/usr/bin/bp_bulk_load_gff is in bioperl 1.6.924-1.
This file is owned by root:root, with mode 0o755.
The actual contents of the file can be viewed below.
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96 97 98 99 100 101 102 103 104 105 106 107 108 109 110 111 112 113 114 115 116 117 118 119 120 121 122 123 124 125 126 127 128 129 130 131 132 133 134 135 136 137 138 139 140 141 142 143 144 145 146 147 148 149 150 151 152 153 154 155 156 157 158 159 160 161 162 163 164 165 166 167 168 169 170 171 172 173 174 175 176 177 178 179 180 181 182 183 184 185 186 187 188 189 190 191 192 193 194 195 196 197 198 199 200 201 202 203 204 205 206 207 208 209 210 211 212 213 214 215 216 217 218 219 220 221 222 223 224 225 226 227 228 229 230 231 232 233 234 235 236 237 238 239 240 241 242 243 244 245 246 247 248 249 250 251 252 253 254 255 256 257 258 259 260 261 262 263 264 265 266 267 268 269 270 271 272 273 274 275 276 277 278 279 280 281 282 283 284 285 286 287 288 289 290 291 292 293 294 295 296 297 298 299 300 301 302 303 304 305 306 307 308 309 310 311 312 313 314 315 316 317 318 319 320 321 322 323 324 325 326 327 328 329 330 331 332 333 334 335 336 337 338 339 340 341 342 343 344 345 346 347 348 349 350 351 352 353 354 355 356 357 358 359 360 361 362 363 364 365 366 367 368 369 370 371 372 373 374 375 376 377 378 379 380 381 382 383 384 385 386 387 388 389 390 391 392 393 394 395 396 397 398 399 400 401 402 403 404 405 406 407 408 409 410 411 412 413 414 415 416 417 418 419 420 421 422 423 424 425 426 427 428 429 430 431 432 433 434 435 436 437 438 439 440 441 442 443 444 445 446 447 448 449 450 451 452 453 454 455 456 457 458 459 460 461 462 463 464 465 466 467 468 469 470 471 472 473 474 475 476 477 478 479 480 481 482 483 484 485 486 487 488 489 490 491 492 493 494 495 496 497 498 499 500 501 502 503 504 505 506 507 508 509 510 511 512 513 514 515 516 517 518 519 520 521 522 523 524 525 526 527 528 529 530 531 532 533 534 535 536 537 538 539 540 541 542 543 544 545 546 547 548 549 550 551 552 553 554 555 556 557 558 559 560 561 562 563 564 565 566 567 568 569 570 571 572 573 574 575 576 577 578 579 580 581 582 583 584 585 586 587 588 589 590 591 592 593 594 595 596 597 598 599 600 601 602 603 604 605 606 607 608 609 610 611 612 613 614 615 616 617 618 619 620 621 622 623 624 625 626 627 628 629 630 631 632 633 634 635 636 637 638 639 640 641 642 643 644 645 646 647 648 649 650 651 652 653 654 655 656 657 658 659 660 661 662 663 664 665 666 667 668 669 670 671 672 673 674 675 676 677 678 679 680 681 682 683 684 685 686 687 688 689 690 691 692 693 694 695 696 697 698 699 700 701 | #!/usr/bin/perl
eval 'exec /usr/bin/perl -S $0 ${1+"$@"}'
if 0; # not running under some shell
use strict;
use warnings;
# use lib './blib/lib';
use DBI;
use IO::File;
use File::Spec;
use Getopt::Long;
use Bio::DB::GFF;
use Bio::DB::GFF::Util::Binning 'bin';
use constant MYSQL => 'mysql';
use constant FDATA => 'fdata';
use constant FTYPE => 'ftype';
use constant FGROUP => 'fgroup';
use constant FDNA => 'fdna';
use constant FATTRIBUTE => 'fattribute';
use constant FATTRIBUTE_TO_FEATURE => 'fattribute_to_feature';
=head1 NAME
bp_bulk_load_gff.pl - Bulk-load a Bio::DB::GFF database from GFF files.
=head1 SYNOPSIS
% bp_bulk_load_gff.pl -d testdb dna1.fa dna2.fa features1.gff features2.gff ...
=head1 DESCRIPTION
This script loads a Bio::DB::GFF database with the features contained
in a list of GFF files and/or FASTA sequence files. You must use the
exact variant of GFF described in L<Bio::DB::GFF>. Various
command-line options allow you to control which database to load and
whether to allow an existing database to be overwritten.
This script differs from bp_load_gff.pl in that it is hard-coded to use
MySQL and cannot perform incremental loads. See L<bp_load_gff.pl> for an
incremental loader that works with all databases supported by
Bio::DB::GFF, and L<bp_fast_load_gff.pl> for a MySQL loader that supports
fast incremental loads.
=head2 NOTES
If the filename is given as "-" then the input is taken from standard
input. Compressed files (.gz, .Z, .bz2) are automatically
uncompressed.
FASTA format files are distinguished from GFF files by their filename
extensions. Files ending in .fa, .fasta, .fast, .seq, .dna and their
uppercase variants are treated as FASTA files. Everything else is
treated as a GFF file. If you wish to load -fasta files from STDIN,
then use the -f command-line swith with an argument of '-', as in
gunzip my_data.fa.gz | bp_fast_load_gff.pl -d test -f -
The nature of the bulk load requires that the database be on the local
machine and that the indicated user have the "file" privilege to load
the tables and have enough room in /usr/tmp (or whatever is specified
by the \$TMPDIR environment variable), to hold the tables transiently.
Local data may now be uploaded to a remote server via the --local option
with the database host specified in the dsn, e.g. dbi:mysql:test:db_host
The adaptor used is dbi::mysqlopt. There is currently no way to
change this.
About maxfeature: the default value is 100,000,000 bases. If you have
features that are close to or greater that 100Mb in length, then the
value of maxfeature should be increased to 1,000,000,000. This value
must be a power of 10.
Note that Windows users must use the --create option.
If the list of GFF or fasta files exceeds the kernel limit for the
maximum number of command-line arguments, use the
--long_list /path/to/files option.
=head1 COMMAND-LINE OPTIONS
Command-line options can be abbreviated to single-letter options.
e.g. -d instead of --database.
--database <dsn> Database name (default dbi:mysql:test)
--adaptor Adaptor name (default mysql)
--create Reinitialize/create data tables without asking
--user Username to log in as
--fasta File or directory containing fasta files to load
--long_list Directory containing a very large number of
GFF and/or FASTA files
--password Password to use for authentication
(Does not work with Postgres, password must be
supplied interactively or be left empty for
ident authentication)
--maxbin Set the value of the maximum bin size
--local Flag to indicate that the data source is local
--maxfeature Set the value of the maximum feature size (power of 10)
--group A list of one or more tag names (comma or space separated)
to be used for grouping in the 9th column.
--gff3_munge Activate GFF3 name munging (see Bio::DB::GFF)
--summary Generate summary statistics for drawing coverage histograms.
This can be run on a previously loaded database or during
the load.
--Temporary Location of a writable scratch directory
=head1 SEE ALSO
L<Bio::DB::GFF>, L<fast_load_gff.pl>, L<load_gff.pl>
=head1 AUTHOR
Lincoln Stein, lstein@cshl.org
Copyright (c) 2002 Cold Spring Harbor Laboratory
This library is free software; you can redistribute it and/or modify
it under the same terms as Perl itself. See DISCLAIMER.txt for
disclaimers of warranty.
=cut
package Bio::DB::GFF::Adaptor::fauxmysql;
use Bio::DB::GFF::Adaptor::dbi::mysqlopt;
use vars '@ISA';
@ISA = 'Bio::DB::GFF::Adaptor::dbi::mysqlopt';
sub insert_sequence {
my $self = shift;
my ($id,$offset,$seq) = @_;
print join("\t",$id,$offset,$seq),"\n";
};
package Bio::DB::GFF::Adaptor::fauxmysqlcmap;
use Bio::DB::GFF::Adaptor::dbi::mysqlcmap;
use vars '@ISA';
@ISA = 'Bio::DB::GFF::Adaptor::dbi::mysqlcmap';
sub insert_sequence {
my $self = shift;
my ($id,$offset,$seq) = @_;
print join("\t",$id,$offset,$seq),"\n";
};
package Bio::DB::GFF::Adaptor::fauxpg;
use Bio::DB::GFF::Adaptor::dbi::pg;
use vars '@ISA';
@ISA = 'Bio::DB::GFF::Adaptor::dbi::pg';
#these two subs are to separate the table creation from the
#index creation
sub do_initialize {
my $self = shift;
my $erase = shift;
$self->drop_all if $erase;
my $dbh = $self->features_db;
my $schema = $self->schema;
foreach my $table_name ($self->tables) {
my $create_table_stmt = $schema->{$table_name}{table} ;
$dbh->do($create_table_stmt) || warn $dbh->errstr;
# $self->create_other_schema_objects(\%{$schema->{$table_name}});
}
1;
}
sub _create_indexes_etc {
my $self = shift;
my $dbh = $self->features_db;
my $schema = $self->schema;
foreach my $table_name ($self->tables) {
$self->create_other_schema_objects(\%{$schema->{$table_name}});
}
}
sub insert_sequence {
my $self = shift;
my ($id,$offset,$seq) = @_;
print "$id\t$offset\t$seq\n";
}
package main;
eval "use Time::HiRes"; undef $@;
my $timer = defined &Time::HiRes::time;
my $bWINDOWS = 0; # Boolean: is this a MSWindows operating system?
if ($^O =~ /MSWin32/i) {
$bWINDOWS = 1;
}
my ($DSN,$ADAPTOR,$FORCE,$USER,$PASSWORD,$FASTA,$LOCAL,$MAX_BIN,$GROUP_TAG,$LONG_LIST,$MUNGE,$TMPDIR);
GetOptions ('database:s' => \$DSN,
'adaptor:s' => \$ADAPTOR,
'create' => \$FORCE,
'user:s' => \$USER,
'password:s' => \$PASSWORD,
'fasta:s' => \$FASTA,
'local' => \$LOCAL,
'maxbin|maxfeature:s' => \$MAX_BIN,
'group:s' => \$GROUP_TAG,
'long_list:s' => \$LONG_LIST,
'gff3_munge' => \$MUNGE,
'Temporary:s' => \$TMPDIR,
) or (system('pod2text', $0), exit -1);
# If called as pg_bulk_load_gff.pl behave as that did.
if ($0 =~/pg_bulk_load_gff.pl/){
$ADAPTOR ||= 'Pg';
$DSN ||= 'test';
}
$DSN ||= 'dbi:mysql:test';
$MAX_BIN ||= 1_000_000_000; # to accomodate human-sized chromosomes
if ($bWINDOWS && not $FORCE) {
die "Note that Windows users must use the --create option.\n";
}
unless ($FORCE) {
die "This will delete all existing data in database $DSN. If you want to do this, rerun with the --create option.\n"
if $bWINDOWS;
open (TTY,"/dev/tty") or die "/dev/tty: $!\n"; #TTY use removed for win compatability
print STDERR "This operation will delete all existing data in database $DSN. Continue? ";
my $f = <TTY>;
die "Aborted\n" unless $f =~ /^[yY]/;
close TTY;
}
# postgres DBD::Pg allows 'database', but also 'dbname', and 'db':
# and it must be Pg (not pg)
$DSN=~s/pg:database=/Pg:/i;
$DSN=~s/pg:dbname=/Pg:/i;
$DSN=~s/pg:db=/Pg:/i;
# leave these lines for mysql
$DSN=~s/database=//i;
$DSN=~s/;host=/:/i; #cater for dsn in the form of "dbi:mysql:database=$dbname;host=$host"
my($DBI,$DBD,$DBNAME,$HOST)=split /:/,$DSN;
$DBNAME=$DSN unless $DSN=~/:/;
$ADAPTOR ||= $DBD;
$ADAPTOR ||= 'mysql';
if ($DBD eq 'Pg') {
# rebuild DSN, DBD::Pg requires full dbname=<name> format
$DSN = "dbi:Pg:dbname=$DBNAME";
if ($HOST) { $DSN .= ";host=$HOST"; }
}
my ($use_mysql,$use_mysqlcmap,$use_pg) = (0,0,0);
if ( $ADAPTOR eq 'mysqlcmap' ) {
$use_mysqlcmap = 1;
}
elsif ( $ADAPTOR =~ /^mysql/ ) {
$use_mysql = 1;
}
elsif ( $ADAPTOR eq "Pg" ) {
$use_pg = 1;
}
else{
die "$ADAPTOR is not an acceptable database adaptor.";
}
my (@auth,$AUTH);
if (defined $USER) {
push @auth,(-user=>$USER);
if ( $use_mysql or $use_mysqlcmap ) {
$AUTH .= " -u$USER";
}
elsif ( $use_pg ) {
$AUTH .= " -U $USER ";
}
}
if (defined $PASSWORD) {
push @auth,(-pass=>$PASSWORD);
if ( $use_mysql or $use_mysqlcmap ) {
$AUTH .= " -p$PASSWORD";
}
# elsif ( $use_pg ) {
# $AUTH .= " -W $PASSWORD ";
# }
}
if (defined $HOST) {
$AUTH .= " -h$HOST";
}
if (defined $DBNAME) {
if ( $use_mysql or $use_mysqlcmap ) {
$AUTH .= " -D$DBNAME ";
}
}
if (defined $LOCAL) {
$LOCAL='local';
$AUTH.=' --local-infile=1';
}else {
$LOCAL='';
}
my $faux_adaptor;
if ( $use_mysqlcmap ) {
$faux_adaptor = "fauxmysqlcmap";
}
elsif ( $use_mysql ) {
$faux_adaptor = "fauxmysql";
}
elsif ( $use_pg ) {
$faux_adaptor = "fauxpg";
}
my $db = Bio::DB::GFF->new(-adaptor=>$faux_adaptor,-dsn => $DSN,@auth)
or die "Can't open database: ",Bio::DB::GFF->error,"\n";
$db->gff3_name_munging(1) if $MUNGE;
$MAX_BIN ? $db->initialize(-erase=>1,-MAX_BIN=>$MAX_BIN) : $db->initialize(1);
$MAX_BIN ||= $db->meta('max_bin') || 100_000_000;
# deal with really long lists of files
if ($LONG_LIST) {
-d $LONG_LIST or die "The --long_list argument must be a directory\n";
opendir GFFDIR,$LONG_LIST or die "Could not open $LONG_LIST for reading: $!";
@ARGV = map { "$LONG_LIST\/$_" } readdir GFFDIR;
closedir GFFDIR;
if (defined $FASTA && -d $FASTA) {
opendir FASTA,$FASTA or die "Could not open $FASTA for reading: $!";
push @ARGV, map { "$FASTA\/$_" } readdir FASTA;
closedir FASTA;
}
elsif (defined $FASTA && -f $FASTA) {
push @ARGV, $FASTA;
}
}
foreach (@ARGV) {
$_ = "gunzip -c $_ |" if /\.gz$/;
$_ = "uncompress -c $_ |" if /\.Z$/;
$_ = "bunzip2 -c $_ |" if /\.bz2$/;
}
my (@gff,@fasta);
foreach (@ARGV) {
if (/\.(fa|fasta|dna|seq|fast)(?:$|\.)/i) {
push @fasta,$_;
} else {
push @gff,$_;
}
}
@ARGV = @gff;
push @fasta,$FASTA if defined $FASTA;
# drop everything that was there before
my %FH;
my $tmpdir = File::Spec->tmpdir() || '/tmp';
$tmpdir =~ s!\\!\\\\!g if $bWINDOWS; #eliminates backslash mis-interpretation
-d $tmpdir or die <<END;
I could not find a suitable temporary directory to write scratch files into ($tmpdir by default).
Please select a directory and indicate its location by setting the TMP environment variable, or
by using the --Temporary switch.
END
my @fasta_files_to_be_unlinked;
my @files = (FDATA,FTYPE,FGROUP,FDNA,FATTRIBUTE,FATTRIBUTE_TO_FEATURE);
foreach (@files) {
$FH{$_} = IO::File->new(">$tmpdir/$_.$$") or die $_,": $!";
$FH{$_}->autoflush;
}
if ( $use_pg ) {
$FH{FDATA() }->print("COPY fdata (fid, fref, fstart, fstop, fbin, ftypeid, fscore, fstrand, fphase, gid, ftarget_start, ftarget_stop) FROM stdin;\n");
$FH{FTYPE() }->print("COPY ftype (ftypeid, fmethod, fsource) FROM stdin;\n");
$FH{FGROUP() }->print("COPY fgroup (gid, gclass, gname) FROM stdin;\n");
$FH{FATTRIBUTE() }->print("COPY fattribute (fattribute_id, fattribute_name) FROM stdin;\n");
$FH{FATTRIBUTE_TO_FEATURE()}->print("COPY fattribute_to_feature (fid, fattribute_id, fattribute_value) FROM stdin;\n");
}
my $FID = 1;
my $GID = 1;
my $FTYPEID = 1;
my $ATTRIBUTEID = 1;
my %GROUPID = ();
my %FTYPEID = ();
my %ATTRIBUTEID = ();
my %DONE = ();
my $FEATURES = 0;
my %tmpfiles; # keep track of temporary fasta files
my $count;
my $fasta_sequence_id;
my $gff3;
my $current_file; #used to reset GFF3 flag in mix of GFF and GFF3 files
$db->preferred_groups(split (/[,\s]+/,$GROUP_TAG)) if defined $GROUP_TAG;
my $last = Time::HiRes::time() if $timer;
my $start = $last;
# avoid hanging on standalone --fasta load
if (!@ARGV) {
$FH{NULL} = IO::File->new(">$tmpdir/null");
push @ARGV, "$tmpdir/null";
}
my ($cmap_db);
if ($use_mysqlcmap){
my $options = {
AutoCommit => 1,
FetchHashKeyName => 'NAME_lc',
LongReadLen => 3000,
LongTruncOk => 1,
RaiseError => 1,
};
$cmap_db = DBI->connect( $DSN, $USER, $PASSWORD, $options );
}
# Only load CMap::Utils if using cmap
unless (!$use_mysqlcmap or
eval {
require Bio::GMOD::CMap::Utils;
Bio::GMOD::CMap::Utils->import('next_number');
1;
}
) {
print STDERR "Error loading Bio::GMOD::CMap::Utils\n";
}
while (<>) {
$current_file ||= $ARGV;
# reset GFF3 flag if new filehandle
unless($current_file eq $ARGV){
undef $gff3;
$current_file = $ARGV;
}
chomp;
my ($ref,$source,$method,$start,$stop,$score,$strand,$phase,$group);
# close sequence filehandle if required
if ( /^\#|\s+|^$|^>|\t/ && defined $FH{FASTA}) {
$FH{FASTA}->close;
delete $FH{FASTA};
}
# print to fasta file if the handle is open
if ( defined $FH{FASTA} ) {
$FH{FASTA}->print("$_\n");
next;
}
elsif (/^>(\S+)/) { # uh oh, sequence coming
$FH{FASTA} = IO::File->new(">$tmpdir/$1\.fa") or die "FASTA: $!\n";
$FH{FASTA}->print("$_\n");
print STDERR "Preparing embedded sequence $1\n";
push @fasta, "$tmpdir/$1\.fa";
push @fasta_files_to_be_unlinked,"$tmpdir/$1\.fa";
$tmpfiles{"$tmpdir/$1\.fa"}++;
next;
}
elsif (/^\#\#\s*gff-version\s+(\d+)/) {
$gff3 = ($1 >= 3);
$db->print_gff3_warning() if $gff3;
next;
}
elsif (/^\#\#\s*group-tags\s+(.+)/) {
$db->preferred_groups(split(/\s+/,$1));
next;
}
elsif (/^\#\#\s*sequence-region\s+(\S+)\s+(\d+)\s+(\d+)/i) { # header line
($ref,$source,$method,$start,$stop,$score,$strand,$phase,$group) =
($1,'reference','Component',$2,$3,'.','.','.',$gff3 ? "ID=Sequence:$1": qq(Sequence "$1"));
}
elsif (/^\#/) {
next;
}
else {
($ref,$source,$method,$start,$stop,$score,$strand,$phase,$group) = split "\t";
}
if ( not defined( $ref ) or length ($ref) == 0) {
warn "\$ref is null. source = $source, method = $method, group = $group\n";
next;
}
$FEATURES++;
my $size = $stop-$start+1;
warn "Feature $group ($size) is larger than $MAX_BIN. You will have trouble retrieving this feature.\nRerun script with --maxfeature set to a higher power of 10.\n" if $size > $MAX_BIN;
$source = '\N' unless defined $source;
$score = '\N' if $score eq '.';
$strand = '\N' if $strand eq '.';
$phase = '\N' if $phase eq '.';
my ($group_class,$group_name,$target_start,$target_stop,$attributes) = $db->split_group($group,$gff3);
# GFF2/3 transition
$group_class = [$group_class] unless ref $group_class;
$group_name = [$group_name] unless ref $group_name;
for (my $i=0; $i < @$group_name; $i++) {
$group_class->[$i] ||= '\N';
$group_name->[$i] ||= '\N';
$target_start ||= '\N';
$target_stop ||= '\N';
$method ||= '\N';
$source ||= '\N';
my $fid = $FID++;
my $gid = $GROUPID{lc join('',$group_class->[$i],$group_name->[$i])} ||= $GID++;
my $ftypeid = $FTYPEID{lc join('',$source,$method)} ||= $FTYPEID++;
my $bin = bin($start,$stop,$db->min_bin);
$FH{ FDATA() }->print( join("\t",$fid,$ref,$start,$stop,$bin,$ftypeid,$score,$strand,$phase,$gid,$target_start,$target_stop),"\n" );
if ($use_mysqlcmap){
my $feature_id = next_number(
db => $cmap_db,
table_name => 'cmap_feature',
id_field => 'feature_id',
)
or die 'No feature id';
my $direction = $strand eq '-' ? -1:1;
$FH{ FGROUP() }->print(
join("\t",$feature_id,$feature_id,'NULL',0, $group_name->[$i],0,0,'NULL',1,$direction, $group_class->[$i],)
,"\n"
) unless $DONE{"G$gid"}++;
}
else {
$FH{ FGROUP() }->print( join("\t",$gid,$group_class->[$i],$group_name->[$i]),"\n") unless $DONE{"G$gid"}++;
}
$FH{ FTYPE() }->print( join("\t",$ftypeid,$method,$source),"\n" ) unless $DONE{"T$ftypeid"}++;
foreach (@$attributes) {
my ($key,$value) = @$_;
my $attributeid = $ATTRIBUTEID{$key} ||= $ATTRIBUTEID++;
$FH{ FATTRIBUTE() }->print( join("\t",$attributeid,$key),"\n" ) unless $DONE{"A$attributeid"}++;
$FH{ FATTRIBUTE_TO_FEATURE() }->print( join("\t",$fid,$attributeid,$value),"\n");
}
if ( $fid % 1000 == 0) {
my $now = Time::HiRes::time() if $timer;
my $elapsed = $timer ? sprintf(" in %5.2fs",$now - $last) : '';
$last = $now;
print STDERR "$fid features parsed$elapsed...";
print STDERR -t STDOUT && !$ENV{EMACS} ? "\r" : "\n";
}
}
}
$FH{FASTA}->close if exists $FH{FASTA};
for my $file (@fasta) {
warn "Preparing DNA file $file....\n";
if ($use_pg){
$FH{FDNA() }->print("COPY fdna (fref, foffset, fdna) FROM stdin;\n");
}
my $old = select($FH{FDNA()});
$db->load_fasta($file) or warn "Couldn't load fasta file $file: $!";
if ($use_pg){
$FH{FDNA() }->print("\\.\n\n");
}
warn "done...\n";
select $old;
unlink $file if $tmpfiles{$file};
}
if ($use_pg) {
$FH{FDATA() }->print("\\.\n\n");
$FH{FTYPE() }->print("\\.\n\n");
$FH{FGROUP() }->print("\\.\n\n");
$FH{FATTRIBUTE() }->print("\\.\n\n");
$FH{FATTRIBUTE_TO_FEATURE()}->print("\\.\n\n");
}
$_->close foreach values %FH;
printf STDERR "Total parse time %5.2fs\n",(Time::HiRes::time() - $start) if $timer;
warn "Loading feature data and analyzing tables. You may see RDBMS messages here...\n";
if ($use_pg){
warn "Loading feature data. You may see Postgres comments...\n";
foreach (@files) {
my $file = "$tmpdir/$_.$$";
$AUTH ? system("psql $AUTH -f $file $DBNAME")
: system('psql','-f', $file, $DBNAME);
unlink $file;
}
warn "Updating sequences ...\n";
$db->update_sequences();
warn "Creating indexes ...\n";
$db->_create_indexes_etc();
warn "done...\n";
}
elsif( $use_mysql or $use_mysqlcmap ) {
$start = time();
my $success = 1;
my $TERMINATEDBY = $bWINDOWS ? q( LINES TERMINATED BY '\r\n') : '';
for my $f (@files) {
my $table = function_to_table($f,$ADAPTOR);
my $sql = join ('; ',
"lock tables $table write",
"delete from $table",
"load data $LOCAL infile '$tmpdir/$f.$$' replace into table $table $TERMINATEDBY",
"unlock tables");
my $command = MYSQL . qq[$AUTH -s -e "$sql"];
$command =~ s/\n/ /g;
$success &&= system($command) == 0;
unlink "$tmpdir/$f.$$";
}
printf STDERR "Total load time %5.2fs\n",(time() - $start) if $timer;
print STDERR "done...\n";
print STDERR "Analyzing/optimizing tables. You will see database messages...\n";
$start = time();
my $sql = '';
for my $f (@files) {
my $table = function_to_table($f,$ADAPTOR);
$sql .= "analyze table $table;";
}
my $command = MYSQL . qq[$AUTH -N -s -e "$sql"];
$success &&= system($command) == 0;
printf STDERR "Optimization time time %5.2fs\n",(time() - $start);
if ($success) {
print "$FEATURES features successfully loaded\n";
} else {
print "FAILURE: Please see standard error for details\n";
exit -1;
}
}
foreach (@fasta_files_to_be_unlinked) {
unlink "$tmpdir/$_.$$";
}
warn "Building summary statistics for coverage histograms...\n";
my (@args,$AUTH);
if (defined $USER) {
push @args,(-user=>$USER);
$AUTH .= " -u$USER";
}
if (defined $PASSWORD) {
push @args,(-pass=>$PASSWORD);
$AUTH .= " -p$PASSWORD";
}
push @args,(-preferred_groups=>[split(/[,\s+]+/,$GROUP_TAG)]) if defined $GROUP_TAG;
my $db = Bio::DB::GFF->new(-adaptor=>"dbi::$ADAPTOR",-dsn => $DSN,@args)
or die "Can't open database: ",Bio::DB::GFF->error,"\n";
$db->build_summary_statistics;
exit 0;
sub function_to_table {
my $function = shift;
my $adaptor = shift;
if ($function eq 'fdata'){
return 'fdata';
}
elsif ($function eq 'ftype'){
return 'ftype';
}
elsif ($function eq 'fgroup'){
return 'cmap_feature' if ($adaptor eq 'mysqlcmap');
return 'fgroup';
}
elsif ($function eq 'fdna'){
return 'fdna';
}
elsif ($function eq 'fattribute'){
return 'fattribute';
}
elsif ($function eq 'fattribute_to_feature'){
return 'fattribute_to_feature';
}
return '';
}
__END__
|