGGAAA ACGTACTGGT
6481 GCAAAACGCA GGGTTATGAT CATCAGCCCA ACGACGCACA GCGCATGAAA TGCCCAGTCC
6541 ATCAGGTAAT TGCCGCTGAT ACTACGCAGC ACGCCAGAAA ACCACGGGGC AAGCCCGGCG
6601 ATGATAAAAC CGATTCCCTG CATAAACGCC ACCAGCTTGC CAGCAATAGC CGGTTGCACA
6661 GAGTGATCGA GCGCCAGCAG CAAACAGAGC GGAAACGCGC CGCCCAGACC TAACCCACAC
6721 ACCATCGCCC ACAATACCGG CAATTGCATC GGCAGCCAGA TAAAGCCGCA GAACCCCACC
6781 AGTTGTAACA CCAGCGCCAG CATTAACAGT TTGCGCCGAT CCTGATGGCG AGCCATAGCA
6841 GGCATCAGCA AAGCTCCTGC GGCTTGCCCA AGCGTCATCA ATGCCAGTAA GGAACCGCTG
6901 TACTGCGCGC TGGCACCAAT CTCAATATAG AAAGCGGGTA ACCAGGCAAT CAGGCTGGCG
6961 TAACCGCCGT TAATCAGACC GAAGTAAACA CCCAGCGTCC ACGCGCGGGG AGTGAATACC
7021 ACGCGAACCG GAGTGGTTGT TGTCTTGTGG GAAGAGGCGA CCTCGCGGGC GCTTTGCCAC
7081 CACCAGGCAA AGAGCGCAAC AACGGCAGGC AGCGCCACCA GGCGAGTGTT TGATACCAGG
7141 TTTCGCTATG TTGAACTAAC CAGGGCGTTA TGGCGGCACC AAGCCCACCG CCGCCCATCA
7201 GAGCCGCGGA CCACAGCCCC ATCACCAGTG GCGTGCGCTG CTGAAACCGC CGTTTAATCA
7261 CCGAAGCATC ACCGCCTGAA TGATGCCGAT CCCCACCCCA CCAAGCAGTG CGCTGCTAAG
7321 CAGCAGCGCA CTTTGCGGGT AAAGCTCACG CATCAATGCA CCGACGGCAA TCAGCAACAG
7381 ACTGATGGCG ACACTGCGAC GTTCGCTGAC ATGCTGATGA AGCCAGCTTC CGGCCAGCGC
7441 CAGCCCGCCC ATGGTAACCA CCGGCAGAGC GGTCGAC
//
LOCUS X51872 1832 bp DNA linear BCT 05-JUL-1999
DEFINITION Escherichia coli lacA gene for thiogalactoside transacetylase.
ACCESSION X51872
VERSION X51872.1 GI:41891
KEYWORDS lac operon; lacA gene; lacY gene; thiogalactoside transacetylase.
SOURCE Escherichia coli
ORGANISM Escherichia coli
Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
Enterobacteriaceae; Escherichia.
REFERENCE 1 (bases 1 to 1832)
AUTHORS Hediger,M.A., Johnson,D.F., Nierlich,D.P. and Zabin,I.
TITLE DNA sequence of the lactose operon: the lacA gene and the
transcriptional termination region
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 82 (19), 6414-6418 (1985)
PUBMED 3901000
REMARK (1-1832)
FEATURES Location/Qualifiers
source 1. .1832
/organism="Escherichia coli"
/mol_type="genomic DNA"
/db_xref="taxon:562"
CDS <1. .18
/codon_start=1
/transl_table=11
/product="lacY gene product"
/protein_id="CAA36161.1"
/db_xref="GI:4377496"
/translation="VNEVA"
gene 82. .693
/gene="lacA"
CDS 82. .693
/gene="lacA"
/codon_start=1
/transl_table=11
/product="thiogalactoside transacetylase"
/protein_id="CAA36162.1"
/db_xref="GI:581122"
/db_xref="GOA:P07464"
/db_xref="InterPro:IPR001451"
/db_xref="InterPro:IPR011004"
/db_xref="PDB:1KQA"
/db_xref="PDB:1KRR"
/db_xref="PDB:1KRU"
/db_xref="PDB:1KRV"
/db_xref="UniProtKB/Swiss-Prot:P07464"
/translation="MNMPMTERIRAGKLFTDMCEGLPEKRLRGKTLMYEFNHSHPSEV
EKRESLIKEMFATVGENAWVEPPVYFSYGSNIHIGRNFYANFNLTIVDDYTVTIGDNV
LIAPNVTLSVTGHPVHHELRKNGEMYSFPITIGNNVWIGSHVVINPGVTIGDNSVIGA
GSIVTKDIPPNVVAAGVPCRVIREINDRDKHYYFKDYKVESSV"
ORIGIN
1 GTGAATGAAG TCGCTTAAGC AATCAATGTC GGATGCGGCG CGACGCTTAT CCGACCAACA
61 TATCATAACG GAGTGATCGC ATTGAACATG CCAATGACCG AAAGAATAAG AGCAGGCAAG
121 CTATTTACCG ATATGTGCGA AGGCTTACCG GAAAAAAGAC TTCGTGGGAA AACGTTAATG
181 TATGAGTTTA ATCACTCGCA TCCATCAGAA GTTGAAAAAA GAGAAAGCCT GATTAAAGAA
241 ATGTTTGCCA CGGTAGGGGA AAACGCCTGG GTAGAACCGC CTGTCTATTT CTCTTACGGT
301 TCCAACATCC ATATAGGCCG CAATTTTTAT GCAAATTTCA ATTTAACCAT TGTCGATGAC
361 TACACGGTAA CAATCGGTGA TAACGTACTG ATTGCACCCA ACGTTACTCT TTCCGTTACG
421 GGACACCCTG TACACCATGA ATTGAGAAAA AACGGCGAGA TGTACTCTTT TCCGATAACG
481 ATTGGCAATA ACGTCTGGAT CGGAAGTCAT GTGGTTATTA ATCCAGGCGT CACCATCGGG
541 GATAATTCTG TTATTGGCGC GGGTAGTATC GTCACAAAAG ACATTCCACC AAACGTCGTG
601 GCGGCTGGCG TTCCTTGTCG GGTTATTCGC GAAATAAACG ACCGGGATAA GCACTATTAT
661 TTCAAAGATT ATAAAGTTGA ATCGTCAGTT TAAATTATAA AAATTGCCTG ATACGCTGCG
721 CTTATCAGGC CTACAAGTTC AGCGATCTAC ATTAGCCGCA TCCGGCATGA ACAAAGCGCA
781 GGAACAAGCG TCGCATCATG CCTCTTTGAC CCACAGCTGC GGAAAACGTA CTGGTGCAAA
841 ACGCAGGGTT ATGATCATCA GCCCAACGAC GCACAGCGCA TGAAATGCCC AGTCCATCAG
901 GTAATTGCCG CTGATACTAC GCAGCACGCC AGAAAACCAC GGGGCAAGCC CGGCGATGAT
961 AAAACCGATT CCCTGCATAA ACGCCACCAG CTTGCCAGCA ATAGCCGGTT GCACAGAGTG
1021 ATCGAGCGCC AGCAGCAAAC AGAGCGGAAA CGCGCCGCCC AGACCTAACC CACACACCAT
1081 CGCCCACAAT ACCGGCAATT GCATCGGCAG CCAGATAAAG CCGCAGAACC CCACCAGTTG
1141 TAACACCAGC GCCAGCATTA ACAGTTTGCG CCGATCCTGA TGGCGAGCCA TAGCAGGCAT
1201 CAGCAAAGCT CCTGCGGCTT GCCCAAGCGT CATCAATGCC AGTAAGGAAC CGCTGTACTG
1261 CGCGCTGGCA CCAATCTCAA TATAGAAAGC GGGTAACCAG GCAATCAGGC TGGCGTAACC
1321 GCCGTTAATC AGACCGAAGT AAACACCCAG CGTCCACGCG CGGGGAGTGA ATACCACGCG
1381 AACCGGAGTG GTTGTTGTCT TGTGGGAAGA GGCGACCTCG CGGGCGCTTT GCCACCACCA
1441 GGCAAAGAGC GCAACAACGG CAGGCAGCGC CACCAGGCGA GTGTTTGATA CCAGGTTTCG
1501 CTATGTTGAA CTAACCAGGG CGTTATGGCG GCACCAAGCC CACCGCCGCC CATCAGAGCC
1561 GCGGACCACA GCCCCATCAC CAGTGGCGTG CGCTGCTGAA ACCGCCGTTT AATCACCGAA
1621 GCATCACCGC CTGAATGATG CCGATCCCCA CCCCACCAAG CAGTGCGCTG CTAAGCAGCA
1681 GCGCACTTTG CGGGTAAAGC TCACGCATCA ATGCACCGAC GGCAATCAGC AACAGACTGA
1741 TGGCGACACT GCGACGTTCG CTGACATGCT GATGAAGCCA GCTTCCGGCC AGCGCCAGCC
1801 CGCCCATGGT AACCACCGGC AGAGCGGTCG AC
//
LOCUS V00294 1113 bp DNA linear BCT 10-FEB-1999
DEFINITION E. coli laci gene (codes for the lac repressor).
ACCESSION V00294
VERSION V00294.1 GI:41893
KEYWORDS DNA binding protein; repressor.
SOURCE Escherichia coli
ORGANISM Escherichia coli
Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
Enterobacteriaceae; Escherichia.
REFERENCE 1 (bases 1 to 1113)
AUTHORS Farabaugh,P.J.
TITLE Sequence of the lacI gene
JOURNAL Nature 274 (5673), 765-769 (1978)
PUBMED 355891
COMMENT KST ECO.LACI.
FEATURES Location/Qualifiers
source 1. .1113
/organism="Escherichia coli"
/mol_type="genomic DNA"
/db_xref="taxon:562"
CDS 31. .1113
/note="unnamed protein product; reading frame"
/codon_start=1
/transl_table=11
/protein_id="CAA23569.1"
/db_xref="GI:581123"
/db_xref="GOA:P03023"
/db_xref="PDB:1CJG"
/db_xref="PDB:1EFA"
/db_xref="PDB:1JWL"
/db_xref="PDB:1JYE"
/db_xref="PDB:1JYF"
/db_xref="PDB:1L1M"
/db_xref="PDB:1LBG"
/db_xref="PDB:1LBH"
/db_xref="PDB:1LBI"
/db_xref="PDB:1LCC"
/db_xref="PDB:1LCD"
/db_xref="PDB:1LQC"
/db_xref="PDB:1LTP"
/db_xref="PDB:1TLF"
/db_xref="PDB:2BJC"
/db_xref="UniProtKB/Swiss-Prot:P03023"
/translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAE
LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS
GVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSII
FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREG
DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDT
EDSSCYIPPSTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNT
QTASPRALADSLMQLARQVSRLESGQ"
ORIGIN
1 CCGGAAGAGA GTCAATTCAG GGTGGTGAAT GTGAAACCAG TAACGTTATA CGATGTCGCA
61 GAGTATGCCG GTGTCTCTTA TCAGACCGTT TCCCGCGTGG TGAACCAGGC CAGCCACGTT
121 TCTGCGAAAA CGCGGGAAAA AGTGGAAGCG GCGATGGCGG AGCTGAATTA CATTCCCAAC
181 CGCGTGGCAC AACAACTGGC GGGCAAACAG TCGTTGCTGA TTGGCGTTGC CACCTCCAGT
241 CTGGCCCTGC ACGCGCCGTC GCAAATTGTC GCGGCGATTA AATCTCGCGC CGATCAACTG
301 GGTGCCAGCG TGGTGGTGTC GATGGTAGAA CGAAGCGGCG TCGAAGCCTG TAAAGCGGCG
361 GTGCACAATC TTCTCGCGCA ACGCGTCAGT GGGCTGATCA TTAACTATCC GCTGGATGAC
421 CAGGATGCCA TTGCTGTGGA AGCTGCCTGC ACTAATGTTC CGGCGTTATT TCTTGATGTC
481 TCTGACCAGA CACCCATCAA CAGTATTATT TTCTCCCATG AAGACGGTAC GCGACTGGGC
541 GTGGAGCATC TGGTCGCATT GGGTCACCAG CAAATCGCGC TGTTAGCGGG CCCATTAAGT
601 TCTGTCTCGG CGCGTCTGCG TCTGGCTGGC TGGCATAAAT ATCTCACTCG CAATCAAATT
661 CAGCCGATAG CGGAACGGGA AGGCGACTGG AGTGCCATGT CCGGTTTTCA ACAAACCATG
721 CAAATGCTGA ATGAGGGCAT CGTTCCCACT GCGATGCTGG TTGCCAACGA TCAGATGGCG
781 CTGGGCGCAA TGCGCGCCAT TACCGAGTCC GGGCTGCGCG TTGGTGCGGA TATCTCGGTA
841 GTGGGATACG ACGATACCGA AGACAGCTCA TGTTATATCC CGCCGTCAAC CACCATCAAA
901 CAGGATTTTC GCCTGCTGGG GCAAACCAGC GTGGACCGCT TGCTGCAACT CTCTCAGGGC
961 CAGGCGGTGA AGGGCAATCA GCTGTTGCCC GTCTCACTGG TGAAAAGAAA AACCACCCTG
1021 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA
1081 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGA
//
LOCUS V00295 1500 bp DNA linear BCT 07-JUL-1995
DEFINITION E. coli lacY gene (codes for lactose permease).
ACCESSION V00295
VERSION V00295.1 GI:41897
KEYWORDS membrane protein.
SOURCE Escherichia coli
ORGANISM Escherichia coli
Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
Enterobacteriaceae; Escherichia.
REFERENCE 1 (bases 1 to 1500)
AUTHORS Buchel,D.E., Gronenborn,B. and Muller-Hill,B.
TITLE Sequence of the lactose permease gene
JOURNAL Nature 283 (5747), 541-545 (1980)
PUBMED 6444453
COMMENT lacZ is a beta-galactosidase and lacA is transacetylase. KST
ECO.LACY.
FEATURES Location/Qualifiers
source 1. .1500
/organism="Escherichia coli"
/mol_type="genomic DNA"
/db_xref="taxon:562"
CDS <1. .54
/note="unnamed protein product; reading frame (lacZ)"
/codon_start=1
/transl_table=11
/protein_id="CAA23570.1"
/db_xref="GI:41898"
/db_xref="GOA:P00722"
/db_xref="PDB:1BGL"
/db_xref="PDB:1BGM"
/db_xref="PDB:1DP0"
/db_xref="PDB:1F49"
/db_xref="PDB:1F4A"
/db_xref="PDB:1F4H"
/db_xref="PDB:1GHO"
/db_xref="PDB:1HN1"
/db_xref="PDB:1JYN"
/db_xref="PDB:1JYV"
/db_xref="PDB:1JYW"
/db_xref="PDB:1JYX"
/db_xref="PDB:1JYY"
/db_xref="PDB:1JYZ"
/db_xref="PDB:1JZ0"
/db_xref="PDB:1JZ1"
/db_xref="PDB:1JZ2"
/db_xref="PDB:1JZ3"
/db_xref="PDB:1JZ4"
/db_xref="PDB:1JZ5"
/db_xref="PDB:1JZ6"
/db_xref="PDB:1JZ7"
/db_xref="PDB:1JZ8"
/db_xref="PDB:1PX3"
/db_xref="PDB:1PX4"
/db_xref="UniProtKB/Swiss-Prot:P00722"
/translation="FQLSAGRYHYQLVWCQK"
CDS 106. .1359
/note="unnamed protein product; reading frame (lacY)"
/codon_start=1
/transl_table=11
/protein_id="CAA23571.1"
/db_xref="GI:41899"
/db_xref="GOA:P02920"
/db_xref="InterPro:IPR000576"
/db_xref="InterPro:IPR007114"
/db_xref="PDB:1M2U"
/db_xref="PDB:1PV6"
/db_xref="PDB:1PV7"
/db_xref="UniProtKB/Swiss-Prot:P02920"
/translation="MYYLKNTNFWMFGLFFFFYFFIMGAYFPFFPIWLHDINHISKSD
TGIIFAAISLFSLLFQPLFGLLSDKLGLRKYLLWIITGMLVMFAPFFIFIFGPLLQYN
ILVGSIVGGIYLGFCFNAGAPAVEAFIEKVSRRSNFEFGRARMFGCVGWALCASIVGI
MFTINNQFVFWLGSGCALILAVLLFFAKTDAPSSATVANAVGANHSAFSLKLALELFR
QPKLWFLSLYVIGVSCTYDVFDQQFANFFTSFFATGEQGTRVFGYVTTMGELLNASIM
FFAPLIINRIGGKNALLLAGTIMSVRIIGSSFATSALEVVILKTLHMFEVPFLLVGCF
KYITSQFEVRFSATIYLVCFCFFKQLAMIFMSVLAGNMYESIGFQGAYLVLGLVALGF
TLISVFTLSGPGPLSLLRRQVNEVA"
CDS 1423. .>1500
/note="unnamed protein product; reading frame (lacA)"
/codon_start=1
/transl_table=11
/protein_id="CAA23572.1"
/db_xref="GI:581124"
/db_xref="GOA:P07464"
/db_xref="PDB:1KQA"
/db_xref="PDB:1KRR"
/db_xref="PDB:1KRU"
/db_xref="PDB:1KRV"
/db_xref="UniProtKB/Swiss-Prot:P07464"
/translation="MNMPMTERIRAGKLFTDMCEGLPEKR"
ORIGIN
1 TTCCAGCTGA GCGCCGGTCG CTACCATTAC CAGTTGGTCT GGTGTCAAAA ATAATAATAA
61 CCGGGCAGGC CATGTCTGCC CGTATTTCGC GTAAGGAAAT CCATTATGTA CTATTTAAAA
121 AACACAAACT TTTGGATGTT CGGTTTATTC TTTTTCTTTT ACTTTTTTAT CATGGGAGCC
181 TACTTCCCGT TTTTCCCGAT TTGGCTACAT GACATCAACC ATATCAGCAA AAGTGATACG
241 GGTATTATTT TTGCCGCTAT TTCTCTGTTC TCGCTATTAT TCCAACCGCT GTTTGGTCTG
301 CTTTCTGACA AACTCGGGCT GCGCAAATAC CTGCTGTGGA TTATTACCGG CATGTTAGTG
361 ATGTTTGCGC CGTTCTTTAT TTTTATCTTC GGGCCACTGT TACAATACAA CATTTTAGTA
421 GGATCGATTG TTGGTGGTAT TTATCTAGGC TTTTGTTTTA ACGCCGGTGC GCCAGCAGTA
481 GAGGCATTTA TTGAGAAAGT CAGCCGTCGC AGTAATTTCG AATTTGGTCG CGCGCGGATG
541 TTTGGCTGTG TTGGCTGGGC GCTGTGTGCC TCGATTGTCG GCATCATGTT CACCATCAAT
601 AATCAGTTTG TTTTCTGGCT GGGCTCTGGC TGTGCACTCA TCCTCGCCGT TTTACTCTTT
661 TTCGCCAAAA CGGATGCGCC CTCTTCTGCC ACGGTTGCCA ATGCGGTAGG TGCCAACCAT
721 TCGGCATTTA GCCTTAAGCT GGCACTGGAA CTGTTCAGAC AGCCAAAACT GTGGTTTTTG
781 TCACTGTATG TTATTGGCGT TTCCTGCACC TACGATGTTT TTGACCAACA GTTTGCTAAT
841 TTCTTTACTT CGTTCTTTGC TACCGGTGAA CAGGGTACGC GGGTATTTGG CTACGTAACG
901 ACAATGGGCG AATTACTTAA CGCCTCGATT ATGTTCTTTG CGCCACTGAT CATTAATCGC
961 ATCGGTGGGA AAAACGCCCT GCTGCTGGCT GGCACTATTA TGTCTGTACG TATTATTGGC
1021 TCATCGTTCG CCACCTCAGC GCTGGAAGTG GTTATTCTGA AAACGCTGCA TATGTTTGAA
1081 GTACCGTTCC TGCTGGTGGG CTGCTTTAAA TATATTACCA GCCAGTTTGA AGTGCGTTTT
1141 TCAGCGACGA TTTATCTGGT CTGTTTCTGC TTCTTTAAGC AACTGGCGAT GATTTTTATG
1201 TCTGTACTGG CGGGCAATAT GTATGAAAGC ATCGGTTTCC AGGGCGCTTA TCTGGTGCTG
1261 GGTCTGGTGG CGCTGGGCTT CACCTTAATT TCCGTGTTCA CGCTTAGCGG CCCCGGCCCG
1321 CTTTCCCTGC TGCGTCGTCA GGTGAATGAA GTCGCTTAAG CAATCAATGT CGGATGCGGC
1381 GCGACGCTTA TCCGACCAAC ATATCATAAC GGAGTGATCG CATTGAACAT GCCAATGACC
1441 GAAAGAATAA GAGCAGGCAA GCTATTTACC GATATGTGCG AAGGCTTACC GGAAAAAAGA
//
LOCUS V00296 3078 bp DNA linear BCT 18-APR-2005
DEFINITION E. coli gene lacZ coding for beta-galactosidase (EC 3.2.1.23).
ACCESSION V00296
VERSION V00296.1 GI:41901
KEYWORDS galactosidase.
SOURCE Escherichia coli
ORGANISM Escherichia coli
Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
Enterobacteriaceae; Escherichia.
REFERENCE 1 (bases 1 to 3078)
AUTHORS Kalnins,A., Otto,K., Ruther,U. and Muller-Hill,B.
TITLE Sequence of the lacZ gene of Escherichia coli
JOURNAL EMBO J. 2 (4), 593-597 (1983)
PUBMED 6313347
REFERENCE 2
AUTHORS Zell,R. and Fritz,H.J.
TITLE DNA mismatch-repair in Escherichia coli counteracting the
hydrolytic deamination of 5-methyl-cytosine residues
JOURNAL EMBO J. 6 (6), 1809-1815 (1987)
PUBMED 3038536
COMMENT Data kindly reviewed (18-MAY-1983) by U. Ruether.
FEATURES Location/Qualifiers
source 1. .3078
/organism="Escherichia coli"
/mol_type="genomic DNA"
/db_xref="taxon:562"
CDS <1. .3072
/note="unnamed protein product; galactosidase"
/codon_start=1
/transl_table=11
/protein_id="CAA23573.1"
/db_xref="GI:1197203"
/db_xref="GOA:P00722"
/db_xref="InterPro:IPR004199"
/db_xref="InterPro:IPR006101"
/db_xref="InterPro:IPR006102"
/db_xref="InterPro:IPR006103"
/db_xref="InterPro:IPR006104"
/db_xref="InterPro:IPR008979"
/db_xref="InterPro:IPR011013"
/db_xref="PDB:1BGL"
/db_xref="PDB:1BGM"
/db_xref="PDB:1DP0"
/db_xref="PDB:1F49"
/db_xref="PDB:1F4A"
/db_xref="PDB:1F4H"
/db_xref="PDB:1GHO"
/db_xref="PDB:1HN1"
/db_xref="PDB:1JYN"
/db_xref="PDB:1JYV"
/db_xref="PDB:1JYW"
/db_xref="PDB:1JYX"
/db_xref="PDB:1JYY"
/db_xref="PDB:1JYZ"
/db_xref="PDB:1JZ0"
/db_xref="PDB:1JZ1"
/db_xref="PDB:1JZ2"
/db_xref="PDB:1JZ3"
/db_xref="PDB:1JZ4"
/db_xref="PDB:1JZ5"
/db_xref="PDB:1JZ6"
/db_xref="PDB:1JZ7"
/db_xref="PDB:1JZ8"
/db_xref="PDB:1PX3"
/db_xref="PDB:1PX4"
/db_xref="UniProtKB/Swiss-Prot:P00722"
/translation="TMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEART
DRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTN
VTYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVG
YGQDSRLPSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKP
TTQISDFHVATRFNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGG
EIIDERGGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFRE
VRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHY
PNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNH
PSVIIWSLGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDE
DQPFPAVPKWSIKKWLSLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGF
VWDWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQ
QFFQFRLSGQTIEVTSEYLFRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIEL
PELPQPESAGQLWLTVRVVQPNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHL
TTSEMDFCIELGNKRWQFNRQSGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEA
TRIDPNAWVERWKAAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTY
RIDGSGQMAITVDVEVASDTPHPARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAAC
FDRWDLPLSDMYTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETSH
RHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQK"
mat_peptide 1. .3069
/product="unnamed"
ORIGIN
1 ACCATGATTA CGGATTCACT GGCCGTCGTT TTACAACGTC GTGACTGGGA AAACCCTGGC
61 GTTACCCAAC TTAATCGCCT TGCAGCACAT CCCCCTTTCG CCAGCTGGCG TAATAGCGAA
121 GAGGCCCGCA CCGATCGCCC TTCCCAACAG TTGCGCAGCC TGAATGGCGA ATGGCGCTTT
181 GCCTGGTTTC CGGCACCAGA AGCGGTGCCG GAAAGCTGGC TGGAGTGCGA TCTTCCTGAG
241 GCCGATACTG TCGTCGTCCC CTCAAACTGG CAGATGCACG GTTACGATGC GCCCATCTAC
301 ACCAACGTAA CCTATCCCAT TACGGTCAAT CCGCCGTTTG TTCCCACGGA GAATCCGACG
361 GGTTGTTACT CGCTCACATT TAATGTTGAT GAAAGCTGGC TACAGGAAGG CCAGACGCGA
421 ATTATTTTTG ATGGCGTTAA CTCGGCGTTT CATCTGTGGT GCAACGGGCG CTGGGTCGGT
481 TACGGCCAGG ACAGTCGTTT GCCGTCTGAA TTTGACCTGA GCGCATTTTT ACGCGCCGGA
541 GAAAACCGCC TCGCGGTGAT GGTGCTGCGT TGGAGTGACG GCAGTTATCT GGAAGATCAG
601 GATATGTGGC GGATGAGCGG CATTTTCCGT GACGTCTCGT TGCTGCATAA ACCGACTACA
661 CAAATCAGCG ATTTCCATGT TGCCACTCGC TTTAATGATG ATTTCAGCCG CGCTGTACTG
721 GAGGCTGAAG TTCAGATGTG CGGCGAGTTG CGTGACTACC TACGGGTAAC AGTTTCTTTA
781 TGGCAGGGTG AAACGCAGGT CGCCAGCGGC ACCGCGCCTT TCGGCGGTGA AATTATCGAT
841 GAGCGTGGTG GTTATGCCGA TCGCGTCACA CTACGTCTGA ACGTCGAAAA CCCGAAACTG
901 TGGAGCGCCG AAATCCCGAA TCTCTATCGT GCGGTGGTTG AACTGCACAC CGCCGACGGC
961 ACGCTGATTG AAGCAGAAGC CTGCGATGTC GGTTTCCGCG AGGTGCGGAT TGAAAATGGT
1021 CTGCTGCTGC TGAACGGCAA GCCGTTGCTG ATTCGAGGCG TTAACCGTCA CGAGCATCAT
1081 CCTCTGCATG GTCAGGTCAT GGATGAGCAG ACGATGGTGC AGGATATCCT GCTGATGAAG
1141 CAGAACAACT TTAACGCCGT GCGCTGTTCG CATTATCCGA ACCATCCGCT GTGGTACACG
1201 CTGTGCGACC GCTACGGCCT GTATGTGGTG GATGAAGCCA ATATTGAAAC CCACGGCATG
1261 GTGCCAATGA ATCGTCTGAC CGATGATCCG CGCTGGCTAC CGGCGATGAG CGAACGCGTA
1321 ACGCGAATGG TGCAGCGCGA TCGTAATCAC CCGAGTGTGA TCATCTGGTC GCTGGGGAAT
1381 GAATCAGGCC ACGGCGCTAA TCACGACGCG CTGTATCGCT GGATCAAATC TGTCGATCCT
1441 TCCCGCCCGG TGCAGTATGA AGGCGGCGGA GCCGACACCA CGGCCACCGA TATTATTTGC
1501 CCGATGTACG CGCGCGTGGA TGAAGACCAG CCCTTCCCGG CTGTGCCGAA ATGGTCCATC
1561 AAAAAATGGC TTTCGCTACC TGGAGAGACG CGCCCGCTGA TCCTTTGCGA ATACGCCCAC
1621 GCGATGGGTA ACAGTCTTGG CGGTTTCGCT AAATACTGGC AGGCGTTTCG TCAGTATCCC
1681 CGTTTACAGG GCGGCTTCGT CTGGGACTGG GTGGATCAGT CGCTGATTAA ATATGATGAA
1741 AACGGCAACC CGTGGTCGGC TTACGGCGGT GATTTTGGCG ATACGCCGAA CGATCGCCAG
1801 TTCTGTATGA ACGGTCTGGT CTTTGCCGAC CGCACGCCGC ATCCAGCGCT GACGGAAGCA
1861 AAACACCAGC AGCAGTTTTT CCAGTTCCGT TTATCCGGGC AAACCATCGA AGTGACCAGC
1921 GAATACCTGT TCCGTCATAG CGATAACGAG CTCCTGCACT GGATGGTGGC GCTGGATGGT
1981 AAGCCGCTGG CAAGCGGTGA AGTGCCTCTG GATGTCGCTC CACAAGGTAA ACAGTTGATT
2041 GAACTGCCTG AACTACCGCA GCCGGAGAGC GCCGGGCAAC TCTGGCTCAC AGTACGCGTA
2101 GTGCAACCGA ACGCGACCGC ATGGTCAGAA GCCGGGCACA TCAGCGCCTG GCAGCAGTGG
2161 CGTCTGGCGG AAAACCTCAG TGTGACGCTC CCCGCCGCGT CCCACGCCAT CCCGCATCTG
2221 ACCACCAGCG AAATGGATTT TTGCATCGAG CTGGGTAATA AGCGTTGGCA ATTTAACCGC
2281 CAGTCAGGCT TTCTTTCACA GATGTGGATT GGCGATAAAA AACAACTGCT GACGCCGCTG
2341 CGCGATCAGT TCACCCGTGC ACCGCTGGAT AACGACATTG GCGTAAGTGA AGCGACCCGC
2401 ATTGACCCTA ACGCCTGGGT CGAACGCTGG AAGGCGGCGG GCCATTACCA GGCCGAAGCA
2461 GCGTTGTTGC AGTGCACGGC AGATACACTT GCTGATGCGG TGCTGATTAC GACCGCTCAC
2521 GCGTGGCAGC ATCAGGGGAA AACCTTATTT ATCAGCCGGA AAACCTACCG GATTGATGGT
2581 AGTGGTCAAA TGGCGATTAC CGTTGATGTT GAAGTGGCGA GCGATACACC GCATCCGGCG
2641 CGGATTGGCC TGAACTGCCA GCTGGCGCAG GTAGCAGAGC GGGTAAACTG GCTCGGATTA
2701 GGGCCGCAAG AAAACTATCC CGACCGCCTT ACTGCCGCCT GTTTTGACCG CTGGGATCTG
2761 CCATTGTCAG ACATGTATAC CCCGTACGTC TTCCCGAGCG AAAACGGTCT GCGCTGCGGG
2821 ACGCGCGAAT TGAATTATGG CCCACACCAG TGGCGCGGCG ACTTCCAGTT CAACATCAGC
2881 CGCTACAGTC AACAGCAACT GATGGAAACC AGCCATCGCC ATCTGCTGCA CGCGGAAGAA
2941 GGCACATGGC TGAATATCGA CGGTTTCCAT ATGGGGATTG GTGGCGACGA CTCCTGGAGC
3001 CCGTCAGTAT CGGCGGAATT CCAGCTGAGC GCCGGTCGCT ACCATTACCA GTTGGTCTGG
3061 TGTCAAAAAT AATAATAA
//
LOCUS X77160 1212 bp DNA linear BCT 18-APR-2005
DEFINITION Pseudomonas aeruginosa (PAC1) amiB gene.
ACCESSION X77160
VERSION X77160.1 GI:473108
KEYWORDS amiB gene.
SOURCE Pseudomonas aeruginosa
ORGANISM Pseudomonas aeruginosa
Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
Pseudomonadaceae; Pseudomonas.
REFERENCE 1 (bases 1 to 1212)
AUTHORS Wilson,S.A., Williams,R.J., Pearl,L.H. and Drew,R.E.
TITLE Identification of two new genes in the Pseudomonas aeruginosa
amidase operon, encoding an ATPase (AmiB) and a putative integral
membrane protein (AmiS)
JOURNAL J. Biol. Chem. 270 (32), 18818-18824 (1995)
PUBMED 7642533
REFERENCE 2
AUTHORS Wilson,S.A., Wachira,S.M., Drew,R.E., Jones,D. and Pearl,L.H.
TITLE Identification of a putative ABC transporter in the Pseudomonas
aeruginosa amidase operon
JOURNAL Unpublished
REFERENCE 3 (bases 1 to 1212)
AUTHORS Wilson,S.A.
TITLE Direct Submission
JOURNAL Submitted (17-JAN-1994) S.A. Wilson, University College London,
Dept of Biochemistry, Gower Street, London WC1E 6BT, UK
FEATURES Location/Qualifiers
source 1. .1212
/organism="Pseudomonas aeruginosa"
/mol_type="genomic DNA"
/strain="PAC1"
/db_xref="taxon:287"
/clone="plasmid pAS20"
gene 97. .1212
/gene="amiB"
CDS 97. .1212
/gene="amiB"
/codon_start=1
/transl_table=11
/protein_id="CAA54405.1"
/db_xref="GI:473109"
/db_xref="GOA:Q51416"
/db_xref="UniProtKB/Swiss-Prot:Q51416"
/translation="MPFLSDMLDQSRRQQDEEQALARENLAEASLLQAHLSHRSALHS
RFRFDPAAVMDCLRAEVLGQEPALQAVEDMLKVVRADIADPRRPLFSALFLGPTGVGK
TEIVRALARALHGDAEGFCRVDMNTLSQEHYAAALTGAPPGYVGAKEGTTLLEQDKLD
GSPGRPGIVLFDELEKASPEVVHALLNVLDNGLLRVASGERTYHFRNTLVFMTSNLCA
HEIQRYDERRQRLPWRLLPVGGERRRRDIDGMVRARLLKTFSPEFVNRLDSVVTFNWI
ERDVVARLVELEVQRLNRRLEKHRCRLEATPEVLAKIARAGFDRQFGARALRRSVRHH
LEVPLAEHLLDHHQPGDGNCTIYLASLEHERVRFVRR"
ORIGIN
1 GAAGGAAGCC TGACGGCAGA CGCCGCCAGC CCGGCGTGCC GTCGTGCGGC ACGCCGTCTC
61 CGCCATTTCC CCCCTGTGGC AGAAGGAGTT TCATCCATGC CTTTTCTGAG CGACATGCTC
121 GACCAGTCCC GCCGGCAGCA GGACGAGGAA CAGGCCCTGG CGCGGGAGAA TCTTGCCGAG
181 GCAAGTCTGC TCCAGGCCCA CCTGAGTCAC CGCAGCGCCC TGCACAGCCG TTTCCGTTTC
241 GACCCGGCGG CGGTGATGGA CTGCCTGCGC GCCGAGGTGC TCGGCCAGGA ACCGGCGCTA
301 CAGGCCGTCG AGGACATGCT CAAGGTGGTT CGCGCGGACA TCGCCGACCC GCGCCGTCCG
361 CTGTTCAGCG CGCTGTTCCT CGGCCCCACC GGAGTCGGCA AGACCGAGAT CGTGCGCGCC
421 CTGGCCAGGG CCCTGCACGG CGACGCCGAG GGGTTCTGCC GGGTGGACAT GAACACCCTG
481 TCCCAGGAGC ACTATGCCGC CGCCCTCACC GGTGCGCCGC CGGGCTACGT CGGGGCGAAG
541 GAGGGCACCA CCCTGTTGGA GCAGGACAAG CTGGACGGCA GTCCCGGGCG CCCCGGCATC
601 GTTCTCTTCG ACGAACTGGA AAAGGCCAGC CCGGAAGTGG TCCATGCGTT GCTCAACGTA
661 CTCGACAACG GCCTGCTACG GGTCGCTTCC GGCGAACGCA CCTACCATTT CCGCAACACC
721 CTGGTGTTCA TGACCAGCAA TCTCTGCGCC CATGAGATCC AGCGCTACGA CGAGCGTCGC
781 CAGCGCCTGC CCTGGCGCCT GCTGCCGGTC GGCGGCGAGC GCCGGCGGCG GGACATCGAC
841 GGGATGGTCC GGGCCCGGCT GCTGAAGACC TTCTCGCCGG AGTTCGTCAA TCGTCTCGAT
901 AGCGTGGTCA CCTTCAACTG GATCGAACGC GACGTCGTCG CGCGCCTGGT CGAGCTGGAG
961 GTGCAGCGGC TCAACCGGCG CCTGGAGAAG CATCGCTGCC GCCTGGAGGC GACCCCGGAG
1021 GTGCTGGCGA AGATCGCCCG CGCCGGCTTC GACCGGCAGT TCGGCGCCCG TGCGTTGCGC
1081 CGCAGCGTCC GTCATCATCT CGAGGTACCG CTGGCCGAGC ATCTGCTCGA TCACCACCAG
1141 CCGGGCGACG GGAACTGCAC GATCTACCTG GCGAGCCTGG AGCACGAGCG GGTTCGCTTC
1201 GTACGGCGCT GA
//
LOCUS M27612 1065 bp DNA linear BCT 19-APR-2002
DEFINITION Pseudomonas aeruginosa aliphatic amidase (amiE) gene, complete cds.
ACCESSION M27612
VERSION M27612.1 GI:150979
KEYWORDS .
SOURCE Pseudomonas aeruginosa
ORGANISM Pseudomonas aeruginosa
Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
Pseudomonadaceae; Pseudomonas.
REFERENCE 1 (bases 1 to 1065)
AUTHORS Brammar,W.J., Charles,I.G., Matfield,M., Liu,C.P., Drew,R.E. and
Clarke,P.H.
TITLE The nucleotide sequence of the amiE gene of Pseudomonas aeruginosa
JOURNAL FEBS Lett. 215 (2), 291-294 (1987)
PUBMED 3108030
FEATURES Location/Qualifiers
source 1. .1065
/organism="Pseudomonas aeruginosa"
/mol_type="genomic DNA"
/strain="PAC433"
/db_xref="taxon:287"
gene 25. .1065
/gene="amiE"
CDS 25. .1065
/gene="amiE"
/EC_number="3.5.1.4"
/codon_start=1
/transl_table=11
/product="aliphatic amidase"
/protein_id="AAA25697.1"
/db_xref="GI:150980"
/translation="MRHGDISSSNDTVGVAVVNYKMPRLHTAAEVLDNARKIADMIVG
MKQGLPGMDLVVFPEYSLQGIMYDPAEMMETAVAIPGEETEIFSRACRKANVWGVFSL
TGERHEEHPRKAPYNTLVLIDNNGEIVQKYRKIIPWCPIEGWYPGGQTYVSEGPKGMK
ISLIICDDPNYPEIWRDCAMKGAELIVRCQGYMYPAKDQQVMMAKAMAWANNCYVAVA
NAAGFDGVYSYFGHSAIIGFDGRTLGECGEEEMGIQYAQLSLSQIRDARANDQSQNHL
FKILHRGYSGLQASGDGDRGLAECPFEFYRTWVTDAEKARDNVERLTRSTTGVAQCPV
GRLPYEGLEKEA"
ORIGIN
1 AAATAACAAC AGGAGGTGAT ACCCATGCGT CACGGCGATA TTTCCAGCAG CAACGACACC
61 GTCGGAGTGG CCGTGGTCAA CTACAAGATG CCGCGCCTGC ACACCGCGGC GGAGGTCCTG
121 GACAACGCCC GGAAGATCGC CGACATGATC GTCGGCATGA AGCAGGGCCT GCCCGGCATG
181 GACCTGGTGG TGTTCCCGGA GTACAGCCTG CAGGGCATCA TGTACGATCC GGCGGAGATG
241 ATGGAAACCG CGGTGGCGAT CCCCGGCGAG GAAACCGAGA TATTCTCCCG CGCCTGCCGC
301 AAGGCCAACG TCTGGGGCGT ATTCTCCCTC ACCGGCGAAC GGCACGAGGA GCATCCGCGC
361 AAGGCGCCGT ACAACACCCT GGTGCTGATC GACAACAACG GCGAGATCGT CCAGAAGTAC
421 CGCAAGATCA TTCCCTGGTG CCCCATCGAG GGCTGGTATC CCGGTGGCCA GACCTACGTC
481 AGCGAAGGGC CGAAGGGCAT GAAGATCAGC CTGATCATCT GCGACGACCC CAATTACCCG
541 GAGATCTGGC GCGACTGCGC GATGAAGGGC GCCGAGCTGA TCGTGCGCTG CCAGGGCTAC
601 ATGTACCCGG CCAAGGACCA GCAGGTGATG ATGGCCAAGG CCATGGCCTG GGCCAACAAC
661 TGCTATGTGG CGGTGGCCAA CGCGGCCGGC TTCGACGGTG TCTATTCCTA CTTCGGCCAC
721 TCGGCGATCA TCGGCTTCGA CGGCCGTACC CTCGGTGAGT GCGGCGAGGA GGAAATGGGT
781 ATCCAGTACG CCCAGCTGTC CCTTTCGCAG ATCCGCGATG CGCGCGCCAA CGATCAGTCG
841 CAGAACCACC TGTTCAAGAT CCTCCACCGC GGCTACAGCG GCTTGCAGGC GTCCGGCGAC
901 GGCGACCGGG GCCTGGCGGA GTGTCCGTTC GAGTTCTACC GCACCTGGGT CACCGACGCC
961 GAGAAGGCGC GCGACAATGT CGAGCGACTG ACCCGCTCGA CCACCGGCGT GGCGCAATGC
1021 CCGGTCGGCC GGCTGCCCTA TGAGGGACTG GAGAAGGAAG CCTGA
//
LOCUS X13776 2167 bp DNA linear BCT 14-NOV-2006
DEFINITION Pseudomonas aeruginosa amiC and amiR gene for aliphatic amidase
regulation.
ACCESSION X13776 M43175
VERSION X13776.1 GI:45269
KEYWORDS aliphatic amidase regulator; amiC gene; amiR gene.
SOURCE Pseudomonas aeruginosa
ORGANISM Pseudomonas aeruginosa
Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
Pseudomonadaceae; Pseudomonas.
REFERENCE 1 (bases 1167 to 2167)
AUTHORS Lowe,N., Rice,P.M. and Drew,R.E.
TITLE Nucleotide sequence of the aliphatic amidase regulator gene (amiR)
of Pseudomonas aeruginosa
JOURNAL FEBS Lett. 246 (1-2), 39-43 (1989)
PUBMED 2495988
REFERENCE 2 (bases 1 to 1292)
AUTHORS Wilson,S. and Drew,R.
TITLE Cloning and DNA sequence of amiC, a new gene regulating expression
of the Pseudomonas aeruginosa aliphatic amidase, and purification
of the amiC product
JOURNAL J. Bacteriol. 173 (16), 4914-4921 (1991)
PUBMED 1907262
REFERENCE 3 (bases 1167 to 2167)
AUTHORS Rice,P.M.
TITLE Direct Submission
JOURNAL Submitted (16-DEC-1988) Rice P.M., EMBL, Postfach 10-2209,
Meyerhofstrasse 1, 6900 Heidelberg, FRG
REFERENCE 4 (bases 1 to 2167)
AUTHORS Rice,P.M.
TITLE Direct Submission
JOURNAL Submitted (04-SEP-1991) Rice P.M., EMBL, Postfach 10-2209,
Meyerhofstrasse 1, 6900 Heidelberg, FRG
COMMENT On Jul 25, 2002 this sequence version replaced gi:151357.
Data kindly reviewed (04-SEP-1991) by Rice P. M.
FEATURES Location/Qualifiers
source 1. .2167
/organism="Pseudomonas aeruginosa"
/mol_type="genomic DNA"
/strain="PAC"
/isolate="PAC 1"
/db_xref="taxon:287"
/map="38 min"
misc_feature 1
/note="last base of an XhoI site"
promoter 8. .24
/note="proposed rpoN-dependent promoter"
promoter 65. .81
/note="proposed rpoN-dependent promoter"
RBS 121. .126
/note="proposed Shine-Dalgarno sequence"
gene 135. .1292
/gene="amiC"
CDS 135. .1292
/gene="amiC"
/note="negative regulator of amiR"
/codon_start=1
/transl_table=11
/protein_id="CAA32024.1"
/db_xref="GI:45271"
/db_xref="GOA:P27017"
/db_xref="PDB:1PEA"
/db_xref="PDB:1QNL"
/db_xref="PDB:1QO0"
/db_xref="UniProtKB/Swiss-Prot:P27017"
/translation="MGSHQERPLIGLLFSETGVTADIERSHAYGALLAVEQLNREGGV
GGRPIETLSQDPGGDPDRYRLCAEDFIRNRGVRFLVGCYMSHTRKAVMPVVERADALL
CYPTPYEGFEYSPNIVYGGPAPNQNSAPLAAYLIRHYGERVVFIGSDYIYPRESNHVM
RHLYRQHGGTVLEEIYIPLYPSDDDLQRAVERIYQARADVVFSTVVGTGTAELYRAIA
RRYGDGRRPPIASLTTSEAEVAKMESDVAEGQVVVAPYFSSIDTPASRAFVQACHGFF
PENATITAWAEAAYWQTLLLGRAAQAAGNWRVEDVQRHLYDIDIDAPQGPVRVERQNN
HSRLSSRIAEIDARGVFQVRWQSPEPIRPDPYVVVHNLDDWSASMGGGPLP"
misc_feature 648. .653
/gene="amiC"
/note="end of 658bp XhoI fragment, deletion in pSW3 causes
constitutive expression of amiE"
variation 912. .1167
/gene="amiC"
/note="ClaI fragment deleted in pSW36, constitutive
phenotype"
/replace=""
conflict 1281
/gene="amiC"
/citation=[2]
/replace="g"
gene 1289. .1879
/gene="amiR"
CDS 1289. .1879
/gene="amiR"
/note="aliphatic amidase regulator, positive regulator of
amiE"
/codon_start=1
/transl_table=11
/protein_id="CAA32023.1"
/db_xref="GI:45270"
/db_xref="GOA:P10932"
/db_xref="PDB:1QO0"
/db_xref="UniProtKB/Swiss-Prot:P10932"
/translation="MSANSLLGSLRELQVLVLNPPGEVSDALVLQLIRIGCSVRQCWP
PPEAFDVPVDVVFTSIFQNGHHDEIAALLAAGTPRTTLVALVEYESPAVLSQIIELEC
HGVITQPLDAHRVLPVLVSARRISEEMAKLKQKTEQLQDRIAGQARINQAKVLLMQRH
GWDEREAHQHLSREAMKRREPILKIAQELLGNEPSA"
ORIGIN
1 GGTACCGCTG GCCGAGCATC TGCTCGATCA CCACCAGCCG GGCGACGGGA ACTGCACGAT
61 CTACCTGGCG AGCCTGGAGC ACGAGCGGGT TCGCTTCGTA CGGCGCTGAG CGACAGTCAC
121 AGGAGAGGAA ACGGATGGGA TCGCACCAGG AGCGGCCGCT GATCGGCCTG CTGTTCTCCG
181 AAACCGGCGT CACCGCCGAT ATCGAGCGCT CGCACGCGTA TGGCGCATTG CTCGCGGTCG
241 AGCAACTGAA CCGCGAGGGC GGCGTCGGCG GTCGCCCGAT CGAAACGCTG TCCCAGGACC
301 CCGGCGGCGA CCCGGACCGC TATCGGCTGT GCGCCGAGGA CTTCATTCGC AACCGGGGGG
361 TACGGTTCCT CGTGGGCTGC TACATGTCGC ACACGCGCAA GGCGGTGATG CCGGTGGTCG
421 AGCGCGCCGA CGCGCTGCTC TGCTACCCGA CCCCCTACGA GGGCTTCGAG TATTCGCCGA
481 ACATCGTCTA CGGCGGTCCG GCGCCGAACC AGAACAGTGC GCCGCTGGCG GCGTACCTGA
541 TTCGCCACTA CGGCGAGCGG GTGGTGTTCA TCGGCTCGGA CTACATCTAT CCGCGGGAAA
601 GCAACCATGT GATGCGCCAC CTGTATCGCC AGCACGGCGG CACGGTGCTC GAGGAAATCT
661 ACATTCCGCT GTATCCCTCC GACGACGACT TGCAGCGCGC CGTCGAGCGC ATCTACCAGG
721 CGCGCGCCGA CGTGGTCTTC TCCACCGTGG TGGGCACCGG CACCGCCGAG CTGTATCGCG
781 CCATCGCCCG TCGCTACGGC GACGGCAGGC GGCCGCCGAT CGCCAGCCTG ACCACCAGCG
841 AGGCGGAGGT GGCGAAGATG GAGAGTGACG TGGCAGAGGG GCAGGTGGTG GTCGCGCCTT
901 ACTTCTCCAG CATCGATACG CCCGCCAGCC GGGCCTTCGT CCAGGCCTGC CATGGTTTCT
961 TCCCGGAGAA CGCGACCATC ACCGCCTGGG CCGAGGCGGC CTACTGGCAG ACCTTGTTGC
1021 TCGGCCGCGC CGCGCAGGCC GCAGGCAACT GGCGGGTGGA AGACGTGCAG CGGCACCTGT
1081 ACGACATCGA CATCGACGCG CCACAGGGGC CGGTCCGGGT GGAGCGCCAG AACAACCACA
1141 GCCGCCTGTC TTCGCGCATC GCGGAAATCG ATGCGCGCGG CGTGTTCCAG GTCCGCTGGC
1201 AGTCGCCCGA ACCGATTCGC CCCGACCCTT ATGTCGTCGT GCATAACCTC GACGACTGGT
1261 CCGCCAGCAT GGGCGGGGGA CCGCTCCCAT GAGCGCCAAC TCGCTGCTCG GCAGCCTGCG
1321 CGAGTTGCAG GTGCTGGTCC TCAACCCGCC GGGGGAGGTC AGCGACGCCC TGGTCTTGCA
1381 GCTGATCCGC ATCGGTTGTT CGGTGCGCCA GTGCTGGCCG CCGCCGGAAG CCTTCGACGT
1441 GCCGGTGGAC GTGGTCTTCA CCAGCATTTT CCAGAATGGC CACCACGACG AGATCGCTGC
1501 GCTGCTCGCC GCCGGGACTC CGCGCACTAC CCTGGTGGCG CTGGTGGAGT ACGAAAGCCC
1561 CGCGGTGCTC TCGCAGATCA TCGAGCTGGA GTGCCACGGC GTGATCACCC AGCCGCTCGA
1621 TGCCCACCGG GTGCTGCCTG TGCTGGTATC GGCGCGGCGC ATCAGCGAGG AAATGGCGAA
1681 GCTGAAGCAG AAGACCGAGC AGCTCCAGGA CCGCATCGCC GGCCAGGCCC GGATCAACCA
1741 GGCCAAGGTG TTGCTGATGC AGCGCCATGG CTGGGACGAG CGCGAGGCGC ACCAGCACCT
1801 GTCGCGGGAA GCGATGAAGC GGCGCGAGCC GATCCTGAAG ATCGCTCAGG AGTTGCTGGG
1861 AAACGAGCCG TCCGCCTGAG CGATCCGGGC CGACCAGAAC AATAACAAGA GGGGTATCGT
1921 CATCATGCTG GGACTGGTTC TGCTGTACGT TGGCGCGGTG CTGTTTCTCA ATGCCGTCTG
1981 GTTGCTGGGC AAGATCAGCG GTCGGGAGGT GGCGGTGATC AACTTCCTGG TCGGCGTGCT
2041 GAGCGCCTGC GTCGCGTTCT ACCTGATCTT TTCCGCAGCA GCCGGGCAGG GCTCGCTGAA
2101 GGCCGGAGCG CTGACCCTGC TATTCGCTTT TACCTATCTG TGGGTGGCCG CCAACCAGTT
2161 CCTCGAG
//
LOCUS X77161 1130 bp DNA linear BCT 31-JUL-2003
DEFINITION Pseudomonas aeruginosa (PAC1) amiS gene.
ACCESSION X77161
VERSION X77161.1 GI:473110
KEYWORDS amiS gene.
SOURCE Pseudomonas aeruginosa
ORGANISM Pseudomonas aeruginosa
Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
Pseudomonadaceae; Pseudomonas.
REFERENCE 1 (bases 1 to 1130)
AUTHORS Wilson,S.A., Williams,R.J., Pearl,L.H. and Drew,R.E.
TITLE Identification of two new genes in the Pseudomonas aeruginosa
amidase operon, encoding an ATPase (AmiB) and a putative integral
membrane protein (AmiS)
JOURNAL J. Biol. Chem. 270 (32), 18818-18824 (1995)
PUBMED 7642533
REFERENCE 2
AUTHORS Wilson,S.A., Wachira,S.M., Drew,R.E., Jones,D. and Pearl,L.H.
TITLE Identification of a putative ABC transporter in the Pseudomonas
aeruginosa amidase operon
JOURNAL Unpublished
REFERENCE 3 (bases 1 to 1130)
AUTHORS Wilson,S.A.
TITLE Direct Submission
JOURNAL Submitted (17-JAN-1994) S.A. Wilson, University College London,
Dept of Biochemistry, Gower Street, London WC1E 6BT, UK
FEATURES Location/Qualifiers
source 1. .1130
/organism="Pseudomonas aeruginosa"
/mol_type="genomic DNA"
/strain="PAC1"
/db_xref="taxon:287"
/clone="plasmid pAS20"
gene 61. .576
/gene="amiS"
CDS 61. .576
/gene="amiS"
/codon_start=1
/transl_table=11
/protein_id="CAA54406.1"
/db_xref="GI:473111"
/db_xref="GOA:Q51417"
/db_xref="UniProtKB/Swiss-Prot:Q51417"
/translation="MLGLVLLYVGAVLFLNAVWLLGKISGREVAVINFLVGVLSACVA
FYLIFSAAAGQGSLKAGALTLLFAFTYLWVAANQFLEVDGKGLGWFCLFVSLTACTVA
IESFAGASGPFGLWNAVNWTVWALLWFCFFLLLGLSRGIQKPVAYLTLASAIFTAWLP
GLLLLGQVLKA"
ORIGIN
1 GAGCCGTCCG CCTGAGCGAT CCGGGCCGAC CAGAACAATA ACAAGAGGGG TATCGTCATC
61 ATGCTGGGAC TGGTTCTGCT GTACGTTGGC GCGGTGCTGT TTCTCAATGC CGTCTGGTTG
121 CTGGGCAAGA TCAGCGGTCG GGAGGTGGCG GTGATCAACT TCCTGGTCGG CGTGCTGAGC
181 GCCTGCGTCG CGTTCTACCT GATCTTTTCC GCAGCAGCCG GGCAGGGCTC GCTGAAGGCC
241 GGAGCGCTGA CCCTGCTATT CGCTTTTACC TATCTGTGGG TGGCCGCCAA CCAGTTCCTC
301 GAGGTGGACG GCAAGGGCCT CGGCTGGTTC TGCCTGTTCG TCAGCCTCAC CGCCTGCACC
361 GTGGCGATCG AGTCGTTCGC CGGCGCCAGT GGTCCGTTCG GCCTGTGGAA CGCGGTCAAC
421 TGGACAGTCT GGGCGTTGCT CTGGTTCTGT TTCTTCCTGC TGCTGGGGCT GTCCCGCGGC
481 ATCCAGAAGC CGGTGGCCTA CCTGACCCTG GCCAGCGCCA TATTCACCGC CTGGTTGCCC
541 GGCCTGCTGC TGCTCGGACA GGTGCTCAAG GCATAGCAGG AAGTCGGAAA GGGATGACGG
601 CTTGCCGCCA TCCCGTCCCT TCCGAACGCC TAGCCGAGCG GCCAGTTGAT CACCACGACG
661 GCGTCGTTGT AGTCGTTGTC GGTGCCGTCT TCAGAGCCGA CCAGGGCGAA GTTCAGCTCG
721 TTGGTCAGGA TTACCTGTGC CGAGACCAGA TCCGAGGGGC GGCCGTTGAC GCTGACCTGG
781 ACCTGTACCT TGCCACTGCT GCCGGAGTTG AGCACCTGGG TGCCGATGAC GGCGTTATTG
841 GTGCTTTGCC CGCTGAAGGT CGCGGCCGTG CTCGTTGTTG ACCAGCACGT TCACCGTCTG
901 GGTTCCGGAC GAGTTGGCGA AGGCGGTGAC GCCGGAACCT GGTTGTTGGC GGGAAGGGTG
961 AACACTCCTT GTGGTTGCCA TGGTGGTATC TCCACTGAAT ACCTGGCCCC TTCCTTTTCA
1021 GGCAGCCGTC TGGCGCGCGG TATGGCGTGT CGGGAGAAAT CCGCAGTCCT TGGCGGCAGG
1081 CGATGCGCAG GCAGGAAGGA CGCATCGTTC AGCCAATCTA CGCCGTCGAC
//
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