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<?xml version="1.0" encoding="ISO-8859-1"?>

<!-- XML Authors: Corinne Maufrais and Nicolas Joly,                             -->
<!-- 'Biological Software and Databases' Group, Institut Pasteur, Paris.         -->
<!-- Distributed under LGPLv2 License. Please refer to the COPYING.LIB document. -->

<program>
  <head>
    <name>getorf</name>
    <package>
      <name>EMBOSS</name>
      <version>6.3.1</version>
      <doc>
        <title>EMBOSS</title>
        <description>
          <text lang="en">European Molecular Biology Open Software Suite</text>
        </description>
        <authors>Rice,P. Longden,I. and Bleasby, A.</authors>
        <reference>EMBOSS: The European Molecular Biology Open Software Suite (2000)  Rice,P. Longden,I. and Bleasby, A. Trends in Genetics 16, (6) pp276--277</reference>
        <sourcelink>http://emboss.sourceforge.net/download</sourcelink>
        <homepagelink>http://emboss.sourceforge.net</homepagelink>
      </doc>
    </package>
    <doc>
      <title>getorf</title>
      <description>
        <text lang="en">Finds and extracts open reading frames (ORFs)</text>
      </description>
      <doclink>http://bioweb2.pasteur.fr/docs/EMBOSS/getorf.html</doclink>
      <doclink>http://emboss.sourceforge.net/docs/themes</doclink>
    </doc>
    <category>sequence:nucleic:gene_finding</category>
    <command>getorf</command>
  </head>

  <parameters>

    <paragraph>
      <name>e_input</name>
      <prompt lang="en">Input section</prompt>

      <parameters>

        <parameter issimple="1" ismandatory="1">
          <name>e_sequence</name>
          <prompt lang="en">sequence option</prompt>
          <type>
            <biotype>DNA</biotype>
            <datatype>
              <class>Sequence</class>
            </datatype>
            <dataFormat>EMBL</dataFormat>
            <dataFormat>FASTA</dataFormat>
            <dataFormat>GCG</dataFormat>
            <dataFormat>GENBANK</dataFormat>
            <dataFormat>NBRF</dataFormat>
            <dataFormat>CODATA</dataFormat>
            <dataFormat>RAW</dataFormat>
            <dataFormat>SWISSPROT</dataFormat>
            <dataFormat>GFF</dataFormat>
            <card>1,n</card>
          </type>
          <format>
            <code proglang="python">("", " -sequence=" + str(value))[value is not None]</code>
          </format>
          <argpos>1</argpos>
        </parameter>
      </parameters>
    </paragraph>

    <paragraph>
      <name>e_additional</name>
      <prompt lang="en">Additional section</prompt>

      <parameters>

        <parameter>
          <name>e_table</name>
          <prompt lang="en">Genetic codes</prompt>
          <type>
            <datatype>
              <class>Choice</class>
            </datatype>
          </type>
          <vdef>
            <value>0</value>
          </vdef>
          <vlist>
            <velem>
              <value>0</value>
              <label>Standard</label>
            </velem>
            <velem>
              <value>1</value>
              <label>Standard (with alternative initiation codons)</label>
            </velem>
            <velem>
              <value>2</value>
              <label>Vertebrate mitochondrial</label>
            </velem>
            <velem>
              <value>3</value>
              <label>Yeast mitochondrial</label>
            </velem>
            <velem>
              <value>4</value>
              <label>Mold, protozoan, coelenterate mitochondrial and  mycoplasma/spiroplasma</label>
            </velem>
            <velem>
              <value>5</value>
              <label>Invertebrate mitochondrial</label>
            </velem>
            <velem>
              <value>6</value>
              <label>Ciliate  macronuclear and dasycladacean</label>
            </velem>
            <velem>
              <value>9</value>
              <label>Echinoderm mitochondrial</label>
            </velem>
            <velem>
              <value>10</value>
              <label>Euplotid nuclear</label>
            </velem>
            <velem>
              <value>11</value>
              <label>Bacterial</label>
            </velem>
            <velem>
              <value>12</value>
              <label>Alternative yeast nuclear</label>
            </velem>
            <velem>
              <value>13</value>
              <label>Ascidian mitochondrial</label>
            </velem>
            <velem>
              <value>14</value>
              <label>Flatworm mitochondrial</label>
            </velem>
            <velem>
              <value>15</value>
              <label>Blepharisma macronuclear</label>
            </velem>
            <velem>
              <value>16</value>
              <label>Chlorophycean mitochondrial</label>
            </velem>
            <velem>
              <value>21</value>
              <label>Trematode mitochondrial</label>
            </velem>
            <velem>
              <value>22</value>
              <label>Scenedesmus obliquus</label>
            </velem>
            <velem>
              <value>23</value>
              <label>Thraustochytrium mitochondrial</label>
            </velem>
          </vlist>
          <format>
            <code proglang="python">("", " -table=" + str(value))[value is not None and value!=vdef]</code>
          </format>
          <argpos>2</argpos>
        </parameter>

        <parameter>
          <name>e_minsize</name>
          <prompt lang="en">Minimum nucleotide size of orf to report</prompt>
          <type>
            <datatype>
              <class>Integer</class>
            </datatype>
          </type>
          <vdef>
            <value>30</value>
          </vdef>
          <format>
            <code proglang="python">("", " -minsize=" + str(value))[value is not None and value!=vdef]</code>
          </format>
          <argpos>3</argpos>
        </parameter>

        <parameter>
          <name>e_maxsize</name>
          <prompt lang="en">Maximum nucleotide size of orf to report</prompt>
          <type>
            <datatype>
              <class>Integer</class>
            </datatype>
          </type>
          <vdef>
            <value>1000000</value>
          </vdef>
          <format>
            <code proglang="python">("", " -maxsize=" + str(value))[value is not None and value!=vdef]</code>
          </format>
          <argpos>4</argpos>
        </parameter>

        <parameter>
          <name>e_find</name>
          <prompt lang="en">Type of sequence to output</prompt>
          <type>
            <datatype>
              <class>Choice</class>
            </datatype>
          </type>
          <vdef>
            <value>0</value>
          </vdef>
          <vlist>
            <velem>
              <value>0</value>
              <label>Translation of regions between stop codons</label>
            </velem>
            <velem>
              <value>1</value>
              <label>Translation of regions between start and stop codons</label>
            </velem>
            <velem>
              <value>2</value>
              <label>Nucleic sequences between stop codons</label>
            </velem>
            <velem>
              <value>3</value>
              <label>Nucleic sequences  between start and stop codons</label>
            </velem>
            <velem>
              <value>4</value>
              <label>Nucleotides flanking start  codons</label>
            </velem>
            <velem>
              <value>5</value>
              <label>Nucleotides flanking initial stop codons</label>
            </velem>
            <velem>
              <value>6</value>
              <label>Nucleotides flanking ending stop codons</label>
            </velem>
          </vlist>
          <format>
            <code proglang="python">("", " -find=" + str(value))[value is not None and value!=vdef]</code>
          </format>
          <argpos>5</argpos>
          <comment>
            <text lang="en">This is a small menu of possible output options. The first four options are to select either the protein translation or the  original nucleic acid sequence of the open reading frame. There  are two possible definitions of an open reading frame: it can  either be a region that is free of STOP codons or a region that  begins with a START codon and ends with a STOP codon. The last  three options are probably only of interest to people who wish to  investigate the statistical properties of the regions around  potential START or STOP codons. The last option assumes that ORF  lengths are calculated between two STOP codons.</text>
          </comment>
        </parameter>
      </parameters>
    </paragraph>

    <paragraph>
      <name>e_advanced</name>
      <prompt lang="en">Advanced section</prompt>

      <parameters>

        <parameter>
          <name>e_methionine</name>
          <prompt lang="en">Change initial start codons to methionine</prompt>
          <type>
            <datatype>
              <class>Boolean</class>
            </datatype>
          </type>
          <vdef>
            <value>1</value>
          </vdef>
          <format>
            <code proglang="python">(" -nomethionine", "")[ bool(value) ]</code>
          </format>
          <argpos>6</argpos>
          <comment>
            <text lang="en">START codons at the beginning of protein products will usually code for Methionine, despite what the codon will code for  when it is internal to a protein. This qualifier sets all such  START codons to code for Methionine by default.</text>
          </comment>
        </parameter>

        <parameter>
          <name>e_circular</name>
          <prompt lang="en">Is the sequence circular</prompt>
          <type>
            <datatype>
              <class>Boolean</class>
            </datatype>
          </type>
          <vdef>
            <value>0</value>
          </vdef>
          <format>
            <code proglang="python">("", " -circular")[ bool(value) ]</code>
          </format>
          <argpos>7</argpos>
        </parameter>

        <parameter>
          <name>e_reverse</name>
          <prompt lang="en">Find orfs in the reverse sequence</prompt>
          <type>
            <datatype>
              <class>Boolean</class>
            </datatype>
          </type>
          <vdef>
            <value>1</value>
          </vdef>
          <format>
            <code proglang="python">(" -noreverse", "")[ bool(value) ]</code>
          </format>
          <argpos>8</argpos>
          <comment>
            <text lang="en">Set this to be false if you do not wish to find ORFs in the reverse complement of the sequence.</text>
          </comment>
        </parameter>

        <parameter>
          <name>e_flanking</name>
          <prompt lang="en">Number of flanking nucleotides to report</prompt>
          <type>
            <datatype>
              <class>Integer</class>
            </datatype>
          </type>
          <vdef>
            <value>100</value>
          </vdef>
          <format>
            <code proglang="python">("", " -flanking=" + str(value))[value is not None and value!=vdef]</code>
          </format>
          <argpos>9</argpos>
          <comment>
            <text lang="en">If you have chosen one of the options of the type of sequence to find that gives the flanking sequence around a STOP or  START codon, this allows you to set the number of nucleotides  either side of that codon to output. If the region of flanking  nucleotides crosses the start or end of the sequence, no output is  given for this codon.</text>
          </comment>
        </parameter>
      </parameters>
    </paragraph>

    <paragraph>
      <name>e_output</name>
      <prompt lang="en">Output section</prompt>

      <parameters>

        <parameter>
          <name>e_outseq</name>
          <prompt lang="en">Name of the output sequence file (e_outseq)</prompt>
          <type>
            <biotype>Protein</biotype>
            <datatype>
              <class>Filename</class>
            </datatype>
          </type>
          <vdef>
            <value>outseq.orf</value>
          </vdef>
          <format>
            <code proglang="python">("" , " -outseq=" + str(value))[value is not None]</code>
          </format>
          <argpos>10</argpos>
        </parameter>

        <parameter>
          <name>e_osformat_outseq</name>
          <prompt lang="en">Choose the sequence output format</prompt>
          <type>
            <biotype>Protein</biotype>
            <datatype>
              <class>Choice</class>
            </datatype>
          </type>
          <vdef>
            <value>FASTA</value>
          </vdef>
          <vlist>
            <velem>
              <value>EMBL</value>
              <label>Embl</label>
            </velem>
            <velem>
              <value>FASTA</value>
              <label>Fasta</label>
            </velem>
            <velem>
              <value>GCG</value>
              <label>Gcg</label>
            </velem>
            <velem>
              <value>GENBANK</value>
              <label>Genbank</label>
            </velem>
            <velem>
              <value>NBRF</value>
              <label>Nbrf</label>
            </velem>
            <velem>
              <value>CODATA</value>
              <label>Codata</label>
            </velem>
            <velem>
              <value>RAW</value>
              <label>Raw</label>
            </velem>
            <velem>
              <value>SWISSPROT</value>
              <label>Swissprot</label>
            </velem>
            <velem>
              <value>GFF</value>
              <label>Gff</label>
            </velem>
          </vlist>
          <format>
            <code proglang="python">("", " -osformat=" + str(value))[value is not None and value!=vdef]</code>
          </format>
          <argpos>11</argpos>
        </parameter>

        <parameter isout="1">
          <name>e_outseq_out</name>
          <prompt lang="en">outseq_out option</prompt>
          <type>
            <biotype>Protein</biotype>
            <datatype>
              <class>Sequence</class>
            </datatype>
            <dataFormat>
              <ref param="e_osformat_outseq">
              </ref>
            </dataFormat>
          </type>
          <filenames>
            <code proglang="python">e_outseq</code>
          </filenames>
        </parameter>
      </parameters>
    </paragraph>

    <parameter ishidden="1">
      <name>auto</name>
      <prompt lang="en">Turn off any prompting</prompt>
      <type>
        <datatype>
          <class>String</class>
        </datatype>
      </type>
      <format>
        <code proglang="python">" -auto -stdout"</code>
      </format>
      <argpos>12</argpos>
    </parameter>
  </parameters>
</program>