/usr/bin/altree-add-S is in altree 1.3.1-2+b1.
This file is owned by root:root, with mode 0o755.
The actual contents of the file can be viewed below.
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96 97 98 99 100 101 102 103 104 105 106 107 108 109 110 111 112 113 114 115 116 117 118 119 120 121 122 123 124 125 126 127 128 129 130 131 132 133 134 135 136 137 138 139 140 141 142 143 144 145 146 147 148 149 150 151 152 153 154 155 156 157 158 159 160 161 162 163 164 165 166 167 168 169 170 171 172 173 174 175 176 177 178 179 180 181 182 183 184 185 186 187 188 189 190 191 192 193 194 195 196 197 198 199 200 201 202 203 204 205 206 207 208 209 210 211 212 213 214 215 216 217 218 219 220 221 222 223 224 225 226 227 228 229 230 231 232 233 234 235 236 237 238 239 240 241 242 243 244 245 246 247 248 249 250 251 252 253 254 255 256 257 258 259 260 261 262 263 264 265 266 267 268 269 270 271 272 273 274 275 276 277 278 279 280 281 282 283 284 285 286 287 288 289 290 291 292 293 294 295 296 297 298 299 300 301 302 303 304 305 306 307 308 309 310 311 312 313 314 315 316 317 318 319 320 321 322 323 324 325 326 327 328 329 330 331 332 333 334 335 336 337 338 339 340 341 342 343 344 345 346 347 348 349 350 351 352 353 354 355 356 357 358 359 360 361 362 363 364 365 366 367 368 369 370 371 372 373 374 375 376 377 378 379 380 381 382 383 384 385 386 387 388 389 390 391 392 393 394 395 396 397 398 399 400 401 402 403 404 405 406 407 408 409 410 411 412 413 414 415 416 417 418 419 420 421 422 423 424 425 426 427 428 429 430 431 432 433 434 435 436 437 438 439 440 441 442 443 444 445 446 447 448 449 450 451 452 453 454 455 456 457 458 459 460 461 462 463 464 465 466 467 468 469 470 471 472 473 474 475 476 477 478 479 480 481 482 483 484 485 486 487 488 489 490 491 492 493 494 495 496 497 498 499 500 501 502 503 504 505 506 507 508 509 510 511 512 513 514 515 516 517 518 519 520 521 522 523 524 525 526 527 528 529 530 531 532 533 534 535 536 537 538 539 540 541 542 543 544 545 546 547 548 549 550 551 552 553 554 555 556 557 558 559 560 561 562 563 564 565 566 567 568 569 570 571 572 573 574 575 | #!/usr/bin/perl
eval 'exec /usr/bin/perl -S $0 ${1+"$@"}'
if 0; # not running under some shell
#Ce programme etiquette les haplotypes malades (ajout d'un G) ou témoins (ajout d'un C), en fonction d'un seuil de malade et témoins qui le porte.
#Le programme prend en entrée un fichier .paup, et redonne un autre .paup
use strict;
use diagnostics;
use warnings;
use Getopt::Long; # qw(:config permute);
use Pod::Usage;
#use Getopt::Std;
our($opt_h,$opt_i, $opt_o, $opt_e, $opt_p, $opt_t, $opt_l, $opt_j,
$opt_g, $opt_q);
our $VERSION;
$VERSION = sprintf "0.%03d", q$Revision: 427 $ =~ /(\d+)/g;
sub DefineAncDer {
my $data_type=shift;
if ($data_type == 0) {
my $tem=0;
my $mal=1;
return ($tem, $mal);
} elsif ($data_type == 1) {
my $tem="C";
my $mal="G";
return ($tem, $mal);
}
}
sub ReadCorrespond
{
my($name_correspond) =shift;
my $data_qual = shift;
my($ligne, @tableau);
my(%correspondance);
open (CORRESP, '<', $name_correspond) || die "Unable to open file $name_correspond: $!\n";
while ($ligne=<CORRESP>) {
chomp($ligne);
if ($ligne =~ /^$/) {
next;
}
####### Qualitative data ######
if ($data_qual eq "quali") {
@tableau=split(/\s+/, $ligne);
if ($#tableau != 2) {
die "error in $name_correspond: not 3 columns at line '$ligne'\n";
} else {
$tableau[2]=~ s/c//;
$tableau[1]=~ s/m//;
if ($tableau[1] =~ /c/ || $tableau[2] =~ /m/) {
die "You have probably exchanged the order of cases and controls in file $name_correspond. It should be: haplo_name m_case_number c_control_number\n";
}
$correspondance{$tableau[0]}->{"case"}=$tableau[1]+0;
$correspondance{$tableau[0]}->{"control"}=$tableau[2]+0;
}
} else {
##### Quantitative data #######
@tableau = split(/\s+/, $ligne);
my @quant_val = splice(@tableau, 1);
$correspondance{$tableau[0]}=\@quant_val;
}
}
#my($clefs);
#DEBUG
# foreach $clefs (keys %correspondance) {
# print "$clefs case: ", $correspondance{$clefs}->{"case"}, "\n";
# print "$clefs, control: ",$correspondance{$clefs}->{"control"}, "\n";
# }
return(\%correspondance);
}
sub calcul_moyenne_variance
{
my $correspondance = shift;
my $somme=0;
my $nb_val=0;
my $somme_carres=0;
foreach my $haplo (keys %{$correspondance}) {
foreach my $elem (@{$correspondance->{$haplo}}) {
$nb_val++;
$somme+=$elem;
$somme_carres+=$elem*$elem;
}
}
my $moyenne = $somme/$nb_val;
my $variance = $somme_carres/$nb_val-$moyenne*$moyenne;
return ($moyenne, sqrt($variance));
}
sub calcul_moyenne_nbind
{
my $tableau = shift;
my $somme = 0;
my $nb_ind=scalar(@{$tableau});
foreach my $elem (@{$tableau}) {
$somme+=$elem;
}
return ($somme/$nb_ind, $nb_ind);
}
sub test_quanti_bilateral
{
my $moy_gen = shift;
my $ec_type_gen = shift;
my $moy_loc = shift;
my $nb_ind_loc = shift;
my $epsilon = shift;
if ($moy_loc > ($moy_gen + $epsilon*$ec_type_gen/sqrt($nb_ind_loc))) {
return "eleve";
} elsif ($moy_loc < ($moy_gen - $epsilon*$ec_type_gen/sqrt($nb_ind_loc))) {
return "faible";
} else {
return "?";
}
}
sub travail
{
my($seuil)=shift;
my($data_type)=shift;
my($proportion_malades)=shift;
my $low =shift;
my $name_correspond=shift;
my $outgroup = shift;
my $data_qual = shift;
my($ligne);
my($temoin, $malade, $sequence, $nom, $debut, $ancetre);
my($anc, $num_car, $prop_mal, $prop_tem);
my(@tableau, @tab2);
my($tem, $mal)=DefineAncDer($data_type);
my($correspondance)=ReadCorrespond($name_correspond,$data_qual);
#foreach my $clefs (keys %{$correspondance}) {
# print "$clefs case ", $correspondance->{$clefs}->{"case"}, "\n";
# print "$clefs, control ",$correspondance->{$clefs}->{"control"}, "\n";
# }
my $found_outgroup=0;
my $ici = 0;
my $phylo_prog= "Phylip";
my $compteur=0;
while ($ligne=<STDIN>){
chomp($ligne);
if ($ligne =~/^$/) {
next;
}
$compteur++;
my $diese='#';
if ($ligne =~ /^\s*$diese[N|n]exus\s*$/) {
$phylo_prog="PAUP";
}
if ($phylo_prog eq "PAUP") {
if ($ligne =~ /^\s*matrix\s*$/) {
print $ligne, "\n";
$ici=1;
next
}
if ($ligne =~ /^\s*;\s*$/) {
$ici=0;
}
if ($ligne =~ /^\s*\[/) {
next;
}
} else {
if ($compteur>1) {
$ici=1;
}
}
if ($ici==0 && $phylo_prog eq "PAUP") {
if ($ligne =~ /dimension ntax=([0-9]+)\s+nchar=([0-9]+);/) {
$num_car=$2+1;
print "dimension ntax=$1 nchar=", $num_car, ";\n";
}elsif ($ligne =~ /format symbols=\"([0-9ATGCU]+)\"/) {
my($format)=$1;
my($format_old)=$1;
$format =~ tr/GC//d;
$ligne =~ s/$format_old/${format}CG/;
print $ligne, "\n";
} elsif ($ligne =~ /ancstates\s+\*anc\s+vector\s*=\s*([0-9ATCG]+)\s*;/) {
$anc=$1;
$anc=$anc."?";
$ligne =~ s/$1/$anc/;
print $ligne,"\n";
} elsif ($ligne=~ /begin paup;/) {
print $ligne,"\n";
print "exclude $num_car; \n";
} elsif ($ligne=~ /\s*describetrees/) {
print "include $num_car;\n";
print $ligne,"\n";
} elsif ($ligne =~ /^\s*([0-9]+)\s+([0-9]+)$/) {
$num_car=$2+1;
print "$1\t$num_car\n";
} else {
print $ligne, "\n";
}
} elsif ($ici==0 && $phylo_prog eq "Phylip") {
if ($ligne =~ /^\s*([0-9]+)\s+([0-9]+)\s*$/) {
print $1, "\t", $2+1, "\n";
} else {
die "Strange line $ligne in Phylip file\n";
}
} elsif ($ici==1) {
if ($ligne =~ /^\s+$/) {
next;
} elsif ($ligne =~ /^\s*\[.+\]$/) {
# print STDERR "TTTTT\n";
next;
} else {
@tableau=split(/\s+/, $ligne);
$sequence=$tableau[1];
$nom=$tableau[0];
#DEBUG print "$nom $outgroup\n";
if ($nom eq $outgroup){
# $ancetre=$sequence."?";
print "$nom $sequence?\n";
$found_outgroup++;
next;
}
# } else {
#if ($debut =~ /^\s*H[0-9]{3}_m[0-9]{3}_c[0-9]{3}/) {
# @tab2=split(/_/,$debut);
# print $tab2[0],"\n";
# $temoin=$tab2[2];
# $temoin =~ s/c//;
# print STDERR "temoin=$temoin\n";
# $malade=$tab2[1];
# $malade =~ s/m//;
# if ($malade =~ /c/ || $temoin =~ /m/) {
# die "You have probably interverti cases and controls in file correspond.txt. It should be: haplo_name m_case_number c_control_number\n";
# }
# if ($debut eq $anc_name){
# $found_anc++;
# }
#$nom=$tableau[0];
# print "$nom\n";
###### QUALITATIVE ######
if ($data_qual eq "quali") {
if (not exists ($correspondance->{$nom})){
print STDERR "$nom is not found in the file $name_correspond. Assuming it is the outgroup.\nThe number of cases and controls affected to this sequence is set to 0\n";
$correspondance->{$nom}->{"case"}=0;
$correspondance->{$nom}->{"control"}=0;
}
$malade=$correspondance->{$nom}->{"case"};
$temoin=$correspondance->{$nom}->{"control"};
if ($malade == 0 && $temoin == 0) {
print STDERR "$nom is carried by 0 cases and 0 controls. The state ? has been attributed to the S character\n";
$sequence.="?";
print "$nom ", $sequence, "\n";
next;
}
# print " $nom mal=$malade\n";
$prop_mal=$malade/($malade+$temoin);
$prop_tem=$temoin/($malade+$temoin);
# print "M=$malade T=$temoin\n";
#print "test=$test\n";
# if ($test==0) { # test: difference |mal-tem| >=seuil
# if ($malade > $temoin && $malade-$temoin>=$seuil) {
#$sequence.="G";
# } elsif ($malade < $temoin && $temoin-$malade>=$seuil) {
# $sequence.="C";
# } else {
# $sequence.="?";
# }
#} elsif ($test==1) {
# if ($malade+$temoin==1) {
#$sequence.="?";
if ( $malade+$temoin <= $low) {
$sequence.="?";
} else {
if ($prop_mal>$proportion_malades+
$seuil*sqrt($prop_mal*$prop_tem/($malade+$temoin))) {
$sequence.=$mal;
} elsif ($prop_mal<$proportion_malades-
$seuil*sqrt($prop_mal*$prop_tem/($malade+$temoin))) {
$sequence.=$tem;
} else {
$sequence.="?";
}
#}
}
print "$nom ", $sequence, "\n";
#"_m$malade", "_c$temoin\t", $sequence, "\n";
} else {
###### QUANTITATIVE #######
if (not exists ($correspondance->{$nom})){
print STDERR "$nom is not found in the file".
"$name_correspond\n";
}
my ($moyenne_gen, $ec_type) = calcul_moyenne_variance
($correspondance);
my ($moyenne_loc, $nb_ind)= calcul_moyenne_nbind
($correspondance->{$nom});
#DEBUG print "$nom\n";
#DEBUG print "Moyenne: $moyenne_gen\n";
#DEBUG print "Variance: $ec_type\n";
#DEBUG print "Moyenne locale: $moyenne_loc\n";
#DEBUG print "nb_ind: $nb_ind\n";
if ($nb_ind <= $low) {
$sequence.="?";
} else {
if (test_quanti_bilateral($moyenne_gen, $ec_type, $moyenne_loc, $nb_ind, $seuil) eq "eleve") {
$sequence.=$mal;
} elsif (test_quanti_bilateral($moyenne_gen, $ec_type, $moyenne_loc, $nb_ind, $seuil) eq "faible") {
$sequence.=$tem;
} else {
$sequence.="?";
}
}
print "$nom ", $sequence, "\n";
}
}
}
}
#print "anc? $found_anc\n";
if ($found_outgroup==0 && $outgroup ne "nooutgroup") {
die "outgroup not found in the file\n";
} elsif ($found_outgroup ==1 && $outgroup eq "noanc") {
die " false outgroup found\n";
} elsif ($found_outgroup > 1) {
die "Too many outgroups found ($found_outgroup outgroup)";
}
}
sub usage {
my $msg =shift;
my($progname) =shift;
print STDERR "Error! ".$msg;
print STDERR "usage :$progname [options]\n";
print STDERR " Options :\n";
print STDERR " [-h] this help\n";
print STDERR " -i input file\n";
print STDERR " -j input2 file (correspond.txt)\n";
print STDERR " -o output file\n";
print STDERR " -t data type: SNP or DNA\n";
# ancienne option -t test: 0= mal-tem>seuil 1= seuil proportion0+/-sqr(pq/n)]
print STDERR " -p proportion of cases in the whole data set\n";
print STDERR " -e epsilon parameter\n";
print STDERR " -q quantitative or qualitative data\n";
print STDERR " [-g] name of the outgroup\n";
print STDERR " -l if an haplotype is present equal or less than -l times, the state of S will be set to ?\n";
}
sub main
{
my($progname);
my($seuil, $test, $proportion);
my %options= (
"first-input-file" => \$opt_i,
"second-input-file" => \$opt_j,
"output-file" => \$opt_o,
"epsilon" => \$opt_e,
"data-type" => \$opt_t,
"proportion" => \$opt_p,
"outgroup" => \$opt_g,
"low" => \$opt_l,
"data-qual" => \$opt_q,
);
#getopts('ho:i:j:e:t:p:l');
GetOptions (\%options,
"version",
"short-help|h",
"help",
"man",
"first-input-file|i=s",
"second-input-file|j=s",
"output-file|o=s",
"epsilon|e=s",
"data-type|t=s",
"proportion|p=s",
"outgroup|g=s",
"low|l=i",
"data-qual|q=s",
) or pod2usage(2);
if (defined($options{"version"})) {
print $0, " version ", $VERSION, "\n";
print "(Perl version ", $], ")\n";
exit 0;
}
if (defined($options{"short-help"})) {
pod2usage(-exitstatus => 0, -verbose => 0);
}
if (defined($options{"help"})) {
pod2usage(-exitstatus => 0, -verbose => 1);
}
if (defined($options{"man"})) {
pod2usage(-exitstatus => 0, -verbose => 2);
}
if ($opt_i) {
open(STDIN, '<', $opt_i) or die "Impossible to open $opt_i : $!" ;
}
my $correspond_name;
if ($opt_j) {
$correspond_name=$opt_j;
} else {
$correspond_name="correspond.txt"
}
if ($opt_o) {
open(STDOUT, '>', $opt_o) or die "Impossible to open $opt_o : $!" ;
}
if ($opt_e) {
$seuil=$opt_e;
} else {
usage("The epsilon parameter is not defined!!\n", $progname);
}
my($data_type);
if (defined($opt_t)) {
if ($opt_t =~ /[Dd][Nn][Aa]/) {
$data_type=1;
} elsif ($opt_t =~ /[Ss][Nn][Ps]/) {
$data_type=0;
} else {
usage("The data type (SNP or DNA) is missing\n", $progname);
}
# if (defined($opt_t)) {
# if ($opt_t==0) {
# $test=0;
# } elsif ($opt_t==1) {
# $test=1;
# } else {
# $test=-999;
# print STDERR "illegal value of opt_t\n";
# }
# } else {
# die "manque le numero du test: 0: mal-tem=seuil 1: seuil=P0+/-sqr(pq/n)!!\n";
# }
my($low);
# Si $low !=0, if only one case or one control, then the state of S is ?
if (not defined $opt_l) {
die "The minimum number of haplotype should be set by -l\n";
} else {
$low=$opt_l;
}
my $data_qual;
if (!$opt_q) {
die "data-qual must be specified\n";
} elsif ($opt_q eq "qualitative") {
$data_qual="quali";
} elsif ($opt_q eq "quantitative") {
$data_qual="quanti";
} else {
die "Invalid value for data-qual (opt_q)\n";
}
if ($data_qual eq "quali") {
if ($opt_p) {
$proportion=$opt_p;
} else {
usage("The proportion of cases in the sample is missing!\n", $progname);
}
}
my $outgroup="nooutgroup";
if ($opt_g) {
$outgroup = $opt_g;
}
travail($seuil, $data_type, $proportion, $low, $correspond_name, $outgroup, $data_qual);
}
}
main;
__END__
=head1 NAME
altree-add-S - Title...
=head1 SYNOPSIS
altree-add-S [options]
Options:
--version program version
--short-help|h brief help message
--help help message with options descriptions
--man full documentation
--first-input-file|i input file 1
--second-input-file|j input file 2 data concerning the trait (qualitative or quantitative)
--output-file|o output file
--epsilon|e epsilon value
--data-type|t data type: SNP or DNA
--proportion|p proportion of cases in the sample (for qualitative data only)
--data-qual|q data type: qualitative or quantitative
[--outgroup|g] name of the outgroup
--low|l if an haplotype is present equal or less than -l times, the state of S will be set to "?"
=head1 OPTIONS
=over 8
=item B<--version>
Print the program version and exits.
=item B<--short-help>
Print a brief help message and exits.
=item B<--help>
Print a help message with options descriptions and exits.
=item B<--man>
Prints the manual page and exits.
=item B<--first-input-file|i>
Input file 1 (paup or phylip file)
=item B<--second-input-file|j>
Input file 2, contains the number of times a given haplotypes is carried by case and control individuals (qualitative data) or the quantitative values correqsponding to a givent haplotype (quantitative data)
=item B<--output-file|o>
Output file
=item B<--epsilon|e>
espilon parameter (see formula in the documentation)
=item B<--data-type|t>
Type of data: DNA (ATGCU) or SNP (0-1)
=item B<--proportion|p>
Proportion of case individuals in the sample
=item B<--outgroup|g>
Name of the outgroup (if it is not in the file containing the number of cases and controls per haplotype)
=item B<--data-qual|q>
Specify if the data are qualitative or quantitative
=item B<--low|l>
if an haplotype is present equal or less than -l times, the state of S will be set to "?"
=back
=head1 DESCRIPTION
B<This program> adds a new character (called "character S") to each haplotype in the input file according to the number of cases and controls carrying it.
=cut
|