/usr/lib/python2.7/dist-packages/cogent/format/rna_struct.py is in python-cogent 1.9-9.
This file is owned by root:root, with mode 0o644.
The actual contents of the file can be viewed below.
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"""
Writer functions for RNA 2D structures.
NOTE: Still in beta testing.
"""
from matplotlib import use
use('Agg') #suppress graphical rendering
from cogent.app.vienna_package import plot_from_seq_and_struct
from cogent.parse.rna_plot import RnaPlotParser
from cogent.parse.record_finder import LabeledRecordFinder
from matplotlib.patches import Circle, Rectangle, Polygon
from matplotlib.text import Text
from pylab import gcf,gca, draw, savefig, clf
from math import ceil
__author__ = "Jeremy Widmann"
__copyright__ = "Copyright 2007-2016, The Cogent Project"
__credits__ = ["Jeremy Widmann"]
__license__ = "GPL"
__version__ = "1.9"
__maintainer__ = "Jeremy Widmann"
__email__ = "jeremy.widmann@colorado.edu"
__status__ = "Development"
################################################################################
##### Code for postscript RNA 2d Struct ########################################
################################################################################
COLOR_FUNCTION_STRING = \
"""/Colormark { % i color Colormark draw circle around base i
% setcolor
setrgbcolor
newpath 1 sub coor exch get aload pop
fsize 2 div 0 360 arc fill stroke
} bind def
/Backcolor { % i color Backcolor draw circle around base i
% setcolor
setrgbcolor
newpath 1 sub coor exch get aload pop
fsize 2.6 div 0 360 arc fill stroke
} bind def
/Backgroundbases {
indices {
WHITE Backcolor
} forall
} bind def"""
#Draw structure outline, base pairs, and base background.
INIT_STRUCT_COMMAND = \
"""drawoutline
drawpairs
Backgroundbases
"""
def get_indices_string(sequence):
"""Returns postscript index definition string.
- sequence: string, list, etc representing the sequence of the RNA.
Calling len(sequence) must give the length of the sequence.
"""
index_string = '/indices [%s] def'%(' '.join(map(str,\
[i+1 for i in range(len(sequence))])))
return index_string
def get_color_map_string(color_map):
"""Returns postscript color map string given color map.
- color_map: mapping from name to RGB color. {name:[R,G,B]}
- Name must be a string with no spaces.
- [R, G, B] must be a list of fractional RGB values from 0 to 1.
eg {"red": [1.0, 0.0, 0.0]
"""
color_list = []
for name, color in color_map.items():
r,g,b = color
color_list.append('/%s { %s %s %s } def'%(name,r,g,b))
return '\n'.join(color_list)
def get_color_commands(indices, colors):
"""Returns postscript coloring string given indices and colors.
- indices: base 1 index of color. NOT BASE 0.
- colors: color name corresponding to color name used to generate
color_map.
- indices and colors must be lists of the same length.
"""
color_commands = []
for index, color in zip(indices,colors):
color_commands.append('%s %s Colormark'%(str(index),color))
return ' '.join(color_commands)
def get_circle_commands(indices, color='seqcolor'):
"""Returns postscript circling string given indices.
- indices: base 1 index of color. NOT BASE 0.
- color: color name of circle.
"""
circle_commands = []
if indices:
circle_commands.append(color)
for index in indices:
circle_commands.append(str(index)+' cmark')
return ' '.join(circle_commands)
def get_rnaplot_postscript(sequence, struct):
"""Returns postscript string for seq and struct.
"""
#Params for RNAplot
params = {'-t':'0',\
'--pre':'%PreTextHere'}
#Get the postscript list
ps_list = plot_from_seq_and_struct(sequence,\
struct,params=params).split('\n')
#parse it into prefix and suffix lists
pre_finder = LabeledRecordFinder(\
is_label_line=lambda x: x.startswith('%PreTextHere'))
prefix,suffix = list(pre_finder(ps_list))
#Remove drawoutline and drawpairs commands form suffix
new_suffix = []
for s in suffix:
if not (s.startswith('drawpairs') or s.startswith('drawoutline')):
new_suffix.append(s)
return '\n'.join(prefix), '\n'.join(new_suffix)
def color_on_structure(sequence,struct,color_map,indices=None,colors=None,\
circle_indices=None):
"""Returns a postscript string colored at indices.
"""
if indices is None:
indices = []
if colors is None:
colors = []
if len(indices) != len(colors):
raise ValueError, 'indices and colors must be equal sized lists'
#Get indices string
indices_string = get_indices_string(str(sequence))
#Get color map string
color_map_string = get_color_map_string(color_map)
#Get color commands
color_commands = get_color_commands(indices,colors)
#Get circle commands
circle_commands = get_circle_commands(circle_indices)
#get RNAplot postscript
prefix, suffix = get_rnaplot_postscript(sequence, struct)
return '\n'.join([prefix,COLOR_FUNCTION_STRING,\
indices_string,INIT_STRUCT_COMMAND,\
color_map_string,color_commands,circle_commands,suffix])
################################################################################
##### END Code for postscript RNA 2d Struct ####################################
################################################################################
################################################################################
##### Code for matplotlib RNA 2d Struct ########################################
################################################################################
def scale_coords(coords):
"""Returns coordinate list scaled to matplotlib coordintates.
- coords: list of lists, of x,y coordinates:
[[x1,y1],[x2,y2],[x3,y3],...]
"""
new_coords = []
#get min and max x coordinates
max_x = max([c[0] for c in coords])
min_x = min([c[0] for c in coords])
#get min and max y coordinates
max_y = max([c[1] for c in coords])
min_y = min([c[1] for c in coords])
#Get scaled max values for x and y
scaled_max_x = max_x - min_x
scaled_max_y = max_y - min_y
#max scale value
max_scale = max(scaled_max_x, scaled_max_y)
scale_x = min_x
scale_y = min_y
for x,y in coords:
new_coords.append([(x-scale_x)/max_scale,\
(y-scale_y)/max_scale])
return new_coords
def set_axis_limits(axis, all_coords):
"""Sets axis limits based on all coordinates preventing clipping.
axis: from calling gca()
all_coords: all coordinates for shapes and labels.
"""
all_x = [x[0] for x in all_coords]
all_y = [x[1] for x in all_coords]
offset = abs(all_coords[0][0]-all_coords[1][0]) + \
abs(all_coords[0][1]-all_coords[1][1])
min_x = min(all_x)
max_x = max(all_x)
min_y = min(all_y)
max_y = max(all_y)
axis.set_xlim((-offset,1.0+offset))
axis.set_ylim((-offset,1.0+offset))
def make_circles(coords,facecolors,edgecolors,radius=0.02,alpha=1.0,fill=False):
"""Returns list of Circle objects, given list of coordinates.
- coords: list of [x,y] coordinates, already scaled to matplotlib axes.
- facecolor: color of circle face.
- edgecolor: color of circle edge.
- radius: radius of circle.
"""
recenter_divide = radius*100.
recenter = radius/recenter_divide
circles = []
for coord, facecolor,edgecolor in zip(coords,facecolors,edgecolors):
x_coord,y_coord = coord
curr_circle = Circle([x_coord+recenter,y_coord+recenter],\
radius=radius,facecolor=facecolor,edgecolor=edgecolor,alpha=alpha,\
fill=fill)
circles.append(curr_circle)
return circles
def make_boxes(coords, facecolor='white', edgecolor='black', edge_size=0.03,\
alpha=1.0,fill=False):
"""Returns list of Rectangle objects, given list of coordinates.
- coords: list of [x,y] coordinates, already scaled to matplotlib axes.
- facecolor: color of box face.
- edgecolor: color of box edge.
- edge_size: length of box edges.
"""
boxes = []
recenter_divide = edge_size*200.
recenter = edge_size/recenter_divide
for x_coord,y_coord in coords:
curr_box = Rectangle([x_coord-recenter,y_coord-recenter],\
edge_size,edge_size,\
facecolor=facecolor,edgecolor=edgecolor,alpha=alpha,fill=fill)
boxes.append(curr_box)
return boxes
def make_letters(coords,letters,color='black'):
"""Returns list of Text objects, given list of coordinates and letteres.
- coords: list of [x,y] coordinates, already scaled to matplotlib axes.
- letters: list of letters to be drawn at given coordinates. Must be
same size list as coords.
- color: color of the letters.
"""
letter_list = []
for coord, letter in zip(coords, letters):
x_coord, y_coord = coord
curr_letter = Text(x_coord, y_coord, letter)
letter_list.append(curr_letter)
return letter_list
def make_pairs(coords,pair_indices,offset=.01):
"""Returns list of Polygon objects, given a list of coordinates and indices.
- coords: list of [x,y] coordinates, already scaled to matplotlib axes.
- pair_indices: indices in the coordinate list that are paired.
"""
pairs = []
for first, second in pair_indices:
fx, fy = coords[first]
sx, sy = coords[second]
pairs.append(Polygon([[fx+offset,fy+offset],[sx+offset,sy+offset]],\
alpha=0.2,linewidth=2))
return pairs
def make_outline(coords,offset=.01):
"""Returns Polygon object given coords.
"""
outline_coords = [[x+offset,y+offset] for x,y in coords]
outline = Polygon(outline_coords,alpha=0.2,linewidth=2,facecolor='white')
finish = Polygon([outline_coords[0],outline_coords[-1]],\
alpha=1,linewidth=3,edgecolor='white',facecolor='white')
return [outline,finish]
def make_labels(coords):
"""Returns Text objects with 5' and 3' labels.
"""
#get five prime coordinates
fp_x = coords[0][0] - (coords[1][0] - coords[0][0])
fp_y = coords[0][1] - (coords[1][1] - coords[0][1])
fp_label = Text(fp_x,fp_y,"5'")
#get three prime coordinates
tp_x = coords[-1][0] - (coords[-2][0] - coords[-1][0])
tp_y = coords[-1][1] - (coords[-2][1] - coords[-1][1])
tp_label = Text(tp_x,tp_y,"3'")
return [fp_label,tp_label], [[fp_x,fp_y],[tp_x,tp_y]]
def draw_structure(sequence,struct,indices=None,colors=None,\
circle_indices=None, square_indices=None,radial=True):
"""Returns a postscript string colored at indices.
sequence: string of sequence characters.
struct: string of ViennaStructure for sequence. Must be valid structure
same length as sequence.
indices: list of indices in sequence that will be colored as a solid
circle.
colors: list of colors, same length as list of indices.
circle_indices: list of indices in sequence to draw an empty circle
around.
square_indices: list of indices in sequence to draw an empty square
around.
radial: draw structue in radial format (default=True).
"""
seq_len_scale = int(len(sequence)/50.)
if seq_len_scale < 1:
circle_scale_size = 0.
square_scale_size = 0.
else:
#Get circle radius. Proportional to sequence length
circle_scale_size = (.02/seq_len_scale)/4.0
#Get edge size. Proportional to sequence length
square_scale_size = (.03/seq_len_scale)/4.0
circle_radius = .02 - circle_scale_size
square_edge_size = .03 - square_scale_size
if indices is None:
indices = []
if colors is None:
colors = []
if circle_indices is None:
circle_indices = []
if square_indices is None:
square_indices = []
if len(indices) != len(colors):
raise ValueError, 'indices and colors must be equal sized lists'
if radial:
params = {'-t':'0'}
else:
params = {'-t':'1'}
#Get the postscript list
ps_list = plot_from_seq_and_struct(sequence,\
struct,params=params).split('\n')
#Parse out seq, coords, and pairs
seq, coords, pair_list = RnaPlotParser(ps_list)
coords = scale_coords(coords)
#get letters
letters = make_letters(coords, list(seq))
#get pairs
pairs = make_pairs(coords, pair_list)
#get outline
outline = make_outline(coords)
#get labels
labels,label_coords = make_labels(coords)
#get plain circle coords
circle_coords = [coords[i] for i in circle_indices]
circle_faces = ['white']*len(circle_coords)
circle_edges = ['black']*len(circle_coords)
plain_circles = make_circles(circle_coords,circle_faces, circle_edges,\
radius=circle_radius)
#get motif circles
motif_coords = [coords[i] for i in indices]
motif_circles = make_circles(motif_coords,colors,colors,fill=True,\
radius=circle_radius)
#Get square coords
square_coords = [coords[i] for i in square_indices]
plain_squares = make_boxes(square_coords,edge_size=square_edge_size)
axis = gca()
axis.set_axis_off()
all_coords = coords + label_coords
set_axis_limits(axis, all_coords)
for l in [letters, pairs, outline, motif_circles, plain_circles, \
plain_squares,labels]:
for shape in l:
axis.add_artist(shape)
fig = gcf()
largest = max([fig.get_figheight(), fig.get_figwidth()])
fig.set_figheight(largest)
fig.set_figwidth(largest)
def save_structure(sequence,struct,out_filename,indices=None,colors=None,\
circle_indices=None, square_indices=None,radial=True,format='png',\
dpi=75):
"""Saves figure of 2D structure generated by draw_structure().
sequence: string of sequence characters.
struct: string of ViennaStructure for sequence. Must be valid structure
same length as sequence.
indices: list of indices in sequence that will be colored as a solid
circle.
colors: list of colors, same length as list of indices.
circle_indices: list of indices in sequence to draw an empty circle
around.
square_indices: list of indices in sequence to draw an empty square
around.
radial: draw structue in radial format (default=True).
format: format of structure figure (default=png). Must be a valid
matplotlib format.
dpi: resolution of figure (default=75).
"""
draw_structure(sequence,struct,indices=indices,colors=colors,\
circle_indices=circle_indices,\
square_indices=square_indices,radial=radial)
savefig(out_filename,format=format,dpi=dpi)
clf()
################################################################################
##### End Code for matplotlib RNA 2d Struct ####################################
################################################################################
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