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# BioPerl module for Bio::Tree::Tree
#
# Please direct questions and support issues to <bioperl-l@bioperl.org>
#
# Cared for by Jason Stajich <jason@bioperl.org>
#
# Copyright Jason Stajich
#
# You may distribute this module under the same terms as perl itself
# POD documentation - main docs before the code
=head1 NAME
Bio::Tree::Tree - An implementation of the TreeI interface.
=head1 SYNOPSIS
use Bio::TreeIO;
# like from a TreeIO
my $treeio = Bio::TreeIO->new(-format => 'newick', -file => 'treefile.dnd');
my $tree = $treeio->next_tree;
my @nodes = $tree->get_nodes;
my $root = $tree->get_root_node;
=head1 DESCRIPTION
This object holds handles to Nodes which make up a tree.
=head1 IMPLEMENTATION NOTE
This implementation of Bio::Tree::Tree contains Bio::Tree:::NodeI; mainly linked
via the root node. As NodeI can potentially contain circular references (as
nodes will need to refer to both parent and child nodes), Bio::Tree::Tree will
remove those circular references when the object is garbage-collected. This has
some side effects; primarily, one must keep the Tree in scope or have at least
one reference to it if working with nodes. The fix is to count the references to
the nodes and if it is greater than expected retain all of them, but it requires
an additional prereq and thus may not be worth the effort. This only shows up
in minor edge cases, though (see Bug #2869).
Example of issue:
# tree is not assigned to a variable, so passes from memory after
# root node is passed
my $root = Bio::TreeIO->new(-format => 'newick', -file => 'foo.txt')->next_tree
->get_root_node;
# gets nothing, as all Node links are broken when Tree is garbage-collected above
my @descendents = $root->get_all_Descendents;
=head1 FEEDBACK
=head2 Mailing Lists
User feedback is an integral part of the evolution of this and other
Bioperl modules. Send your comments and suggestions preferably to
the Bioperl mailing list. Your participation is much appreciated.
bioperl-l@bioperl.org - General discussion
http://bioperl.org/wiki/Mailing_lists - About the mailing lists
=head2 Support
Please direct usage questions or support issues to the mailing list:
I<bioperl-l@bioperl.org>
rather than to the module maintainer directly. Many experienced and
reponsive experts will be able look at the problem and quickly
address it. Please include a thorough description of the problem
with code and data examples if at all possible.
=head2 Reporting Bugs
Report bugs to the Bioperl bug tracking system to help us keep track
of the bugs and their resolution. Bug reports can be submitted via
the web:
https://github.com/bioperl/bioperl-live/issues
=head1 AUTHOR - Jason Stajich
Email jason@bioperl.org
=head1 CONTRIBUTORS
Aaron Mackey amackey@virginia.edu
Sendu Bala bix@sendu.me.uk
Mark A. Jensen maj@fortinbras.us
=head1 APPENDIX
The rest of the documentation details each of the object methods.
Internal methods are usually preceded with a _
=cut
# Let the code begin...
package Bio::Tree::Tree;
use strict;
# Object preamble - inherits from Bio::Root::Root
use base qw(Bio::Root::Root Bio::Tree::TreeI Bio::Tree::TreeFunctionsI);
=head2 new
Title : new
Usage : my $obj = Bio::Tree::Tree->new();
Function: Builds a new Bio::Tree::Tree object
Returns : Bio::Tree::Tree
Args : -root => L<Bio::Tree::NodeI> object which is the root
OR
-node => L<Bio::Tree::NodeI> object from which the root will be
determined
-nodelete => boolean, whether or not to try and cleanup all
the nodes when this this tree goes out of scope.
-id => optional tree ID
-score => optional tree score value
=cut
sub new {
my ($class, @args) = @_;
my $self = $class->SUPER::new(@args);
$self->{'_rootnode'} = undef;
$self->{'_maxbranchlen'} = 0;
$self->_register_for_cleanup(\&cleanup_tree);
my ($root, $node, $nodel, $id, $score) =
$self->_rearrange([qw(ROOT NODE NODELETE ID SCORE)], @args);
if ($node && ! $root) {
$self->throw("Must supply a Bio::Tree::NodeI") unless ref($node) && $node->isa('Bio::Tree::NodeI');
my @lineage = $self->get_lineage_nodes($node);
$root = shift(@lineage) || $node;
# to stop us pulling in entire database of a Bio::Taxon when we later do
# get_nodes() or similar, specifically set ancestor() for each node
if ($node->isa('Bio::Taxon')) {
push(@lineage, $node) unless $node eq $root;
my $ancestor = $root;
foreach my $lineage_node (@lineage) {
$lineage_node->ancestor($ancestor);
} continue { $ancestor = $lineage_node; }
}
}
if ($root) {
$self->set_root_node($root);
}
$self->nodelete($nodel || 0);
$self->id($id) if defined $id;
$self->score($score) if defined $score;
return $self;
}
=head2 nodelete
Title : nodelete
Usage : $obj->nodelete($newval)
Function: Get/Set Boolean whether or not to delete the underlying
nodes when it goes out of scope. By default this is false
meaning trees are cleaned up.
Returns : boolean
Args : on set, new boolean value
=cut
sub nodelete {
my $self = shift;
return $self->{'nodelete'} = shift if @_;
return $self->{'nodelete'};
}
=head2 get_nodes
Title : get_nodes
Usage : my @nodes = $tree->get_nodes()
Function: Return list of Bio::Tree::NodeI objects
Returns : array of Bio::Tree::NodeI objects
Args : (named values) hash with one value
order => 'b|breadth' first order or 'd|depth' first order
sortby => [optional] "height", "creation", "alpha", "revalpha",
or coderef to be used to sort the order of children nodes. See L<Bio::Tree::Node> for details
=cut
sub get_nodes {
my ($self, @args) = @_;
my ($order, $sortby) = $self->_rearrange([qw(ORDER SORTBY)], @args);
$order ||= 'depth';
$sortby ||= 'none';
my @children;
my $node = $self->get_root_node;
if ($node) {
if ($order =~ m/^b/oi) { # breadth-first
@children = ($node);
my @to_process = ($node);
while( @to_process ) {
my $n = shift @to_process;
my @c = $n->each_Descendent($sortby);
push @children, @c;
push @to_process, @c;
}
} elsif ($order =~ m/^d/oi) { # depth-first
@children = ($node, $node->get_all_Descendents($sortby));
} else {
$self->verbose(1);
$self->warn("specified an order '$order' which I don't understan\n");
}
}
return @children;
}
=head2 get_root_node
Title : get_root_node
Usage : my $node = $tree->get_root_node();
Function: Get the Top Node in the tree, in this implementation
Trees only have one top node.
Returns : Bio::Tree::NodeI object
Args : none
=cut
sub get_root_node {
my ($self) = @_;
return $self->{'_rootnode'};
}
=head2 set_root_node
Title : set_root_node
Usage : $tree->set_root_node($node)
Function: Set the Root Node for the Tree
Returns : Bio::Tree::NodeI
Args : Bio::Tree::NodeI
=cut
sub set_root_node {
my $self = shift;
if ( @_ ) {
my $value = shift;
if ( defined $value && ! $value->isa('Bio::Tree::NodeI') ) {
$self->warn("Trying to set the root node to $value which is not a Bio::Tree::NodeI");
return $self->get_root_node;
}
$self->{'_rootnode'} = $value;
}
return $self->get_root_node;
}
=head2 total_branch_length
Title : total_branch_length
Usage : my $size = $tree->total_branch_length
Function: Returns the sum of the length of all branches
Returns : real
Args : none
=cut
sub total_branch_length { shift->subtree_length }
=head2 subtree_length
Title : subtree_length
Usage : my $subtree_size = $tree->subtree_length($internal_node)
Function: Returns the sum of the length of all branches in a subtree
under the node. Calculates the size of the whole tree
without an argument (but only if root node is defined)
Returns : real or undef
Args : Bio::Tree::NodeI object, defaults to the root node
=cut
sub subtree_length {
my $tree = shift;
my $node = shift || $tree->get_root_node;
return unless $node;
my $sum = 0;
for ( $node->get_all_Descendents ) {
$sum += $_->branch_length || 0;
}
return $sum;
}
=head2 id
Title : id
Usage : my $id = $tree->id();
Function: An id value for the tree
Returns : scalar
Args : [optional] new value to set
=cut
sub id {
my ($self, $val) = @_;
if ( defined $val ) {
$self->{'_treeid'} = $val;
}
return $self->{'_treeid'};
}
=head2 score
Title : score
Usage : $obj->score($newval)
Function: Sets the associated score with this tree
This is a generic slot which is probably best used
for log likelihood or other overall tree score
Returns : value of score
Args : newvalue (optional)
=cut
sub score {
my ($self, $val) = @_;
if ( defined $val ) {
$self->{'_score'} = $val;
}
return $self->{'_score'};
}
# decorated interface TreeI Implements this
=head2 height
Title : height
Usage : my $height = $tree->height
Function: Gets the height of tree - this LOG_2($number_nodes)
WARNING: this is only true for strict binary trees. The TreeIO
system is capable of building non-binary trees, for which this
method will currently return an incorrect value!!
Returns : integer
Args : none
=head2 number_nodes
Title : number_nodes
Usage : my $size = $tree->number_nodes
Function: Returns the number of nodes in the tree
Returns : integer
Args : none
=head2 as_text
Title : as_text
Usage : my $tree_as_string = $tree->as_text($format)
Function: Returns the tree as a string representation in the
desired format, e.g.: 'newick', 'nhx' or 'tabtree' (the default)
Returns : scalar string
Args : format type as specified by Bio::TreeIO
Note : This method loads the Bio::TreeIO::$format module
on the fly, and commandeers the _write_tree_Helper
routine therein to create the tree string.
=cut
sub as_text {
my $self = shift;
my $format = shift || 'tabtree';
my $params_input = shift || {};
my $iomod = "Bio::TreeIO::$format";
$self->_load_module($iomod);
my $string = '';
open my $fh, '>', \$string or $self->throw("Could not write '$string' as file: $!");
my $test = $iomod->new( -format => $format, -fh => $fh );
# Get the default params for the given IO module.
$test->set_params($params_input);
$test->write_tree($self);
close $fh;
return $string;
}
=head2 Methods for associating Tag/Values with a Tree
These methods associate tag/value pairs with a Tree
=head2 set_tag_value
Title : set_tag_value
Usage : $tree->set_tag_value($tag,$value)
$tree->set_tag_value($tag,@values)
Function: Sets a tag value(s) to a tree. Replaces old values.
Returns : number of values stored for this tag
Args : $tag - tag name
$value - value to store for the tag
=cut
sub set_tag_value {
my ($self, $tag, @values) = @_;
if ( ! defined $tag || ! scalar @values ) {
$self->warn("cannot call set_tag_value with an undefined value");
}
$self->remove_tag ($tag);
map { push @{$self->{'_tags'}->{$tag}}, $_ } @values;
return scalar @{$self->{'_tags'}->{$tag}};
}
=head2 add_tag_value
Title : add_tag_value
Usage : $tree->add_tag_value($tag,$value)
Function: Adds a tag value to a tree
Returns : number of values stored for this tag
Args : $tag - tag name
$value - value to store for the tag
=cut
sub add_tag_value {
my ($self, $tag, $value) = @_;
if ( ! defined $tag || ! defined $value ) {
$self->warn("cannot call add_tag_value with an undefined value");
}
push @{$self->{'_tags'}->{$tag}}, $value;
return scalar @{$self->{'_tags'}->{$tag}};
}
=head2 remove_tag
Title : remove_tag
Usage : $tree->remove_tag($tag)
Function: Remove the tag and all values for this tag
Returns : boolean representing success (0 if tag does not exist)
Args : $tag - tagname to remove
=cut
sub remove_tag {
my ($self, $tag) = @_;
if ( exists $self->{'_tags'}->{$tag} ) {
$self->{'_tags'}->{$tag} = undef;
delete $self->{'_tags'}->{$tag};
return 1;
}
return 0;
}
=head2 remove_all_tags
Title : remove_all_tags
Usage : $tree->remove_all_tags()
Function: Removes all tags
Returns : None
Args : None
=cut
sub remove_all_tags {
my ($self) = @_;
$self->{'_tags'} = {};
return;
}
=head2 get_all_tags
Title : get_all_tags
Usage : my @tags = $tree->get_all_tags()
Function: Gets all the tag names for this Tree
Returns : Array of tagnames
Args : None
=cut
sub get_all_tags {
my ($self) = @_;
my @tags = sort keys %{$self->{'_tags'} || {}};
return @tags;
}
=head2 get_tag_values
Title : get_tag_values
Usage : my @values = $tree->get_tag_values($tag)
Function: Gets the values for given tag ($tag)
Returns : Array of values or empty list if tag does not exist
Args : $tag - tag name
=cut
sub get_tag_values {
my ($self, $tag) = @_;
return wantarray ? @{$self->{'_tags'}->{$tag} || []} :
(@{$self->{'_tags'}->{$tag} || []})[0];
}
=head2 has_tag
Title : has_tag
Usage : $tree->has_tag($tag)
Function: Boolean test if tag exists in the Tree
Returns : Boolean
Args : $tag - tagname
=cut
sub has_tag {
my ($self, $tag) = @_;
return exists $self->{'_tags'}->{$tag};
}
# safe tree clone that doesn't seg fault
=head2 clone
Title : clone
Alias : _clone
Usage : $tree_copy = $tree->clone();
$subtree_copy = $tree->clone($internal_node);
Function: Safe tree clone that doesn't segfault
Returns : Bio::Tree::Tree object
Args : [optional] $start_node, Bio::Tree::Node object
=cut
sub clone {
my ($self, $parent, $parent_clone) = @_;
$parent ||= $self->get_root_node;
$parent_clone ||= $self->_clone_node($parent);
foreach my $node ($parent->each_Descendent()) {
my $child = $self->_clone_node($node);
$child->ancestor($parent_clone);
$self->_clone($node, $child);
}
$parent->ancestor && return;
my $tree = $self->new(-root => $parent_clone);
return $tree;
}
# -- private internal methods --
sub cleanup_tree {
my $self = shift;
unless( $self->nodelete ) {
for my $node ($self->get_nodes(-order => 'b', -sortby => 'none')) {
$node->node_cleanup;
}
}
$self->{'_rootnode'} = undef;
}
1;
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