/usr/share/perl5/Bio/Roary/Output/GroupsMultifastaNucleotide.pm is in roary 3.8.0+dfsg-1.
This file is owned by root:root, with mode 0o644.
The actual contents of the file can be viewed below.
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96 97 98 99 100 101 102 103 104 105 106 107 108 109 110 111 112 113 114 115 116 117 118 119 120 121 122 123 124 125 126 127 128 129 130 131 132 133 134 135 136 137 138 139 140 141 142 143 144 145 146 147 148 149 150 151 152 153 154 155 156 157 158 159 160 161 162 163 164 165 166 167 168 169 170 171 172 173 174 175 176 177 178 179 180 181 182 183 184 185 186 187 188 189 190 191 192 193 194 195 196 197 198 199 200 201 202 203 204 205 206 207 208 209 210 211 212 213 214 215 216 217 218 219 220 221 222 223 224 225 226 227 228 229 230 231 232 | package Bio::Roary::Output::GroupsMultifastaNucleotide;
$Bio::Roary::Output::GroupsMultifastaNucleotide::VERSION = '3.8.0';
# ABSTRACT: Take in a GFF files and a groups file and output one multifasta file per group with nucleotide sequences.
use Moose;
use Bio::SeqIO;
use File::Path qw(make_path);
use File::Basename;
use File::Copy;
use File::Temp qw/ tempfile /;
use Bio::Roary::Exceptions;
use Bio::Roary::AnalyseGroups;
use Bio::Tools::GFF;
with 'Bio::Roary::BedFromGFFRole';
has 'gff_file' => ( is => 'ro', isa => 'Str', required => 1 );
has 'group_names' => ( is => 'ro', isa => 'ArrayRef', required => 0 );
has 'output_directory' => ( is => 'ro', isa => 'Str', required => 1 );
has 'pan_reference_groups_seen' => ( is => 'rw', isa => 'HashRef', required => 1 );
has 'number_of_gff_files' => ( is => 'ro', isa => 'Int', required => 1 );
has 'pan_reference_filename' => ( is => 'ro', isa => 'Str',default => 'pan_genome_reference.fa' );
has 'dont_delete_files' => ( is => 'ro', isa => 'Bool',default => 0 );
has 'core_definition' => ( is => 'ro', isa => 'Num', default => 1.0 );
has 'annotate_groups' => ( is => 'ro', isa => 'Bio::Roary::AnnotateGroups', required => 1 );
has 'output_multifasta_files' => ( is => 'ro', isa => 'Bool', default => 0 );
has 'fasta_file' => ( is => 'ro', isa => 'Str', lazy => 1, builder => '_build_fasta_file' );
has '_input_seqio' => ( is => 'ro', isa => 'Bio::SeqIO', lazy => 1, builder => '_build__input_seqio' );
has 'output_filename' => ( is => 'ro', isa => 'Str', lazy => 1, builder => '_build_output_filename' );
sub _build_output_filename
{
my ($self) = @_;
my ( $filename, $directories, $suffix ) = fileparse($self->gff_file);
return join('/',($self->output_directory, $filename.'.tmp_nuc_sequences.fa' ));
}
sub _build__input_seqio {
my ($self) = @_;
return Bio::SeqIO->new( -file => $self->fasta_file, -format => 'Fasta' );
}
sub _bed_output_filename {
my ($self) = @_;
return join( '.', ( $self->output_filename, 'intermediate.bed' ) );
}
sub populate_files {
my ($self) = @_;
while ( my $input_seq = $self->_input_seqio->next_seq() )
{
if ( $self->annotate_groups->_ids_to_groups->{$input_seq->display_id} )
{
my $current_group = $self->annotate_groups->_ids_to_groups->{$input_seq->display_id};
my $gene_name = $self->annotate_groups->_groups_to_consensus_gene_names->{$current_group};
if(! defined($self->pan_reference_groups_seen->{$current_group}))
{
my $pan_output_seq = $self->_pan_genome_reference_io_obj($current_group);
$pan_output_seq->write_seq(Bio::Seq->new( -display_id => $input_seq->display_id, -desc => ($gene_name ? $gene_name : $current_group), -seq => $input_seq->seq ) );
$self->pan_reference_groups_seen->{$current_group} = 1;
}
my $number_of_genes = @{$self->annotate_groups->_groups_to_id_names->{$current_group}};
# Theres no need to align noncore files
next if($self->dont_delete_files == 0 && $number_of_genes < ($self->core_definition * $self->number_of_gff_files ));
my $output_seq = $self->_group_seq_io_obj($current_group,$number_of_genes);
$output_seq->write_seq($input_seq);
}
}
unlink($self->fasta_file);
1;
}
sub _group_file_name
{
my ($self,$group_name,$num_group_genes) = @_;
my $annotated_group_name = $self->annotate_groups->_groups_to_consensus_gene_names->{$group_name};
$annotated_group_name =~ s!\W!_!gi;
my $filename = $annotated_group_name.'.fa';
my $group_file_name = join('/',($self->output_directory, $filename ));
return $group_file_name;
}
sub _pan_genome_reference_io_obj
{
my ($self) = @_;
return Bio::SeqIO->new( -file => ">>".$self->pan_reference_filename, -format => 'Fasta' );
}
sub _group_seq_io_obj
{
my ($self,$group_name,$num_group_genes) = @_;
my $filename = $self->_group_file_name($group_name,$num_group_genes);
return Bio::SeqIO->new( -file => ">>".$filename, -format => 'Fasta' );
}
sub _extracted_nucleotide_fasta_file_from_bed_filename {
my ($self) = @_;
return join( '.', ( $self->output_filename, 'intermediate.extracted.fa' ) );
}
sub _create_nucleotide_fasta_file_from_gff {
my ($self) = @_;
open(my $input_fh, $self->gff_file);
open(my $output_fh, '>', $self->_nucleotide_fasta_file_from_gff_filename);
my $at_sequence = 0;
while(<$input_fh>)
{
my $line = $_;
if($line =~/^>/)
{
$at_sequence = 1;
}
if($at_sequence == 1)
{
print {$output_fh} $line;
}
}
close($input_fh);
close($output_fh);
}
sub _nucleotide_fasta_file_from_gff_filename {
my ($self) = @_;
return join( '.', ( $self->output_filename, 'intermediate.fa' ) );
}
sub _extract_nucleotide_regions {
my ($self) = @_;
$self->_create_nucleotide_fasta_file_from_gff;
$self->_create_bed_file_from_gff;
my $cmd =
'bedtools getfasta -s -fi '
. $self->_nucleotide_fasta_file_from_gff_filename
. ' -bed '
. $self->_bed_output_filename . ' -fo '
. $self->_extracted_nucleotide_fasta_file_from_bed_filename
. ' -name > /dev/null 2>&1';
system($cmd);
unlink( $self->_nucleotide_fasta_file_from_gff_filename );
unlink( $self->_bed_output_filename );
unlink( $self->_nucleotide_fasta_file_from_gff_filename . '.fai' );
return $self->_extracted_nucleotide_fasta_file_from_bed_filename;
}
sub _cleanup_fasta {
my ($self,$infile) = @_;
my($fh, $outfile) = tempfile();
return unless ( -e $infile );
open( my $in, '<', $infile );
open( my $out, '>', $outfile );
while ( my $line = <$in> ) {
chomp $line;
$line =~ s/"//g if ( $line =~ /^>/ );
if($line =~ /^(>[^:]+)/)
{
$line = $1;
}
print $out "$line\n";
}
close $in;
close $out;
move( $outfile, $infile);
return $infile;
}
sub _build_fasta_file {
my ($self) = @_;
my $fasta_filename = $self->_extract_nucleotide_regions;
return $self->_cleanup_fasta($fasta_filename);
}
no Moose;
__PACKAGE__->meta->make_immutable;
1;
__END__
=pod
=encoding UTF-8
=head1 NAME
Bio::Roary::Output::GroupsMultifastaNucleotide - Take in a GFF files and a groups file and output one multifasta file per group with nucleotide sequences.
=head1 VERSION
version 3.8.0
=head1 SYNOPSIS
Take in a GFF files and a groups file and output one multifasta file per group with nucleotide sequences.
use Bio::Roary::Output::GroupsMultifastas;
my $obj = Bio::Roary::Output::GroupsMultifastasNucleotide->new(
group_names => ['aaa','bbb'],
);
$obj->populate_files();
=head1 AUTHOR
Andrew J. Page <ap13@sanger.ac.uk>
=head1 COPYRIGHT AND LICENSE
This software is Copyright (c) 2013 by Wellcome Trust Sanger Institute.
This is free software, licensed under:
The GNU General Public License, Version 3, June 2007
=cut
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